PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT2G46310.1
Common NameCRF5, ERF068, T3F17.4
Organism
Arabidopsis thaliana
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family ERF
Protein Properties Length: 294aa    MW: 33155.9 Da    PI: 4.845
Description cytokinin response factor 5
Gene Model
Gene Model ID Type Source Coding Sequence
AT2G46310.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1AP254.82.4e-1798148256
          AP2   2 gykGVrwdkkrgrWvAeIrdpsengkrkrfslgkfgtaeeAakaaiaarkklege 56 
                  +y+GVr+++ +g+++AeIrdps+   r+r +lg+f taeeAa  +++a+ +++g+
  AT2G46310.1  98 KYRGVRQRP-WGKFAAEIRDPSS---RTRLWLGTFATAEEAAIGYDRAAIRIKGH 148
                  8********.**********965...4************************9986 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF541711.05E-1998155IPR016177DNA-binding domain
Gene3DG3DSA:3.30.730.102.8E-2998154IPR001471AP2/ERF domain
PfamPF008475.8E-1198148IPR001471AP2/ERF domain
PROSITE profilePS5103221.60198155IPR001471AP2/ERF domain
CDDcd000182.07E-3198154No hitNo description
SMARTSM003801.3E-3498161IPR001471AP2/ERF domain
PRINTSPR003676.5E-1099110IPR001471AP2/ERF domain
PRINTSPR003676.5E-10121137IPR001471AP2/ERF domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009736Biological Processcytokinin-activated signaling pathway
GO:0009873Biological Processethylene-activated signaling pathway
GO:0042991Biological Processtranscription factor import into nucleus
GO:0048825Biological Processcotyledon development
GO:0005634Cellular Componentnucleus
GO:0005737Cellular Componentcytoplasm
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000037anatomyshoot apex
PO:0000084anatomyplant sperm cell
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0009005anatomyroot
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009031anatomysepal
PO:0009046anatomyflower
PO:0009052anatomyflower pedicel
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 294 aa     Download sequence    Send to blast
MKSRVRKSKY TVHRKITSTP FDGFPKIVKI IVTDPCATDS SSDEENDNKS VAPRVKRYVD  60
EIRFCDEDDE PKPARKAKKK SPAAAAENGG DLVKSVVKYR GVRQRPWGKF AAEIRDPSSR  120
TRLWLGTFAT AEEAAIGYDR AAIRIKGHNA QTNFLTPPPS PTTEVLPETP VIDLETVSGC  180
DSARESQISL CSPTSVLRFS HNDETEYRTE PTEEQNPFFL PDLFRSGDYF WDSEITPDPL  240
FLDEFHQSLL PNINNNNTVC DKDTNLSDSF PLGVIGDFSS WDVDEFFQDH LLDK
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
1gcc_A2e-2199154359ETHYLENE RESPONSIVE ELEMENT BINDING FACTOR 1
2gcc_A2e-2199154662ATERF1
3gcc_A2e-2199154662ATERF1
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.370250.0root
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO1453610600.0
Genevisible266606_at0.0
Expression AtlasAT2G46310-
AtGenExpressAT2G46310-
ATTED-IIAT2G46310-
Functional Description ? help Back to Top
Source Description
TAIRCRF5 encodes one of the six cytokinin response factors. It is transcriptionally upregulated in response to cytokinin. CRF5 belongs to the AP2/ERF superfamily of the transcriptional factors. CRF proteins rapidly relocalize to the nucleus in response to cytokinin. Analysis of loos-of-function mutants revealed that the CRFs function redundantly to regulate the development of embryos, cotyledons and leaves.
UniProtComponent of the cytokinin signaling pathway involved in cotyledons, leaves, and embryos development. Probably acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). {ECO:0000250, ECO:0000269|PubMed:16832061}.
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT2G46310.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By cytokinins. {ECO:0000269|PubMed:16832061}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Interaction ? help Back to Top
Source Intact With
BioGRIDAT2G46310, AT3G61630, AT4G11140, AT4G23750, AT4G27950, AT5G53290, AT1G22985, AT1G71130
IntActSearch O82339
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT2G46310
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAC0053970.0AC005397.3 Arabidopsis thaliana chromosome 2 clone T3F17 map CIC02E07, complete sequence.
