PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT2G41240.2

Common Name

BHLH100, EN7, F13H10.21

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

bHLH

Protein Properties

Length: 241aa MW: 27088.5 Da PI: 5.9607

Description

basic helix-loop-helix protein 100

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT2G41240.2	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	54	2.9e-17	62	113	1	55
CHHHHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 1 rrrahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 ++ +hn+ Er RR++iN+ f+ Lr++lP+ +++Kls ++++ +A +YI +Lq AT2G41240.2 62 KKLNHNASERERRKKINTMFSSLRSCLPPT---NQTKLSVSATVSQALKYIPELQ 113 6789***********************9...9****************998 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
CDD	cd00083	9.37E-15	60	116	No hit	No description
PROSITE profile	PS50888	14.888	61	112	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	3.9E-16	62	129	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamily	SSF47459	5.63E-18	62	132	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	8.6E-15	62	113	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	1.2E-13	67	118	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006357	Biological Process	regulation of transcription from RNA polymerase II promoter
GO:0009414	Biological Process	response to water deprivation
GO:0010106	Biological Process	cellular response to iron ion starvation
GO:0055072	Biological Process	iron ion homeostasis
GO:0090575	Cellular Component	RNA polymerase II transcription factor complex
GO:0000977	Molecular Function	RNA polymerase II regulatory region sequence-specific DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 241 aa Download sequence Send to blast
MCALVPPLYP NFGWPCGDHS FYETDDVSNT FLDFPLPDLT VTHENVSSEN NRTLLDNPVV 60 MKKLNHNASE RERRKKINTM FSSLRSCLPP TNQTKLSVSA TVSQALKYIP ELQEQVKKLM 120 KKKEELSFQI SGQRDLVYTD QNSKSEEGVT SYASTVSSTR LSETEVMVQI SSLQTEKCSF 180 GNVLSGVEED GLVLVGASSS RSHGERLFYS MHLQIKNGQV NSEELGDRLL YLYEKCGHSF 240 T

Expression -- Microarray ? help Back to Top
Source	ID
Expression Atlas	AT2G41240
AtGenExpress	AT2G41240

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed constitutively in roots, leaves, and stems. {ECO:0000269\|PubMed:12679534}.

Functional Description ? help Back to Top
Source	Description
TAIR	Encodes a member of the basic helix-loop-helix transcription factor family protein.
UniProt	Plays a role in metal homeostasis. Confers tolerance to high zinc (Zn) and nickel (Ni). {ECO:0000269\|PubMed:18088336}.

Function -- GeneRIF ? help Back to Top
up-regulated by iron deficiency in roots and leaves [PMID: 17516080] bHLH100 and bHLH101 are key regulators of iron-deficiency responses independent of the master regulator FIT [PMID: 22984573]

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT2G41240.2

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Up regulated by iron deficiency in roots and leaves, as well as by nickel, high zinc or high copper treatments. Repressed by heat treatment, high iron, low copper and low zinc treatments. {ECO:0000269\|PubMed:12679534, ECO:0000269\|PubMed:17516080, ECO:0000269\|PubMed:18088336}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT2G41240

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AY074635	0.0	AY074635.1 Arabidopsis thaliana At2g41240/F13H10.21 mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_850349.1	1e-180	basic helix-loop-helix protein 100
Swissprot	Q9ZVB5	1e-170	BH100_ARATH; Transcription factor bHLH100
TrEMBL	A0A384LFW4	1e-168	A0A384LFW4_ARATH; BHLH100
TrEMBL	C0SV82	1e-168	C0SV82_ARATH; Uncharacterized protein At2g41240 (Fragment)
STRING	AT2G41240.1	1e-169	(Arabidopsis thaliana)

Link Out ? help Back to Top
Phytozome	AT2G41240.2
Entrez Gene	818723
iHOP	AT2G41240
wikigenes	AT2G41240

