PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT3G28910.1
Common NameATMYB30, hsr1, MLD15.8, MYB30
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family MYB
Protein Properties Length: 323aa    MW: 35918.9 Da    PI: 8.068
Description myb domain protein 30
Gene Model
Gene Model ID Type Source Coding Sequence
AT3G28910.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
  Myb_DNA-binding  1 rgrWTteEdellvdavkqlGggtWktIartmgkgRtlkqcksrwqkyl 48
                     +g+WT+eEd +lv +++++G+g+W++++   g+ R+ k+c++rw +yl
                     79******************************99************97 PP

  Myb_DNA-binding   1 rgrWTteEdellvdavkqlGggtWktIartmgkgRtlkqcksrwqkyl 48 
                      rg++T+ E++ +v++ ++lG++ W++Ia++++  Rt++++k++w+++l
                      89********************.*********.************996 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS5129417.078961IPR017930Myb domain
SMARTSM007171.6E-141363IPR001005SANT/Myb domain
PfamPF002495.6E-161461IPR001005SANT/Myb domain
CDDcd001679.55E-111661No hitNo description
PROSITE profilePS5129423.73962116IPR017930Myb domain
SMARTSM007172.5E-1566114IPR001005SANT/Myb domain
PfamPF002493.1E-1467112IPR001005SANT/Myb domain
CDDcd001672.74E-1069112No hitNo description
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0001666Biological Processresponse to hypoxia
GO:0006357Biological Processregulation of transcription from RNA polymerase II promoter
GO:0009617Biological Processresponse to bacterium
GO:0009626Biological Processplant-type hypersensitive response
GO:0009723Biological Processresponse to ethylene
GO:0009733Biological Processresponse to auxin
GO:0009739Biological Processresponse to gibberellin
GO:0009751Biological Processresponse to salicylic acid
GO:0009753Biological Processresponse to jasmonic acid
GO:0030154Biological Processcell differentiation
GO:0042761Biological Processvery long-chain fatty acid biosynthetic process
GO:0005634Cellular Componentnucleus
GO:0000981Molecular FunctionRNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0001135Molecular Functiontranscription factor activity, RNA polymerase II transcription factor recruiting
GO:0005515Molecular Functionprotein binding
GO:0043565Molecular Functionsequence-specific DNA binding
GO:0044212Molecular Functiontranscription regulatory region DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000263anatomynon-hair root epidermal cell
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009025anatomyvascular leaf
PO:0009052anatomyflower pedicel
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 323 aa     Download sequence    Send to blast
3D Structure ? help Back to Top
PDB ID Evalue Query Start Query End Hit Start Hit End Description
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.109020.0flower| root| seed| silique
Expression -- Microarray ? help Back to Top
Source ID E-value
Expression AtlasAT3G28910-
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: Expressed in 2-week-old seedlings, in the early stages of development. {ECO:0000269|PubMed:10571865}.
UniprotTISSUE SPECIFICITY: Expressed in vascular tissues of leaves, hypocotyl and roots. {ECO:0000269|PubMed:24587042}.
Functional Description ? help Back to Top
Source Description
TAIRtranscription factor myb homologue
UniProtTranscription factor that binds specifically to the DNA sequence 5'-AACAAAC-3' (PubMed:19170933). Acts as a positive regulator of hypersensitive cell death (PubMed:10571865, PubMed:12119395). Acts as a positive regulator of salicylic acid synthesis (PubMed:16730712). Regulates very-long-chain fatty acid biosynthesis (PubMed:18326828). Acts cooperatively with BZR2 to promote expression of a subset of brassinosteroids target genes (PubMed:19170933). Transcriptional activity and hypersensitive response control negatively regulated by PLA2-ALPHA and by the Xanthomonas type III effector XopD (AC G9L9K6) (PubMed:20696912, PubMed:21917550). Involved in the regulation of abscisic acid (ABA) signaling (PubMed:22814374). Increased levels of MYB30 can accelerate flowering both in long and short days through the regulation of FT (PubMed:24587042). {ECO:0000269|PubMed:10571865, ECO:0000269|PubMed:12119395, ECO:0000269|PubMed:16730712, ECO:0000269|PubMed:18326828, ECO:0000269|PubMed:19170933, ECO:0000269|PubMed:20696912, ECO:0000269|PubMed:21917550, ECO:0000269|PubMed:22814374, ECO:0000269|PubMed:24587042}.
Function -- GeneRIF ? help Back to Top
  1. BES1 and AtMYB30 function cooperatively to promote brassinosteroid target gene expression.
    [PMID: 19170933]
  2. Results suggest that RNA silencing directly mediates downregulation of AtMYB30 expression, both in young seedlings and in adult plants.
    [PMID: 20605724]
  3. AtMYB30 and the secreted phospholipase AtsPLA(2)-alpha physically interact in vivo, following the AtMYB30-mediated specific relocalization of AtsPLA(2)-alpha from cytoplasmic vesicles to the plant cell nucleus.
    [PMID: 20696912]
  4. coordination of ABI5 and MYB30 sumoylation by SIZ1 may balance gene expression, which is required for regulation of ABA signaling during seed germination.
