PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT4G34000.3

Common Name

ABF3, AtABF3, DPBF5

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

bZIP

Protein Properties

Length: 449aa MW: 48905 Da PI: 6.9573

Description

abscisic acid responsive elements-binding factor 3

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT4G34000.3	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	bZIP_1	45.1	2.2e-14	374	418	5	49
CHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH CS bZIP_1 5 krerrkqkNReAArrsRqRKkaeieeLeekvkeLeaeNkaLkkel 49 +r++r++kNRe+A rsR+RK+a++ eLe ++++L++ N++L+k+ AT4G34000.3 374 RRQKRMIKNRESAARSRARKQAYTMELEAEIAQLKELNEELQKKQ 418 69***************************************9875 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SMART	SM00338	1.7E-10	370	430	IPR004827	Basic-leucine zipper domain
PROSITE profile	PS50217	10.955	372	429	IPR004827	Basic-leucine zipper domain
Pfam	PF00170	2.4E-12	374	429	IPR004827	Basic-leucine zipper domain
SuperFamily	SSF57959	7.5E-11	374	428	No hit	No description
CDD	cd14707	4.38E-28	374	428	No hit	No description
Gene3D	G3DSA:1.20.5.170	1.3E-14	374	429	No hit	No description
PROSITE pattern	PS00036	0	377	392	IPR004827	Basic-leucine zipper domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0009414	Biological Process	response to water deprivation
GO:0009651	Biological Process	response to salt stress
GO:0009738	Biological Process	abscisic acid-activated signaling pathway
GO:0045893	Biological Process	positive regulation of transcription, DNA-templated
GO:0005634	Cellular Component	nucleus
GO:0000976	Molecular Function	transcription regulatory region sequence-specific DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0005515	Molecular Function	protein binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000005	anatomy	cultured plant cell
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0008019	anatomy	leaf lamina base
PO:0009005	anatomy	root
PO:0009006	anatomy	shoot system
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0025281	anatomy	pollen
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 449 aa Download sequence Send to blast
MGSRLNFKSF VDGVSEQQPT VGTSLPLTRQ NSVFSLTFDE FQNSWGGGIG KDFGSMNMDE 60 LLKNIWTAEE SHSMMGNNTS YTNISNGNSG NTVINGGGNN IGGLAVGVGG ESGGFFTGGS 120 LQRQGSLTLP RTISQKRVDD VWKELMKEDD IGNGVVNGGT SGIPQRQQTL GEMTLEEFLV 180 RAGVVREEPQ PVESVTNFNG GFYGFGSNGG LGTASNGFVA NQPQDLSGNG VAVRQDLLTA 240 QTQPLQMQQP QMVQQPQMVQ QPQQLIQTQE RPFPKQTTIA FSNTVDVVNR SQPATQCQEV 300 KPSILGIHNH PMNNNLLQAV DFKTGVTVAA VSPGSQMSPD LTPKSALDAS LSPVPYMFGR 360 VRKTGAVLEK VIERRQKRMI KNRESAARSR ARKQAYTMEL EAEIAQLKEL NEELQKKQVC 420 LASSLSQLRI SRFSYFLEVV FTDQMFHAG

Sequence ? help Back to Top

Protein Sequence Length: 449 aa Download sequence Send to blast

MGSRLNFKSF VDGVSEQQPT VGTSLPLTRQ NSVFSLTFDE FQNSWGGGIG KDFGSMNMDE  60
LLKNIWTAEE SHSMMGNNTS YTNISNGNSG NTVINGGGNN IGGLAVGVGG ESGGFFTGGS  120
LQRQGSLTLP RTISQKRVDD VWKELMKEDD IGNGVVNGGT SGIPQRQQTL GEMTLEEFLV  180
RAGVVREEPQ PVESVTNFNG GFYGFGSNGG LGTASNGFVA NQPQDLSGNG VAVRQDLLTA  240
QTQPLQMQQP QMVQQPQMVQ QPQQLIQTQE RPFPKQTTIA FSNTVDVVNR SQPATQCQEV  300
KPSILGIHNH PMNNNLLQAV DFKTGVTVAA VSPGSQMSPD LTPKSALDAS LSPVPYMFGR  360
VRKTGAVLEK VIERRQKRMI KNRESAARSR ARKQAYTMEL EAEIAQLKEL NEELQKKQVC  420
LASSLSQLRI SRFSYFLEVV FTDQMFHAG

