PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT5G06960.1

Common Name

BZIP26, MOJ9.13, OBF5, TGA5

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

bZIP

Protein Properties

Length: 330aa MW: 36890.4 Da PI: 8.9213

Description

OCS-element binding factor 5

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT5G06960.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	bZIP_1	30.7	6.6e-10	45	86	4	45
XCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH CS bZIP_1 4 lkrerrkqkNReAArrsRqRKkaeieeLeekvkeLeaeNkaL 45 k rr+++NReAAr+sR+RKka++++Le+ +L++ ++L AT5G06960.1 45 QKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLTQLEQEL 86 5888*************************9877777766555 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:1.20.5.170	9.3E-8	39	89	No hit	No description
SMART	SM00338	1.9E-7	42	105	IPR004827	Basic-leucine zipper domain
PROSITE profile	PS50217	9.438	44	88	IPR004827	Basic-leucine zipper domain
Pfam	PF00170	6.4E-7	46	86	IPR004827	Basic-leucine zipper domain
SuperFamily	SSF57959	5.56E-7	46	89	No hit	No description
PROSITE pattern	PS00036	0	49	64	IPR004827	Basic-leucine zipper domain
Pfam	PF14144	2.7E-32	128	204	IPR025422	Transcription factor TGA like domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009410	Biological Process	response to xenobiotic stimulus
GO:0009814	Biological Process	defense response, incompatible interaction
GO:0009863	Biological Process	salicylic acid mediated signaling pathway
GO:0005634	Cellular Component	nucleus
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0042802	Molecular Function	identical protein binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0008019	anatomy	leaf lamina base
PO:0009005	anatomy	root
PO:0009006	anatomy	shoot system
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0025281	anatomy	pollen
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007064	developmental stage	LP.12 twelve leaves visible stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 330 aa Download sequence Send to blast
MGDTSPRTSV STDGDTDHNN LMFDEGHLGI GASDSSDRSK SKMDQKTLRR LAQNREAARK 60 SRLRKKAYVQ QLENSRLKLT QLEQELQRAR QQGVFISSSG DQAHSTAGDG AMAFDVEYRR 120 WQEDKNRQMK ELSSAIDSHA TDSELRIIVD GVIAHYEELY RIKGNAAKSD VFHLLSGMWK 180 TPAERCFLWL GGFRSSELLK LIASQLEPLT EQQSLDINNL QQSSQQAEDA LSQGMDNLQQ 240 SLADTLSSGT LGSSSSGNVA SYMGQMAMAM GKLGTLEGFI RQADNLRLQT YQQMVRLLTT 300 RQSARALLAV HNYTLRLRAL SSLWLARPRE

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.5408	0.0	flower\| root\| silique

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	145357726	0.0
Genevisible	250655_at	0.0
Expression Atlas	AT5G06960	-
AtGenExpress	AT5G06960	-
ATTED-II	AT5G06960	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Predominantly expressed in roots.

Functional Description ? help Back to Top
Source	Description
TAIR	Encodes a basic leucine zipper (B-ZIP) containing protein that interacts with NPR1 to promote expression of salicylic acid induced genes. Binds the ocs-element.
UniProt	Transcriptional activator that binds specifically to the DNA sequence 5'-TGACG-3'. Recognizes ocs elements like the as-1 motif of the cauliflower mosaic virus 35S promoter. Binding to the as-1-like cis elements mediate auxin- and salicylic acid-inducible transcription. May be involved in the induction of the systemic acquired resistance (SAR) via its interaction with NPR1. Could also bind to the Hex-motif (5'-TGACGTGG-3') another cis-acting element found in plant histone promoters.

