PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT3G58070.1

Common Name

GIS, T10K17.280

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

C2H2

Protein Properties

Length: 253aa MW: 28456.8 Da PI: 7.7141

Description

C2H2 family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT3G58070.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	zf-C2H2	15.4	5.2e-05	91	113	1	23
EEETTTTEEESSHHHHHHHHHHT CS zf-C2H2 1 ykCpdCgksFsrksnLkrHirtH 23 ++C++C + F+++ L H++ H AT3G58070.1 91 FECHYCFRNFPTSQALGGHQNAH 113 89******************988 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF57667	1.06E-5	88	118	No hit	No description
Pfam	PF13912	2.0E-5	90	115	IPR007087	Zinc finger, C2H2
PROSITE profile	PS50157	10.99	91	118	IPR007087	Zinc finger, C2H2
PROSITE pattern	PS00028	0	93	113	IPR007087	Zinc finger, C2H2

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0007275	Biological Process	multicellular organism development
GO:0009739	Biological Process	response to gibberellin
GO:0010091	Biological Process	trichome branching
GO:0003676	Molecular Function	nucleic acid binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0008270	Molecular Function	zinc ion binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000014	anatomy	rosette leaf
PO:0000037	anatomy	shoot apex
PO:0000112	anatomy	shoot axis epidermis
PO:0000229	anatomy	flower meristem
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0008019	anatomy	leaf lamina base
PO:0009006	anatomy	shoot system
PO:0009025	anatomy	vascular leaf
PO:0009047	anatomy	stem
PO:0020038	anatomy	petiole
PO:0025022	anatomy	collective leaf structure
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0007064	developmental stage	LP.12 twelve leaves visible stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage

Sequence ? help Back to Top
Protein Sequence Length: 253 aa Download sequence Send to blast
MDEATGETET QDFMNVESFS QLPFIRRPKD KNPKPIRVFG KDFTGRDFSI TTGQEDYTDP 60 YQTKNKEEEE EEDQTGDNST DNNSISHNRR FECHYCFRNF PTSQALGGHQ NAHKRERQLA 120 KRGVSSYFYH PDNNPYSYRH YPSWTNGPLT AARSYGGFSS GPKPSGYYTR PSYGSQLGLW 180 RLPPRVQGVY NSNAAFTSNG SSSSSNSTLP LLTRSQTQLS SQVGGSAAQN RMSSYGYGLS 240 PNVQDHVSLD LHL

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	30694762	0.0
Genevisible	251586_at	0.0
Expression Atlas	AT3G58070	-
AtGenExpress	AT3G58070	-
ATTED-II	AT3G58070	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed in inflorescence meristems, floral meristems and stem epidermis. {ECO:0000269\|PubMed:16679458}.

Functional Description ? help Back to Top
Source	Description
TAIR	Putative transcription factor, contains C2H2 domain, regulates aspects of shoot maturation in Arabidopsis thaliana. GIS loss-of-function mutations affect the epidermal differentiation of inflorescence organs, causing a premature decrease in trichome production on successive leaves, stem internodes, and branches. Overexpression has the opposite effect on trichome initiation and causes other heterochronic phenotypes, affecting flowering and juvenileadult leaf transition and inducing the formation of rosette leaves on inflorescence stems.
UniProt	Probable transcription factor required for the initiation of inflorescence trichomes in response to gibberellin (GA) (PubMed:16679458, PubMed:17507408). Mediates the induction of GL1 expression by GA in inflorescence organs and is antagonized in its action by the DELLA repressor GAI. Acts upstream of the trichome initiation regulators GL1 and GL3, and downstream of the GA signaling repressor SPINDLY (SPY) (PubMed:16679458). Does not play a significant role in the cytokinin response (PubMed:17507408). Controls trichome branching through GA signaling (PubMed:22210898, PubMed:23825141). Acts downstream of the key regulator STICHEL (STI) in an endoreduplication-independent pathway (PubMed:22210898). Controls trichome cell division indirectly by acting downstream of a key endoreduplication regulator SIAMESE (SIM) (PubMed:23825141). {ECO:0000269\|PubMed:16679458, ECO:0000269\|PubMed:17507408, ECO:0000269\|PubMed:22210898, ECO:0000269\|PubMed:23825141}.

