PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT3G19580.2

Common Name

AZF2, MMB12.27, MMB12.4, ZF2

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

C2H2

Protein Properties

Length: 273aa MW: 29795.7 Da PI: 8.6023

Description

zinc-finger protein 2

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT3G19580.2	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	zf-C2H2	12.1	0.00061	106	128	1	23
EEETTTTEEESSHHHHHHHHHHT CS zf-C2H2 1 ykCpdCgksFsrksnLkrHirtH 23 ykC+ C k F++ L H +H AT3G19580.2 106 YKCNVCEKAFPSYQALGGHKASH 128 9***********99999998887 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF57667	1.22E-7	105	128	No hit	No description
Gene3D	G3DSA:3.30.160.60	3.5E-5	105	128	IPR013087	Zinc finger C2H2-type/integrase DNA-binding domain
PROSITE profile	PS50157	10.887	106	133	IPR007087	Zinc finger, C2H2
SMART	SM00355	0.017	106	128	IPR015880	Zinc finger, C2H2-like
Pfam	PF13912	7.6E-13	106	129	IPR007087	Zinc finger, C2H2
PROSITE pattern	PS00028	0	108	128	IPR007087	Zinc finger, C2H2
SuperFamily	SSF57667	1.22E-7	160	187	No hit	No description
SMART	SM00355	0.17	165	187	IPR015880	Zinc finger, C2H2-like
PROSITE profile	PS50157	9.099	165	187	IPR007087	Zinc finger, C2H2
Pfam	PF13912	9.0E-14	165	189	IPR007087	Zinc finger, C2H2
PROSITE pattern	PS00028	0	167	187	IPR007087	Zinc finger, C2H2

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0009414	Biological Process	response to water deprivation
GO:0009737	Biological Process	response to abscisic acid
GO:0009738	Biological Process	abscisic acid-activated signaling pathway
GO:0009793	Biological Process	embryo development ending in seed dormancy
GO:0010200	Biological Process	response to chitin
GO:0042538	Biological Process	hyperosmotic salinity response
GO:0045892	Biological Process	negative regulation of transcription, DNA-templated
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0008270	Molecular Function	zinc ion binding
GO:0043565	Molecular Function	sequence-specific DNA binding
GO:0044212	Molecular Function	transcription regulatory region DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000025	anatomy	root tip
PO:0000037	anatomy	shoot apex
PO:0000084	anatomy	plant sperm cell
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0008019	anatomy	leaf lamina base
PO:0009005	anatomy	root
PO:0009009	anatomy	plant embryo
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0025195	anatomy	pollen tube cell
PO:0025281	anatomy	pollen
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0007064	developmental stage	LP.12 twelve leaves visible stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 273 aa Download sequence Send to blast
MALEAMNTPT SSFTRIETKE DLMNDAVFIE PWLKRKRSKR QRSHSPSSSS SSPPRSRPKS 60 QNQDLTEEEY LALCLLMLAK DQPSQTRFHQ QSQSLTPPPE SKNLPYKCNV CEKAFPSYQA 120 LGGHKASHRI KPPTVISTTA DDSTAPTISI VAGEKHPIAA SGKIHECSIC HKVFPTGQAL 180 GGHKRCHYEG NLGGGGGGGS KSISHSGSVS STVSEERSHR GFIDLNLPAL PELSLHHNPI 240 VDEEILSPLT GKKPLLLTDH DQVIKKEDLS LKI

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	32	39	LKRKRSKR

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.23023	0.0	floral meristem\| leaf\| root\| seed

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	15028256	0.0
Genevisible	257022_at	0.0
Expression Atlas	AT3G19580	-
AtGenExpress	AT3G19580	-
ATTED-II	AT3G19580	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed in roots, radicles, cotyledons, hypocotyls, leaf veins, stems, sepals, petals, stamens, placenta, funiculi and maturated seeds. {ECO:0000269\|PubMed:10806347, ECO:0000269\|PubMed:15333755, ECO:0000269\|PubMed:20619483}.

