PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT4G27950.1
Common NameCRF4, ERF066, T13J8.60
Organism
Arabidopsis thaliana
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family ERF
Protein Properties Length: 335aa    MW: 38447.6 Da    PI: 4.358
Description cytokinin response factor 4
Gene Model
Gene Model ID Type Source Coding Sequence
AT4G27950.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1AP265.31.2e-20116166155
          AP2   1 sgykGVrwdkkrgrWvAeIrdpsengkrkrfslgkfgtaeeAakaaiaarkkleg 55 
                  ++y+GVr+++ +g+W+AeIrdp +   r+r++lg+f taeeAa  +++a+ kl+g
  AT4G27950.1 116 KKYRGVRQRP-WGKWAAEIRDPEQ---RRRIWLGTFATAEEAAIVYDNAAIKLRG 166
                  59********.**********743...5*************************99 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
CDDcd000181.84E-34116176No hitNo description
SMARTSM003801.1E-41117180IPR001471AP2/ERF domain
Gene3DG3DSA:3.30.730.102.0E-30117174IPR001471AP2/ERF domain
PROSITE profilePS5103223.788117174IPR001471AP2/ERF domain
SuperFamilySSF541714.18E-22117175IPR016177DNA-binding domain
PfamPF008474.9E-13117166IPR001471AP2/ERF domain
PRINTSPR003671.9E-10118129IPR001471AP2/ERF domain
PRINTSPR003671.9E-10140156IPR001471AP2/ERF domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009736Biological Processcytokinin-activated signaling pathway
GO:0009873Biological Processethylene-activated signaling pathway
GO:0042991Biological Processtranscription factor import into nucleus
GO:0048366Biological Processleaf development
GO:0005634Cellular Componentnucleus
GO:0005737Cellular Componentcytoplasm
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000293anatomyguard cell
Sequence ? help Back to Top
Protein Sequence    Length: 335 aa     Download sequence    Send to blast
MMMDEFMDLR PVKYTEHKTV IRKYTKKSSM ERKTSVRDSA RLVRVSMTDR DATDSSSDEE  60
EFLFPRRRVK RLINEIRVEP SSSSTGDVSA SPTKDRKRIN VDSTVQKPSV SGQNQKKYRG  120
VRQRPWGKWA AEIRDPEQRR RIWLGTFATA EEAAIVYDNA AIKLRGPDAL TNFTVQPEPE  180
PVQEQEQEPE SNMSVSISES MDDSQHLSSP TSVLNYQTYV SEEPIDSLIK PVKQEFLEPE  240
QEPISWHLGE GNTNTNDDSF PLDITFLDNY FNESLPDISI FDQPMSPIQP TENDFFNDLM  300
LFDSNAEEYY SSEIKEIGSS FNDLDDSLIS DLLLV
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
1gcc_A1e-21116176162ETHYLENE RESPONSIVE ELEMENT BINDING FACTOR 1
2gcc_A2e-21116176465ATERF1
3gcc_A2e-21116176465ATERF1
Search in ModeBase
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO425671980.0
Expression AtlasAT4G27950-
AtGenExpressAT4G27950-
Functional Description ? help Back to Top
Source Description
TAIRencodes a member of the ERF (ethylene response factor) subfamily B-5 of ERF/AP2 transcription factor family. The protein contains one AP2 domain. There are 7 members in this subfamily.
UniProtComponent of the cytokinin signaling pathway involved in cotyledons, leaves, and embryos development. Probably acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). {ECO:0000250, ECO:0000269|PubMed:16832061}.
Function -- GeneRIF ? help Back to Top
  1. Data indicate that mutants lacking expression of cytokinin response factor 4 (CRF4) were more sensitive to freezing.
    [PMID: 26650835]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00456DAP27203113Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT4G27950.1
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Interaction ? help Back to Top
Source Intact With
BioGRIDAT4G27950, AT5G53290, AT1G22985, AT1G71130
IntActSearch Q9SUE3
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT4G27950
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAL0355240.0AL035524.1 Arabidopsis thaliana DNA chromosome 4, BAC clone T13J8 (ESSAII project).
GenBankAL1615720.0AL161572.2 Arabidopsis thaliana DNA chromosome 4, contig fragment No. 68.
GenBankBT0330880.0BT033088.1 Arabidopsis thaliana unknown protein (At4g27950) mRNA, complete cds.
GenBankCP0026870.0CP002687.1 Arabidopsis thaliana chromosome 4 sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_194524.20.0cytokinin response factor 4
SwissprotQ9SUE30.0CRF4_ARATH; Ethylene-responsive transcription factor CRF4
TrEMBLA0A178V2V50.0A0A178V2V5_ARATH; CRF4
STRINGAT4G27950.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM22292576
Representative plantOGRP6161718
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Nakano T,Suzuki K,Fujimura T,Shinshi H
    Genome-wide analysis of the ERF gene family in Arabidopsis and rice.
    Plant Physiol., 2006. 140(2): p. 411-32
    [PMID:16407444]
  3. Rashotte AM, et al.
    A subset of Arabidopsis AP2 transcription factors mediates cytokinin responses in concert with a two-component pathway.
    Proc. Natl. Acad. Sci. U.S.A., 2006. 103(29): p. 11081-5
    [PMID:16832061]
  4. Cutcliffe JW,Hellmann E,Heyl A,Rashotte AM
    CRFs form protein-protein interactions with each other and with members of the cytokinin signalling pathway in Arabidopsis via the CRF domain.
    J. Exp. Bot., 2011. 62(14): p. 4995-5002
    [PMID:21705390]
  5. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
    [PMID:24377444]
  6. Zwack PJ,Compton MA,Adams CI,Rashotte AM
    Cytokinin response factor 4 (CRF4) is induced by cold and involved in freezing tolerance.
    Plant Cell Rep., 2016. 35(3): p. 573-84
    [PMID:26650835]
  7. Raines T, et al.
    The cytokinin response factors modulate root and shoot growth and promote leaf senescence in Arabidopsis.
    Plant J., 2016. 85(1): p. 134-47
    [PMID:26662515]