PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT4G31920.1

Common Name

ARP4, ARR10, F10N7.270, RR10

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

ARR-B

Protein Properties

Length: 552aa MW: 61652.2 Da PI: 6.2321

Description

response regulator 10

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT4G31920.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	G2-like	83.4	2.5e-26	183	236	1	55
G2-like 1 kprlrWtpeLHerFveaveqLGGsekAtPktilelmkvkgLtlehvkSHLQkYRl 55 kpr+ Wt+eLH++F+ av++L G e+A+Pk+il+lm+v+ Lt+e+v+SHLQk+R+ AT4G31920.1 183 KPRVLWTHELHNKFLAAVDHL-GVERAVPKKILDLMNVDKLTRENVASHLQKFRV 236 79*****************.******************************7 PP
2	Response_reg	88	2.7e-29	19	127	1	109
EEEESSSHHHHHHHHHHHHHTTCEEEEEESSHHHHHHHHHHHH..ESEEEEESSCTTSEHHHHHHHHHHHTTTSEEEEEESTTTHHHHHHHHHTTES CS Response_reg 1 vlivdDeplvrellrqalekegyeevaeaddgeealellkekd..pDlillDiempgmdGlellkeireeepklpiivvtahgeeedalealkaGak 95 vl vdD+++ +++l+++l++ +y +v+++ +++ alell+e++ +Dl++ D++mp+mdG++ll+ e++lp+i+++ah++++++ + +k Ga AT4G31920.1 19 VLAVDDDQTCLRILQTLLQRCQY-HVTTTNQAQTALELLRENKnkFDLVISDVDMPDMDGFKLLELVGL-EMDLPVIMLSAHSDPKYVMKGVKHGAC 113 799******************.***********888888***************87754.558******************** PP EEEESS--HHHHHH CS Response_reg 96 dflsKpfdpeelvk 109 d+l Kp+ +eel + AT4G31920.1 114 DYLLKPVRIEELKN 127 *********987 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PIRSF	PIRSF036392	3.4E-167	1	541	IPR017053	Response regulator B-type, plant
SuperFamily	SSF52172	9.11E-37	16	141	IPR011006	CheY-like superfamily
Gene3D	G3DSA:3.40.50.2300	2.4E-44	16	166	No hit	No description
SMART	SM00448	1.1E-34	17	129	IPR001789	Signal transduction response regulator, receiver domain
PROSITE profile	PS50110	43.905	18	133	IPR001789	Signal transduction response regulator, receiver domain
Pfam	PF00072	2.7E-26	19	127	IPR001789	Signal transduction response regulator, receiver domain
CDD	cd00156	6.44E-30	20	132	No hit	No description
PROSITE profile	PS51294	13.742	180	239	IPR017930	Myb domain
Gene3D	G3DSA:1.10.10.60	4.7E-27	180	240	IPR009057	Homeodomain-like
SuperFamily	SSF46689	5.91E-19	181	240	IPR009057	Homeodomain-like
TIGRFAMs	TIGR01557	1.6E-24	183	236	IPR006447	Myb domain, plants
Pfam	PF00249	1.4E-5	187	234	IPR001005	SANT/Myb domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0000160	Biological Process	phosphorelay signal transduction system
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009414	Biological Process	response to water deprivation
GO:0010082	Biological Process	regulation of root meristem growth
GO:0010380	Biological Process	regulation of chlorophyll biosynthetic process
GO:0031537	Biological Process	regulation of anthocyanin metabolic process
GO:0048367	Biological Process	shoot system development
GO:0080022	Biological Process	primary root development
GO:0080036	Biological Process	regulation of cytokinin-activated signaling pathway
GO:0080113	Biological Process	regulation of seed growth
GO:0005634	Cellular Component	nucleus
GO:0000156	Molecular Function	phosphorelay response regulator activity
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000025	anatomy	root tip
PO:0000036	anatomy	leaf vascular system
PO:0000037	anatomy	shoot apex
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0005660	anatomy	hydathode
PO:0006079	anatomy	shoot system meristem
PO:0006339	anatomy	juvenile vascular leaf
PO:0008019	anatomy	leaf lamina base
PO:0009005	anatomy	root
PO:0009006	anatomy	shoot system
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0025281	anatomy	pollen
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007064	developmental stage	LP.12 twelve leaves visible stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 552 aa Download sequence Send to blast
MTMEQEIEVL DQFPVGMRVL AVDDDQTCLR ILQTLLQRCQ YHVTTTNQAQ TALELLRENK 60 NKFDLVISDV DMPDMDGFKL LELVGLEMDL PVIMLSAHSD PKYVMKGVKH GACDYLLKPV 120 RIEELKNIWQ HVVRKSKLKK NKSNVSNGSG NCDKANRKRK EQYEEEEEEE RGNDNDDPTA 180 QKKPRVLWTH ELHNKFLAAV DHLGVERAVP KKILDLMNVD KLTRENVASH LQKFRVALKK 240 VSDDAIQQAN RAAIDSHFMQ MNSQKGLGGF YHHHRGIPVG SGQFHGGTTM MRHYSSNRNL 300 GRLNSLGAGM FQPVSSSFPR NHNDGGNILQ GLPLEELQIN NNINRAFPSF TSQQNSPMVA 360 PSNLLLLEGN PQSSSLPSNP GFSPHFEISK RLEHWSNAAL STNIPQSDVH SKPDTLEWNA 420 FCDSASPLVN PNLDTNPASL CRNTGFGSTN AAQTDFFYPL QMNQQPANNS GPVTEAQLFR 480 SSNPNEGLLM GQQKLQSGLM ASDAGSLDDI VNSLMTQEQS QSDFSEGDWD LDGLAHSEHA 540 YEKLHFPFSL SA