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.21769	0.0	flower\| root\| seed

Expression -- Microarray ? help Back to Top
Source	ID	E-value
Genevisible	253263_at	0.0
Expression Atlas	AT4G34000	-
AtGenExpress	AT4G34000	-
ATTED-II	AT4G34000	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed in roots and flowers. {ECO:0000269\|PubMed:11884679}.

Functional Description ? help Back to Top
Source	Description
TAIR	Encodes an ABA-responsive element-binding protein with similarity to transcription factors that is expressed in response to stress and abscisic acid.
UniProt	Binds to the ABA-responsive element (ABRE). Mediates stress-responsive ABA signaling. {ECO:0000269\|PubMed:11884679, ECO:0000269\|PubMed:15361142}.

Function -- GeneRIF ? help Back to Top
Makes transgenic plants more tolerant to stress conditions. [PMID: 15834008] The redundancy of function among genes in the families of ABF3 and ABI5 in A. thaliana is reported. [PMID: 16247556] AREB1, AREB2, and ABF3 are master transcription factors that regulate the ABRE-dependent expression of water-stress responsive genes in ABA signaling in response to water stresses in a cooperative manner. [PMID: 19947981] Overexpression of ABF3 has a minimal impact on the transcriptome, with differences in the gene expression pattern only detectable in response to drought. [PMID: 20105335] Data show that OST1 phosphorylates ABF3 on multiple LXRXXpS/T preferred motifs including T451 located in the midst of a conserved 14-3-3 binding site. [PMID: 21085673] ABF1 and ABF3 abundance is affected by ABA and KEG [PMID: 23742014] The abscisic acid feedback regulation of AtNCED3 expression is not mediated by ABF3. [PMID: 24475264] In this study, an attempt has been made to improve CT in drought hardy crop, peanut (Arachis hypogaea L., cv. TMV2) by co-expressing stress-responsive transcription factors (TFs), AtDREB2A, AtHB7 and AtABF3, associated with downstream gene expression [PMID: 25474740] In addition to phosphate starvation signaling, miR399f might also modulate plant responses to salt, ABA, and drought, by regulating the expression of newly discovered target genes, ABF3 and CSP41b. [PMID: 26674968] ABF2, ABF3, and ABF4 Promote ABA-Mediated Chlorophyll Degradation and Leaf Senescence by Transcriptional Activation of Chlorophyll Catabolic Genes and Senescence-Associated Genes in Arabidopsis [PMID: 27373216] Transgenic AtABF3 alfalfa plants might be useful for breeding forage crops with enhanced tolerance to environmental stress for use in sustainable agriculture on marginal lands. The transgenic plants exhibited enhanced drought stress tolerance and better growth under drought stress treatment, which was accompanied by a reduced transpiration rate and lower reactive oxygen species contents. [PMID: 27721135] the role of ABF1/3/4 in det1 germination phenotypes [PMID: 29680877] Overexpression of the AtABF3 gene conferred drought and salt tolerance in major crop soybean, especially under the growth condition of low watering. [PMID: 29754472]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00038	PBM	25215497	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT4G34000.3

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Up-regulated by drought, salt, abscisic acid (ABA). {ECO:0000269\|PubMed:10636868, ECO:0000269\|PubMed:16284313}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source	Upstream Regulator (A: Activate/R: Repress)
ATRM	AT3G24650 (R)

Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source	Target Gene (A: Activate/R: Repress)
ATRM	AT3G11410(A), AT3G12580(A), AT5G52300(A)

Regulation -- Hormone ? help Back to Top
Source	Hormone
AHD	abscisic acid

Interaction ? help Back to Top
Source	Intact With
BioGRID	AT4G34000, AT1G10585, AT1G45249, AT1G49720