Functional Description ? help Back to Top
Source	Description
TAIR	Encodes zinc finger protein. mRNA levels are upregulated in response to ABA, high salt, and mild dessication. The protein is localized to the nucleus and acts as a transcriptional repressor.
UniProt	Transcriptional repressor involved in the inhibition of plant growth under abiotic stress conditions. Can repress the expression of various genes, including osmotic stress and abscisic acid-repressive genes and auxin-inducible genes, by binding to their promoter regions in a DNA sequence-specific manner. Acts as a negative regulator of abscisic acid (ABA) signaling during seed germination. Probably involved in jasmonate (JA) early signaling response. May regulate the expression of the JA biosynthesis gene LOX3 and control the expression of TIFY10A/JAZ1, a key repressor in the JA signaling cascade. May act as a positive regulator of leaf senescence. Has been identified as a suppressor of the deficiency of yeast snf4 mutant to grow on non-fermentable carbon source. {ECO:0000269\|PubMed:10929106, ECO:0000269\|PubMed:15333755, ECO:0000269\|PubMed:18216250, ECO:0000269\|PubMed:20619483, ECO:0000269\|PubMed:21852415, ECO:0000269\|PubMed:22709441}.

Function -- GeneRIF ? help Back to Top
Results indicate that AZF2 is a negative regulator of ABA signaling in seeds. [PMID: 20619483] AZF1 and AZF2 function as transcriptional repressors involved in the inhibition of plant growth under abiotic stress conditions. [PMID: 21852415] positive regulator of leaf senescence [PMID: 22709441] Transcript regulation of AtbZIP9 and AtAFZ2 during germination was compatible with the proposed role in transcriptional regulation of AtTrxo1. Transient over-expression of AtbZIP9 and AtAZF2 in Nicotiana benthamiana leaves demonstrated an activation effect of AtbZIP9 and a repressor effect of AtAZF2 on AtTrxo1 promoter-driven reporter expression. [PMID: 28184497]

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT3G19580.2

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By abscisic acid (ABA), ethylene, salt, cold, dehydration and the monovalent and divalent cations Li(+), Na(+), K(+), Cs(+), Ca(2+) and Mg(2+). {ECO:0000269\|PubMed:10806347, ECO:0000269\|PubMed:15333755, ECO:0000269\|PubMed:20619483, ECO:0000269\|PubMed:21852415}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Regulation -- Hormone ? help Back to Top
Source	Hormone
AHD	abscisic acid

Phenotype -- Disruption Phenotype ? help Back to Top
Source	Description
UniProt	DISRUPTION PHENOTYPE: Delayed senescence and hypersensitivity to ABA during seed germination. {ECO:0000269\|PubMed:20619483, ECO:0000269\|PubMed:22709441}.

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT3G19580

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AB030730	0.0	AB030730.1 Arabidopsis thaliana AZF2 gene for Cys2/His2-type zinc finger protein 2, complete cds.
GenBank	AF250337	0.0	AF250337.1 Arabidopsis thaliana zinc finger protein AZF2 (AZF2) mRNA, complete cds.
GenBank	AP000417	0.0	AP000417.1 Arabidopsis thaliana genomic DNA, chromosome 3, P1 clone: MMB12.
GenBank	BT003816	0.0	BT003816.1 Arabidopsis thaliana clone C105170 putative zinc finger protein (At3g19580) mRNA, complete cds.
GenBank	CP002686	0.0	CP002686.1 Arabidopsis thaliana chromosome 3, complete sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_001118663.1	0.0	zinc-finger protein 2
Refseq	NP_188592.1	0.0	zinc-finger protein 2
Swissprot	Q9SSW2	0.0	AZF2_ARATH; Zinc finger protein AZF2
TrEMBL	A0A178VJ97	0.0	A0A178VJ97_ARATH; ZF2
STRING	AT3G19580.1	0.0	(Arabidopsis thaliana)

Link Out ? help Back to Top
Phytozome	AT3G19580.2
Entrez Gene	821495
iHOP	AT3G19580
wikigenes	AT3G19580

