PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT2G46400.1

Common Name

ATWRKY46, F11C10.9, WRKY46

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

WRKY

Protein Properties

Length: 295aa MW: 33634.7 Da PI: 5.9371

Description

WRKY DNA-binding protein 46

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT2G46400.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	89.3	3.3e-28	104	164	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-ST...T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa...gCpvkkkversaedpkvveitYegeHnhe 59 dDg++WrKYGqKe++gs++pr+YYrCt++ +C + k+v++s++dp+++e++Y g+H+++ AT2G46400.1 104 DDGHCWRKYGQKEIHGSKNPRAYYRCTHRftqDCLAVKQVQKSDTDPSLFEVKYLGNHTCN 164 8*************************9999*************************97 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	1.1E-26	90	165	IPR003657	WRKY domain
PROSITE profile	PS50811	21.023	98	161	IPR003657	WRKY domain
SuperFamily	SSF118290	4.97E-24	98	164	IPR003657	WRKY domain
SMART	SM00774	6.1E-36	103	165	IPR003657	WRKY domain
Pfam	PF03106	6.5E-23	104	163	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0010200	Biological Process	response to chitin
GO:0048527	Biological Process	lateral root development
GO:0005730	Cellular Component	nucleolus
GO:0000987	Molecular Function	core promoter proximal region sequence-specific DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0008019	anatomy	leaf lamina base
PO:0009005	anatomy	root
PO:0009006	anatomy	shoot system
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0025281	anatomy	pollen
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007064	developmental stage	LP.12 twelve leaves visible stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 295 aa Download sequence Send to blast
MMMEEKLVIN ELELGKELAN RLMNNLKHTS SVDSNKTLIS DILRIYQNAI FMLSFNQDKN 60 ILKRSLEIDG KDSKNVFKKR KVSEKNTEKV KVFVATEQEN GSIDDGHCWR KYGQKEIHGS 120 KNPRAYYRCT HRFTQDCLAV KQVQKSDTDP SLFEVKYLGN HTCNNITSPK TTTNFSVSLT 180 NTNIFEGNRV HVTEQSEDMK PTKSEEVMIS LEDLENKKNI FRTFSFSNHE IENGVWKSNL 240 FLGNFVEDLS PATSGSAITS EVLSAPAAVE NSETADSYFS SLDNIIDFGQ DWLWS

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
6ir8_A	4e-23	102	163	7	68	OsWRKY45
Search in ModeBase

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.26510	0.0	root\| seed

Expression -- Microarray ? help Back to Top
Source	ID	E-value
Genevisible	263783_at	0.0
Expression Atlas	AT2G46400	-
AtGenExpress	AT2G46400	-
ATTED-II	AT2G46400	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed in guard cells, hypocotyls, and in the vascular tissues of cotyledon and root (PubMed:24773321). Mostly expressed in roots, at lower levels in leaves and petioles, and, to a lower extent, in stems, flowers and siliques (PubMed:22325892). {ECO:0000269\|PubMed:22325892, ECO:0000269\|PubMed:24773321}.

Functional Description ? help Back to Top
Source	Description
TAIR	member of WRKY Transcription Factor; Group III
UniProt	Transcription factor involved in the regulation of osmotic stress responses and stomatal movement (PubMed:24773321). Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Positive regulator of EDS1-dependent defense against E.amylovora (PubMed:22316300). Together with WRKY70 and WRKY53, promotes resistance to P.syringae, probably by enhancing salicylic acid (SA)- dependent genes. Contributes to the suppression of jasmonic acid (MeJA)-induced expression of PDF1.2 (PubMed:22325892). Together with WRKY54 and WRKY70, promotes brassinosteroid (BR)-regulated plant growth but prevent drought response by modulating gene expression (PubMed:28576847). {ECO:0000250, ECO:0000269\|PubMed:22316300, ECO:0000269\|PubMed:22325892, ECO:0000269\|PubMed:24773321, ECO:0000269\|PubMed:28576847}.

