PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT4G17750.1

Common Name

ATHSF1, ATHSFA1A, dl4910c, FCAALL.107, HSF1, HSF13, HSFA1A

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

HSF

Protein Properties

Length: 495aa MW: 55744 Da PI: 4.8908

Description

heat shock factor 1

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT4G17750.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HSF_DNA-bind	117.6	7.6e-37	53	144	2	102
HHHHHHHHHCTGGGTTTSEESSSSSEEEES-HHHHHHHTHHHHSTT--HHHHHHHHHHTTEEE---SSBTTTTXTTSEEEEESXXXXXXXXXXXXXX CS HSF_DNA-bind 2 FlkklyeiledeelkeliswsengnsfvvldeeefakkvLpkyFkhsnfaSFvRQLnmYgFkkvkdeekkskskekiweFkhksFkkgkkellekik 98 Fl+k+y+++ed++++ ++sws ++nsf+v+d+ ef++++LpkyFkh+nf+SFvRQLn+YgF+kv+ ++ weF+++ F +g+k+ll+ki AT4G17750.1 53 FLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSFVRQLNTYGFRKVDPDR---------WEFANEGFLRGQKHLLKKIS 140 9******************999*************************************999.........**************** PP XXXX CS HSF_DNA-bind 99 rkks 102 r+ks AT4G17750.1 141 RRKS 144 97 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SMART	SM00415	1.1E-63	49	142	IPR000232	Heat shock factor (HSF)-type, DNA-binding
Gene3D	G3DSA:1.10.10.10	3.0E-40	50	142	IPR011991	Winged helix-turn-helix DNA-binding domain
SuperFamily	SSF46785	1.12E-35	50	142	IPR011991	Winged helix-turn-helix DNA-binding domain
PRINTS	PR00056	1.2E-20	53	76	IPR000232	Heat shock factor (HSF)-type, DNA-binding
Pfam	PF00447	6.0E-33	53	142	IPR000232	Heat shock factor (HSF)-type, DNA-binding
PRINTS	PR00056	1.2E-20	91	103	IPR000232	Heat shock factor (HSF)-type, DNA-binding
PROSITE pattern	PS00434	0	92	116	IPR000232	Heat shock factor (HSF)-type, DNA-binding
PRINTS	PR00056	1.2E-20	104	116	IPR000232	Heat shock factor (HSF)-type, DNA-binding

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009408	Biological Process	response to heat
GO:0005634	Cellular Component	nucleus
GO:0005737	Cellular Component	cytoplasm
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0005515	Molecular Function	protein binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000084	anatomy	plant sperm cell
PO:0000293	anatomy	guard cell
PO:0009005	anatomy	root
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0025022	anatomy	collective leaf structure
PO:0025281	anatomy	pollen
PO:0001017	developmental stage	M germinated pollen stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 495 aa Download sequence Send to blast
MFVNFKYFSF FIRTKMDGVT GGGTNIGEAV TAPPPRNPHP ATLLNANSLP PPFLSKTYDM 60 VEDPATDAIV SWSPTNNSFI VWDPPEFSRD LLPKYFKHNN FSSFVRQLNT YGFRKVDPDR 120 WEFANEGFLR GQKHLLKKIS RRKSVQGHGS SSSNPQSQQL SQGQGSMAAL SSCVEVGKFG 180 LEEEVEQLKR DKNVLMQELV KLRQQQQTTD NKLQVLVKHL QVMEQRQQQI MSFLAKAVQN 240 PTFLSQFIQK QTDSNMHVTE ANKKRRLRED STAATESNSH SHSLEASDGQ IVKYQPLRND 300 SMMWNMMKTD DKYPFLDGFS SPNQVSGVTL QEVLPITSGQ SQAYASVPSG QPLSYLPSTS 360 TSLPDTIMPE TSQIPQLTRE SINDFPTENF MDTEKNVPEA FISPSPFLDG GSVPIQLEGI 420 PEDPEIDELM SNFEFLEEYM PESPVFGDAT TLENNNNNNN NNNNNNNNNN NNNTNGRHMD 480 KLIEELGLLT SETEH

