PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT3G11020.1

Common Name

DREB2, DREB2B, ERF044, F9F8.16

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

ERF

Protein Properties

Length: 330aa MW: 37117 Da PI: 4.7718

Description

DRE/CRT-binding protein 2B

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT3G11020.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	AP2	56.3	7.9e-18	76	126	1	55
AP2 1 sgykGVrwdkkrgrWvAeIrdpsengkrkrfslgkfgtaeeAakaaiaarkkleg 55 + ++GVr++ +g+WvAeIr+p++ +r +lg+f tae+Aa a+++a+ +++g AT3G11020.1 76 CSFRGVRQRI-WGKWVAEIREPKI---GTRLWLGTFPTAEKAASAYDEAATAMYG 126 579**998.******954...3********************99987 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
CDD	cd00018	7.89E-34	76	136	No hit	No description
Pfam	PF00847	3.5E-12	76	126	IPR001471	AP2/ERF domain
PROSITE profile	PS51032	21.628	77	134	IPR001471	AP2/ERF domain
SMART	SM00380	4.0E-40	77	140	IPR001471	AP2/ERF domain
Gene3D	G3DSA:3.30.730.10	1.9E-30	77	136	IPR001471	AP2/ERF domain
PRINTS	PR00367	1.5E-9	78	89	IPR001471	AP2/ERF domain
SuperFamily	SSF54171	5.56E-20	78	136	IPR016177	DNA-binding domain
PRINTS	PR00367	1.5E-9	100	116	IPR001471	AP2/ERF domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0009414	Biological Process	response to water deprivation
GO:0009555	Biological Process	pollen development
GO:0010286	Biological Process	heat acclimation
GO:0045893	Biological Process	positive regulation of transcription, DNA-templated
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding
GO:0044212	Molecular Function	transcription regulatory region DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000084	anatomy	plant sperm cell
PO:0000293	anatomy	guard cell
PO:0009005	anatomy	root
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0025195	anatomy	pollen tube cell
PO:0025281	anatomy	pollen
PO:0001016	developmental stage	L mature pollen stage
PO:0001017	developmental stage	M germinated pollen stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 330 aa Download sequence Send to blast
MAVYEQTGTE QPKKRKSRAR AGGLTVADRL KKWKEYNEIV EASAVKEGEK PKRKVPAKGS 60 KKGCMKGKGG PDNSHCSFRG VRQRIWGKWV AEIREPKIGT RLWLGTFPTA EKAASAYDEA 120 ATAMYGSLAR LNFPQSVGSE FTSTSSQSEV CTVENKAVVC GDVCVKHEDT DCESNPFSQI 180 LDVREESCGT RPDSCTVGHQ DMNSSLNYDL LLEFEQQYWG QVLQEKEKPK QEEEEIQQQQ 240 QEQQQQQLQP DLLTVADYGW PWSNDIVNDQ TSWDPNECFD INELLGDLNE PGPHQSQDQN 300 HVNSGSYDLH PLHLEPHDGH EFNGLSSLDI

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1gcc_A	1e-18	78	137	3	63	ETHYLENE RESPONSIVE ELEMENT BINDING FACTOR 1
2gcc_A	1e-18	78	139	6	68	ATERF1
3gcc_A	1e-18	78	139	6	68	ATERF1
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Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.234	0.0	seed

Expression -- Microarray ? help Back to Top
Source	ID	E-value
Genevisible	256430_at	0.0
Expression Atlas	AT3G11020	-
AtGenExpress	AT3G11020	-
ATTED-II	AT3G11020	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed preferentially in roots and stems, and at a lower level in leaves. {ECO:0000269\|PubMed:10809011}.

Functional Description ? help Back to Top
Source	Description
TAIR	encodes a member of the DREB subfamily A-2 of ERF/AP2 transcription factor family (DREB2B). The protein contains one AP2 domain. There are eight members in this subfamily including DREB2A.
UniProt	Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]CCGAC-3'. Binding to the C-repeat/DRE element mediates high salinity- and dehydration-inducible transcription. {ECO:0000269\|PubMed:11798174}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00346	DAP	27203113	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT3G11020.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By high-salt and drought stresses. {ECO:0000269\|PubMed:10809011, ECO:0000269\|PubMed:9707537}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Interaction ? help Back to Top
Source	Intact With
IntAct	Search O82133

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT3G11020

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AB007791	0.0	AB007791.1 Arabidopsis thaliana mRNA for DREB2B, complete cds.
GenBank	AB016571	0.0	AB016571.1 Arabidopsis thaliana DREB2B gene, complete cds.
GenBank	AK117363	0.0	AK117363.1 Arabidopsis thaliana At3g11020 mRNA for putative DREB2B transcription factor, complete cds, clone: RAFL16-93-F04.
GenBank	ATAC009991	0.0	AC009991.5 Arabidopsis thaliana chromosome III BAC F9F8 genomic sequence, complete sequence.
GenBank	BT008351	0.0	BT008351.1 Arabidopsis thaliana At3g11020 mRNA, complete cds.
GenBank	CP002686	0.0	CP002686.1 Arabidopsis thaliana chromosome 3, complete sequence.
GenBank	KC412253	0.0	KC412253.1 Arabidopsis thaliana DRE/CRT-binding protein 2B (DREB2B) mRNA, DREB2B-AT3G11020 allele, complete cds, alternatively spliced.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_187713.1	0.0	DRE/CRT-binding protein 2B
Swissprot	O82133	0.0	DRE2B_ARATH; Dehydration-responsive element-binding protein 2B
TrEMBL	M4VR86	0.0	M4VR86_ARATH; DRE/CRT-binding protein 2B
STRING	AT3G11020.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM2874	17	65
Representative plant	OGRP6	16	1718