Function -- GeneRIF ? help Back to Top
GLABROUS INFLORESCENCE STEMS (GIS) is a putative C2H2 transcription factor which regulates several aspects of shoot maturation in Arabidopsis. [GIS] [PMID: 16679458] GIS is required for the gibberellic acid-regulated trichome branching. GIS is not required for cell endoreduplication but functions downstream of the key regulator STI to control trichome branching in an endoreduplication-independent pathway. [PMID: 22210898] show that ZFP5 encodes a protein functionally equivalent to GIS and GIS2 in controlling trichome initiation [PMID: 22301962] GIS may play an indirect role in regulating trichome cell division by genetically interacting with SIM [PMID: 23825141] Detailed genetic analyses show that GIS and TCP4 work in same pathway and GIS function is required for TCP4-mediated regulation of trichome differentiation. [PMID: 29165850]

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT3G58070.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By gibberellin. {ECO:0000269\|PubMed:16679458}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source	Upstream Regulator (A: Activate/R: Repress)
ATRM	AT2G01570 (A)

Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source	Target Gene (A: Activate/R: Repress)
ATRM	AT3G27920(A), AT5G41315(A)

Regulation -- Hormone ? help Back to Top
Source	Hormone
AHD	gibberellin

Phenotype -- Disruption Phenotype ? help Back to Top
Source	Description
UniProt	DISRUPTION PHENOTYPE: Reduced trichome production on cauline leaves, stem internodes and branches, and sepals. {ECO:0000269\|PubMed:16679458, ECO:0000269\|PubMed:17507408, ECO:0000269\|PubMed:22210898}.

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT3G58070

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AL137081	0.0	AL137081.1 Arabidopsis thaliana DNA chromosome 3, BAC clone F9D24.
GenBank	BT003340	0.0	BT003340.1 Arabidopsis thaliana zinc finger-like protein (At3g58070) mRNA, complete cds.
GenBank	BT009676	0.0	BT009676.1 Arabidopsis thaliana At3g58070 mRNA, complete cds.
GenBank	CP002686	0.0	CP002686.1 Arabidopsis thaliana chromosome 3, complete sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_191366.1	0.0	C2H2 and C2HC zinc fingers superfamily protein
Swissprot	Q84WI0	0.0	GIS_ARATH; Zinc finger protein GIS
TrEMBL	A0A178V7D1	0.0	A0A178V7D1_ARATH; GIS
STRING	AT3G58070.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM2495	19	49
Representative plant	OGRP2859	5	8

Link Out ? help Back to Top
Phytozome	AT3G58070.1
Entrez Gene	824976
iHOP	AT3G58070
wikigenes	AT3G58070