Function -- GeneRIF ? help Back to Top
data show TGA factors, which are essential for the salicylic acid (SA)-dependent establishment of systemic acquired resistance, play a pivotal role in the activation of the jasmonic acid/ethylene pathway, both after pathogen infection & hormone treatment [PMID: 19832945]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00493	DAP	27203113	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT5G06960.1

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source	Target Gene (A: Activate/R: Repress)
ATRM	AT2G14610(A), AT5G44420(R)

Regulation -- Hormone ? help Back to Top
Source	Hormone
AHD	salicylic acid

Interaction ? help Back to Top
Source	Intact With
BioGRID	AT5G06960
IntAct	Search Q39163

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT5G06960

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AY058830	0.0	AY058830.1 Arabidopsis thaliana AT5g06960/MOJ9_13 mRNA, complete cds.
GenBank	AY143864	0.0	AY143864.1 Arabidopsis thaliana At5g06960/MOJ9_13 mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_196313.1	0.0	OCS-element binding factor 5
Refseq	NP_974745.1	0.0	OCS-element binding factor 5
Swissprot	Q39163	0.0	TGA5_ARATH; Transcription factor TGA5
TrEMBL	A0A178UIF0	0.0	A0A178UIF0_ARATH; TGA5
STRING	AT5G06960.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM667	28	133
Representative plant	OGRP722	16	67

Link Out ? help Back to Top
Phytozome	AT5G06960.1
Entrez Gene	830587
iHOP	AT5G06960
wikigenes	AT5G06960

