PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT3G10500.1

Common Name

AIF, ANAC053, F13M14.22, F18K10.7, NAC053, NTL4

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

NAC

Protein Properties

Length: 549aa MW: 62356.1 Da PI: 4.3715

Description

NAC domain containing protein 53

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT3G10500.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	NAM	169.6	9.7e-53	9	136	1	128
NAM 1 lppGfrFhPtdeelvveyLkkkvegkkleleevikevdiykvePwdLp.k.kvkaeekewyfFskrdkkyatgkrknratksgyWkatgkdkevlskk 96 l+pGfrFhPtdeelv +yLk+k+ +k++++ ++i+ +d+yk+ePwdLp k ++k+++ ewyfFs dkky++g+++nrat+ gyWk+tgkd+e+l+ + AT3G10500.1 9 LAPGFRFHPTDEELVRYYLKRKICNKPFKF-DAISVTDVYKSEPWDLPdKsRLKSRDLEWYFFSMLDKKYRNGSKTNRATEMGYWKTTGKDREILN-G 104 579************************9.99**********96347777888**********************************.9 PP NAM 97 gelvglkktLvfykgrapkgektdWvmheyrl 128 +++vg+kktLv++kgrap+ge+t+Wvmheyrl AT3G10500.1 105 SKVVGMKKTLVYHKGRAPRGERTNWVMHEYRL 136 ****************************98 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF101941	4.05E-61	7	159	IPR003441	NAC domain
PROSITE profile	PS51005	57.856	9	159	IPR003441	NAC domain
Pfam	PF02365	1.3E-27	11	136	IPR003441	NAC domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0009819	Biological Process	drought recovery
GO:0010150	Biological Process	leaf senescence
GO:0045893	Biological Process	positive regulation of transcription, DNA-templated
GO:2000377	Biological Process	regulation of reactive oxygen species metabolic process
GO:0005634	Cellular Component	nucleus
GO:0005737	Cellular Component	cytoplasm
GO:0005789	Cellular Component	endoplasmic reticulum membrane
GO:0005886	Cellular Component	plasma membrane
GO:0016021	Cellular Component	integral component of membrane
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0008019	anatomy	leaf lamina base
PO:0009005	anatomy	root
PO:0009006	anatomy	shoot system
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0025281	anatomy	pollen
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007064	developmental stage	LP.12 twelve leaves visible stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 549 aa Download sequence Send to blast
MGRGSVTSLA PGFRFHPTDE ELVRYYLKRK ICNKPFKFDA ISVTDVYKSE PWDLPDKSRL 60 KSRDLEWYFF SMLDKKYRNG SKTNRATEMG YWKTTGKDRE ILNGSKVVGM KKTLVYHKGR 120 APRGERTNWV MHEYRLVDQD LDKTGVHQDA FVLCRIFQKS GSGPKNGEQY GAPFVEEEWE 180 EEDDMTFVPD QEDLGSEDHV YVHMDDIDQK SENFVVYDAI PIPLNFIHGE SSNNVETNYS 240 DSINYIQQTG NYMDSGGYFE QPAESYEKDQ KPIIRDRDGS LQNEGIGCGV QDKHSETLQS 300 SDNIFGTDTS CYNDFPVESN YLIGEAFLDP NSNLLENDGL YLETNDLSST QQDGFDFEDY 360 LTFFDETFDP SQLMGNEDVF FDQEELFQEV ETKELEKEET SRSKHVVEEK EKDEASCSKQ 420 VDADATEFEP DYKYPLLKKA SHMLGAIPAP LANASEFPTK DAAIRLHAAQ SSGSVHVTAG 480 MITISDSNMG WSYGKNENLD LILSLGLVQG NTAPEKSGNS SAWAMLIFMC FWVLLLSVSF 540 KVSILVSSR

Sequence ? help Back to Top

Protein Sequence Length: 549 aa Download sequence Send to blast

MGRGSVTSLA PGFRFHPTDE ELVRYYLKRK ICNKPFKFDA ISVTDVYKSE PWDLPDKSRL  60
KSRDLEWYFF SMLDKKYRNG SKTNRATEMG YWKTTGKDRE ILNGSKVVGM KKTLVYHKGR  120
APRGERTNWV MHEYRLVDQD LDKTGVHQDA FVLCRIFQKS GSGPKNGEQY GAPFVEEEWE  180
EEDDMTFVPD QEDLGSEDHV YVHMDDIDQK SENFVVYDAI PIPLNFIHGE SSNNVETNYS  240
DSINYIQQTG NYMDSGGYFE QPAESYEKDQ KPIIRDRDGS LQNEGIGCGV QDKHSETLQS  300
SDNIFGTDTS CYNDFPVESN YLIGEAFLDP NSNLLENDGL YLETNDLSST QQDGFDFEDY  360
LTFFDETFDP SQLMGNEDVF FDQEELFQEV ETKELEKEET SRSKHVVEEK EKDEASCSKQ  420
VDADATEFEP DYKYPLLKKA SHMLGAIPAP LANASEFPTK DAAIRLHAAQ SSGSVHVTAG  480
MITISDSNMG WSYGKNENLD LILSLGLVQG NTAPEKSGNS SAWAMLIFMC FWVLLLSVSF  540
KVSILVSSR

