PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT4G36730.2

Common Name

AtGBF1, BZIP41, C7A10_630, GBF1

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

bZIP

Protein Properties

Length: 313aa MW: 33675 Da PI: 6.6885

Description

G-box binding factor 1

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT4G36730.2	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	bZIP_1	78.1	1.1e-24	218	280	1	63
XXXXCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH CS bZIP_1 1 ekelkrerrkqkNReAArrsRqRKkaeieeLeekvkeLeaeNkaLkkeleelkkevaklksev 63 e+elkr++rkq+NRe+ArrsR+RK+ae+e+L+++v++L++eN++L++el++l+ e++klkse+ AT4G36730.2 218 ERELKRQKRKQSNRESARRSRLRKQAECEQLQQRVESLSNENQSLRDELQRLSSECDKLKSEN 280 89***********************************************************98 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF07777	4.6E-30	1	94	IPR012900	G-box binding protein, multifunctional mosaic region
Gene3D	G3DSA:1.20.5.170	3.1E-19	215	278	No hit	No description
SMART	SM00338	3.8E-23	218	282	IPR004827	Basic-leucine zipper domain
Pfam	PF00170	1.1E-22	218	280	IPR004827	Basic-leucine zipper domain
PROSITE profile	PS50217	13.817	220	283	IPR004827	Basic-leucine zipper domain
SuperFamily	SSF57959	4.15E-12	221	278	No hit	No description
CDD	cd14702	3.82E-24	223	274	No hit	No description
PROSITE pattern	PS00036	0	225	240	IPR004827	Basic-leucine zipper domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0010310	Biological Process	regulation of hydrogen peroxide metabolic process
GO:0010629	Biological Process	negative regulation of gene expression
GO:0090342	Biological Process	regulation of cell aging
GO:0005634	Cellular Component	nucleus
GO:0005737	Cellular Component	cytoplasm
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding
GO:0044212	Molecular Function	transcription regulatory region DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0008019	anatomy	leaf lamina base
PO:0009005	anatomy	root
PO:0009006	anatomy	shoot system
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0025281	anatomy	pollen
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007064	developmental stage	LP.12 twelve leaves visible stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 313 aa Download sequence Send to blast
MGTSEDKMPF KTTKPTSSAQ EVPPTPYPDW QNSMQAYYGG GGTPNPFFPS PVGSPSPHPY 60 MWGAQHHMMP PYGTPVPYPA MYPPGAVYAH PSMPMPPNSG PTNKEPAKDQ ASGKKSKGNS 120 KKKAEGGDKA LSGSGNDGAS HSDESVTAGS SDENDENANQ QGSIRKPSFG QMLADASSQS 180 TTGEIQGSVP MKPVAPGTNL NIGMDLWSSQ AGVPVKDERE LKRQKRKQSN RESARRSRLR 240 KQAECEQLQQ RVESLSNENQ SLRDELQRLS SECDKLKSEN NSIQDELQRV LGAEAVANLE 300 QNAAGSKDGE GTN

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	234	240	RRSRLRK
2	234	241	RRSRLRKQ

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.62	0.0	flower\| leaf\| root\| seed

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	145354367	0.0
Genevisible	246211_at	0.0
Expression Atlas	AT4G36730	-
AtGenExpress	AT4G36730	-
ATTED-II	AT4G36730	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Found in both light and dark grown leaves.

Functional Description ? help Back to Top
Source	Description
TAIR	member of a gene family encoding basic leucine zipper proteins (GBFs) which bind the G-box
UniProt	Binds to the G-box motif (5'-CCACGTGG-3') of the rbcS-1A gene promoter (PubMed:1373374). G-box and G-box-like motifs are cis-acting elements defined in promoters of certain plant genes which are regulated by such diverse stimuli as light-induction or hormone control. Binds to the G-box motif 5'-CACGTG-3' of LHCB2.4 (At3g27690) promoter. May act as transcriptional activator in light-regulated expression of LHCB2.4. Probably binds DNA as monomer. DNA-binding activity is redox-dependent (PubMed:22718771). {ECO:0000269\|PubMed:1373374, ECO:0000269\|PubMed:22718771}.