    [PMID: 22814374]
  5. MIEL1 (MYB30-Interacting E3 Ligase1), an Arabidopsis RING-type E3 ubiquitin ligase that interacts with and ubiquitinates MYB30, leads to MYB30 proteasomal degradation and downregulation of its transcriptional activity.
    [PMID: 23403577]
  6. S-nitrosylation influences the structure and DNA binding activity of MYB30 from Arabidopsis thaliana.
    [PMID: 24583075]
  7. Elevated levels of MYB30 in the phloem accelerate flowering in Arabidopsis through the regulation of FLOWERING LOCUS T.
    [PMID: 24587042]
  8. The SBT5.2 gene produces two distinct transcripts encoding a canonical secreted subtilase [SBT5.2(a)] and an intracellular protein [SBT5.2(b)]. SBT5.2(b) localizes to endosomes where it interacts with and retains the defence-related transcription factor MYB30.
    [PMID: 27685353]
  9. MYB30 regulates oxidative and heat stress responses through calcium signaling, which is at least partially mediated by ANN1 and ANN4.
    [PMID: 28726305]
  10. MYB30-INTERACTING E3 LIGASE 1 (MIEL1) E3 ubiquitin ligase controls the protein stability of the two MYB transcription factors, MYB96 and MYB30, and thereby wax biosynthesis in Arabidopsis. These observations indicate that the MIEL1-MYB96 module is important for balanced cuticular wax biosynthesis in developing inflorescence stems.
    [PMID: 28838126]
  11. showed that MYB30 is necessary for root growth regulation during defense responses
    [PMID: 29712840]
  12. Data show that the RING-type ubiquitin E3 ligase RHA2b, which positively regulates abscisic acid (ABA) signaling, interacted with and ubiquitinated transcription factor MYB30 to modulate MYB30 stability.
    [PMID: 30030326]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
Motif logo
Cis-element ? help Back to Top
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Up-regulated during hypersensitive response, but no expression detected during compatible interaction with pathogens (PubMed:10571865). Specifically induced in the inoculated zone 4 hours post pathogen infection (PubMed:20696912). Up-regulated by jasmonic acid and salicylic acid (PubMed:16463103, PubMed:16730712). Transcriptionally regulated by BZR2 (PubMed:19170933). {ECO:0000269|PubMed:10571865, ECO:0000269|PubMed:16463103, ECO:0000269|PubMed:16730712, ECO:0000269|PubMed:19170933, ECO:0000269|PubMed:20696912}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT1G01120(A), AT1G02205(A), AT1G04220(A), AT1G74710(A), AT2G26250(A), AT4G33790(A)
Regulation -- Hormone ? help Back to Top
Source Hormone
AHDabscisic acid, auxin, Brassinosteroid, ethylene, gibberellin, jasmonic acid, salicylic acid
Interaction ? help Back to Top
Source Intact With
IntActSearch Q9SCU7
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: Altered brassinosteroids response phenotypes (PubMed:19170933). Hypersensitivity to ABA during germination and seedling growth (PubMed:22814374). {ECO:0000269|PubMed:19170933, ECO:0000269|PubMed:22814374}.
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT3G28910
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAF2503390.0AF250339.1 Arabidopsis thaliana Myb30 (Myb30) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_189533.10.0myb domain protein 30
SwissprotQ9SCU70.0MYB30_ARATH; Transcription factor MYB30
STRINGAT3G28910.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP5171784
Publications ? help Back to Top
  1. Daniel X,Lacomme C,Morel JB,Roby D
    A novel myb oncogene homologue in Arabidopsis thaliana related to hypersensitive cell death.
    Plant J., 1999. 20(1): p. 57-66
  2. Kleinow T, et al.
    Functional identification of an Arabidopsis snf4 ortholog by screening for heterologous multicopy suppressors of snf4 deficiency in yeast.
    Plant J., 2000. 23(1): p. 115-22
  3. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
  4. Stracke R,Werber M,Weisshaar B
    The R2R3-MYB gene family in Arabidopsis thaliana.
    Curr. Opin. Plant Biol., 2001. 4(5): p. 447-56
  5. Vailleau F, et al.
    A R2R3-MYB gene, AtMYB30, acts as a positive regulator of the hypersensitive cell death program in plants in response to pathogen attack.
    Proc. Natl. Acad. Sci. U.S.A., 2002. 99(15): p. 10179-84
  6. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
  7. Yanhui C, et al.
    The MYB transcription factor superfamily of Arabidopsis: expression analysis and phylogenetic comparison with the rice MYB family.
    Plant Mol. Biol., 2006. 60(1): p. 107-24
  8. Raffaele S,Rivas S,Roby D
    An essential role for salicylic acid in AtMYB30-mediated control of the hypersensitive cell death program in Arabidopsis.
    FEBS Lett., 2006. 580(14): p. 3498-504
  9. Raffaele S, et al.
    A MYB transcription factor regulates very-long-chain fatty acid biosynthesis for activation of the hypersensitive cell death response in Arabidopsis.
    Plant Cell, 2008. 20(3): p. 752-67
  10. Li L, et al.
    Arabidopsis MYB30 is a direct target of BES1 and cooperates with BES1 to regulate brassinosteroid-induced gene expression.