Publications ? help Back to Top
Zhang Y,Fan W,Kinkema M,Li X,Dong X Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene. Proc. Natl. Acad. Sci. U.S.A., 1999. 96(11): p. 6523-8 [PMID:10339621] Zhou JM, et al. NPR1 differentially interacts with members of the TGA/OBF family of transcription factors that bind an element of the PR-1 gene required for induction by salicylic acid. Mol. Plant Microbe Interact., 2000. 13(2): p. 191-202 [PMID:10659709] Després C,DeLong C,Glaze S,Liu E,Fobert PR The Arabidopsis NPR1/NIM1 protein enhances the DNA binding activity of a subgroup of the TGA family of bZIP transcription factors. Plant Cell, 2000. 12(2): p. 279-90 [PMID:10662863] Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Jakoby M, et al. bZIP transcription factors in Arabidopsis. Trends Plant Sci., 2002. 7(3): p. 106-11 [PMID:11906833] Kim HS,Delaney TP Over-expression of TGA5, which encodes a bZIP transcription factor that interacts with NIM1/NPR1, confers SAR-independent resistance in Arabidopsis thaliana to Peronospora parasitica. Plant J., 2002. 32(2): p. 151-63 [PMID:12383081] Zhang Y,Tessaro MJ,Lassner M,Li X Knockout analysis of Arabidopsis transcription factors TGA2, TGA5, and TGA6 reveals their redundant and essential roles in systemic acquired resistance. Plant Cell, 2003. 15(11): p. 2647-53 [PMID:14576289] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Liu G,Holub EB,Alonso JM,Ecker JR,Fobert PR An Arabidopsis NPR1-like gene, NPR4, is required for disease resistance. Plant J., 2005. 41(2): p. 304-18 [PMID:15634206] Kang HG,Klessig DF Salicylic acid-inducible Arabidopsis CK2-like activity phosphorylates TGA2. Plant Mol. Biol., 2005. 57(4): p. 541-57 [PMID:15821979] Zhang Y, et al. Negative regulation of defense responses in Arabidopsis by two NPR1 paralogs. Plant J., 2006. 48(5): p. 647-56 [PMID:17076807] Churchman ML, et al. SIAMESE, a plant-specific cell cycle regulator, controls endoreplication onset in Arabidopsis thaliana. Plant Cell, 2006. 18(11): p. 3145-57 [PMID:17098811] Rochon A,Boyle P,Wignes T,Fobert PR,Després C The coactivator function of Arabidopsis NPR1 requires the core of its BTB/POZ domain and the oxidation of C-terminal cysteines. Plant Cell, 2006. 18(12): p. 3670-85 [PMID:17172357] Ndamukong I, et al. SA-inducible Arabidopsis glutaredoxin interacts with TGA factors and suppresses JA-responsive PDF1.2 transcription. Plant J., 2007. 50(1): p. 128-39 [PMID:17397508] Mueller S, et al. General detoxification and stress responses are mediated by oxidized lipids through TGA transcription factors in Arabidopsis. Plant Cell, 2008. 20(3): p. 768-85 [PMID:18334669] Manzano C,Abraham Z,L Identification of ubiquitinated proteins in Arabidopsis. Plant Mol. Biol., 2008. 68(1-2): p. 145-58 [PMID:18535787] Ascencio-Ib Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection. Plant Physiol., 2008. 148(1): p. 436-54 [PMID:18650403] Fode B,Siemsen T,Thurow C,Weigel R,Gatz C The Arabidopsis GRAS protein SCL14 interacts with class II TGA transcription factors and is essential for the activation of stress-inducible promoters. Plant Cell, 2008. 20(11): p. 3122-35 [PMID:18984675] Li S, et al. Nuclear activity of ROXY1, a glutaredoxin interacting with TGA factors, is required for petal development in Arabidopsis thaliana. Plant Cell, 2009. 21(2): p. 429-41 [PMID:19218396] Zander M,La Camera S,Lamotte O,Métraux JP,Gatz C Arabidopsis thaliana class-II TGA transcription factors are essential activators of jasmonic acid/ethylene-induced defense responses. Plant J., 2010. 61(2): p. 200-10 [PMID:19832945] Matthes MC, et al. The transcriptome of cis-jasmone-induced resistance in Arabidopsis thaliana and its role in indirect defence. Planta, 2010. 232(5): p. 1163-80 [PMID:20711606] Arabidopsis Interactome Mapping Consortium Evidence for network evolution in an Arabidopsis interactome map. Science, 2011. 333(6042): p. 601-7 [PMID:21798944] Causier B,Ashworth M,Guo W,Davies B The TOPLESS interactome: a framework for gene repression in Arabidopsis. Plant Physiol., 2012. 158(1): p. 423-38 [PMID:22065421] Zander M,Chen S,Imkampe J,Thurow C,Gatz C Repression of the Arabidopsis thaliana jasmonic acid/ethylene-induced defense pathway by TGA-interacting glutaredoxins depends on their C-terminal ALWL motif. Mol Plant, 2012. 5(4): p. 831-40 [PMID:22207719] Heyndrickx KS,Vandepoele K Systematic identification of functional plant modules through the integration of complementary data sources. Plant Physiol., 2012. 159(3): p. 884-901 [PMID:22589469] Efroni I, et al. Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses. Dev. Cell, 2013. 24(4): p. 438-45 [PMID:23449474] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Zander M,Thurow C,Gatz C TGA Transcription Factors Activate the Salicylic Acid-Suppressible Branch of the Ethylene-Induced Defense Program by Regulating ORA59 Expression. Plant Physiol., 2014. 165(4): p. 1671-1683 [PMID:24989234] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178] Walper E,Weiste C,Mueller MJ,Hamberg M,Dröge-Laser W Screen Identifying Arabidopsis Transcription Factors Involved in the Response to 9-Lipoxygenase-Derived Oxylipins. PLoS ONE, 2016. 11(4): p. e0153216 [PMID:27073862] Huang LJ, et al. Ectopically expressed glutaredoxin ROXY19 negatively regulates the detoxification pathway in Arabidopsis thaliana. BMC Plant Biol., 2016. 16(1): p. 200 [PMID:27624344] Lam E,Lam YK Binding site requirements and differential representation of TGF factors in nuclear ASF-1 activity. Nucleic Acids Res., 1995. 23(18): p. 3778-85 [PMID:7479010] Zhang B,Foley RC,Singh KB Isolation and characterization of two related Arabidopsis ocs-element bZIP binding proteins. Plant J., 1993. 4(4): p. 711-6 [PMID:8252072] Zhang B,Chen W,Foley RC,Büttner M,Singh KB Interactions between distinct types of DNA binding proteins enhance binding to ocs element promoter sequences. Plant Cell, 1995. 7(12): p. 2241-52 [PMID:8718629] Xiang C,Miao Z,Lam E DNA-binding properties, genomic organization and expression pattern of TGA6, a new member of the TGA family of bZIP transcription factors in Arabidopsis thaliana. Plant Mol. Biol., 1997. 34(3): p. 403-15 [PMID:9225852]