Sequence ? help Back to Top

Protein Sequence Length: 495 aa Download sequence Send to blast

MFVNFKYFSF FIRTKMDGVT GGGTNIGEAV TAPPPRNPHP ATLLNANSLP PPFLSKTYDM  60
VEDPATDAIV SWSPTNNSFI VWDPPEFSRD LLPKYFKHNN FSSFVRQLNT YGFRKVDPDR  120
WEFANEGFLR GQKHLLKKIS RRKSVQGHGS SSSNPQSQQL SQGQGSMAAL SSCVEVGKFG  180
LEEEVEQLKR DKNVLMQELV KLRQQQQTTD NKLQVLVKHL QVMEQRQQQI MSFLAKAVQN  240
PTFLSQFIQK QTDSNMHVTE ANKKRRLRED STAATESNSH SHSLEASDGQ IVKYQPLRND  300
SMMWNMMKTD DKYPFLDGFS SPNQVSGVTL QEVLPITSGQ SQAYASVPSG QPLSYLPSTS  360
TSLPDTIMPE TSQIPQLTRE SINDFPTENF MDTEKNVPEA FISPSPFLDG GSVPIQLEGI  420
PEDPEIDELM SNFEFLEEYM PESPVFGDAT TLENNNNNNN NNNNNNNNNN NNNTNGRHMD  480
KLIEELGLLT SETEH

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ldu_A	2e-28	51	142	18	120	Heat shock factor protein 1
Search in ModeBase

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.32971	0.0	flower

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	30684174	0.0
Genevisible	245379_at	0.0
Expression Atlas	AT4G17750	-
AtGenExpress	AT4G17750	-
ATTED-II	AT4G17750	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Constitutively expressed. {ECO:0000269\|PubMed:7948881}.

Functional Description ? help Back to Top
Source	Description
TAIR	native protein is a trimer, interacts with HSP70, also with TBP, DNA interaction is modulated by phosphorylation and is heat-shock inducible
UniProt	Transcriptional activator that specifically binds DNA sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE).

Function -- GeneRIF ? help Back to Top
Results revealed that overexpressing HsfA1a had positive effects on tolerance to diverse stressors by promoting inducible Hsp expression following stress-induced HsfA1a activation. [PMID: 24634180]

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT4G17750.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By heat stress. {ECO:0000269\|PubMed:7948881}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source	Target Gene (A: Activate/R: Repress)
ATRM	AT1G56600(A), AT2G22240(A), AT2G47180(A), AT5G12030(A)

Interaction ? help Back to Top
Source	Intact With
BioGRID	AT4G17750, AT5G16820
IntAct	Search P41151

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT4G17750

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AK229347	0.0	AK229347.1 Arabidopsis thaliana mRNA for heat shock transcription factor HSF1, partial cds, clone: RAFL16-59-L13.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_193510.1	0.0	heat shock factor 1
Swissprot	P41151	0.0	HFA1A_ARATH; Heat stress transcription factor A-1a
TrEMBL	A0A178UWX9	0.0	A0A178UWX9_ARATH; HSFA1A
STRING	AT4G17750.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM15101	16	19
Representative plant	OGRP96	17	233

Link Out ? help Back to Top
Phytozome	AT4G17750.1
Entrez Gene	827496
iHOP	AT4G17750
wikigenes	AT4G17750