Sequence ? help Back to Top

Protein Sequence Length: 552 aa Download sequence Send to blast

MTMEQEIEVL DQFPVGMRVL AVDDDQTCLR ILQTLLQRCQ YHVTTTNQAQ TALELLRENK  60
NKFDLVISDV DMPDMDGFKL LELVGLEMDL PVIMLSAHSD PKYVMKGVKH GACDYLLKPV  120
RIEELKNIWQ HVVRKSKLKK NKSNVSNGSG NCDKANRKRK EQYEEEEEEE RGNDNDDPTA  180
QKKPRVLWTH ELHNKFLAAV DHLGVERAVP KKILDLMNVD KLTRENVASH LQKFRVALKK  240
VSDDAIQQAN RAAIDSHFMQ MNSQKGLGGF YHHHRGIPVG SGQFHGGTTM MRHYSSNRNL  300
GRLNSLGAGM FQPVSSSFPR NHNDGGNILQ GLPLEELQIN NNINRAFPSF TSQQNSPMVA  360
PSNLLLLEGN PQSSSLPSNP GFSPHFEISK RLEHWSNAAL STNIPQSDVH SKPDTLEWNA  420
FCDSASPLVN PNLDTNPASL CRNTGFGSTN AAQTDFFYPL QMNQQPANNS GPVTEAQLFR  480
SSNPNEGLLM GQQKLQSGLM ASDAGSLDDI VNSLMTQEQS QSDFSEGDWD LDGLAHSEHA  540
YEKLHFPFSL SA

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1irz_A	7e-36	179	242	1	64	ARR10-B
Search in ModeBase

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.122	0.0	flower\| leaf\| root\| seed

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	145350987	0.0
Genevisible	253500_at	0.0
Expression Atlas	AT4G31920	-
AtGenExpress	AT4G31920	-
ATTED-II	AT4G31920	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Detected in the whole plant. Predominantly expressed in roots and leaves. {ECO:0000269\|PubMed:15173562}.

Functional Description ? help Back to Top
Source	Description
TAIR	Encodes an Arabidopsis response regulator (ARR) protein that acts in concert with other type-B ARRs in the cytokinin signaling pathway.
UniProt	Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]GATT-3'. Functions as a response regulator involved in His-to-Asp phosphorelay signal transduction system. Phosphorylation of the Asp residue in the receiver domain activates the ability of the protein to promote the transcription of target genes. Could directly activate some type-A response regulators in response to cytokinins. {ECO:0000269\|PubMed:11574878}.

Function -- GeneRIF ? help Back to Top
ARR10 and ARR12play pivotal roles in the histidine kinase-dependent phosphorelay signaling in response to cytokinin in roots. [PMID: 17132632] The results of this study suggested that ARR1, ARR10 and ARR12 together play essential (or general) roles in cytokinin signal transduction. [PMID: 18037673] Results support a model in which cytokinin regulates a wide array of downstream responses through the action of a multistep phosphorelay that culminates in transcriptional regulation by ARR1, ARR10, and ARR12. [PMID: 18723577] An arr1,10,12 triple mutant had more drought tolerance due to enhanced cell membrane integrity, anthocyanin synthesis, and abscissic acid hypersensitivity, and reduced stomatal aperture. Dehydration and ABA repressed all 3 genes. [PMID: 26884175] The role of ARR10 in regulating the cytokinin response and growth in Arabidopsis [PMID: 28673986] The expression signals of AG were detected in the initiating carpel primordia and regenerating carpels, and co-localized with those of two Type-B ARABIDOPSIS RESPONSE REGULATORs (ARRs), ARR1 and ARR10. [PMID: 29186581]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00072	SELEX	26531826	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT4G31920.1

