PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT3G10800.1
Common NameBZIP28, T7M13.12
Organism
Arabidopsis thaliana
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bZIP
Protein Properties Length: 675aa    MW: 73432.4 Da    PI: 6.4099
Description bZIP family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT3G10800.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1bZIP_129.91.2e-09192237550
                  CHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH CS
       bZIP_1   5 krerrkqkNReAArrsRqRKkaeieeLeekvkeLeaeNkaLkkele 50 
                  ++ +r ++NRe+A+ sR+RKk+++eeLe+kvk++ a   +L  ++ 
  AT3G10800.1 192 RKLIRQIRNRESAQLSRLRKKQQTEELERKVKSMNATIAELNGKIA 237
                  6789*****************************9998877776665 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:1.20.5.1707.6E-13185250No hitNo description
SMARTSM003389.3E-12187252IPR004827Basic-leucine zipper domain
PROSITE profilePS502179.634190253IPR004827Basic-leucine zipper domain
SuperFamilySSF579597.5E-10192250No hitNo description
PfamPF001702.5E-8192249IPR004827Basic-leucine zipper domain
CDDcd147043.40E-15193243No hitNo description
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006990Biological Processpositive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response
GO:0009408Biological Processresponse to heat
GO:0000139Cellular ComponentGolgi membrane
GO:0005635Cellular Componentnuclear envelope
GO:0005654Cellular Componentnucleoplasm
GO:0005783Cellular Componentendoplasmic reticulum
GO:0005789Cellular Componentendoplasmic reticulum membrane
GO:0016020Cellular Componentmembrane
GO:0016021Cellular Componentintegral component of membrane
GO:0048471Cellular Componentperinuclear region of cytoplasm
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
GO:0043565Molecular Functionsequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000084anatomyplant sperm cell
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025195anatomypollen tube cell
PO:0025281anatomypollen
PO:0001016developmental stageL mature pollen stage
PO:0001017developmental stageM germinated pollen stage
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 675 aa     Download sequence    Send to blast
MTESTSVVAP PPEIPNLNPS MFSESDLFSI PPLDPLFLSD SDPISMDAPI SDLDFLLDDE  60
NGDFADFDFS FDNSDDFFDF DLSEPAVVIP EEIGNNRSNL DSSENRSGDG GLEGRSESVH  120
SQVSSQGSKT FVSDTVDASS SPESSNHQKS SVSKRKKENG DSSGELRSCK YQKSDDKSVA  180
TNNEGDDDDD KRKLIRQIRN RESAQLSRLR KKQQTEELER KVKSMNATIA ELNGKIAYVM  240
AENVALRQQM AVASGAPPMN PYMAAPPLPY QWMPYPPYPV RGYGSQTPLV PIPKLNPKPV  300
SSCRPKKAES KKNEGKSKLK KVASISFIGI LFFVFLFGTL VPFMNVNFGG ERGSFGGLSK  360
YDGHRYYDEH KGRVLMVGDG SDVRRNSGIS EGNIHSSRIS HGERDSCGGV DYNAHPKVEG  420
RPSSLSNASD PLFASLYVPR NDGLVKIDGN LIIHSVLASE KARGLGKKNI TETVKTKEPD  480
LTIPGALSSA LAVPGVRGNA AMLPHSTALS SEGKRLHQWF HEGGSGPLMD YSMCTEVFQF  540
DIAPGAIVPS SVSSISAEHL QNVTTHGKRM KNRRILEGLP VSLVASELNI TGTQPNKDAQ  600
NKTFNGNTNK PTSSSSMVVS VLLDPREVVD SETDRVVPPN PKSLSRIFVV VLLDSVKYVT  660
YSCVLPRSGL HLVAT
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.274870.0leaf| vegetative tissue
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO1453383540.0
Genevisible258759_at0.0
Expression AtlasAT3G10800-
AtGenExpressAT3G10800-
ATTED-IIAT3G10800-
Functional Description ? help Back to Top
Source Description
TAIREncodes bZIP28, a putative membrane-tethered transcriptional factor. Up-regulated in response to heat; a bZIP28 null mutant has a heat-sensitive phenotype. bZIP28 has a similar domain structure to the mammalian ATF6 protein involved in the unfolded protein response (UPR), and shares a bZIP domain, transmembrane domain, and a canonical S1P cleavage site. The bZIP28 seems to be glycosylated in vivo. bZIP28 does not appear to be transcriptionally up-regulated by UPR-inducing tunicamycin (TM) treatment. But, the expression level of three UPR-related genes is reduced in TM-treated zip28 mutants relative to wild type seedlings. And several UPR genes are transcriptionally upregulated when an N-terminal portion of the bZIP28 protein is expressed using the 35S promoter. A myc:bZIP28 fusion protein appears to be cleaved, likely at a canonical S2 cleavage site, following a TM treatment or a DTT stress-inducing treatment, but not a salt treatment. A portion of the mGFP:bZIP28 protein present in root cells appears to translocate from the cytoplasm and ER to the nucleus following TM treatment.
