PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT4G31800.1

Common Name

ATWRKY18, F28M20.10, WRKY18

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

WRKY

Protein Properties

Length: 310aa MW: 34765.8 Da PI: 7.3768

Description

WRKY DNA-binding protein 18

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT4G31800.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	93.5	1.5e-29	175	233	1	58
---SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS CS WRKY 1 ldDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnh 58 ++Dg++WrKYGqK+ ++++ pr+Y+rC++a +Cpvkkkv+rsaedp++++ tYeg+Hnh AT4G31800.1 175 VKDGFQWRKYGQKVTRDNPSPRAYFRCSFApSCPVKKKVQRSAEDPSLLVATYEGTHNH 233 58*************************99************************** PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	2.4E-28	165	234	IPR003657	WRKY domain
SuperFamily	SSF118290	8.63E-25	171	234	IPR003657	WRKY domain
SMART	SM00774	1.4E-33	175	235	IPR003657	WRKY domain
Pfam	PF03106	5.3E-24	176	233	IPR003657	WRKY domain
PROSITE profile	PS50811	25.499	177	236	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0002237	Biological Process	response to molecule of bacterial origin
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009751	Biological Process	response to salicylic acid
GO:0010200	Biological Process	response to chitin
GO:0042742	Biological Process	defense response to bacterium
GO:0050691	Biological Process	regulation of defense response to virus by host
GO:0050832	Biological Process	defense response to fungus
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0042802	Molecular Function	identical protein binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000005	anatomy	cultured plant cell
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0005417	anatomy	phloem
PO:0008019	anatomy	leaf lamina base
PO:0009005	anatomy	root
PO:0009006	anatomy	shoot system
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0025034	anatomy	leaf
PO:0025281	anatomy	pollen
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007064	developmental stage	LP.12 twelve leaves visible stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 310 aa Download sequence Send to blast
MDGSSFLDIS LDLNTNPFSA KLPKKEVSVL ASTHLKRKWL EQDESASELR EELNRVNSEN 60 KKLTEMLARV CESYNELHNH LEKLQSRQSP EIEQTDIPIK KRKQDPDEFL GFPIGLSSGK 120 TENSSSNEDH HHHHQQHEQK NQLLSCKRPV TDSFNKAKVS TVYVPTETSD TSLTVKDGFQ 180 WRKYGQKVTR DNPSPRAYFR CSFAPSCPVK KKVQRSAEDP SLLVATYEGT HNHLGPNASE 240 GDATSQGGSS TVTLDLVNGC HRLALEKNER DNTMQEVLIQ QMASSLTKDS KFTAALAAAI 300 SGRLMEQSRT

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wj2_A	1e-19	168	233	11	74	Probable WRKY transcription factor 4
2lex_A	1e-19	168	233	11	74	Probable WRKY transcription factor 4
Search in ModeBase

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.19031	0.0	flower\| leaf\| root\| seed

Expression -- Microarray ? help Back to Top
Source	ID	E-value
Genevisible	253485_at	0.0
Expression Atlas	AT4G31800	-
AtGenExpress	AT4G31800	-
ATTED-II	AT4G31800	-

Functional Description ? help Back to Top
Source	Description
TAIR	Pathogen-induced transcription factor. Binds W-box sequences in vitro. Forms protein complexes with itself and with WRKY40 and WRKY60. Constitutive expression of WRKY18 enhanced resistance to P. syringae, but its coexpression with WRKY40 or WRKY60 made plants more susceptible to both P. syringae and B. cinerea. WRKY18, WRKY40, and WRKY60 have partially redundant roles in response to the hemibiotrophic bacterial pathogen Pseudomonas syringae and the necrotrophic fungal pathogen Botrytis cinerea, with WRKY18 playing a more important role than the other two.
UniProt	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Positively modulates defense-related gene expression and disease resistance. {ECO:0000269\|PubMed:12068113}.

Function -- GeneRIF ? help Back to Top
WRKY18 interacts both physically and functionally with WRKY40 and WRKY60 in a complex pattern of overlapping, antagonistic, and distinct roles in plant responses to different types of microbial pathogens. [PMID: 16603654] Loss-of-WRKY18/40 positively affects pre-invasion resistance to G. orontii. [PMID: 21143673] Data suggest that WRKY18, WRKY40 and WRKY60 form a highly interacting regulatory network that modulates gene expression in both plant defense and stress responses by acting as either transcription activator or repressor. [PMID: 21167067] The susceptibility of wrky18 mutant lines to volatiles was significantly delayed, suggesting an indispensable role for WRKY18 in bacterial volatile responses. [PMID: 22188129] Comprehensive infection studies demonstrated the specificity of wrky18 wrky40-mediated Golovinomyces orontii resistance. WRKY18 and WRKY40 act as positive regulators in effector-triggered immunity. [PMID: 23617415] Binding of WRKY18, WRKY40, and WRKY33 to promoters of genes Implicated in microbe-associated molecular patterns (MAMPs)-triggered Immunity. [PMID: 28011690]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00465	DAP	27203113	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT4G31800.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By salicylic acid and pathogens. {ECO:0000269\|PubMed:11449049, ECO:0000269\|PubMed:12068113}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source	Target Gene (A: Activate/R: Repress)
ATRM	AT1G64280(A), AT1G75040(A), AT2G14610(A), AT4G00340(A)