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source	Target Gene (A: Activate/R: Repress)
ATRM	AT5G62920(A)

Regulation -- Hormone ? help Back to Top
Source	Hormone
AHD	cytokinin

Interaction ? help Back to Top
Source	Intact With
IntAct	Search O49397

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT4G31920

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AJ005195	0.0	AJ005195.1 Arabidopsis thaliana mRNA for receiver-like protein 4.
GenBank	AK228149	0.0	AK228149.1 Arabidopsis thaliana mRNA for predicted protein, complete cds, clone: RAFL14-61-J20.
GenBank	BT003158	0.0	BT003158.1 Arabidopsis thaliana At4g31920 mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_194920.1	0.0	response regulator 10
Swissprot	O49397	0.0	ARR10_ARATH; Two-component response regulator ARR10
TrEMBL	A0A384KXN7	0.0	A0A384KXN7_ARATH; Two-component response regulator
TrEMBL	Q0WRZ4	0.0	Q0WRZ4_ARATH; Two-component response regulator
STRING	AT4G31920.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM1981	28	79
Representative plant	OGRP292	17	120

Link Out ? help Back to Top
Phytozome	AT4G31920.1
Entrez Gene	829322
iHOP	AT4G31920
wikigenes	AT4G31920

Publications ? help Back to Top
Imamura A, et al. Compilation and characterization of Arabidopsis thaliana response regulators implicated in His-Asp phosphorelay signal transduction. Plant Cell Physiol., 1999. 40(7): p. 733-42 [PMID:10501033] Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Suzuki T,Sakurai K,Ueguchi C,Mizuno T Two types of putative nuclear factors that physically interact with histidine-containing phosphotransfer (Hpt) domains, signaling mediators in His-to-Asp phosphorelay, in Arabidopsis thaliana. Plant Cell Physiol., 2001. 42(1): p. 37-45 [PMID:11158442] Hwang I,Sheen J Two-component circuitry in Arabidopsis cytokinin signal transduction. Nature, 2001. 413(6854): p. 383-9 [PMID:11574878] Haberer G,Kieber JJ Cytokinins. New insights into a classic phytohormone. Plant Physiol., 2002. 128(2): p. 354-62 [PMID:11842139] Hwang I,Chen HC,Sheen J Two-component signal transduction pathways in Arabidopsis. Plant Physiol., 2002. 129(2): p. 500-15 [PMID:12068096] Hosoda K, et al. Molecular structure of the GARP family of plant Myb-related DNA binding motifs of the Arabidopsis response regulators. Plant Cell, 2002. 14(9): p. 2015-29 [PMID:12215502] Che P,Gingerich DJ,Lall S,Howell SH Global and hormone-induced gene expression changes during shoot development in Arabidopsis. Plant Cell, 2002. 14(11): p. 2771-85 [PMID:12417700] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Mason MG,Li J,Mathews DE,Kieber JJ,Schaller GE Type-B response regulators display overlapping expression patterns in Arabidopsis. Plant Physiol., 2004. 135(2): p. 927-37 [PMID:15173562] Mason MG, et al. Multiple type-B response regulators mediate cytokinin signal transduction in Arabidopsis. Plant Cell, 2005. 17(11): p. 3007-18 [PMID:16227453] Dortay H,Mehnert N,B Analysis of protein interactions within the cytokinin-signaling pathway of Arabidopsis thaliana. FEBS J., 2006. 273(20): p. 4631-44 [PMID:16965536] Yokoyama A, et al. Type-B ARR transcription factors, ARR10 and ARR12, are implicated in cytokinin-mediated regulation of protoxylem differentiation in roots of Arabidopsis thaliana. Plant Cell Physiol., 2007. 48(1): p. 84-96 [PMID:17132632] Ishida K,Yamashino T,Yokoyama A,Mizuno T Three type-B response regulators, ARR1, ARR10 and ARR12, play essential but redundant roles in cytokinin signal transduction throughout the life cycle of Arabidopsis thaliana. Plant Cell Physiol., 2008. 49(1): p. 47-57 [PMID:18037673] Dortay H, et al. Toward an interaction map of the two-component signaling pathway of Arabidopsis thaliana. J. Proteome Res., 2008. 7(9): p. 3649-60 [PMID:18642946] Ascencio-Ib Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection. Plant Physiol., 2008. 148(1): p. 436-54 [PMID:18650403] Argyros RD, et al. Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development. Plant Cell, 2008. 