Link Out ? help Back to Top
Phytozome	AT3G11020.1
Entrez Gene	820273
iHOP	AT3G11020
wikigenes	AT3G11020

Publications ? help Back to Top
Nakashima K, et al. Organization and expression of two Arabidopsis DREB2 genes encoding DRE-binding proteins involved in dehydration- and high-salinity-responsive gene expression. Plant Mol. Biol., 2000. 42(4): p. 657-65 [PMID:10809011] Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Sakuma Y, et al. DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression. Biochem. Biophys. Res. Commun., 2002. 290(3): p. 998-1009 [PMID:11798174] Seki M, et al. Functional annotation of a full-length Arabidopsis cDNA collection. Science, 2002. 296(5565): p. 141-5 [PMID:11910074] Hao D,Yamasaki K,Sarai A,Ohme-Takagi M Determinants in the sequence specific binding of two plant transcription factors, CBF1 and NtERF2, to the DRE and GCC motifs. Biochemistry, 2002. 41(13): p. 4202-8 [PMID:11914065] Boyce JM, et al. The sfr6 mutant of Arabidopsis is defective in transcriptional activation via CBF/DREB1 and DREB2 and shows sensitivity to osmotic stress. Plant J., 2003. 34(4): p. 395-406 [PMID:12753580] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Vergnolle C, et al. The cold-induced early activation of phospholipase C and D pathways determines the response of two distinct clusters of genes in Arabidopsis cell suspensions. Plant Physiol., 2005. 139(3): p. 1217-33 [PMID:16258011] Nakano T,Suzuki K,Fujimura T,Shinshi H Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol., 2006. 140(2): p. 411-32 [PMID:16407444] Egawa C, et al. Differential regulation of transcript accumulation and alternative splicing of a DREB2 homolog under abiotic stress conditions in common wheat. Genes Genet. Syst., 2006. 81(2): p. 77-91 [PMID:16755132] Lim CJ, et al. Gene expression profiles during heat acclimation in Arabidopsis thaliana suspension-culture cells. J. Plant Res., 2006. 119(4): p. 373-83 [PMID:16807682] Qin F, et al. Regulation and functional analysis of ZmDREB2A in response to drought and heat stresses in Zea mays L. Plant J., 2007. 50(1): p. 54-69 [PMID:17346263] Schramm F, et al. A cascade of transcription factor DREB2A and heat stress transcription factor HsfA3 regulates the heat stress response of Arabidopsis. Plant J., 2008. 53(2): p. 264-74 [PMID:17999647] Wang Y, et al. Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis. Plant Physiol., 2008. 148(3): p. 1201-11 [PMID:18775970] Jaspers P, et al. Unequally redundant RCD1 and SRO1 mediate stress and developmental responses and interact with transcription factors. Plant J., 2009. 60(2): p. 268-79 [PMID:19548978] Brady SM, et al. A stele-enriched gene regulatory network in the Arabidopsis root. Mol. Syst. Biol., 2011. 7: p. 459 [PMID:21245844] Reňák D,Dupl'áková N,Honys D Wide-scale screening of T-DNA lines for transcription factor genes affecting male gametophyte development in Arabidopsis. Sex. Plant Reprod., 2012. 25(1): p. 39-60 [PMID:22101548] Vainonen JP, et al. RCD1-DREB2A interaction in leaf senescence and stress responses in Arabidopsis thaliana. Biochem. J., 2012. 442(3): p. 573-81 [PMID:22150398] Zhao K, et al. Isolation and characterization of dehydration-responsive element-binding factor 2C (MsDREB2C) from Malus sieversii Roem. Plant Cell Physiol., 2013. 54(9): p. 1415-30 [PMID:23757363] Djafi N, et al. The Arabidopsis DREB2 genetic pathway is constitutively repressed by basal phosphoinositide-dependent phospholipase C coupled to diacylglycerol kinase. Front Plant Sci, 2013. 4: p. 307 [PMID:23964284] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Kim K, et al. Alleviation of salt stress by enterobacter sp. EJ01 in tomato and Arabidopsis is accompanied by up-regulation of conserved salinity responsive factors in plants. Mol. Cells, 2014. 37(2): p. 109-17 [PMID:24598995] Miyazaki Y,Abe H,Takase T,Kobayashi M,Kiyosue T Overexpression of LOV KELCH protein 2 confers dehydration tolerance and is associated with enhanced expression of dehydration-inducible genes in Arabidopsis thaliana. Plant Cell Rep., 2015. 34(5): p. 843-52 [PMID:25627253] Lee SY,Boon NJ,Webb AA,Tanaka RJ Synergistic Activation of RD29A Via Integration of Salinity Stress and Abscisic Acid in Arabidopsis thaliana. Plant Cell Physiol., 2016. 57(10): p. 2147-2160 [PMID:27497445] Liu Q, et al. Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell, 1998. 10(8): p. 1391-406 [PMID:9707537]