PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT4G26150.1

Common Name

CGA1, F20B18.260, GATA22, GNL

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

GATA

Protein Properties

Length: 352aa MW: 39345.3 Da PI: 9.5931

Description

cytokinin-responsive gata factor 1

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT4G26150.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	GATA	60.2	2.6e-19	201	234	1	34
GATA 1 CsnCgttkTplWRrgpdgnktLCnaCGlyyrkkg 34 Cs C+ttkTplWR+gp+g+k+LCnaCG+++rk + AT4G26150.1 201 CSDCNTTKTPLWRSGPRGPKSLCNACGIRQRKAR 234 *******************************987 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SMART	SM00401	4.1E-20	195	247	IPR000679	Zinc finger, GATA-type
SuperFamily	SSF57716	1.95E-12	198	235	No hit	No description
Gene3D	G3DSA:3.30.50.10	2.8E-15	199	234	IPR013088	Zinc finger, NHR/GATA-type
CDD	cd00202	2.11E-13	200	230	No hit	No description
PROSITE profile	PS50114	12.44	201	231	IPR000679	Zinc finger, GATA-type
Pfam	PF00320	5.0E-17	201	234	IPR000679	Zinc finger, GATA-type
PROSITE pattern	PS00344	0	201	226	IPR000679	Zinc finger, GATA-type

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0007623	Biological Process	circadian rhythm
GO:0009416	Biological Process	response to light stimulus
GO:0009735	Biological Process	response to cytokinin
GO:0009740	Biological Process	gibberellic acid mediated signaling pathway
GO:0009910	Biological Process	negative regulation of flower development
GO:0010187	Biological Process	negative regulation of seed germination
GO:0010380	Biological Process	regulation of chlorophyll biosynthetic process
GO:0010468	Biological Process	regulation of gene expression
GO:0030154	Biological Process	cell differentiation
GO:0045944	Biological Process	positive regulation of transcription from RNA polymerase II promoter
GO:0005634	Cellular Component	nucleus
GO:0005667	Cellular Component	transcription factor complex
GO:0000977	Molecular Function	RNA polymerase II regulatory region sequence-specific DNA binding
GO:0001085	Molecular Function	RNA polymerase II transcription factor binding
GO:0001228	Molecular Function	transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
GO:0003682	Molecular Function	chromatin binding
GO:0008270	Molecular Function	zinc ion binding
GO:0044212	Molecular Function	transcription regulatory region DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000037	anatomy	shoot apex
PO:0000293	anatomy	guard cell
PO:0008019	anatomy	leaf lamina base
PO:0009006	anatomy	shoot system
PO:0009009	anatomy	plant embryo
PO:0009025	anatomy	vascular leaf
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0025022	anatomy	collective leaf structure
PO:0001185	developmental stage	plant embryo globular stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 352 aa Download sequence Send to blast
MGSNFHYTID LNEDQNHQPF FASLGSSLHH HLQQQQQQQQ HFHHQASSNP SSLMSPSLSY 60 FPFLINSRQD QVYVGYNNNT FHDVLDTHIS QPLETKNFVS DGGSSSSDQM VPKKETRLKL 120 TIKKKDNHQD QTDLPQSPIK DMTGTNSLKW ISSKVRLMKK KKAIITTSDS SKQHTNNDQS 180 SNLSNSERQN GYNNDCVIRI CSDCNTTKTP LWRSGPRGPK SLCNACGIRQ RKARRAAMAT 240 ATATAVSGVS PPVMKKKMQN KNKISNGVYK ILSPLPLKVN TCKRMITLEE TALAEDLETQ 300 SNSTMLSSSD NIYFDDLALL LSKSSAYQQV FPQDEKEAAI LLMALSHGMV HG

