PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT2G40750.1

Common Name

ATWRKY54, T7D17.7, WRKY54

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

WRKY

Protein Properties

Length: 346aa MW: 38645.3 Da PI: 5.1196

Description

WRKY DNA-binding protein 54

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT2G40750.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	82.7	3.7e-26	152	212	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-ST...T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa...gCpvkkkversaedpkvveitYegeHnhe 59 +D y+WrKYGqKe+ +++fprsY+rCt++ gC+++k+v+++++d ++++itY g H+++ AT2G40750.1 152 EDRYAWRKYGQKEILNTTFPRSYFRCTHKptqGCKATKQVQKQDQDSEMFQITYIGYHTCT 212 7*************************9999*************************96 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	1.7E-25	142	212	IPR003657	WRKY domain
SuperFamily	SSF118290	7.72E-24	145	212	IPR003657	WRKY domain
PROSITE profile	PS50811	20.266	146	214	IPR003657	WRKY domain
SMART	SM00774	4.7E-36	151	213	IPR003657	WRKY domain
Pfam	PF03106	1.8E-24	152	211	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009751	Biological Process	response to salicylic acid
GO:0031347	Biological Process	regulation of defense response
GO:1900056	Biological Process	negative regulation of leaf senescence
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0005515	Molecular Function	protein binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0008019	anatomy	leaf lamina base
PO:0009005	anatomy	root
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007064	developmental stage	LP.12 twelve leaves visible stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 346 aa Download sequence Send to blast
MDSNSNNTKS IKRKVVDQLV EGYEFATQLQ LLLSHQHSNQ YHIDETRLVS GSGSVSGGPD 60 PVDELMSKIL GSFHKTISVL DSFDPVAVSV PIAVEGSWNA SCGDDSATPV SCNGGDSGES 120 KKKRLGVGKG KRGCYTRKTR SHTRIVEAKS SEDRYAWRKY GQKEILNTTF PRSYFRCTHK 180 PTQGCKATKQ VQKQDQDSEM FQITYIGYHT CTANDQTHAK TEPFDQEIIM DSEKTLAAST 240 AQNHVNAMVQ EQENNTSSVT AIDAGMVKEE QNNNGDQSKD YYEGSSTGED LSLVWQETMM 300 FDDHQNHYYC GETSTTSHQF GFIDNDDQFS SFFDSYCADY ERTSAM

Sequence ? help Back to Top

Protein Sequence Length: 346 aa Download sequence Send to blast

MDSNSNNTKS IKRKVVDQLV EGYEFATQLQ LLLSHQHSNQ YHIDETRLVS GSGSVSGGPD  60
PVDELMSKIL GSFHKTISVL DSFDPVAVSV PIAVEGSWNA SCGDDSATPV SCNGGDSGES  120
KKKRLGVGKG KRGCYTRKTR SHTRIVEAKS SEDRYAWRKY GQKEILNTTF PRSYFRCTHK  180
PTQGCKATKQ VQKQDQDSEM FQITYIGYHT CTANDQTHAK TEPFDQEIIM DSEKTLAAST  240
AQNHVNAMVQ EQENNTSSVT AIDAGMVKEE QNNNGDQSKD YYEGSSTGED LSLVWQETMM  300
FDDHQNHYYC GETSTTSHQF GFIDNDDQFS SFFDSYCADY ERTSAM

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
5w3x_B	2e-18	146	209	11	74	Disease resistance protein RRS1
5w3x_D	2e-18	146	209	11	74	Disease resistance protein RRS1
6ir8_A	2e-18	146	211	3	68	OsWRKY45
Search in ModeBase

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	42569811	0.0
Genevisible	257382_at	0.0
Expression Atlas	AT2G40750	-
AtGenExpress	AT2G40750	-
ATTED-II	AT2G40750	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	DEVELOPMENTAL STAGE: In leaves, level increases gradually up to the point of leaf senescence. {ECO:0000269\|PubMed:22268143}.
Uniprot	TISSUE SPECIFICITY: Expressed in leaves. {ECO:0000269\|PubMed:22268143}.