Publications ? help Back to Top
Sakamoto H,Araki T,Meshi T,Iwabuchi M Expression of a subset of the Arabidopsis Cys(2)/His(2)-type zinc-finger protein gene family under water stress. Gene, 2000. 248(1-2): p. 23-32 [PMID:10806347] Kleinow T, et al. Functional identification of an Arabidopsis snf4 ortholog by screening for heterologous multicopy suppressors of snf4 deficiency in yeast. Plant J., 2000. 23(1): p. 115-22 [PMID:10929106] Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Jiao Y, et al. A genome-wide analysis of blue-light regulation of Arabidopsis transcription factor gene expression during seedling development. Plant Physiol., 2003. 133(4): p. 1480-93 [PMID:14605227] Guan Y,Nothnagel EA Binding of arabinogalactan proteins by Yariv phenylglycoside triggers wound-like responses in Arabidopsis cell cultures. Plant Physiol., 2004. 135(3): p. 1346-66 [PMID:15235117] Englbrecht CC,Schoof H,B Conservation, diversification and expansion of C2H2 zinc finger proteins in the Arabidopsis thaliana genome. BMC Genomics, 2004. 5(1): p. 39 [PMID:15236668] Sakamoto H, et al. Arabidopsis Cys2/His2-type zinc-finger proteins function as transcription repressors under drought, cold, and high-salinity stress conditions. Plant Physiol., 2004. 136(1): p. 2734-46 [PMID:15333755] Hampton CR, et al. Cesium toxicity in Arabidopsis. Plant Physiol., 2004. 136(3): p. 3824-37 [PMID:15489280] Suzuki N, et al. Enhanced tolerance to environmental stress in transgenic plants expressing the transcriptional coactivator multiprotein bridging factor 1c. Plant Physiol., 2005. 139(3): p. 1313-22 [PMID:16244138] Truman W,de Zabala MT,Grant M Type III effectors orchestrate a complex interplay between transcriptional networks to modify basal defence responses during pathogenesis and resistance. Plant J., 2006. 46(1): p. 14-33 [PMID:16553893] Mandaokar A, et al. Transcriptional regulators of stamen development in Arabidopsis identified by transcriptional profiling. Plant J., 2006. 46(6): p. 984-1008 [PMID:16805732] Nemhauser JL,Hong F,Chory J Different plant hormones regulate similar processes through largely nonoverlapping transcriptional responses. Cell, 2006. 126(3): p. 467-75 [PMID:16901781] Xu S,Wang X,Chen J Zinc finger protein 1 (ThZF1) from salt cress (Thellungiella halophila) is a Cys-2/His-2-type transcription factor involved in drought and salt stress. Plant Cell Rep., 2007. 26(4): p. 497-506 [PMID:17024447] Ma S,Bohnert HJ Integration of Arabidopsis thaliana stress-related transcript profiles, promoter structures, and cell-specific expression. Genome Biol., 2007. 8(4): p. R49 [PMID:17408486] Libault M,Wan J,Czechowski T,Udvardi M,Stacey G Identification of 118 Arabidopsis transcription factor and 30 ubiquitin-ligase genes responding to chitin, a plant-defense elicitor. Mol. Plant Microbe Interact., 2007. 20(8): p. 900-11 [PMID:17722694] Pauwels L, et al. Mapping methyl jasmonate-mediated transcriptional reprogramming of metabolism and cell cycle progression in cultured Arabidopsis cells. Proc. Natl. Acad. Sci. U.S.A., 2008. 105(4): p. 1380-5 [PMID:18216250] Ascencio-Ib Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection. Plant Physiol., 2008. 148(1): p. 436-54 [PMID:18650403] Wang Y, et al. Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis. Plant Physiol., 2008. 148(3): p. 1201-11 [PMID:18775970] Pauwels L,Goossens A Fine-tuning of early events in the jasmonate response. Plant Signal Behav, 2008. 3(10): p. 846-7 [PMID:20140232] Drechsel G,Raab S,Hoth S Arabidopsis zinc-finger protein 2 is a negative regulator of ABA signaling during seed germination. J. Plant Physiol., 2010. 167(16): p. 1418-21 [PMID:20619483] Kodaira KS, et al. Arabidopsis Cys2/His2 zinc-finger proteins AZF1 and AZF2 negatively regulate abscisic acid-repressive and auxin-inducible genes under abiotic stress conditions. Plant Physiol., 2011. 157(2): p. 742-56 [PMID:21852415] Gaudinier A, et al. Enhanced Y1H assays for Arabidopsis. Nat. Methods, 2011. 8(12): p. 1053-5 [PMID:22037706] Li Z,Peng J,Wen X,Guo H Gene network analysis and functional studies of senescence-associated genes reveal novel regulators of Arabidopsis leaf senescence. J Integr Plant Biol, 2012. 54(8): p. 526-39 [PMID:22709441] Schweizer F,Bodenhausen N,Lassueur S,Masclaux FG,Reymond P Differential Contribution of Transcription Factors to Arabidopsis thaliana Defense Against Spodoptera littoralis. Front Plant Sci, 2013. 4: p. 13 [PMID:23382734] Efroni I, et al. Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses. Dev. Cell, 2013. 24(4): p. 438-45 [PMID:23449474] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178] Ortiz-Espín A, et al. Mitochondrial AtTrxo1 is transcriptionally regulated by AtbZIP9 and AtAZF2 and affects seed germination under saline conditions. J. Exp. Bot., 2017. 68(5): p. 1025-1038 [PMID:28184497]