GenBankAK2276610.0AK227661.1 Arabidopsis thaliana mRNA for putative AP2 domain transcription factor, complete cds, clone: RAFL14-24-M06.
GenBankAY0746380.0AY074638.1 Arabidopsis thaliana At2g46310/T3F17.4 mRNA, complete cds.
GenBankBT0031210.0BT003121.1 Arabidopsis thaliana At2g46310 mRNA, complete cds.
GenBankCP0026850.0CP002685.1 Arabidopsis thaliana chromosome 2, complete sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_182154.10.0cytokinin response factor 5
SwissprotO823390.0CRF5_ARATH; Ethylene-responsive transcription factor CRF5
TrEMBLA0A178W0Y30.0A0A178W0Y3_ARATH; CRF5
STRINGAT2G46310.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM25782571
Representative plantOGRP6161718
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Brown RL,Kazan K,McGrath KC,Maclean DJ,Manners JM
    A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis.
    Plant Physiol., 2003. 132(2): p. 1020-32
    [PMID:12805630]
  3. Rashotte AM,Carson SD,To JP,Kieber JJ
    Expression profiling of cytokinin action in Arabidopsis.
    Plant Physiol., 2003. 132(4): p. 1998-2011
    [PMID:12913156]
  4. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
    [PMID:14593172]
  5. Wang R, et al.
    Genomic analysis of the nitrate response using a nitrate reductase-null mutant of Arabidopsis.
    Plant Physiol., 2004. 136(1): p. 2512-22
    [PMID:15333754]
  6. Kiba T,Aoki K,Sakakibara H,Mizuno T
    Arabidopsis response regulator, ARR22, ectopic expression of which results in phenotypes similar to the wol cytokinin-receptor mutant.
    Plant Cell Physiol., 2004. 45(8): p. 1063-77
    [PMID:15356332]
  7. Kiba T, et al.
    Combinatorial microarray analysis revealing arabidopsis genes implicated in cytokinin responses through the His->Asp Phosphorelay circuitry.
    Plant Cell Physiol., 2005. 46(2): p. 339-55
    [PMID:15695462]
  8. Duarte JM, et al.
    Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis.
    Mol. Biol. Evol., 2006. 23(2): p. 469-78
    [PMID:16280546]
  9. Nakano T,Suzuki K,Fujimura T,Shinshi H
    Genome-wide analysis of the ERF gene family in Arabidopsis and rice.
    Plant Physiol., 2006. 140(2): p. 411-32
    [PMID:16407444]
  10. Ma S,Gong Q,Bohnert HJ
    Dissecting salt stress pathways.
    J. Exp. Bot., 2006. 57(5): p. 1097-107
    [PMID:16510518]
  11. Nakano T, et al.
    Identification of genes of the plant-specific transcription-factor families cooperatively regulated by ethylene and jasmonate in Arabidopsis thaliana.
    J. Plant Res., 2006. 119(4): p. 407-13
    [PMID:16820983]
  12. Rashotte AM, et al.
    A subset of Arabidopsis AP2 transcription factors mediates cytokinin responses in concert with a two-component pathway.
    Proc. Natl. Acad. Sci. U.S.A., 2006. 103(29): p. 11081-5
    [PMID:16832061]
  13. Lee DJ, et al.
    Genome-wide expression profiling of ARABIDOPSIS RESPONSE REGULATOR 7(ARR7) overexpression in cytokinin response.
    Mol. Genet. Genomics, 2007. 277(2): p. 115-37
    [PMID:17061125]
  14. Cutcliffe JW,Hellmann E,Heyl A,Rashotte AM
    CRFs form protein-protein interactions with each other and with members of the cytokinin signalling pathway in Arabidopsis via the CRF domain.
    J. Exp. Bot., 2011. 62(14): p. 4995-5002
    [PMID:21705390]
  15. Striberny B, et al.
    Cytokinin Response Factor 5 has transcriptional activity governed by its C-terminal domain.
    Plant Signal Behav, 2017. 12(2): p. e1276684
    [PMID:28045578]