20(8): p. 2102-16 [PMID:18723577] Perochon A, et al. Interaction of a plant pseudo-response regulator with a calmodulin-like protein. Biochem. Biophys. Res. Commun., 2010. 398(4): p. 747-51 [PMID:20627089] Kondo Y,Hirakawa Y,Kieber JJ,Fukuda H CLE peptides can negatively regulate protoxylem vessel formation via cytokinin signaling. Plant Cell Physiol., 2011. 52(1): p. 37-48 [PMID:20802224] Nemoto K, et al. Autophosphorylation profiling of Arabidopsis protein kinases using the cell-free system. Phytochemistry, 2011. 72(10): p. 1136-44 [PMID:21477822] Kushwah S,Jones AM,Laxmi A Cytokinin interplay with ethylene, auxin, and glucose signaling controls Arabidopsis seedling root directional growth. Plant Physiol., 2011. 156(4): p. 1851-66 [PMID:21666052] Cutcliffe JW,Hellmann E,Heyl A,Rashotte AM CRFs form protein-protein interactions with each other and with members of the cytokinin signalling pathway in Arabidopsis via the CRF domain. J. Exp. Bot., 2011. 62(14): p. 4995-5002 [PMID:21705390] Das PK, et al. Cytokinins enhance sugar-induced anthocyanin biosynthesis in Arabidopsis. Mol. Cells, 2012. 34(1): p. 93-101 [PMID:22699753] Efroni I, et al. Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses. Dev. Cell, 2013. 24(4): p. 438-45 [PMID:23449474] Hill K, et al. Functional characterization of type-B response regulators in the Arabidopsis cytokinin response. Plant Physiol., 2013. 162(1): p. 212-24 [PMID:23482873] Hill K,Schaller GE Enhancing plant regeneration in tissue culture: a molecular approach through manipulation of cytokinin sensitivity. Plant Signal Behav, 2013. 8(10): p. doi: 10.4161/psb.25709 [PMID:23887495] Ramireddy E,Chang L,Schmülling T Cytokinin as a mediator for regulating root system architecture in response to environmental cues. Plant Signal Behav, 2014. 9(1): p. e27771 [PMID:24509549] Choi SH, et al. Gene duplication of type-B ARR transcription factors systematically extends transcriptional regulatory structures in Arabidopsis. Sci Rep, 2014. 4: p. 7197 [PMID:25425016] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178] Nguyen KH, et al. Arabidopsis type B cytokinin response regulators ARR1, ARR10, and ARR12 negatively regulate plant responses to drought. Proc. Natl. Acad. Sci. U.S.A., 2016. 113(11): p. 3090-5 [PMID:26884175] Cortleven A, et al. Cytokinin Regulates the Etioplast-Chloroplast Transition through the Two-Component Signaling System and Activation of Chloroplast-Related Genes. Plant Physiol., 2016. 172(1): p. 464-78 [PMID:27388681] Street IH, et al. Cytokinin acts through the auxin influx carrier AUX1 to regulate cell elongation in the root. Development, 2016. 143(21): p. 3982-3993 [PMID:27697901] Wang Y,Wu Y,Yu B,Yin Z,Xia Y EXTRA-LARGE G PROTEINs Interact with E3 Ligases PUB4 and PUB2 and Function in Cytokinin and Developmental Processes. Plant Physiol., 2017. 173(2): p. 1235-1246 [PMID:27986866] Zhang TQ, et al. A Two-Step Model for de Novo Activation of WUSCHEL during Plant Shoot Regeneration. Plant Cell, 2017. 29(5): p. 1073-1087 [PMID:28389585] Meng WJ, et al. Type-B ARABIDOPSIS RESPONSE REGULATORs Specify the Shoot Stem Cell Niche by Dual Regulation of WUSCHEL. Plant Cell, 2017. 29(6): p. 1357-1372 [PMID:28576846] Zubo YO, et al. Cytokinin induces genome-wide binding of the type-B response regulator ARR10 to regulate growth and development in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A., 2017. 114(29): p. E5995-E6004 [PMID:28673986] Zhang F,May A,Irish VF Type-B ARABIDOPSIS RESPONSE REGULATORs Directly Activate WUSCHEL. Trends Plant Sci., 2017. 22(10): p. 815-817 [PMID:28886911] Rong XF, et al. Type-B ARRs Control Carpel Regeneration Through Mediating AGAMOUS Expression in Arabidopsis. Plant Cell Physiol., 2018. 59(4): p. 756-764 [PMID:29186581] Bustillo-Avendaño E, et al. Regulation of Hormonal Control, Cell Reprogramming, and Patterning during De Novo Root Organogenesis. Plant Physiol., 2018. 176(2): p. 1709-1727 [PMID:29233938]