UniProtTranscriptional activator involved in ER stress responses. Functions as a stress sensor and transducer in ER stress signaling pathway. After proteolysis by SBT6.1 (S1P) and S2P, the N-terminal bZIP component is translocated to the nucleus, where it activates the expression and production of ER chaperones (PubMed:18156219, PubMed:18634751, PubMed:18849477, PubMed:20876872). Following ER stress, activates proteins involved in brassinosteroid (BR) signaling, which is required for stress acclimation and growth (PubMed:20876872). {ECO:0000269|PubMed:18156219, ECO:0000269|PubMed:18634751, ECO:0000269|PubMed:18849477, ECO:0000269|PubMed:20876872}.
Function -- GeneRIF ? help Back to Top
  1. Data show that bZIP28 serves as a sensor/transducer in Arabidopsis to mediate ER stress responses related to unfolded protein response.
    [PMID: 18156219]
  2. N-terminal fragment of AtbZIP28 translocates to the nucleus in response to ER stress.
    [PMID: 18634751]
  3. bZIP28 is an essential component of a membrane-tethered transcription factor-based signaling pathway that contributes to heat tolerance
    [PMID: 18849477]
  4. In response to ER stress, bZIP28 is mobilized by proteolysis and recruits NF-Y subunits to form a transcriptional complex that upregulates the expression of ER stress-induced genes.
    [PMID: 20207753]
  5. bZIP28 is dispersed in the endoplasmic reticulum in unstressed cells, but its distribution changes upon tunicamycin treatment prior to its movement into the nuclei via Golgi bodies.
    [PMID: 22335396]
  6. The bZIP28 lumen-facing C-terminus plays important roles in the endoplasmic reticulum-to-Golgi movement of bZIP28, which may contribute to the sensing of the endoplasmic reticulum stress.
    [PMID: 23558471]
  7. Data show detectable amounts of YFP-bZIP28 escaped from endoplasmic reticulum (ER) to nuclei under unstressed conditions in the bip1/bip1 bip2/+ and in the bip1/+ bip2/bip2 and bip3 mutant lines.
    [PMID: 23624714]
  8. bZIP28 and bZIP60 interact with Ash2 and WDR5a, both of which are core COMPASS-like components.
    [PMID: 25730865]
  9. Study demonstrated the flexible mode of heat response pathways involving bZIP28, HSFA2 and ROS-dependent signals.
    [PMID: 28873003]
  10. These findings reveal a functional independence of bZIP28 and bZIP60 in plant UPR, and identify an antagonizing role of BI1 in the pro-adaptive signaling mediated by bZIP28, bringing to light the distinctive complexity of the unfolded protein response (UPR) in plants.
    [PMID: 29124827]
  11. Bzip28 is a modulator of the unfolded protein response.
    [PMID: 29367234]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00345DAP27203113Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT3G10800.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By heat shock. {ECO:0000269|PubMed:18849477}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT5G42020(A)
Interaction ? help Back to Top
Source Intact With
BioGRIDAT3G10800, AT3G56660, AT4G14540, AT1G42990, AT1G56170
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT3G10800
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAK2269280.0AK226928.1 Arabidopsis thaliana mRNA for bZIP transcription factor AtbZip28, complete cds, clone: RAFL09-25-P21.
GenBankBT0025020.0BT002502.1 Arabidopsis thaliana putative bZIP transcription factor (At3g10800) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_187691.10.0Basic-leucine zipper (bZIP) transcription factor family protein
SwissprotQ9SG860.0BZP28_ARATH; bZIP transcription factor 28
TrEMBLA0A178VDM10.0A0A178VDM1_ARATH; BZIP28
STRINGAT3G10800.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM143971822
Representative plantOGRP21711628
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Jakoby M, et al.
    bZIP transcription factors in Arabidopsis.
    Trends Plant Sci., 2002. 7(3): p. 106-11
    [PMID:11906833]
  3. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
    [PMID:14593172]
  4. Zhang S, et al.
    Resources for targeted insertional and deletional mutagenesis in Arabidopsis.
    Plant Mol. Biol., 2003. 53(1-2): p. 133-50
    [PMID:14756312]
  5. Deppmann CD, et al.
    Dimerization specificity of all 67 B-ZIP motifs in Arabidopsis thaliana: a comparison to Homo sapiens B-ZIP motifs.