Sequence ? help Back to Top

Protein Sequence Length: 352 aa Download sequence Send to blast

MGSNFHYTID LNEDQNHQPF FASLGSSLHH HLQQQQQQQQ HFHHQASSNP SSLMSPSLSY  60
FPFLINSRQD QVYVGYNNNT FHDVLDTHIS QPLETKNFVS DGGSSSSDQM VPKKETRLKL  120
TIKKKDNHQD QTDLPQSPIK DMTGTNSLKW ISSKVRLMKK KKAIITTSDS SKQHTNNDQS  180
SNLSNSERQN GYNNDCVIRI CSDCNTTKTP LWRSGPRGPK SLCNACGIRQ RKARRAAMAT  240
ATATAVSGVS PPVMKKKMQN KNKISNGVYK ILSPLPLKVN TCKRMITLEE TALAEDLETQ  300
SNSTMLSSSD NIYFDDLALL LSKSSAYQQV FPQDEKEAAI LLMALSHGMV HG

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	30687127	0.0
Genevisible	254016_at	0.0
Expression Atlas	AT4G26150	-
AtGenExpress	AT4G26150	-
ATTED-II	AT4G26150	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	DEVELOPMENTAL STAGE: First observed in the inflorescence meristem (IM) and young flower buds (PubMed:23335616). Detected throughout the floral bud. In young flowers, restricted to the inner whorls, specifically the petals, stamens, and carpels (PubMed:18417639, PubMed:23335616). In older flowers, accumulates more in the stamens than in the petals and carpels (PubMed:18417639). Observed in anther locules, vascular strands, and ovules (PubMed:23335616). During imbibition, expressed in the endosperm, especially at the time of testa rupture. Later restricted to the cotyledons (PubMed:20844019). In mature embryos, restricted to the cotyledons. In young seedlings, mostly expressed in shoot tissues, including the tip, circumference, and vasculature of the cotyledons, the emerging leaves, the meristematic region, and the basal part of the hypocotyl, and, at low levels, in the primary roots. In older seedlings, accumulates in the green shoot tissues (PubMed:22811435). {ECO:0000269\|PubMed:18417639, ECO:0000269\|PubMed:20844019, ECO:0000269\|PubMed:22811435, ECO:0000269\|PubMed:23335616}.
Uniprot	TISSUE SPECIFICITY: Expressed predominantly in leaves, and barely in stems, flowers and siliques. {ECO:0000269\|PubMed:17208962, ECO:0000269\|PubMed:17587690, ECO:0000269\|PubMed:18417639, ECO:0000269\|PubMed:22811435}.

Functional Description ? help Back to Top
Source	Description
TAIR	Encodes a member of the GATA factor family of zinc finger transcription factors.
UniProt	Transcriptional regulator that specifically binds 5'-GATA-3' or 5'-GAT-3' motifs within gene promoters (PubMed:22102866, PubMed:25077795). Involved in the modulation of chloroplast development, growth and division in a cytokinin-dependent manner (PubMed:22102866, PubMed:22811435). Repressor of the gibberellic acid (GA) signaling pathway that regulates flowering and modulates greening, in a SOC1-dependent manner (PubMed:20844019, PubMed:23739688, PubMed:25077795). Prevents the accumulation of SOC1 during flowering (PubMed:23739688). Promotes chlorophyll biosynthesis throughout the plant, by regulating chlorophyll biosynthetic genes (e.g. HEMA1 and GUN4) and chloroplast localized glutamate synthase (e.g. GLU1) (PubMed:18417639, PubMed:20844019, PubMed:21453984, PubMed:22102866, PubMed:23878229, PubMed:25077795). Involved in the regulation of sugar-sensing genes (e.g. HXK1, HXK2, STP13 and PLT6) (PubMed:18417639). Regulator of germination, senescence, elongation growth and flowering time (PubMed:20844019, PubMed:22102866, PubMed:23878229). Influences also leaf starch content (PubMed:22102866). {ECO:0000269\|PubMed:18417639, ECO:0000269\|PubMed:20844019, ECO:0000269\|PubMed:21453984, ECO:0000269\|PubMed:22102866, ECO:0000269\|PubMed:22811435, ECO:0000269\|PubMed:23739688, ECO:0000269\|PubMed:23878229, ECO:0000269\|PubMed:25077795}.