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Heim MA, et al. The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity. Mol. Biol. Evol., 2003. 20(5): p. 735-47 [PMID:12679534] Rizhsky L,Liang H,Mittler R The water-water cycle is essential for chloroplast protection in the absence of stress. J. Biol. Chem., 2003. 278(40): p. 38921-5 [PMID:12885779] Toledo-Ortiz G,Huq E,Quail PH The Arabidopsis basic/helix-loop-helix transcription factor family. Plant Cell, 2003. 15(8): p. 1749-70 [PMID:12897250] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Bailey PC, et al. Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana. Plant Cell, 2003. 15(11): p. 2497-502 [PMID:14600211] Rizhsky L,Davletova S,Liang H,Mittler R The zinc finger protein Zat12 is required for cytosolic ascorbate peroxidase 1 expression during oxidative stress in Arabidopsis. J. Biol. Chem., 2004. 279(12): p. 11736-43 [PMID:14722088] van de Mortel JE, et al. Large expression differences in genes for iron and zinc homeostasis, stress response, and lignin biosynthesis distinguish roots of Arabidopsis thaliana and the related metal hyperaccumulator Thlaspi caerulescens. Plant Physiol., 2006. 142(3): p. 1127-47 [PMID:16998091] Tran LS, et al. Co-expression of the stress-inducible zinc finger homeodomain ZFHD1 and NAC transcription factors enhances expression of the ERD1 gene in Arabidopsis. Plant J., 2007. 49(1): p. 46-63 [PMID:17233795] Vorwieger A, et al. Iron assimilation and transcription factor controlled synthesis of riboflavin in plants. Planta, 2007. 226(1): p. 147-58 [PMID:17260143] Wang HY, et al. Iron deficiency-mediated stress regulation of four subgroup Ib BHLH genes in Arabidopsis thaliana. Planta, 2007. 226(4): p. 897-908 [PMID:17516080] van de Mortel JE, et al. Expression differences for genes involved in lignin, glutathione and sulphate metabolism in response to cadmium in Arabidopsis thaliana and the related Zn/Cd-hyperaccumulator Thlaspi caerulescens. Plant Cell Environ., 2008. 31(3): p. 301-24 [PMID:18088336] Huang D,Wu W,Abrams SR,Cutler AJ The relationship of drought-related gene expression in Arabidopsis thaliana to hormonal and environmental factors. J. Exp. Bot., 2008. 59(11): p. 2991-3007 [PMID:18552355] Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Ivanov R,Brumbarova T,Bauer P Fitting into the harsh reality: regulation of iron-deficiency responses in dicotyledonous plants. Mol Plant, 2012. 5(1): p. 27-42 [PMID:21873619] Wang N, et al. Requirement and functional redundancy of Ib subgroup bHLH proteins for iron deficiency responses and uptake in Arabidopsis thaliana. Mol Plant, 2013. 6(2): p. 503-13 [PMID:22983953] Sivitz AB,Hermand V,Curie C,Vert G Arabidopsis bHLH100 and bHLH101 control iron homeostasis via a FIT-independent pathway. PLoS ONE, 2012. 7(9): p. e44843 [PMID:22984573] Maurer F,Naranjo Arcos MA,Bauer P Responses of a triple mutant defective in three iron deficiency-induced Basic Helix-Loop-Helix genes of the subgroup Ib(2) to iron deficiency and salicylic acid. PLoS ONE, 2014. 9(6): p. e99234 [PMID:24919188] Martínez-Trujillo M, et al. Chromate alters root system architecture and activates expression of genes involved in iron homeostasis and signaling in Arabidopsis thaliana. Plant Mol. Biol., 2014. 86(1-2): p. 35-50 [PMID:24928490] Van Dingenen J, et al. Strobilurins as growth-promoting compounds: how Stroby regulates Arabidopsis leaf growth. Plant Cell Environ., 2017. 40(9): p. 1748-1760 [PMID:28444690] Wang C,Yao X,Yu D,Liang G Fe-deficiency-induced expression of bHLH104 enhances Fe-deficiency tolerance of Arabidopsis thaliana. Planta, 2017. 246(3): p. 421-431 [PMID:28451750] Ezer D, et al. The G-Box Transcriptional Regulatory Code in Arabidopsis. Plant Physiol., 2017. 175(2): p. 628-640 [PMID:28864470] Kailasam S,Wang Y,Lo JC,Chang HF,Yeh KC S-Nitrosoglutathione works downstream of nitric oxide to mediate iron-deficiency signaling in Arabidopsis. Plant J., 2018. 94(1): p. 157-168 [PMID:29396986] Kurt F,Filiz E Genome-wide and comparative analysis of bHLH38, bHLH39, bHLH100 and bHLH101 genes in Arabidopsis, tomato, rice, soybean and maize: insights into iron (Fe) homeostasis. Biometals, 2018. 31(4): p. 489-504 [PMID:29546482]