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT4G34000

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AF334210	0.0	AF334210.1 Arabidopsis thaliana bZIP protein DPBF5 mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_001031785.2	0.0	abscisic acid responsive elements-binding factor 3
Swissprot	Q9M7Q3	0.0	AI5L6_ARATH; ABSCISIC ACID-INSENSITIVE 5-like protein 6
TrEMBL	A0A178UYI8	0.0	A0A178UYI8_ARATH; DPBF5
STRING	AT4G34000.1	0.0	(Arabidopsis thaliana)

Link Out ? help Back to Top
Phytozome	AT4G34000.3
Entrez Gene	829547
iHOP	AT4G34000
wikigenes	AT4G34000

Publications ? help Back to Top
Choi H,Hong J,Ha J,Kang J,Kim SY ABFs, a family of ABA-responsive element binding factors. J. Biol. Chem., 2000. 275(3): p. 1723-30 [PMID:10636868] Finkelstein RR,Lynch TJ The Arabidopsis abscisic acid response gene ABI5 encodes a basic leucine zipper transcription factor. Plant Cell, 2000. 12(4): p. 599-609 [PMID:10760247] Riechmann JL,Ratcliffe OJ A genomic perspective on plant transcription factors. Curr. Opin. Plant Biol., 2000. 3(5): p. 423-34 [PMID:11019812] Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Kang JY,Choi HI,Im MY,Kim SY Arabidopsis basic leucine zipper proteins that mediate stress-responsive abscisic acid signaling. Plant Cell, 2002. 14(2): p. 343-57 [PMID:11884679] Jakoby M, et al. bZIP transcription factors in Arabidopsis. Trends Plant Sci., 2002. 7(3): p. 106-11 [PMID:11906833] Kim SY,Ma J,Perret P,Li Z,Thomas TL Arabidopsis ABI5 subfamily members have distinct DNA-binding and transcriptional activities. Plant Physiol., 2002. 130(2): p. 688-97 [PMID:12376636] Suzuki M,Ketterling MG,Li QB,McCarty DR Viviparous1 alters global gene expression patterns through regulation of abscisic acid signaling. Plant Physiol., 2003. 132(3): p. 1664-77 [PMID:12857845] Oh SJ, et al. Arabidopsis CBF3/DREB1A and ABF3 in transgenic rice increased tolerance to abiotic stress without stunting growth. Plant Physiol., 2005. 138(1): p. 341-51 [PMID:15834008] Finkelstein R,Gampala SS,Lynch TJ,Thomas TL,Rock CD Redundant and distinct functions of the ABA response loci ABA-INSENSITIVE(ABI)5 and ABRE-BINDING FACTOR (ABF)3. Plant Mol. Biol., 2005. 59(2): p. 253-67 [PMID:16247556] Xin Z,Zhao Y,Zheng ZL Transcriptome analysis reveals specific modulation of abscisic acid signaling by ROP10 small GTPase in Arabidopsis. Plant Physiol., 2005. 139(3): p. 1350-65 [PMID:16258012] Choi HI, et al. Arabidopsis calcium-dependent protein kinase AtCPK32 interacts with ABF4, a transcriptional regulator of abscisic acid-responsive gene expression, and modulates its activity. Plant Physiol., 2005. 139(4): p. 1750-61 [PMID:16299177] Nakashima K, et al. Transcriptional regulation of ABI3- and ABA-responsive genes including RD29B and RD29A in seeds, germinating embryos, and seedlings of Arabidopsis. Plant Mol. Biol., 2006. 60(1): p. 51-68 [PMID:16463099] Li S,Xu C,Yang Y,Xia G Functional analysis of TaDi19A, a salt-responsive gene in wheat. Plant Cell Environ., 2010. 33(1): p. 117-29 [PMID:19895399] Yoshida T, et al. AREB1, AREB2, and ABF3 are master transcription factors that cooperatively regulate ABRE-dependent ABA signaling involved in drought stress tolerance and require ABA for full activation. Plant J., 2010. 