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1ut4_A	3e-45	8	165	16	171	NO APICAL MERISTEM PROTEIN
1ut4_B	3e-45	8	165	16	171	NO APICAL MERISTEM PROTEIN
1ut7_A	3e-45	8	165	16	171	NO APICAL MERISTEM PROTEIN
1ut7_B	3e-45	8	165	16	171	NO APICAL MERISTEM PROTEIN
3swm_A	3e-45	8	165	19	174	NAC domain-containing protein 19
3swm_B	3e-45	8	165	19	174	NAC domain-containing protein 19
3swm_C	3e-45	8	165	19	174	NAC domain-containing protein 19
3swm_D	3e-45	8	165	19	174	NAC domain-containing protein 19
3swp_A	3e-45	8	165	19	174	NAC domain-containing protein 19
3swp_B	3e-45	8	165	19	174	NAC domain-containing protein 19
3swp_C	3e-45	8	165	19	174	NAC domain-containing protein 19
3swp_D	3e-45	8	165	19	174	NAC domain-containing protein 19
4dul_A	3e-45	8	165	16	171	NAC domain-containing protein 19
4dul_B	3e-45	8	165	16	171	NAC domain-containing protein 19
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Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	145338344	0.0
Genevisible	258921_at	0.0
Expression Atlas	AT3G10500	-
AtGenExpress	AT3G10500	-
ATTED-II	AT3G10500	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	DEVELOPMENTAL STAGE: Expressed in the anthers and the upper parts of the stamen filaments after stage 7 to 9 of flower budding. In stage 10 flower buds, expressed in the anthers and pollen grains. Weakly expressed in mature flowers at stage 12. {ECO:0000269\|PubMed:24323506}.
Uniprot	TISSUE SPECIFICITY: Expressed in roots, rosette leaves, cauline leaves, shoot apex and stems. {ECO:0000269\|PubMed:17158162}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcriptional activator activated by proteolytic cleavage through regulated intramembrane proteolysis (RIP) (Ref.6, PubMed:24323506, PubMed:25219309). Promotes reactive oxygen species (ROS) production during drought-induced leaf senescence. In response to abscisic acid (ABA)-mediated drought stress signals, binds directly to the promoters of RBOHC and RBOHE genes, encoding ROS biosynthetic enzymes, resulting in ROS accumulation and triggering leaf senescence via programmed cell death (PCD). ROS-induced leaf senescence sustains plant survival under drought conditions (PubMed:22313226). Involved in heat stress response. Modulates PCD through a ROS-mediated positive feedback control under heat stress conditions. This may provide an adaptation strategy for plant survival under extreme heat stress conditions (PubMed:25219309). Acts as repressor in preventing anther dehiscence during stamen development by suppressing genes that participate in jasmonic acid (JA) biosynthesis, such as DAD1, AOS, AOC3, OPR3 and 4CLL5/OPCL1 (PubMed:24323506). {ECO:0000269\|PubMed:22313226, ECO:0000269\|PubMed:24323506, ECO:0000269\|PubMed:25219309, ECO:0000269\|Ref.6}.

Function -- GeneRIF ? help Back to Top
NTL4 acts as a molecular switch that couples ROS metabolism to drought-induced leaf senescence. [PMID: 22313226] The present data suggest a role for AIF in controlling anther dehiscence by suppressing the expression of jasmonic acid biosynthesis genes in Arabidopsis.[AIF] [PMID: 24323506]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00343	DAP	27203113	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT3G10500.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By abscisic acid (ABA) (PubMed:22313226, PubMed:25219309). Induced by heat shock (PubMed:25219309). Induced by cold, drought stress and methyl methanesulfonate (MMS) treatment (PubMed:17158162). {ECO:0000269\|PubMed:17158162, ECO:0000269\|PubMed:22313226, ECO:0000269\|PubMed:25219309}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Phenotype -- Disruption Phenotype ? help Back to Top
Source	Description
UniProt	DISRUPTION PHENOTYPE: Delayed leaf senescence and enhanced drought resistance (PubMed:22313226). Enhanced heat resistance (PubMed:25219309). {ECO:0000269\|PubMed:22313226, ECO:0000269\|PubMed:25219309}.