Functional Description ? help Back to Top
Source	Description
TAIR	member of WRKY Transcription Factor; Group III
UniProt	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Together with WRKY70, negative regulator of developmental senescence, probably via the regulation of several senescence-associated markers genes (PubMed:22268143). Positive regulator of EDS1-dependent defense against E.amylovora (PubMed:22316300). In collaboration with WRKY70, prevents stomatal closure and, consequently, osmotic stress tolerance (PubMed:23815736). Together with WRKY46 and WRKY70, promotes brassinosteroid (BR)-regulated plant growth but prevent drought response by modulating gene expression (PubMed:28576847). Negative regulator of SA biosynthesis (PubMed:28837631). Prevents defense response to the necrotrophic pathogens P.carotovorum and B.cinerea, but promotes defense against biotrophic/hemibiotrophic pathogens P.syringae pv. tomato (Pst) DC3000, probably by regulating negatively the jasmonic acid (JA)/ethylene (ET) and positively the salicylic acid (SA) signaling pathways (PubMed:28837631). {ECO:0000250\|UniProtKB:Q9SUP6, ECO:0000269\|PubMed:22268143, ECO:0000269\|PubMed:22316300, ECO:0000269\|PubMed:23815736, ECO:0000269\|PubMed:28576847, ECO:0000269\|PubMed:28837631, ECO:0000303\|PubMed:28837631}.

Function -- GeneRIF ? help Back to Top
WRKY54 and WRKY70 are negative regulators of leaf senescence. [PMID: 22268143] WRKY70 and WRKY54 co-operate as negative regulators of stomatal closure and, consequently, osmotic stress tolerance in Arabidopsis, suggesting that they have an important role, not only in plant defense, but also in abiotic stress signaling. [PMID: 23815736] Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors Are Involved in Brassinosteroid-Regulated Plant Growth and Drought Responses [PMID: 28576847] data suggest that the elevated SA level in the wrky54wrky70 double mutant results in moderate accumulation of H2O2, in promoting cell wall fortification and consequently enhanced resistance to necrotrophs but is not sufficient to trigger hypersensitive reaction (HR)-like cell death and resistance to biotrophs/hemibiotrophs like Pst DC3000 [PMID: 28837631]

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT2G40750.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced by reactive oxygen species (ROS) and salicylic acid (SA) (PubMed:22268143). Early but transient accumulation after osmotic stress (e.g. polyethylene glycol, PEG) (PubMed:23815736). Up-regulated by E.amylovora (PubMed:22316300). {ECO:0000269\|PubMed:22268143, ECO:0000269\|PubMed:22316300, ECO:0000269\|PubMed:23815736}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source	Upstream Regulator (A: Activate/R: Repress)
ATRM	AT2G24570 (R), AT4G31550 (R)

Regulation -- Hormone ? help Back to Top
Source	Hormone
AHD	salicylic acid

Interaction ? help Back to Top
Source	Intact With
BioGRID	AT5G24110

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT2G40750

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AF426253	0.0	AF426253.1 Arabidopsis thaliana WRKY transcription factor 54 (WRKY54) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_181607.1	0.0	WRKY DNA-binding protein 54
Swissprot	Q93WU8	0.0	WRK54_ARATH; Probable WRKY transcription factor 54
TrEMBL	A0A178VT46	0.0	A0A178VT46_ARATH; WRKY54
STRING	AT2G40750.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM11362	16	31
Representative plant	OGRP14	17	875

Link Out ? help Back to Top
Phytozome	AT2G40750.1
Entrez Gene	818670
iHOP	AT2G40750
wikigenes	AT2G40750