61(4): p. 672-85 [PMID:19947981] Abdeen A,Schnell J,Miki B Transcriptome analysis reveals absence of unintended effects in drought-tolerant transgenic plants overexpressing the transcription factor ABF3. BMC Genomics, 2010. 11: p. 69 [PMID:20105335] Lee SJ, et al. DREB2C interacts with ABF2, a bZIP protein regulating abscisic acid-responsive gene expression, and its overexpression affects abscisic acid sensitivity. Plant Physiol., 2010. 153(2): p. 716-27 [PMID:20395451] Kline KG,Barrett-Wilt GA,Sussman MR In planta changes in protein phosphorylation induced by the plant hormone abscisic acid. Proc. Natl. Acad. Sci. U.S.A., 2010. 107(36): p. 15986-91 [PMID:20733066] Li G, et al. Two cotton Cys2/His2-type zinc-finger proteins, GhDi19-1 and GhDi19-2, are involved in plant response to salt/drought stress and abscisic acid signaling. Plant Mol. Biol., 2010. 74(4-5): p. 437-52 [PMID:20852918] Sirichandra C, et al. The Arabidopsis ABA-activated kinase OST1 phosphorylates the bZIP transcription factor ABF3 and creates a 14-3-3 binding site involved in its turnover. PLoS ONE, 2010. 5(11): p. e13935 [PMID:21085673] Kim YY,Jung KW,Yoo KS,Jeung JU,Shin JS A stress-responsive caleosin-like protein, AtCLO4, acts as a negative regulator of ABA responses in Arabidopsis. Plant Cell Physiol., 2011. 52(5): p. 874-84 [PMID:21471120] Kim JS, et al. An ABRE promoter sequence is involved in osmotic stress-responsive expression of the DREB2A gene, which encodes a transcription factor regulating drought-inducible genes in Arabidopsis. Plant Cell Physiol., 2011. 52(12): p. 2136-46 [PMID:22025559] He Y, et al. Ectopic expression of a wheat MYB transcription factor gene, TaMYB73, improves salinity stress tolerance in Arabidopsis thaliana. J. Exp. Bot., 2012. 63(3): p. 1511-22 [PMID:22140235] Qin Y, et al. Over-expression of TaMYB33 encoding a novel wheat MYB transcription factor increases salt and drought tolerance in Arabidopsis. Mol. Biol. Rep., 2012. 39(6): p. 7183-92 [PMID:22350156] Lynch T,Erickson BJ,Finkelstein RR Direct interactions of ABA-insensitive(ABI)-clade protein phosphatase(PP)2Cs with calcium-dependent protein kinases and ABA response element-binding bZIPs may contribute to turning off ABA response. Plant Mol. Biol., 2012. 80(6): p. 647-58 [PMID:23007729] Efroni I, et al. Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses. Dev. Cell, 2013. 24(4): p. 438-45 [PMID:23449474] Chen YT,Liu H,Stone S,Callis J ABA and the ubiquitin E3 ligase KEEP ON GOING affect proteolysis of the Arabidopsis thaliana transcription factors ABF1 and ABF3. Plant J., 2013. 75(6): p. 965-76 [PMID:23742014] Zhao Q, et al. Regulation and function of Arabidopsis AtGALK2 gene in abscisic acid response signaling. Mol. Biol. Rep., 2018. [PMID:24078097] Bao Y, et al. The tumor necrosis factor receptor-associated factor (TRAF)-like family protein SEVEN IN ABSENTIA 2 (SINA2) promotes drought tolerance in an ABA-dependent manner in Arabidopsis. New Phytol., 2014. 202(1): p. 174-87 [PMID:24350984] Yang YZ,Tan BC A distal ABA responsive element in AtNCED3 promoter is required for positive feedback regulation of ABA biosynthesis in Arabidopsis. PLoS ONE, 2014. 9(1): p. e87283 [PMID:24475264] González-Grandío E,Cubas P Identification of gene functions associated to active and dormant buds in Arabidopsis. Plant Signal Behav, 2014. 