Function -- GeneRIF ? help Back to Top
CGA1 encodes a GATA factor, whose expression is rapidly induced by both the light and cytokinin signals in Arabidopsis thaliana.[CGA1] [PMID: 17587690] GNC and CGA1 regulate chlorophyll production, and positively regulate chloroplast growth in the hypocotyl. [PMID: 22811435] Constitutive Cga1 overexpression increases both chloroplast biogenesis and starch production but also results in delayed senescence and reduced grain filling. Cga1 regulates chloroplast development in higher plants. [PMID: 23548780] a cross-repressive interaction of GNC and GNL on the one side and SOC1 on the other in the control of flowering time, greening, and cold tolerance [PMID: 23739688] Data indicate that CARBON-METABOLISM INVOLVED (GNC) and GNC-LIKE (GNL)/CYTOKININ-RESPONSIVE GATA FACTOR1 (CGA1) expression is repressed by AUXIN RESPONSE FACTO ARF2 and ARF7. [PMID: 23878229]

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT4G26150.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By light (including both red and white lights) (PubMed:17208962, PubMed:17587690). Levels follow a circadian and diurnal rythm, with a peak at 20 hours, thus preempting dawn (PubMed:17208962). Activated by gibberellic acid (GA) (PubMed:20844019). Induced by cytokinin and derivatives (e.g. benzyladenine, t-Zeatin and 6-benzylaminopurine) in light conditions (PubMed:16212609, PubMed:17587690, PubMed:21453984, PubMed:22811435). Triggered by nitrate (PubMed:16262716). Negatively regulated by AP3/PI (PubMed:18417639). Strong accumulation during cold imbibition of nondormant seeds, but not at warm temperatures. Regulated by PIF transcription factors (PubMed:20844019). Repressed by HAN (PubMed:23335616). Inhibited by SOC1 (PubMed:23739688). Down-regulated by auxin (2,4D) and auxin response factors (e.g. ARF2 and ARF7) (PubMed:23878229). {ECO:0000269\|PubMed:16212609, ECO:0000269\|PubMed:17208962, ECO:0000269\|PubMed:17587690, ECO:0000269\|PubMed:18417639, ECO:0000269\|PubMed:20844019, ECO:0000269\|PubMed:21453984, ECO:0000269\|PubMed:22811435, ECO:0000269\|PubMed:23335616, ECO:0000269\|PubMed:23739688, ECO:0000269\|PubMed:23878229, ECO:0000303\|PubMed:16262716}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source	Upstream Regulator (A: Activate/R: Repress)
ATRM	AT3G54340 (R), AT5G20240 (R)

Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source	Target Gene (A: Activate/R: Repress)
ATRM	AT1G57990(A), AT2G15890(A/R), AT2G29350(A), AT4G29130(R), AT4G30270(A), AT4G35770(A), AT5G26340(A/R)

Regulation -- Hormone ? help Back to Top
Source	Hormone
AHD	cytokinin

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT4G26150

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AL049483	0.0	AL049483.1 Arabidopsis thaliana DNA chromosome 4, BAC clone F20B18 (ESSA project).
GenBank	AL161564	0.0	AL161564.2 Arabidopsis thaliana DNA chromosome 4, contig fragment No. 64.
GenBank	CP002687	0.0	CP002687.1 Arabidopsis thaliana chromosome 4 sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_194345.1	0.0	cytokinin-responsive gata factor 1
Swissprot	Q9SZI6	0.0	GAT22_ARATH; Putative GATA transcription factor 22
TrEMBL	A0A178V077	0.0	A0A178V077_ARATH; GNL
STRING	AT4G26150.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM2109	26	75
Representative plant	OGRP68	17	287

Link Out ? help Back to Top
Phytozome	AT4G26150.1
Entrez Gene	828721
iHOP	AT4G26150
wikigenes	AT4G26150

