PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Previous version: v3.0 v4.0

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT2G47520.1

Common Name

AtERF71, ERF071, ERF71, HRE2, T30B22.18

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

ERF

Protein Properties

Length: 171aa MW: 19346.6 Da PI: 6.8342

Description

ERF family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT2G47520.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	AP2	62	1.3e-19	50	98	3	55
AP2 3 ykGVrwdkkrgrWvAeIrdpsengkrkrfslgkfgtaeeAakaaiaarkkleg 55 y+G+r+++ +g+W+AeIrdps+ r++lg+f ta+eAa+a++ a+ k++g AT2G47520.1 50 YRGIRQRP-WGKWAAEIRDPSK---GVRVWLGTFKTADEAARAYDVAAIKIRG 98 9*****.******965...4***********************98 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF54171	3.99E-23	49	107	IPR016177	DNA-binding domain
Gene3D	G3DSA:3.30.730.10	3.6E-33	49	107	IPR001471	AP2/ERF domain
PROSITE profile	PS51032	24.869	49	106	IPR001471	AP2/ERF domain
SMART	SM00380	2.0E-42	49	112	IPR001471	AP2/ERF domain
CDD	cd00018	6.53E-35	50	108	No hit	No description
Pfam	PF00847	6.1E-13	50	98	IPR001471	AP2/ERF domain
PRINTS	PR00367	1.1E-11	50	61	IPR001471	AP2/ERF domain
PRINTS	PR00367	1.1E-11	72	88	IPR001471	AP2/ERF domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009873	Biological Process	ethylene-activated signaling pathway
GO:0034059	Biological Process	response to anoxia
GO:0005634	Cellular Component	nucleus
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000037	anatomy	shoot apex
PO:0000293	anatomy	guard cell
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009046	anatomy	flower
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0007611	developmental stage	petal differentiation and expansion stage

Sequence ? help Back to Top
Protein Sequence Length: 171 aa Download sequence Send to blast
MCGGAIISDF IWSKSESEPS QLGSVSSRKK RKPVSVSEER DGKRERKNLY RGIRQRPWGK 60 WAAEIRDPSK GVRVWLGTFK TADEAARAYD VAAIKIRGRK AKLNFPNTQV EEEADTKPGG 120 NQNELISENQ VESLSEDLMA LEDYMRFYQI PVADDQSATD IGNLWSYQDS N

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2gcc_A	2e-23	50	106	6	63	ATERF1
3gcc_A	2e-23	50	106	6	63	ATERF1
Search in ModeBase

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.37019	0.0	seed

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	145361095	0.0
Genevisible	245173_at	0.0
Expression Atlas	AT2G47520	-
AtGenExpress	AT2G47520	-
ATTED-II	AT2G47520	-

Functional Description ? help Back to Top
Source	Description
TAIR	encodes a member of the ERF (ethylene response factor) subfamily B-2 of ERF/AP2 transcription factor family. The protein contains one AP2 domain. There are 5 members in this subfamily including RAP2.2 AND RAP2.12.
UniProt	Probably acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). {ECO:0000250}.

Function -- GeneRIF ? help Back to Top
HRE1 and HRE2 encode two nuclear-localized ethylene response factors specifically up-regulated under low oxygen conditions. [PMID: 20113439] AtERF71/HRE2 might function as a transcription factor involved in the response to osmotic stress as well as hypoxia. [PMID: 21946064] AtERF71/HRE2 binds to both GCC box and DRE/CRT, transactivates expression of genes downstream via GCC box or DRE/CRT, and plays an important role in root development through regulation of root cell expansion. [PMID: 25344007] Overexpression of the ERFVII member HRE2 inhibits root bending, suggesting that primary root growth direction at hypoxic conditions is antagonistically regulated by hypoxia and hypoxia-activated ERFVIIs. [PMID: 28698356] Overexpression of HRE2 induces adventitious root (AR) elongation to the same degree as hypoxia, while ethylene overrides HRE2-induced AR elongation. [PMID: 29996004]

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT2G47520.1

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT2G47520

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AY074645	0.0	AY074645.1 Arabidopsis thaliana At2g47520/T30B22.18 mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_182274.1	1e-123	Integrase-type DNA-binding superfamily protein
Swissprot	O22259	1e-124	ERF71_ARATH; Ethylene-responsive transcription factor ERF071
TrEMBL	A0A178VVJ0	1e-117	A0A178VVJ0_ARATH; HRE2
STRING	AT2G47520.1	1e-123	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM10	28	1650
Representative plant	OGRP6	16	1718