9(2): p. e27994 [PMID:24518068] Xie M, et al. AtWNK9 is regulated by ABA and dehydration and is involved in drought tolerance in Arabidopsis. Plant Physiol. Biochem., 2014. 77: p. 73-83 [PMID:24561249] Lumba S, et al. A mesoscale abscisic acid hormone interactome reveals a dynamic signaling landscape in Arabidopsis. Dev. Cell, 2014. 29(3): p. 360-72 [PMID:24823379] Kim EY,Seo YS,Park KY,Kim SJ,Kim WT Overexpression of CaDSR6 increases tolerance to drought and salt stresses in transgenic Arabidopsis plants. Gene, 2014. 552(1): p. 146-54 [PMID:25234727] Pruthvi V,Narasimhan R,Nataraja KN Simultaneous expression of abiotic stress responsive transcription factors, AtDREB2A, AtHB7 and AtABF3 improves salinity and drought tolerance in peanut (Arachis hypogaea L.). PLoS ONE, 2014. 9(12): p. e111152 [PMID:25474740] Zhang H, et al. The RING finger ubiquitin E3 ligase SDIR1 targets SDIR1-INTERACTING PROTEIN1 for degradation to modulate the salt stress response and ABA signaling in Arabidopsis. Plant Cell, 2015. 27(1): p. 214-27 [PMID:25616872] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178] Baek D, et al. A Role for Arabidopsis miR399f in Salt, Drought, and ABA Signaling. Mol. Cells, 2016. 39(2): p. 111-8 [PMID:26674968] Bao Y, et al. Overexpression of the NDR1/HIN1-Like Gene NHL6 Modifies Seed Germination in Response to Abscisic Acid and Abiotic Stresses in Arabidopsis. PLoS ONE, 2016. 11(2): p. e0148572 [PMID:26849212] Gao S, et al. ABF2, ABF3, and ABF4 Promote ABA-Mediated Chlorophyll Degradation and Leaf Senescence by Transcriptional Activation of Chlorophyll Catabolic Genes and Senescence-Associated Genes in Arabidopsis. Mol Plant, 2016. 9(9): p. 1272-1285 [PMID:27373216] Li X, et al. Dual Function of NAC072 in ABF3-Mediated ABA-Responsive Gene Regulation in Arabidopsis. Front Plant Sci, 2016. 7: p. 1075 [PMID:27486475] Wang Z, et al. Overexpressing Arabidopsis ABF3 increases tolerance to multiple abiotic stresses and reduces leaf size in alfalfa. Plant Physiol. Biochem., 2016. 109: p. 199-208 [PMID:27721135] Song L, et al. A transcription factor hierarchy defines an environmental stress response network. Science, 2017. [PMID:27811239] Kerr TCC, et al. Ectopic expression of two AREB/ABF orthologs increases drought tolerance in cotton (Gossypium hirsutum). Plant Cell Environ., 2018. 41(5): p. 898-907 [PMID:28098349] Lyzenga WJ,Sullivan V,Liu H,Stone SL The Kinase Activity of Calcineurin B-like Interacting Protein Kinase 26 (CIPK26) Influences Its Own Stability and that of the ABA-regulated Ubiquitin Ligase, Keep on Going (KEG). Front Plant Sci, 2017. 8: p. 502 [PMID:28443108] Song C,Kim T,Chung WS,Lim CO The Arabidopsis Phytocystatin AtCYS5 Enhances Seed Germination and Seedling Growth under Heat Stress Conditions. Mol. Cells, 2017. 40(8): p. 577-586 [PMID:28756655] Fernando VCD,Al Khateeb W,Belmonte MF,Schroeder DF Role of Arabidopsis ABF1/3/4 during det1 germination in salt and osmotic stress conditions. Plant Mol. Biol., 2018. 97(1-2): p. 149-163 [PMID:29680877] Kim HJ, et al. Confirmation of Drought Tolerance of Ectopically Expressed AtABF3 Gene in Soybean. Mol. Cells, 2018. 41(5): p. 413-422 [PMID:29754472]