Function -- GeneRIF ? help Back to Top
GBF1, a transcription factor of blue light signaling in Arabidopsis, is degraded in the dark by a proteasome-mediated pathway independent of COP1 and SPA1 [PMID: 18930926] In Arabidopsis, GBF1-related exchange factors for the ARF GTPases (ARF GEFs) GNOM and GNL2 play essential roles in polar tip growth of root hairs and pollen, respectively. [PMID: 22138577] the functional interrelations of GBF1 with HY5 and HYH in Arabidopsis seedling development. [PMID: 22692212] Genetic interactions of HY1 and GBF1 in seedling photomorphogenesis. [PMID: 22766018] The DNA binding activity of GBF1 is modulated by heterodimeriation with HY5 and HYH. [PMID: 24157608] GBF1 is a transcriptional repressor of the AtCathB3 gene and affects germination kinetics of A. thaliana seeds. As AtCathB3 is also expressed during post-germination in cotyledons, a role for the AtCathB3-like protease in reserve mobilization is inferred. [PMID: 24600022] This study reveals that MYC2 and GBF1 colocalize and physically interact in the nucleus. This interaction requires the N-terminal domain of each protein. [PMID: 26047210]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00039	PBM	25215497	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT4G36730.2

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source	Target Gene (A: Activate/R: Repress)
ATRM	AT1G29930(A), AT4G10040(A), AT4G35090(R)

Interaction ? help Back to Top
Source	Intact With
BioGRID	AT5G11260, AT5G44190, AT1G32640
IntAct	Search P42774

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT4G36730

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AF428382	0.0	AF428382.1 Arabidopsis thaliana At4g37294/C7A10_630 mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_849510.1	0.0	G-box binding factor 1
Swissprot	P42774	0.0	GBF1_ARATH; G-box-binding factor 1
TrEMBL	A0A384KK13	0.0	A0A384KK13_ARATH; GBF1
TrEMBL	Q5K1L6	0.0	Q5K1L6_ARATH; Z-box binding factor 2 protein
STRING	AT4G36730.1	0.0	(Arabidopsis thaliana)

Link Out ? help Back to Top
Phytozome	AT4G36730.2
Entrez Gene	829826
iHOP	AT4G36730
wikigenes	AT4G36730