Function -- GeneRIF ? help Back to Top
WRKY28 and WRKY46 are transcriptional activators of ICS1 and PBS3, respectively, and regulate salicylic acid synthesis upon pathogen attack. [WRKY46] [PMID: 21595875] WRKY46, WRKY70, and WRKY53 positively regulate basal resistance to Pseudomonas syringae; and that they play overlapping and synergetic roles in plant basal defense. [PMID: 22325892] WRKY46 functions in Al sensitivity through regulating the expression of ALMT1. Mutation of WRKY46 leads to increased Al resistance. [PMID: 24118304] WRKY46 plays dual roles in regulating plant responses to drought and salt stress and light-dependent stomatal opening in guard cells. [PMID: 24773321] results demonstrate that WRKY46 contributes to the feedforward inhibition of osmotic/salt stress-dependent LR inhibition via regulation of ABA signaling and auxin homeostasis [PMID: 26252246] WRKY46 plays an important role in the control of root-to-shoot iron translocation under iron deficiency condition via direct regulation of VITL1 transcript levels.WRKY46 binds to the promoter region of VITL1. [PMID: 27208259] Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors Are Involved in Brassinosteroid-Regulated Plant Growth and Drought Responses [PMID: 28576847]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00112	ampDAP	27203113	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT2G46400.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Up-regulated by drought, salt and hydrogen peroxide treatments (PubMed:24773321). Induced by salicylic acid (SA) (PubMed:22268143, PubMed:22325892). Up-regulated by E.amylovora (PubMed:22316300). Triggered by P.syringae (PubMed:22325892). {ECO:0000269\|PubMed:22268143, ECO:0000269\|PubMed:22316300, ECO:0000269\|PubMed:22325892, ECO:0000269\|PubMed:24773321}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Phenotype -- Disruption Phenotype ? help Back to Top
Source	Description
UniProt	DISRUPTION PHENOTYPE: Decreased tolerance to dehydration and salt stress. {ECO:0000269\|PubMed:24773321}.

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT2G46400

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AY046275	0.0	AY046275.1 Arabidopsis thaliana WRKY transcription factor 46 (WRKY46) mRNA, complete cds.
GenBank	AY080812	0.0	AY080812.1 Arabidopsis thaliana putative WRKY-type DNA binding protein (At2g46400) mRNA, complete cds.
GenBank	AY117210	0.0	AY117210.1 Arabidopsis thaliana putative WRKY-type DNA binding protein (At2g46400) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_182163.1	0.0	WRKY DNA-binding protein 46
Swissprot	Q9SKD9	0.0	WRK46_ARATH; Probable WRKY transcription factor 46
TrEMBL	A0A178W050	0.0	A0A178W050_ARATH; WRKY46
STRING	AT2G46400.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM4888	23	52
Representative plant	OGRP14	17	875

Link Out ? help Back to Top
Phytozome	AT2G46400.1
Entrez Gene	819248
iHOP	AT2G46400
wikigenes	AT2G46400