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Jeong MJ,Jeong MJ,Shih MC Interaction of a GATA factor with cis-acting elements involved in light regulation of nuclear genes encoding chloroplast glyceraldehyde-3-phosphate dehydrogenase in Arabidopsis. Biochem. Biophys. Res. Commun., 2003. 300(2): p. 555-62 [PMID:12504119] Reyes JC,Muro-Pastor MI,Florencio FJ The GATA family of transcription factors in Arabidopsis and rice. Plant Physiol., 2004. 134(4): p. 1718-32 [PMID:15084732] S ABA activates ADPR cyclase and cADPR induces a subset of ABA-responsive genes in Arabidopsis. Plant J., 2004. 38(3): p. 381-95 [PMID:15086800] Wang R, et al. Genomic analysis of the nitrate response using a nitrate reductase-null mutant of Arabidopsis. Plant Physiol., 2004. 136(1): p. 2512-22 [PMID:15333754] Scheible WR, et al. Genome-wide reprogramming of primary and secondary metabolism, protein synthesis, cellular growth processes, and the regulatory infrastructure of Arabidopsis in response to nitrogen. Plant Physiol., 2004. 136(1): p. 2483-99 [PMID:15375205] Kiba T, et al. Combinatorial microarray analysis revealing arabidopsis genes implicated in cytokinin responses through the His->Asp Phosphorelay circuitry. Plant Cell Physiol., 2005. 46(2): p. 339-55 [PMID:15695462] Brenner WG,Romanov GA,K Immediate-early and delayed cytokinin response genes of Arabidopsis thaliana identified by genome-wide expression profiling reveal novel cytokinin-sensitive processes and suggest cytokinin action through transcriptional cascades. Plant J., 2005. 44(2): p. 314-33 [PMID:16212609] Bi YM, et al. Genetic analysis of Arabidopsis GATA transcription factor gene family reveals a nitrate-inducible member important for chlorophyll synthesis and glucose sensitivity. Plant J., 2005. 44(4): p. 680-92 [PMID:16262716] Che P,Lall S,Nettleton D,Howell SH Gene expression programs during shoot, root, and callus development in Arabidopsis tissue culture. Plant Physiol., 2006. 141(2): p. 620-37 [PMID:16648215] Nemhauser JL,Hong F,Chory J Different plant hormones regulate similar processes through largely nonoverlapping transcriptional responses. Cell, 2006. 126(3): p. 467-75 [PMID:16901781] Lee DJ, et al. Genome-wide expression profiling of ARABIDOPSIS RESPONSE REGULATOR 7(ARR7) overexpression in cytokinin response. Mol. Genet. Genomics, 2007. 277(2): p. 115-37 [PMID:17061125] Manfield IW,Devlin PF,Jen CH,Westhead DR,Gilmartin PM Conservation, convergence, and divergence of light-responsive, circadian-regulated, and tissue-specific expression patterns during evolution of the Arabidopsis GATA gene family. Plant Physiol., 2007. 143(2): p. 941-58 [PMID:17208962] Osuna D, et al. Temporal responses of transcripts, enzyme activities and metabolites after adding sucrose to carbon-deprived Arabidopsis seedlings. Plant J., 2007. 49(3): p. 463-91 [PMID:17217462] Kleine T,Kindgren P,Benedict C,Hendrickson L,Strand A Genome-wide gene expression analysis reveals a critical role for CRYPTOCHROME1 in the response of Arabidopsis to high irradiance. Plant Physiol., 2007. 144(3): p. 1391-406 [PMID:17478635] Naito T,Kiba T,Koizumi N,Yamashino T,Mizuno T Characterization of a unique GATA family gene that responds to both light and cytokinin in Arabidopsis thaliana. Biosci. Biotechnol. Biochem., 2007. 71(6): p. 1557-60 [PMID:17587690] Chevalier F, et al. GeBP and GeBP-like proteins are noncanonical leucine-zipper transcription factors that regulate cytokinin response in Arabidopsis. Plant Physiol., 2008. 