    Plant J., 2009. 58(2): p. 275-86
  11. Okada S, et al.
    Reconstitution of Arabidopsis thaliana SUMO pathways in E. coli: functional evaluation of SUMO machinery proteins and mapping of SUMOylation sites by mass spectrometry.
    Plant Cell Physiol., 2009. 50(6): p. 1049-61
  12. Reina-Pinto JJ, et al.
    Misexpression of FATTY ACID ELONGATION1 in the Arabidopsis epidermis induces cell death and suggests a critical role for phospholipase A2 in this process.
    Plant Cell, 2009. 21(4): p. 1252-72
  13. Froidure S,Roby D,Rivas S
    Expression of the Arabidopsis transcription factor AtMYB30 is post-transcriptionally regulated.
    Plant Physiol. Biochem., 2010. 48(8): p. 735-9
  14. Froidure S, et al.
    AtsPLA2-alpha nuclear relocalization by the Arabidopsis transcription factor AtMYB30 leads to repression of the plant defense response.
    Proc. Natl. Acad. Sci. U.S.A., 2010. 107(34): p. 15281-6
  15. Arabidopsis Interactome Mapping Consortium
    Evidence for network evolution in an Arabidopsis interactome map.
    Science, 2011. 333(6042): p. 601-7
  16. Canonne J, et al.
    The Xanthomonas type III effector XopD targets the Arabidopsis transcription factor MYB30 to suppress plant defense.
    Plant Cell, 2011. 23(9): p. 3498-511
  17. Bruex A, et al.
    A gene regulatory network for root epidermis cell differentiation in Arabidopsis.
    PLoS Genet., 2012. 8(1): p. e1002446
  18. Canonne J, et al.
    Identification of the protein sequence of the type III effector XopD from the B100 strain of Xanthomonas campestris pv campestris.
    Plant Signal Behav, 2012. 7(2): p. 184-7
  19. Zheng Y,Schumaker KS,Guo Y
    Sumoylation of transcription factor MYB30 by the small ubiquitin-like modifier E3 ligase SIZ1 mediates abscisic acid response in Arabidopsis thaliana.
    Proc. Natl. Acad. Sci. U.S.A., 2012. 109(31): p. 12822-7
  20. Marino D, et al.
    Arabidopsis ubiquitin ligase MIEL1 mediates degradation of the transcription factor MYB30 weakening plant defence.
    Nat Commun, 2013. 4: p. 1476
  21. Li-Beisson Y, et al.
    Acyl-lipid metabolism.
    Arabidopsis Book, 2013. 11: p. e0161
  22. Raffaele S,Rivas S
    Regulate and be regulated: integration of defense and other signals by the AtMYB30 transcription factor.
    Front Plant Sci, 2013. 4: p. 98
  23. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
  24. Tavares CP, et al.
    S-nitrosylation influences the structure and DNA binding activity of AtMYB30 transcription factor from Arabidopsis thaliana.
    Biochim. Biophys. Acta, 2014. 1844(4): p. 810-7
  25. Liu L, et al.
    Elevated levels of MYB30 in the phloem accelerate flowering in Arabidopsis through the regulation of FLOWERING LOCUS T.
    PLoS ONE, 2014. 9(2): p. e89799
  26. Xie LJ, et al.
    Arabidopsis acyl-CoA-binding protein ACBP3 participates in plant response to hypoxia by modulating very-long-chain fatty acid metabolism.
    Plant J., 2015. 81(1): p. 53-67
  27. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
  28. Kaurilind E,Xu E,Brosché M
    A genetic framework for H2O2 induced cell death in Arabidopsis thaliana.
    BMC Genomics, 2015. 16: p. 837
  29. Lee HG,Seo PJ
    The Arabidopsis MIEL1 E3 ligase negatively regulates ABA signalling by promoting protein turnover of MYB96.
    Nat Commun, 2016. 7: p. 12525
  30. Serrano I, et al.
    A non canonical subtilase attenuates the transcriptional activation of defence responses in Arabidopsis thaliana.
    Elife, 2017.
  31. Liao C,Zheng Y,Guo Y
    MYB30 transcription factor regulates oxidative and heat stress responses through ANNEXIN-mediated cytosolic calcium signaling in Arabidopsis.
    New Phytol., 2017. 216(1): p. 163-177
  32. Lee HG,Kim J,Suh MC,Seo PJ
    The MIEL1 E3 Ubiquitin Ligase Negatively Regulates Cuticular Wax Biosynthesis in Arabidopsis Stems.
    Plant Cell Physiol., 2017. 58(7): p. 1249-1259
  33. Mabuchi K, et al.
    MYB30 links ROS signaling, root cell elongation, and plant immune responses.
    Proc. Natl. Acad. Sci. U.S.A., 2018. 115(20): p. E4710-E4719
  34. Zheng Y,Chen Z,Ma L,Liao C
    The Ubiquitin E3 Ligase RHA2b Promotes Degradation of MYB30 in Abscisic Acid Signaling.
    Plant Physiol., 2018. 178(1): p. 428-440