Regulation -- Hormone ? help Back to Top
Source	Hormone
AHD	salicylic acid

Interaction ? help Back to Top
Source	Intact With
BioGRID	AT4G31800, AT1G80840
IntAct	Search Q9C5T4

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT4G31800

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AF428421	0.0	AF428421.1 Arabidopsis thaliana AT4g31800/F28M20_10 mRNA, complete cds.
GenBank	AK227401	0.0	AK227401.1 Arabidopsis thaliana mRNA for WRKY like transcription factor, complete cds, clone: RAFL14-06-B12.
GenBank	AY125557	0.0	AY125557.1 Arabidopsis thaliana AT4g31800/F28M20_10 mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_567882.1	0.0	WRKY DNA-binding protein 18
Swissprot	Q9C5T4	0.0	WRK18_ARATH; WRKY transcription factor 18
TrEMBL	Q0WTZ3	0.0	Q0WTZ3_ARATH; WRKY like transcription factor
STRING	AT4G31800.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM6584	19	45
Representative plant	OGRP14	17	875

Link Out ? help Back to Top
Phytozome	AT4G31800.1
Entrez Gene	829308
iHOP	AT4G31800
wikigenes	AT4G31800

Publications ? help Back to Top
Eulgem T,Rushton PJ,Robatzek S,Somssich IE The WRKY superfamily of plant transcription factors. Trends Plant Sci., 2000. 5(5): p. 199-206 [PMID:10785665] Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Yu D,Chen C,Chen Z Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression. Plant Cell, 2001. 13(7): p. 1527-40 [PMID:11449049] Cheong YH, et al. Transcriptional profiling reveals novel interactions between wounding, pathogen, abiotic stress, and hormonal responses in Arabidopsis. Plant Physiol., 2002. 129(2): p. 661-77 [PMID:12068110] Chen C,Chen Z Potentiation of developmentally regulated plant defense response by AtWRKY18, a pathogen-induced Arabidopsis transcription factor. Plant Physiol., 2002. 129(2): p. 706-16 [PMID:12068113] Dong J,Chen C,Chen Z Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response. Plant Mol. Biol., 2003. 51(1): p. 21-37 [PMID:12602888] Keates SE,Kostman TA,Anderson JD,Bailey BA Altered gene expression in three plant species in response to treatment with Nep1, a fungal protein that causes necrosis. Plant Physiol., 2003. 132(3): p. 1610-22 [PMID:12857840] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Xu X,Chen C,Fan B,Chen Z Physical and functional interactions between pathogen-induced Arabidopsis WRKY18, WRKY40, and WRKY60 transcription factors. Plant Cell, 2006. 18(5): p. 1310-26 [PMID:16603654] Alignan M,Hewezi T,Petitprez M,Dechamp-Guillaume G,Gentzbittel L A cDNA microarray approach to decipher sunflower (Helianthus annuus) responses to the necrotrophic fungus Phoma macdonaldii. New Phytol., 2006. 170(3): p. 523-36 [PMID:16626474] Bülow L,Steffens NO,Galuschka C,Schindler M,Hehl R AthaMap: from in silico data to real transcription factor binding sites. In Silico Biol. (Gedrukt), 2006. 6(3): p. 243-52 [PMID:16922688] Kim KC,Fan B,Chen Z Pathogen-induced Arabidopsis WRKY7 is a transcriptional repressor and enhances plant susceptibility to Pseudomonas syringae. Plant Physiol., 2006. 142(3): p. 1180-92 [PMID:16963526] Wang D,Amornsiripanitch N,Dong X A genomic approach to identify regulatory nodes in the transcriptional network of systemic acquired resistance in plants. PLoS Pathog., 2006. 2(11): p. e123 [PMID:17096590] Shen QH, et al. Nuclear activity of MLA immune receptors links isolate-specific and basal disease-resistance responses. Science, 2007. 315(5815): p. 1098-103 [PMID:17185563] Libault M,Wan J,Czechowski T,Udvardi M,Stacey G Identification of 118 Arabidopsis transcription factor and 30 ubiquitin-ligase genes responding to chitin, a plant-defense elicitor. Mol. Plant Microbe Interact., 2007. 20(8): p. 900-11 [PMID:17722694] Wang Z, et al. Identification and characterization of COI1-dependent transcription factor genes involved in JA-mediated response to wounding in Arabidopsis plants. Plant Cell Rep., 2008. 27(1): p. 125-35 [PMID:17786451] Deeken R, et al. Identification of Arabidopsis thaliana phloem RNAs provides a search criterion for phloem-based transcripts hidden in complex datasets of microarray experiments. Plant J., 2008. 55(5): p. 746-59 [PMID:18485061] Ascencio-Ib Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection. Plant Physiol., 2008. 