146(3): p. 1142-54 [PMID:18162594] Mara CD,Irish VF Two GATA transcription factors are downstream effectors of floral homeotic gene action in Arabidopsis. Plant Physiol., 2008. 147(2): p. 707-18 [PMID:18417639] Richter R,Behringer C,M The GATA-type transcription factors GNC and GNL/CGA1 repress gibberellin signaling downstream from DELLA proteins and PHYTOCHROME-INTERACTING FACTORS. Genes Dev., 2010. 24(18): p. 2093-104 [PMID:20844019] K Ectopic expression of different cytokinin-regulated transcription factor genes of Arabidopsis thaliana alters plant growth and development. J. Plant Physiol., 2011. 168(12): p. 1320-7 [PMID:21453984] Hudson D, et al. GNC and CGA1 modulate chlorophyll biosynthesis and glutamate synthase (GLU1/Fd-GOGAT) expression in Arabidopsis. PLoS ONE, 2011. 6(11): p. e26765 [PMID:22102866] Chiang YH, et al. Functional characterization of the GATA transcription factors GNC and CGA1 reveals their key role in chloroplast development, growth, and division in Arabidopsis. Plant Physiol., 2012. 160(1): p. 332-48 [PMID:22811435] Zhang X, et al. Transcription repressor HANABA TARANU controls flower development by integrating the actions of multiple hormones, floral organ specification genes, and GATA3 family genes in Arabidopsis. Plant Cell, 2013. 25(1): p. 83-101 [PMID:23335616] Hudson D, et al. Rice cytokinin GATA transcription Factor1 regulates chloroplast development and plant architecture. Plant Physiol., 2013. 162(1): p. 132-44 [PMID:23548780] Richter R,Bastakis E,Schwechheimer C Cross-repressive interactions between SOC1 and the GATAs GNC and GNL/CGA1 in the control of greening, cold tolerance, and flowering time in Arabidopsis. Plant Physiol., 2013. 162(4): p. 1992-2004 [PMID:23739688] Richter R,Behringer C,Zourelidou M,Schwechheimer C Convergence of auxin and gibberellin signaling on the regulation of the GATA transcription factors GNC and GNL in Arabidopsis thaliana. Proc. Natl. Acad. Sci. U.S.A., 2013. 110(32): p. 13192-7 [PMID:23878229] Behringer C,Bastakis E,Ranftl QL,Mayer KF,Schwechheimer C Functional diversification within the family of B-GATA transcription factors through the leucine-leucine-methionine domain. Plant Physiol., 2014. 166(1): p. 293-305 [PMID:25077795] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178] Behringer C,Schwechheimer C B-GATA transcription factors - insights into their structure, regulation, and role in plant development. Front Plant Sci, 2015. 6: p. 90 [PMID:25755661] Ranftl QL,Bastakis E,Klermund C,Schwechheimer C LLM-Domain Containing B-GATA Factors Control Different Aspects of Cytokinin-Regulated Development in Arabidopsis thaliana. Plant Physiol., 2016. 170(4): p. 2295-311 [PMID:26829982] Klermund C, et al. LLM-Domain B-GATA Transcription Factors Promote Stomatal Development Downstream of Light Signaling Pathways in Arabidopsis thaliana Hypocotyls. Plant Cell, 2016. 28(3): p. 646-60 [PMID:26917680] Kobayashi K, et al. Shoot Removal Induces Chloroplast Development in Roots via Cytokinin Signaling. Plant Physiol., 2017. 173(4): p. 2340-2355 [PMID:28193764] Kobayashi K,Iwase A Simultaneous but spatially different regulation of non-photosynthetic callus formation and photosynthetic root development after shoot removal. Plant Signal Behav, 2017. 12(6): p. e1338999 [PMID:28594268] Bastakis E,Hedtke B,Klermund C,Grimm B,Schwechheimer C LLM-Domain B-GATA Transcription Factors Play Multifaceted Roles in Controlling Greening in Arabidopsis. Plant Cell, 2018. 30(3): p. 582-599 [PMID:29453227]