Link Out ? help Back to Top
Phytozome	AT2G47520.1
Entrez Gene	819365
iHOP	AT2G47520
wikigenes	AT2G47520

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Dal Bosco C, et al. Inactivation of the chloroplast ATP synthase gamma subunit results in high non-photochemical fluorescence quenching and altered nuclear gene expression in Arabidopsis thaliana. J. Biol. Chem., 2004. 279(2): p. 1060-9 [PMID:14576160] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Goda H, et al. Comprehensive comparison of auxin-regulated and brassinosteroid-regulated genes in Arabidopsis. Plant Physiol., 2004. 134(4): p. 1555-73 [PMID:15047898] Liu F, et al. Global transcription profiling reveals comprehensive insights into hypoxic response in Arabidopsis. Plant Physiol., 2005. 137(3): p. 1115-29 [PMID:15734912] Gonzali S, et al. The use of microarrays to study the anaerobic response in Arabidopsis. Ann. Bot., 2005. 96(4): p. 661-8 [PMID:16033780] Branco-Price C,Kawaguchi R,Ferreira RB,Bailey-Serres J Genome-wide analysis of transcript abundance and translation in Arabidopsis seedlings subjected to oxygen deprivation. Ann. Bot., 2005. 96(4): p. 647-60 [PMID:16081496] Vanderauwera S, et al. Genome-wide analysis of hydrogen peroxide-regulated gene expression in Arabidopsis reveals a high light-induced transcriptional cluster involved in anthocyanin biosynthesis. Plant Physiol., 2005. 139(2): p. 806-21 [PMID:16183842] Nakano T,Suzuki K,Fujimura T,Shinshi H Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol., 2006. 140(2): p. 411-32 [PMID:16407444] Osuna D, et al. Temporal responses of transcripts, enzyme activities and metabolites after adding sucrose to carbon-deprived Arabidopsis seedlings. Plant J., 2007. 49(3): p. 463-91 [PMID:17217462] Gandia-Herrero F, et al. Detoxification of the explosive 2,4,6-trinitrotoluene in Arabidopsis: discovery of bifunctional O- and C-glucosyltransferases. Plant J., 2008. 56(6): p. 963-74 [PMID:18702669] Licausi F, et al. HRE1 and HRE2, two hypoxia-inducible ethylene response factors, affect anaerobic responses in Arabidopsis thaliana. Plant J., 2010. 62(2): p. 302-15 [PMID:20113439] Inz A subcellular localization compendium of hydrogen peroxide-induced proteins. Plant Cell Environ., 2012. 35(2): p. 308-20 [PMID:21443605] Hess N,Klode M,Anders M,Sauter M The hypoxia responsive transcription factor genes ERF71/HRE2 and ERF73/HRE1 of Arabidopsis are differentially regulated by ethylene. Physiol Plant, 2011. 143(1): p. 41-9 [PMID:21615413] Park HY, et al. AtERF71/HRE2 transcription factor mediates osmotic stress response as well as hypoxia response in Arabidopsis. Biochem. Biophys. Res. Commun., 2011. 414(1): p. 135-41 [PMID:21946064] Gibbs DJ, et al. Homeostatic response to hypoxia is regulated by the N-end rule pathway in plants. Nature, 2011. 479(7373): p. 415-8 [PMID:22020279] Lee SY, et al. Arabidopsis AtERF71/HRE2 functions as transcriptional activator via cis-acting GCC box or DRE/CRT element and is involved in root development through regulation of root cell expansion. Plant Cell Rep., 2015. 34(2): p. 223-31 [PMID:25344007] Gasch P, et al. Redundant ERF-VII Transcription Factors Bind to an Evolutionarily Conserved cis-Motif to Regulate Hypoxia-Responsive Gene Expression in Arabidopsis. Plant Cell, 2016. 28(1): p. 160-80 [PMID:26668304] Yao Y,Chen X,Wu AM ERF-VII members exhibit synergistic and separate roles in Arabidopsis. Plant Signal Behav, 2017. 12(6): p. e1329073 [PMID:28537474] Eysholdt-Derzsó E,Sauter M Root Bending Is Antagonistically Affected by Hypoxia and ERF-Mediated Transcription via Auxin Signaling. Plant Physiol., 2017. 175(1): p. 412-423 [PMID:28698356] Eysholdt-Derzsó E,Sauter M Hypoxia and the group VII ethylene response transcription factor HRE2 promote adventitious root elongation in Arabidopsis. Plant Biol (Stuttg), 2019. 21 Suppl 1: p. 103-108 [PMID:29996004]