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT3G10500

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AY051003	0.0	AY051003.1 Arabidopsis thaliana unknown protein (At3g10500) mRNA, complete cds.
GenBank	AY079345	0.0	AY079345.1 Arabidopsis thaliana unknown protein (At3g10500) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_566376.1	0.0	NAC domain containing protein 53
Swissprot	Q949N0	0.0	NAC53_ARATH; NAC domain-containing protein 53
TrEMBL	A0A178VIR2	0.0	A0A178VIR2_ARATH; NTL4
STRING	AT3G10500.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM3192	27	62
Representative plant	OGRP10571	5	9

Link Out ? help Back to Top
Phytozome	AT3G10500.1
Entrez Gene	820214
iHOP	AT3G10500
wikigenes	AT3G10500

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Ooka H, et al. Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana. DNA Res., 2003. 10(6): p. 239-47 [PMID:15029955] Hampton CR, et al. Cesium toxicity in Arabidopsis. Plant Physiol., 2004. 136(3): p. 3824-37 [PMID:15489280] Baerson SR, et al. Detoxification and transcriptome response in Arabidopsis seedlings exposed to the allelochemical benzoxazolin-2(3H)-one. J. Biol. Chem., 2005. 280(23): p. 21867-81 [PMID:15824099] Ohashi-Ito K,Kubo M,Demura T,Fukuda H Class III homeodomain leucine-zipper proteins regulate xylem cell differentiation. Plant Cell Physiol., 2005. 46(10): p. 1646-56 [PMID:16081527] Davletova S,Schlauch K,Coutu J,Mittler R The zinc-finger protein Zat12 plays a central role in reactive oxygen and abiotic stress signaling in Arabidopsis. Plant Physiol., 2005. 139(2): p. 847-56 [PMID:16183833] Lee BH,Henderson DA,Zhu JK The Arabidopsis cold-responsive transcriptome and its regulation by ICE1. Plant Cell, 2005. 17(11): p. 3155-75 [PMID:16214899] Vergnolle C, et al. The cold-induced early activation of phospholipase C and D pathways determines the response of two distinct clusters of genes in Arabidopsis cell suspensions. Plant Physiol., 2005. 139(3): p. 1217-33 [PMID:16258011] Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Lindqvist C, et al. An expressed sequence tag (EST) library from developing fruits of an Hawaiian endemic mint (Stenogyne rugosa, Lamiaceae): characterization and microsatellite markers. BMC Plant Biol., 2006. 6: p. 16 [PMID:16928278] Kim SY, et al. Exploring membrane-associated NAC transcription factors in Arabidopsis: implications for membrane biology in genome regulation. Nucleic Acids Res., 2007. 35(1): p. 203-13 [PMID:17158162] Culligan KM,Robertson CE,Foreman J,Doerner P,Britt AB ATR and ATM play both distinct and additive roles in response to ionizing radiation. Plant J., 2006. 48(6): p. 947-61 [PMID:17227549] Fleury D, et al. The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth. Plant Cell, 2007. 19(2): p. 417-32 [PMID:17329565] Ascencio-Ib Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection. Plant Physiol., 2008. 148(1): p. 436-54 [PMID:18650403] Inz A subcellular localization compendium of hydrogen peroxide-induced proteins. Plant Cell Environ., 2012. 35(2): p. 308-20 [PMID:21443605] Lee S,Seo PJ,Lee HJ,Park CM A NAC transcription factor NTL4 promotes reactive oxygen species production during drought-induced leaf senescence in Arabidopsis. Plant J., 2012. 70(5): p. 831-44 [PMID:22313226] Lee S,Park CM Regulation of reactive oxygen species generation under drought conditions in Arabidopsis. Plant Signal Behav, 2012. 7(6): p. 599-601 [PMID:22580707] Shih CF,Hsu WH,Peng YJ,Yang CH The NAC-like gene ANTHER INDEHISCENCE FACTOR acts as a repressor that controls anther dehiscence by regulating genes in the jasmonate biosynthesis pathway in Arabidopsis. J. Exp. Bot., 2014. 65(2): p. 621-39 [PMID:24323506] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Lee S, et al. The Arabidopsis NAC transcription factor NTL4 participates in a positive feedback loop that induces programmed cell death under heat stress conditions. Plant Sci., 2014. 227: p. 76-83 [PMID:25219309] Vermeirssen V,De Clercq I,Van Parys T,Van Breusegem F,Van de Peer Y Arabidopsis ensemble reverse-engineered gene regulatory network discloses interconnected transcription factors in oxidative stress. Plant Cell, 2014. 26(12): p. 4656-79 [PMID:25549671] Gladman NP,Marshall RS,Lee KH,Vierstra RD The Proteasome Stress Regulon Is Controlled by a Pair of NAC Transcription Factors in Arabidopsis. Plant Cell, 2016. 28(6): p. 1279-96 [PMID:27194708] Sanz-Fernández M, et al. Screening Arabidopsis mutants in genes useful for phytoremediation. J. Hazard. Mater., 2017. 335: p. 143-151 [PMID:28441590]