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Tamai H,Iwabuchi M,Meshi T Arabidopsis GARP transcriptional activators interact with the Pro-rich activation domain shared by G-box-binding bZIP factors. Plant Cell Physiol., 2002. 43(1): p. 99-107 [PMID:11828027] Jakoby M, et al. bZIP transcription factors in Arabidopsis. Trends Plant Sci., 2002. 7(3): p. 106-11 [PMID:11906833] Schindler U,Menkens AE,Beckmann H,Ecker JR,Cashmore AR Heterodimerization between light-regulated and ubiquitously expressed Arabidopsis GBF bZIP proteins. EMBO J., 1992. 11(4): p. 1261-73 [PMID:1373374] Schindler U,Beckmann H,Cashmore AR TGA1 and G-box binding factors: two distinct classes of Arabidopsis leucine zipper proteins compete for the G-box-like element TGACGTGG. Plant Cell, 1992. 4(10): p. 1309-19 [PMID:1446171] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Klimczak LJ,Schindler U,Cashmore AR DNA binding activity of the Arabidopsis G-box binding factor GBF1 is stimulated by phosphorylation by casein kinase II from broccoli. Plant Cell, 1992. 4(1): p. 87-98 [PMID:1525562] Schindler U,Terzaghi W,Beckmann H,Kadesch T,Cashmore AR DNA binding site preferences and transcriptional activation properties of the Arabidopsis transcription factor GBF1. EMBO J., 1992. 11(4): p. 1275-89 [PMID:1563344] Sehnke PC,Laughner BJ,Lyerly Linebarger CR,Gurley WB,Ferl RJ Identification and characterization of GIP1, an Arabidopsis thaliana protein that enhances the DNA binding affinity and reduces the oligomeric state of G-box binding factors. Cell Res., 2005. 15(8): p. 567-75 [PMID:16117846] Mallappa C,Yadav V,Negi P,Chattopadhyay S A basic leucine zipper transcription factor, G-box-binding factor 1, regulates blue light-mediated photomorphogenic growth in Arabidopsis. J. Biol. Chem., 2006. 281(31): p. 22190-9 [PMID:16638747] Popescu SC, et al. Differential binding of calmodulin-related proteins to their targets revealed through high-density Arabidopsis protein microarrays. Proc. Natl. Acad. Sci. U.S.A., 2007. 104(11): p. 4730-5 [PMID:17360592] Shen H,Cao K,Wang X AtbZIP16 and AtbZIP68, two new members of GBFs, can interact with other G group bZIPs in Arabidopsis thaliana. BMB Rep, 2008. 41(2): p. 132-8 [PMID:18315949] Mallappa C,Singh A,Ram H,Chattopadhyay S GBF1, a transcription factor of blue light signaling in Arabidopsis, is degraded in the dark by a proteasome-mediated pathway independent of COP1 and SPA1. J. Biol. Chem., 2008. 283(51): p. 35772-82 [PMID:18930926] Welchen E, et al. A segment containing a G-box and an ACGT motif confers differential expression characteristics and responses to the Arabidopsis Cytc-2 gene, encoding an isoform of cytochrome c. J. Exp. Bot., 2009. 60(3): p. 829-45 [PMID:19098132] Nozawa A, et al. Construction of a protein library of Arabidopsis transcription factors using a wheat cell-free protein production system and its application for DNA binding analysis. Biosci. Biotechnol. Biochem., 2009. 73(7): p. 1661-4 [PMID:19584551] Smykowski A,Zimmermann P,Zentgraf U G-Box binding factor1 reduces CATALASE2 expression and regulates the onset of leaf senescence in Arabidopsis. Plant Physiol., 2010. 153(3): p. 1321-31 [PMID:20484024] Gaudinier A, et al. Enhanced Y1H assays for Arabidopsis. Nat. Methods, 2011. 8(12): p. 1053-5 [PMID:22037706] Richter S, et al. Polarized cell growth in Arabidopsis requires endosomal recycling mediated by GBF1-related ARF exchange factors. Nat. Cell Biol., 2012. 14(1): p. 80-6 [PMID:22138577] Singh A,Ram H,Abbas N,Chattopadhyay S Molecular interactions of GBF1 with HY5 and HYH proteins during light-mediated seedling development in Arabidopsis thaliana. J. Biol. Chem., 2012. 287(31): p. 25995-6009 [PMID:22692212] Shaikhali J, et al. Redox-mediated mechanisms regulate DNA binding activity of the G-group of basic region leucine zipper (bZIP) transcription factors in Arabidopsis. J. Biol. Chem., 2012. 287(33): p. 27510-25 [PMID:22718771] Prasad VB,Gupta N,Nandi A,Chattopadhyay S HY1 genetically interacts with GBF1 and regulates the activity of the Z-box containing promoters in light signaling pathways in Arabidopsis thaliana. Mech. Dev., 2012 Sep-Dec. 129(9-12): p. 298-307 [PMID:22766018] Ram H,Chattopadhyay S Molecular interaction of bZIP domains of GBF1, HY5 and HYH in Arabidopsis seedling development. Plant Signal Behav, 2013. 8(1): p. e22703 [PMID:23123453] Gangappa SN,Srivastava AK,Maurya JP,Ram H,Chattopadhyay S Z-box binding transcription factors (ZBFs): a new class of transcription factors in Arabidopsis seedling development. Mol Plant, 2013. 6(6): p. 1758-68 [PMID:24157607] Ram H,Priya P,Jain M,Chattopadhyay S Genome-wide DNA binding of GBF1 is modulated by its heterodimerizing protein partners, HY5 and HYH. Mol Plant, 2014. 7(2): p. 448-51 [PMID:24157608] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Iglesias-Fern The AtCathB3 gene, encoding a cathepsin B-like protease, is expressed during germination of Arabidopsis thaliana and transcriptionally repressed by the basic leucine zipper protein GBF1. J. Exp. Bot., 2014. 65(8): p. 2009-21 [PMID:24600022] Shaikhali J GIP1 protein is a novel cofactor that regulates DNA-binding affinity of redox-regulated members of bZIP transcription factors involved in the early stages of Arabidopsis development. Protoplasma, 2015. 252(3): p. 867-83 [PMID:25387999] Karumuri S,Bandopadhyay R In silico analysis of the structure and interaction of COP1 protein of Arabidopsis thaliana. Indian J. Biochem. Biophys., 2014. 51(5): p. 343-9 [PMID:25630103] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178] Maurya JP,Sethi V,Gangappa SN,Gupta N,Chattopadhyay S Interaction of MYC2 and GBF1 results in functional antagonism in blue light-mediated Arabidopsis seedling development. Plant J., 2015. 83(3): p. 439-50 [PMID:26047210] Smykowski A,Zimmermann P,Zentgraf U CORRECTION. G-Box Binding Factor1 Reduces CATALASE2 Expression and Regulates the Onset of Leaf Senescence in Arabidopsis. Plant Physiol., 2016. 170(2): p. 1164-7 [PMID:26826725] Smykowski A,Fischer SM,Zentgraf U Phosphorylation Affects DNA-Binding of the Senescence-Regulating bZIP Transcription Factor GBF1. Plants (Basel), 2015. 4(3): p. 691-709 [PMID:27135347] Giri MK, et al. GBF1 differentially regulates CAT2 and PAD4 transcription to promote pathogen defense in Arabidopsis thaliana. Plant J., 2017. 91(5): p. 802-815 [PMID:28622438] Klimczak LJ, et al. Reconstitution of Arabidopsis casein kinase II from recombinant subunits and phosphorylation of transcription factor GBF1. Plant Cell, 1995. 7(1): p. 105-15 [PMID:7696877] Terzaghi WB,Bertekap RL,Cashmore AR Intracellular localization of GBF proteins and blue light-induced import of GBF2 fusion proteins into the nucleus of cultured Arabidopsis and soybean cells. Plant J., 1997. 11(5): p. 967-82 [PMID:9193069]