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Nover L, et al. Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need? Cell Stress Chaperones, 2001. 6(3): p. 177-89 [PMID:11599559] Zhang L,Eggers-Schumacher G,Sch Analysis of heat-shock transcription factor-DNA binding in Arabidopsis suspension cultures by UV laser crosslinking. Plant J., 2001. 28(2): p. 217-23 [PMID:11722765] Kim BH,Schöffl F Interaction between Arabidopsis heat shock transcription factor 1 and 70 kDa heat shock proteins. J. Exp. Bot., 2002. 53(367): p. 371-5 [PMID:11807141] Zhang L,Lohmann C,Pr Heat stress-dependent DNA binding of Arabidopsis heat shock transcription factor HSF1 to heat shock gene promoters in Arabidopsis suspension culture cells in vivo. Biol. Chem., 2003. 384(6): p. 959-63 [PMID:12887064] Wunderlich M,Werr W,Sch Generation of dominant-negative effects on the heat shock response in Arabidopsis thaliana by transgenic expression of a chimaeric HSF1 protein fusion construct. Plant J., 2003. 35(4): p. 442-51 [PMID:12904207] Lohmann C,Eggers-Schumacher G,Wunderlich M,Sch Two different heat shock transcription factors regulate immediate early expression of stress genes in Arabidopsis. Mol. Genet. Genomics, 2004. 271(1): p. 11-21 [PMID:14655047] Czarnecka-Verner E,Pan S,Salem T,Gurley WB Plant class B HSFs inhibit transcription and exhibit affinity for TFIIB and TBP. Plant Mol. Biol., 2004. 56(1): p. 57-75 [PMID:15604728] Busch W,Wunderlich M,Sch Identification of novel heat shock factor-dependent genes and biochemical pathways in Arabidopsis thaliana. Plant J., 2005. 41(1): p. 1-14 [PMID:15610345] Baniwal SK, et al. Heat stress response in plants: a complex game with chaperones and more than twenty heat stress transcription factors. J. Biosci., 2004. 29(4): p. 471-87 [PMID:15625403] Fu S,Rogowsky P,Nover L,Scanlon MJ The maize heat shock factor-binding protein paralogs EMP2 and HSBP2 interact non-redundantly with specific heat shock factors. Planta, 2006. 224(1): p. 42-52 [PMID:16331466] Nishizawa A, et al. Arabidopsis heat shock transcription factor A2 as a key regulator in response to several types of environmental stress. Plant J., 2006. 48(4): p. 535-47 [PMID:17059409] Liu HT, et al. Calmodulin-binding protein phosphatase PP7 is involved in thermotolerance in Arabidopsis. Plant Cell Environ., 2007. 30(2): p. 156-64 [PMID:17238907] Guo J, et al. Genome-wide analysis of heat shock transcription factor families in rice and Arabidopsis. J Genet Genomics, 2008. 35(2): p. 105-18 [PMID:18407058] Liu HT, et al. The calmodulin-binding protein kinase 3 is part of heat-shock signal transduction in Arabidopsis thaliana. Plant J., 2008. 55(5): p. 760-73 [PMID:18466301] Nishizawa A,Yabuta Y,Shigeoka S Galactinol and raffinose constitute a novel function to protect plants from oxidative damage. Plant Physiol., 2008. 147(3): p. 1251-63 [PMID:18502973] Guo L, et al. Isolation of heat shock factor HsfA1a-binding sites in vivo revealed variations of heat shock elements in Arabidopsis thaliana. Plant Cell Physiol., 2008. 49(9): p. 1306-15 [PMID:18641404] Li M, et al. Detection of in vivo interactions between Arabidopsis class A-HSFs, using a novel BiFC fragment, and identification of novel class B-HSF interacting proteins. Eur. J. Cell Biol., 2010 Feb-Mar. 89(2-3): p. 126-32 [PMID:19945192] Banti V,Mafessoni F,Loreti E,Alpi A,Perata P The heat-inducible transcription factor HsfA2 enhances anoxia tolerance in Arabidopsis. Plant Physiol., 2010. 