    Nucleic Acids Res., 2004. 32(11): p. 3435-45
    [PMID:15226410]
  6. Deppmann CD,Alvania RS,Taparowsky EJ
    Cross-species annotation of basic leucine zipper factor interactions: Insight into the evolution of closed interaction networks.
    Mol. Biol. Evol., 2006. 23(8): p. 1480-92
    [PMID:16731568]
  7. Cheng C, et al.
    An early response regulatory cluster induced by low temperature and hydrogen peroxide in seedlings of chilling-tolerant japonica rice.
    BMC Genomics, 2007. 8: p. 175
    [PMID:17577400]
  8. Liu JX,Srivastava R,Che P,Howell SH
    Salt stress responses in Arabidopsis utilize a signal transduction pathway related to endoplasmic reticulum stress signaling.
    Plant J., 2007. 51(5): p. 897-909
    [PMID:17662035]
  9. Liu JX,Srivastava R,Che P,Howell SH
    An endoplasmic reticulum stress response in Arabidopsis is mediated by proteolytic processing and nuclear relocation of a membrane-associated transcription factor, bZIP28.
    Plant Cell, 2007. 19(12): p. 4111-9
    [PMID:18156219]
  10. Tajima H,Iwata Y,Iwano M,Takayama S,Koizumi N
    Identification of an Arabidopsis transmembrane bZIP transcription factor involved in the endoplasmic reticulum stress response.
    Biochem. Biophys. Res. Commun., 2008. 374(2): p. 242-7
    [PMID:18634751]
  11. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
    [PMID:18650403]
  12. Wang Y, et al.
    Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis.
    Plant Physiol., 2008. 148(3): p. 1201-11
    [PMID:18775970]
  13. Gao H,Brandizzi F,Benning C,Larkin RM
    A membrane-tethered transcription factor defines a branch of the heat stress response in Arabidopsis thaliana.
    Proc. Natl. Acad. Sci. U.S.A., 2008. 105(42): p. 16398-403
    [PMID:18849477]
  14. Iwata Y,Fedoroff NV,Koizumi N
    Arabidopsis bZIP60 is a proteolysis-activated transcription factor involved in the endoplasmic reticulum stress response.
    Plant Cell, 2008. 20(11): p. 3107-21
    [PMID:19017746]
  15. Liu JX,Howell SH
    bZIP28 and NF-Y transcription factors are activated by ER stress and assemble into a transcriptional complex to regulate stress response genes in Arabidopsis.
    Plant Cell, 2010. 22(3): p. 782-96
    [PMID:20207753]
  16. Hanada K, et al.
    Functional compensation of primary and secondary metabolites by duplicate genes in Arabidopsis thaliana.
    Mol. Biol. Evol., 2011. 28(1): p. 377-82
    [PMID:20736450]
  17. Che P, et al.
    Signaling from the endoplasmic reticulum activates brassinosteroid signaling and promotes acclimation to stress in Arabidopsis.
    Sci Signal, 2010. 3(141): p. ra69
    [PMID:20876872]
  18. Srivastava R,Chen Y,Deng Y,Brandizzi F,Howell SH
    Elements proximal to and within the transmembrane domain mediate the organelle-to-organelle movement of bZIP28 under ER stress conditions.
    Plant J., 2012. 70(6): p. 1033-42
    [PMID:22335396]
  19. Qiang X,Zechmann B,Reitz MU,Kogel KH,Sch
    The mutualistic fungus Piriformospora indica colonizes Arabidopsis roots by inducing an endoplasmic reticulum stress-triggered caspase-dependent cell death.
    Plant Cell, 2012. 24(2): p. 794-809
    [PMID:22337916]
  20. Iwata Y,Koizumi N
    Plant transducers of the endoplasmic reticulum unfolded protein response.
    Trends Plant Sci., 2012. 17(12): p. 720-7
    [PMID:22796463]
  21. Sun L, et al.
    The lumen-facing domain is important for the biological function and organelle-to-organelle movement of bZIP28 during ER stress in Arabidopsis.
    Mol Plant, 2013. 6(5): p. 1605-15
    [PMID:23558471]
  22. Srivastava R,Deng Y,Shah S,Rao AG,Howell SH
    BINDING PROTEIN is a master regulator of the endoplasmic reticulum stress sensor/transducer bZIP28 in Arabidopsis.
    Plant Cell, 2013. 25(4): p. 1416-29
    [PMID:23624714]
  23. Sun L, et al.
    The plant-specific transcription factor gene NAC103 is induced by bZIP60 through a new cis-regulatory element to modulate the unfolded protein response in Arabidopsis.
    Plant J., 2013. 76(2): p. 274-86
    [PMID:23869562]
  24. Deng Y,Srivastava R,Howell SH
    Protein kinase and ribonuclease domains of IRE1 confer stress tolerance, vegetative growth, and reproductive development in Arabidopsis.