148(1): p. 436-54 [PMID:18650403] Kim KC,Lai Z,Fan B,Chen Z Arabidopsis WRKY38 and WRKY62 transcription factors interact with histone deacetylase 19 in basal defense. Plant Cell, 2008. 20(9): p. 2357-71 [PMID:18776063] Chandran D, et al. Temporal global expression data reveal known and novel salicylate-impacted processes and regulators mediating powdery mildew growth and reproduction on Arabidopsis. Plant Physiol., 2009. 149(3): p. 1435-51 [PMID:19176722] Chang IF, et al. Proteomic profiling of tandem affinity purified 14-3-3 protein complexes in Arabidopsis thaliana. Proteomics, 2009. 9(11): p. 2967-85 [PMID:19452453] Peng Y, et al. OsWRKY62 is a negative regulator of basal and Xa21-mediated defense against Xanthomonas oryzae pv. oryzae in rice. Mol Plant, 2008. 1(3): p. 446-58 [PMID:19825552] Makandar R, et al. Involvement of salicylate and jasmonate signaling pathways in Arabidopsis interaction with Fusarium graminearum. Mol. Plant Microbe Interact., 2010. 23(7): p. 861-70 [PMID:20521949] Shang Y, et al. The Mg-chelatase H subunit of Arabidopsis antagonizes a group of WRKY transcription repressors to relieve ABA-responsive genes of inhibition. Plant Cell, 2010. 22(6): p. 1909-35 [PMID:20543028] Pandey SP,Roccaro M,Sch Transcriptional reprogramming regulated by WRKY18 and WRKY40 facilitates powdery mildew infection of Arabidopsis. Plant J., 2010. 64(6): p. 912-23 [PMID:21143673] Chen H, et al. Roles of arabidopsis WRKY18, WRKY40 and WRKY60 transcription factors in plant responses to abscisic acid and abiotic stress. BMC Plant Biol., 2010. 10: p. 281 [PMID:21167067] Arabidopsis Interactome Mapping Consortium Evidence for network evolution in an Arabidopsis interactome map. Science, 2011. 333(6042): p. 601-7 [PMID:21798944] Wenke K, et al. Volatiles of two growth-inhibiting rhizobacteria commonly engage AtWRKY18 function. Plant J., 2012. 70(3): p. 445-59 [PMID:22188129] Liu ZQ, et al. Cooperation of three WRKY-domain transcription factors WRKY18, WRKY40, and WRKY60 in repressing two ABA-responsive genes ABI4 and ABI5 in Arabidopsis. J. Exp. Bot., 2012. 63(18): p. 6371-92 [PMID:23095997] Schweizer F,Bodenhausen N,Lassueur S,Masclaux FG,Reymond P Differential Contribution of Transcription Factors to Arabidopsis thaliana Defense Against Spodoptera littoralis. Front Plant Sci, 2013. 4: p. 13 [PMID:23382734] Brotman Y, et al. Trichoderma-plant root colonization: escaping early plant defense responses and activation of the antioxidant machinery for saline stress tolerance. PLoS Pathog., 2013. 9(3): p. e1003221 [PMID:23516362] Sch Analyses of wrky18 wrky40 plants reveal critical roles of SA/EDS1 signaling and indole-glucosinolate biosynthesis for Golovinomyces orontii resistance and a loss-of resistance towards Pseudomonas syringae pv. tomato AvrRPS4. Mol. Plant Microbe Interact., 2013. 26(7): p. 758-67 [PMID:23617415] Koyama T, et al. A regulatory cascade involving class II ETHYLENE RESPONSE FACTOR transcriptional repressors operates in the progression of leaf senescence. Plant Physiol., 2013. 162(2): p. 991-1005 [PMID:23629833] Zhang XF, et al. Cochaperonin CPN20 negatively regulates abscisic acid signaling in Arabidopsis. Plant Mol. Biol., 2013. 83(3): p. 205-18 [PMID:23783410] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Zhang Y, et al. Regulation of oncogene expression in T-DNA-transformed host plant cells. PLoS Pathog., 2015. 11(1): p. e1004620 [PMID:25615824] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178] Geilen K,Böhmer M Dynamic subnuclear relocalisation of WRKY40 in response to Abscisic acid in Arabidopsis thaliana. Sci Rep, 2015. 5: p. 13369 [PMID:26293691] Geilen K,Böhmer M Dynamic subnuclear relocalization of WRKY40, a potential new mechanism of ABA-dependent transcription factor regulation. Plant Signal Behav, 2015. 10(11): p. e1106659 [PMID:26479147] Lu K, et al. Overexpression of an Arabidopsis cysteine-rich receptor-like protein kinase, CRK5, enhances abscisic acid sensitivity and confers drought tolerance. J. Exp. Bot., 2016. 67(17): p. 5009-27 [PMID:27406784] Birkenbihl RP,Kracher B,Roccaro M,Somssich IE Induced Genome-Wide Binding of Three Arabidopsis WRKY Transcription Factors during Early MAMP-Triggered Immunity. Plant Cell, 2017. 29(1): p. 20-38 [PMID:28011690]