152(3): p. 1471-83 [PMID:20089772] Xu X, et al. Functional characterization of AtHsp90.3 in Saccharomyces cerevisiae and Arabidopsis thaliana under heat stress. Biotechnol. Lett., 2010. 32(7): p. 979-87 [PMID:20229063] Hsu SF,Lai HC,Jinn TL Cytosol-localized heat shock factor-binding protein, AtHSBP, functions as a negative regulator of heat shock response by translocation to the nucleus and is required for seed development in Arabidopsis. Plant Physiol., 2010. 153(2): p. 773-84 [PMID:20388662] Li M,Berendzen KW,Sch Promoter specificity and interactions between early and late Arabidopsis heat shock factors. Plant Mol. Biol., 2010. 73(4-5): p. 559-67 [PMID:20458611] Hsu SF,Jinn TL AtHSBP functions in seed development and the motif is required for subcellular localization and interaction with AtHSFs. Plant Signal Behav, 2010. 5(8): p. 1042-4 [PMID:20657173] Shin R,Jez JM,Basra A,Zhang B,Schachtman DP 14-3-3 proteins fine-tune plant nutrient metabolism. FEBS Lett., 2011. 585(1): p. 143-7 [PMID:21094157] Yoshida T, et al. Arabidopsis HsfA1 transcription factors function as the main positive regulators in heat shock-responsive gene expression. Mol. Genet. Genomics, 2011. 286(5-6): p. 321-32 [PMID:21931939] Liu HC,Charng YY Acquired thermotolerance independent of heat shock factor A1 (HsfA1), the master regulator of the heat stress response. Plant Signal Behav, 2012. 7(5): p. 547-50 [PMID:22516818] Liu Y, et al. Arabidopsis heat shock factor HsfA1a directly senses heat stress, pH changes, and hydrogen peroxide via the engagement of redox state. Plant Physiol. Biochem., 2013. 64: p. 92-8 [PMID:23399534] Liu HC,Charng YY Common and distinct functions of Arabidopsis class A1 and A2 heat shock factors in diverse abiotic stress responses and development. Plant Physiol., 2013. 163(1): p. 276-90 [PMID:23832625] Qian J, et al. Overexpression of Arabidopsis HsfA1a enhances diverse stress tolerance by promoting stress-induced Hsp expression. Genet. Mol. Res., 2014. 13(1): p. 1233-43 [PMID:24634180] Li S, et al. HEAT-INDUCED TAS1 TARGET1 Mediates Thermotolerance via HEAT STRESS TRANSCRIPTION FACTOR A1a-Directed Pathways in Arabidopsis. Plant Cell, 2014. 26(4): p. 1764-1780 [PMID:24728648] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178] Muench M,Hsin CH,Ferber E,Berger S,Mueller MJ Reactive electrophilic oxylipins trigger a heat stress-like response through HSFA1 transcription factors. J. Exp. Bot., 2016. 67(21): p. 6139-6148 [PMID:27811081] Lee JH,H Derepression of the activity of genetically engineered heat shock factor causes constitutive synthesis of heat shock proteins and increased thermotolerance in transgenic Arabidopsis. Plant J., 1995. 8(4): p. 603-12 [PMID:7496404] H Arabidopsis heat shock factor is constitutively active in Drosophila and human cells. Mol. Gen. Genet., 1995. 248(2): p. 136-41 [PMID:7651336] H Arabidopsis heat shock factor: isolation and characterization of the gene and the recombinant protein. Plant Mol. Biol., 1994. 26(1): p. 353-62 [PMID:7948881] Reindl A,Schöffl F,Schell J,Koncz C,Bakó L Phosphorylation by a cyclin-dependent kinase modulates DNA binding of the Arabidopsis heat-shock transcription factor HSF1 in vitro. Plant Physiol., 1997. 115(1): p. 93-100 [PMID:9306693] Reindl A,Schöffl F Interaction between the Arabidopsis thaliana heat shock transcription factor HSF1 and the TATA binding protein TBP. FEBS Lett., 1998. 436(3): p. 318-22 [PMID:9801140]