    Proc. Natl. Acad. Sci. U.S.A., 2013. 110(48): p. 19633-8
    [PMID:24145452]
  25. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
    [PMID:24377444]
  26. Nagashima Y,Iwata Y,Ashida M,Mishiba K,Koizumi N
    Exogenous salicylic acid activates two signaling arms of the unfolded protein response in Arabidopsis.
    Plant Cell Physiol., 2014. 55(10): p. 1772-8
    [PMID:25138441]
  27. Sun L,Zhang SS,Lu SJ,Liu JX
    Site-1 protease cleavage site is important for the ER stress-induced activation of membrane-associated transcription factor bZIP28 in Arabidopsis.
    Sci China Life Sci, 2015. 58(3): p. 270-5
    [PMID:25634523]
  28. Song ZT, et al.
    Transcription factor interaction with COMPASS-like complex regulates histone H3K4 trimethylation for specific gene expression in plants.
    Proc. Natl. Acad. Sci. U.S.A., 2015. 112(9): p. 2900-5
    [PMID:25730865]
  29. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]
  30. Zhang L,Chen H,Brandizzi F,Verchot J,Wang A
    The UPR branch IRE1-bZIP60 in plants plays an essential role in viral infection and is complementary to the only UPR pathway in yeast.
    PLoS Genet., 2015. 11(4): p. e1005164
    [PMID:25875739]
  31. Ma ZX, et al.
    The THERMOSENSITIVE MALE STERILE 1 Interacts with the BiPs via DnaJ Domain and Stimulates Their ATPase Enzyme Activities in Arabidopsis.
    PLoS ONE, 2015. 10(7): p. e0132500
    [PMID:26186593]
  32. Sagor GH, et al.
    The polyamine spermine induces the unfolded protein response via the MAPK cascade in Arabidopsis.
    Front Plant Sci, 2015. 6: p. 687
    [PMID:26442007]
  33. Kørner CJ,Du X,Vollmer ME,Pajerowska-Mukhtar KM
    Endoplasmic Reticulum Stress Signaling in Plant Immunity--At the Crossroad of Life and Death.
    Int J Mol Sci, 2015. 16(11): p. 26582-98
    [PMID:26556351]
  34. Zeng Y, et al.
    Unique COPII component AtSar1a/AtSec23a pair is required for the distinct function of protein ER export in Arabidopsis thaliana.
    Proc. Natl. Acad. Sci. U.S.A., 2015. 112(46): p. 14360-5
    [PMID:26578783]
  35. Meng Z,Ruberti C,Gong Z,Brandizzi F
    CPR5 modulates salicylic acid and the unfolded protein response to manage tradeoffs between plant growth and stress responses.
    Plant J., 2017. 89(3): p. 486-501
    [PMID:27747970]
  36. Nawkar GM, et al.
    HY5, a positive regulator of light signaling, negatively controls the unfolded protein response in Arabidopsis.
    Proc. Natl. Acad. Sci. U.S.A., 2017. 114(8): p. 2084-2089
    [PMID:28167764]
  37. Iwata Y, et al.
    Activation of the Arabidopsis membrane-bound transcription factor bZIP28 is mediated by site-2 protease, but not site-1 protease.
    Plant J., 2017. 91(3): p. 408-415
    [PMID:28407373]
  38. Zhang SS, et al.
    Tissue-Specific Transcriptomics Reveals an Important Role of the Unfolded Protein Response in Maintaining Fertility upon Heat Stress in Arabidopsis.
    Plant Cell, 2017. 29(5): p. 1007-1023
    [PMID:28442596]
  39. Ezer D, et al.
    The G-Box Transcriptional Regulatory Code in Arabidopsis.
    Plant Physiol., 2017. 175(2): p. 628-640
    [PMID:28864470]
  40. Kataoka R,Takahashi M,Suzuki N
    Coordination between bZIP28 and HSFA2 in the regulation of heat response signals in Arabidopsis.
    Plant Signal Behav, 2017. 12(11): p. e1376159
    [PMID:28873003]
  41. Ruberti C,Lai Y,Brandizzi F
    Recovery from temporary endoplasmic reticulum stress in plants relies on the tissue-specific and largely independent roles of bZIP28 and bZIP60, as well as an antagonizing function of BAX-Inhibitor 1 upon the pro-adaptive signaling mediated by bZIP28.
    Plant J., 2018. 93(1): p. 155-165
    [PMID:29124827]
  42. Kim JS,Yamaguchi-Shinozaki K,Shinozaki K
    ER-Anchored Transcription Factors bZIP17 and bZIP28 Regulate Root Elongation.
    Plant Physiol., 2018. 176(3): p. 2221-2230
    [PMID:29367234]