Publications ? help Back to Top
Eulgem T,Rushton PJ,Robatzek S,Somssich IE The WRKY superfamily of plant transcription factors. Trends Plant Sci., 2000. 5(5): p. 199-206 [PMID:10785665] Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Zhu J, et al. An Arabidopsis homeodomain transcription factor gene, HOS9, mediates cold tolerance through a CBF-independent pathway. Proc. Natl. Acad. Sci. U.S.A., 2004. 101(26): p. 9873-8 [PMID:15205481] Guan Y,Nothnagel EA Binding of arabinogalactan proteins by Yariv phenylglycoside triggers wound-like responses in Arabidopsis cell cultures. Plant Physiol., 2004. 135(3): p. 1346-66 [PMID:15235117] Hass C, et al. The response regulator 2 mediates ethylene signalling and hormone signal integration in Arabidopsis. EMBO J., 2004. 23(16): p. 3290-302 [PMID:15282545] Chang S,Pikaard CS Transcript profiling in Arabidopsis reveals complex responses to global inhibition of DNA methylation and histone deacetylation. J. Biol. Chem., 2005. 280(1): p. 796-804 [PMID:15516340] Koroleva OA,Tomlinson ML,Leader D,Shaw P,Doonan JH High-throughput protein localization in Arabidopsis using Agrobacterium-mediated transient expression of GFP-ORF fusions. Plant J., 2005. 41(1): p. 162-74 [PMID:15610358] Lee BH,Henderson DA,Zhu JK The Arabidopsis cold-responsive transcriptome and its regulation by ICE1. Plant Cell, 2005. 17(11): p. 3155-75 [PMID:16214899] Suzuki N, et al. Enhanced tolerance to environmental stress in transgenic plants expressing the transcriptional coactivator multiprotein bridging factor 1c. Plant Physiol., 2005. 139(3): p. 1313-22 [PMID:16244138] Taki N, et al. 12-oxo-phytodienoic acid triggers expression of a distinct set of genes and plays a role in wound-induced gene expression in Arabidopsis. Plant Physiol., 2005. 139(3): p. 1268-83 [PMID:16258017] Stein M, et al. Arabidopsis PEN3/PDR8, an ATP binding cassette transporter, contributes to nonhost resistance to inappropriate pathogens that enter by direct penetration. Plant Cell, 2006. 18(3): p. 731-46 [PMID:16473969] Truman W,de Zabala MT,Grant M Type III effectors orchestrate a complex interplay between transcriptional networks to modify basal defence responses during pathogenesis and resistance. Plant J., 2006. 46(1): p. 14-33 [PMID:16553893] He P, et al. Specific bacterial suppressors of MAMP signaling upstream of MAPKKK in Arabidopsis innate immunity. Cell, 2006. 125(3): p. 563-75 [PMID:16678099] Mosher RA,Durrant WE,Wang D,Song J,Dong X A comprehensive structure-function analysis of Arabidopsis SNI1 defines essential regions and transcriptional repressor activity. Plant Cell, 2006. 18(7): p. 1750-65 [PMID:16766691] Tosti N, et al. Gene expression profiles of O3-treated Arabidopsis plants. Plant Cell Environ., 2006. 29(9): p. 1686-702 [PMID:16913859] Lee DJ, et al. Genome-wide expression profiling of ARABIDOPSIS RESPONSE REGULATOR 7(ARR7) overexpression in cytokinin response. Mol. Genet. Genomics, 2007. 277(2): p. 115-37 [PMID:17061125] Qutob D, et al. Phytotoxicity and innate immune responses induced by Nep1-like proteins. Plant Cell, 2006. 18(12): p. 3721-44 [PMID:17194768] Lee KP,Kim C,Landgraf F,Apel K EXECUTER1- and EXECUTER2-dependent transfer of stress-related signals from the plastid to the nucleus of Arabidopsis thaliana. Proc. Natl. Acad. Sci. U.S.A., 2007. 104(24): p. 10270-5 [PMID:17540731] Libault M,Wan J,Czechowski T,Udvardi M,Stacey G Identification of 118 Arabidopsis transcription factor and 30 ubiquitin-ligase genes responding to chitin, a plant-defense elicitor. Mol. Plant Microbe Interact., 2007. 20(8): p. 900-11 [PMID:17722694] Chawade A,Br Putative cold acclimation pathways in Arabidopsis thaliana identified by a combined analysis of mRNA co-expression patterns, promoter motifs and transcription factors. BMC Genomics, 2007. 8: p. 304 [PMID:17764576] Ascencio-Ib Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection. Plant Physiol., 2008. 148(1): p. 436-54 [PMID:18650403] van Verk MC,Bol JF,Linthorst HJ WRKY transcription factors involved in activation of SA biosynthesis genes. BMC Plant Biol., 2011. 11: p. 89 [PMID:21595875] Gaudinier A, et al. Enhanced Y1H assays for Arabidopsis. Nat. Methods, 2011. 8(12): p. 1053-5 [PMID:22037706] Moreau M, et al. EDS1 contributes to nonhost resistance of Arabidopsis thaliana against Erwinia amylovora. Mol. Plant Microbe Interact., 2012. 25(3): p. 421-30 [PMID:22316300] Hu Y,Dong Q,Yu D Arabidopsis WRKY46 coordinates with WRKY70 and WRKY53 in basal resistance against pathogen Pseudomonas syringae. Plant Sci., 2012. 185-186: p. 288-97 [PMID:22325892] Renault H, et al. γ-Aminobutyric acid transaminase deficiency impairs central carbon metabolism and leads to cell wall defects during salt stress in Arabidopsis roots. Plant Cell Environ., 2013. 36(5): p. 1009-18 [PMID:23148892] Efroni I, et al. Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses. Dev. Cell, 2013. 24(4): p. 438-45 [PMID:23449474] Ding ZJ,Yan JY,Xu XY,Li GX,Zheng SJ WRKY46 functions as a transcriptional repressor of ALMT1, regulating aluminum-induced malate secretion in Arabidopsis. Plant J., 2013. 76(5): p. 825-35 [PMID:24118304] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Ding ZJ, et al. Transcription factor WRKY46 regulates osmotic stress responses and stomatal movement independently in Arabidopsis. Plant J., 2014. 79(1): p. 13-27 [PMID:24773321] Ding ZJ, et al. Transcription factor WRKY46 modulates the development of Arabidopsis lateral roots in osmotic/salt stress conditions via regulation of ABA signaling and auxin homeostasis. Plant J., 2015. 84(1): p. 56-69 [PMID:26252246] Sheikh AH, et al. Regulation of WRKY46 Transcription Factor Function by Mitogen-Activated Protein Kinases in Arabidopsis thaliana. Front Plant Sci, 2016. 7: p. 61 [PMID:26870073] Yan JY, et al. A WRKY Transcription Factor Regulates Fe Translocation under Fe Deficiency. Plant Physiol., 2016. 171(3): p. 2017-27 [PMID:27208259] Chen J, et al. Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors Are Involved in Brassinosteroid-Regulated Plant Growth and Drought Responses. Plant Cell, 2017. 29(6): p. 1425-1439 [PMID:28576847] Ma J, et al. Overexpression of TaWRKY146 Increases Drought Tolerance through Inducing Stomatal Closure in Arabidopsis thaliana. Front Plant Sci, 2017. 8: p. 2036 [PMID:29225611]