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Czechowski T,Bari RP,Stitt M,Scheible WR,Udvardi MK Real-time RT-PCR profiling of over 1400 Arabidopsis transcription factors: unprecedented sensitivity reveals novel root- and shoot-specific genes. Plant J., 2004. 38(2): p. 366-79 [PMID:15078338] Englbrecht CC,Schoof H,B Conservation, diversification and expansion of C2H2 zinc finger proteins in the Arabidopsis thaliana genome. BMC Genomics, 2004. 5(1): p. 39 [PMID:15236668] Kiba T, et al. Combinatorial microarray analysis revealing arabidopsis genes implicated in cytokinin responses through the His->Asp Phosphorelay circuitry. Plant Cell Physiol., 2005. 46(2): p. 339-55 [PMID:15695462] Nagata T,Yamada H,Du Z,Todoriki S,Kikuchi S Microarray analysis of genes that respond to gamma-irradiation in Arabidopsis. J. Agric. Food Chem., 2005. 53(4): p. 1022-30 [PMID:15713015] Gan Y, et al. GLABROUS INFLORESCENCE STEMS modulates the regulation by gibberellins of epidermal differentiation and shoot maturation in Arabidopsis. Plant Cell, 2006. 18(6): p. 1383-95 [PMID:16679458] Xin Z,Mandaokar A,Chen J,Last RL,Browse J Arabidopsis ESK1 encodes a novel regulator of freezing tolerance. Plant J., 2007. 49(5): p. 786-99 [PMID:17316173] Gan Y,Liu C,Yu H,Broun P Integration of cytokinin and gibberellin signalling by Arabidopsis transcription factors GIS, ZFP8 and GIS2 in the regulation of epidermal cell fate. Development, 2007. 134(11): p. 2073-81 [PMID:17507408] Gan Y,Yu H,Peng J,Broun P Genetic and molecular regulation by DELLA proteins of trichome development in Arabidopsis. Plant Physiol., 2007. 145(3): p. 1031-42 [PMID:17704233] Ciftci-Yilmaz S,Mittler R The zinc finger network of plants. Cell. Mol. Life Sci., 2008. 65(7-8): p. 1150-60 [PMID:18193167] Yu N, et al. Temporal control of trichome distribution by microRNA156-targeted SPL genes in Arabidopsis thaliana. Plant Cell, 2010. 22(7): p. 2322-35 [PMID:20622149] Zhou Z, et al. Zinc finger protein5 is required for the control of trichome initiation by acting upstream of zinc finger protein8 in Arabidopsis. Plant Physiol., 2011. 157(2): p. 673-82 [PMID:21803862] An L,Zhou Z,Su S,Yan A,Gan Y GLABROUS INFLORESCENCE STEMS (GIS) is required for trichome branching through gibberellic acid signaling in Arabidopsis. Plant Cell Physiol., 2012. 53(2): p. 457-69 [PMID:22210898] Zhou Z,An L,Sun L,Gan Y ZFP5 encodes a functionally equivalent GIS protein to control trichome initiation. Plant Signal Behav, 2012. 7(1): p. 28-30 [PMID:22301962] Yan A,Pan J,An L,Gan Y,Feng H The responses of trichome mutants to enhanced ultraviolet-B radiation in Arabidopsis thaliana. J. Photochem. Photobiol. B, Biol., 2012. 113: p. 29-35 [PMID:22647943] Meinke DW A survey of dominant mutations in Arabidopsis thaliana. Trends Plant Sci., 2013. 18(2): p. 84-91 [PMID:22995285] Zhou Z, et al. Zinc Finger Protein 6 (ZFP6) regulates trichome initiation by integrating gibberellin and cytokinin signaling in Arabidopsis thaliana. New Phytol., 2013. 198(3): p. 699-708 [PMID:23506479] Sun LL, et al. GLABROUS INFLORESCENCE STEMS regulates trichome branching by genetically interacting with SIM in Arabidopsis. J Zhejiang Univ Sci B, 2013. 14(7): p. 563-9 [PMID:23825141] Sun L, et al. GLABROUS INFLORESCENCE STEMS3 (GIS3) regulates trichome initiation and development in Arabidopsis. New Phytol., 2015. 206(1): p. 220-30 [PMID:25640859] Yu D, et al. RPN1a, a subunit of the 26S proteasome, controls trichome development in Arabidopsis. Plant Physiol. Biochem., 2015. 88: p. 82-8 [PMID:25676129] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178] Liu Y, et al. AtGIS, a C2H2 zinc-finger transcription factor from Arabidopsis regulates glandular trichome development through GA signaling in tobacco. Biochem. Biophys. Res. Commun., 2017. 483(1): p. 209-215 [PMID:28034756] Vadde BVL,Challa KR,Nath U The TCP4 transcription factor regulates trichome cell differentiation by directly activating GLABROUS INFLORESCENCE STEMS in Arabidopsis thaliana. Plant J., 2018. 93(2): p. 259-269 [PMID:29165850] Kim SY,Hyoung S,So WM,Shin JS The novel transcription factor TRP interacts with ZFP5, a trichome initiation-related transcription factor, and negatively regulates trichome initiation through gibberellic acid signaling. Plant Mol. Biol., 2018. 96(3): p. 315-326 [PMID:29335898]