Publications ? help Back to Top
Eulgem T,Rushton PJ,Robatzek S,Somssich IE The WRKY superfamily of plant transcription factors. Trends Plant Sci., 2000. 5(5): p. 199-206 [PMID:10785665] Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] S ABA activates ADPR cyclase and cADPR induces a subset of ABA-responsive genes in Arabidopsis. Plant J., 2004. 38(3): p. 381-95 [PMID:15086800] Scheible WR, et al. Genome-wide reprogramming of primary and secondary metabolism, protein synthesis, cellular growth processes, and the regulatory infrastructure of Arabidopsis in response to nitrogen. Plant Physiol., 2004. 136(1): p. 2483-99 [PMID:15375205] Wang Y,Joshi T,Zhang XS,Xu D,Chen L Inferring gene regulatory networks from multiple microarray datasets. Bioinformatics, 2006. 22(19): p. 2413-20 [PMID:16864593] Wang D,Amornsiripanitch N,Dong X A genomic approach to identify regulatory nodes in the transcriptional network of systemic acquired resistance in plants. PLoS Pathog., 2006. 2(11): p. e123 [PMID:17096590] Journot-Catalino N,Somssich IE,Roby D,Kroj T The transcription factors WRKY11 and WRKY17 act as negative regulators of basal resistance in Arabidopsis thaliana. Plant Cell, 2006. 18(11): p. 3289-302 [PMID:17114354] Xin Z,Mandaokar A,Chen J,Last RL,Browse J Arabidopsis ESK1 encodes a novel regulator of freezing tolerance. Plant J., 2007. 49(5): p. 786-99 [PMID:17316173] Krinke O, et al. Phosphatidylinositol 4-kinase activation is an early response to salicylic acid in Arabidopsis suspension cells. Plant Physiol., 2007. 144(3): p. 1347-59 [PMID:17496105] Che P,Lall S,Howell SH Developmental steps in acquiring competence for shoot development in Arabidopsis tissue culture. Planta, 2007. 226(5): p. 1183-94 [PMID:17581762] Ascencio-Ib Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection. Plant Physiol., 2008. 148(1): p. 436-54 [PMID:18650403] Charron JB,Ouellet F,Houde M,Sarhan F The plant Apolipoprotein D ortholog protects Arabidopsis against oxidative stress. BMC Plant Biol., 2008. 8: p. 86 [PMID:18671872] Cysteine homeostasis plays an essential role in plant immunity. New Phytol., 2012. 193(1): p. 165-77 [PMID:21988475] Besseau S,Li J,Palva ET WRKY54 and WRKY70 co-operate as negative regulators of leaf senescence in Arabidopsis thaliana. J. Exp. Bot., 2012. 63(7): p. 2667-79 [PMID:22268143] Moreau M, et al. EDS1 contributes to nonhost resistance of Arabidopsis thaliana against Erwinia amylovora. Mol. Plant Microbe Interact., 2012. 25(3): p. 421-30 [PMID:22316300] Li J, et al. Defense-related transcription factors WRKY70 and WRKY54 modulate osmotic stress tolerance by regulating stomatal aperture in Arabidopsis. New Phytol., 2013. 200(2): p. 457-72 [PMID:23815736] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178] Lee MH,Jeon HS,Kim HG,Park OK An Arabidopsis NAC transcription factor NAC4 promotes pathogen-induced cell death under negative regulation by microRNA164. New Phytol., 2017. 214(1): p. 343-360 [PMID:28032643] Timmermann T, et al. Paraburkholderia phytofirmans PsJN Protects Arabidopsis thaliana Against a Virulent Strain of Pseudomonas syringae Through the Activation of Induced Resistance. Mol. Plant Microbe Interact., 2017. 30(3): p. 215-230 [PMID:28118091] Chen J, et al. Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors Are Involved in Brassinosteroid-Regulated Plant Growth and Drought Responses. Plant Cell, 2017. 29(6): p. 1425-1439 [PMID:28576847] Li J,Zhong R,Palva ET WRKY70 and its homolog WRKY54 negatively modulate the cell wall-associated defenses to necrotrophic pathogens in Arabidopsis. PLoS ONE, 2017. 12(8): p. e0183731 [PMID:28837631]