PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT2G40340.1

Common Name

AtERF48, DREB2C, ERF048, T07M07.24, T3G21.11

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

ERF

Protein Properties

Length: 341aa MW: 37829.6 Da PI: 4.4098

Description

ERF family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT2G40340.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	AP2	55.4	1.6e-17	70	120	1	55
AP2 1 sgykGVrwdkkrgrWvAeIrdpsengkrkrfslgkfgtaeeAakaaiaarkkleg 55 ++y+GVr+++ +g+WvAeIr+p g +r +lg+f + +eAa a+++a+k+++g AT2G40340.1 70 CDYRGVRQRR-WGKWVAEIREPD--G-GARLWLGTFSSSYEAALAYDEAAKAIYG 120 68***998.******83..3.4*********************9987 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF00847	1.4E-13	70	120	IPR001471	AP2/ERF domain
CDD	cd00018	6.56E-32	70	129	No hit	No description
SMART	SM00380	2.5E-37	71	134	IPR001471	AP2/ERF domain
PROSITE profile	PS51032	20.837	71	128	IPR001471	AP2/ERF domain
SuperFamily	SSF54171	9.15E-19	72	128	IPR016177	DNA-binding domain
PRINTS	PR00367	7.5E-9	72	83	IPR001471	AP2/ERF domain
Gene3D	G3DSA:3.30.730.10	1.2E-27	72	129	IPR001471	AP2/ERF domain
PRINTS	PR00367	7.5E-9	94	110	IPR001471	AP2/ERF domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0009737	Biological Process	response to abscisic acid
GO:0009738	Biological Process	abscisic acid-activated signaling pathway
GO:0010286	Biological Process	heat acclimation
GO:0045893	Biological Process	positive regulation of transcription, DNA-templated
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0005515	Molecular Function	protein binding
GO:0043565	Molecular Function	sequence-specific DNA binding
GO:0044212	Molecular Function	transcription regulatory region DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000293	anatomy	guard cell
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem

Sequence ? help Back to Top
Protein Sequence Length: 341 aa Download sequence Send to blast
MPSEIVDRKR KSRGTRDVAE ILRQWREYNE QIEAESCIDG GGPKSIRKPP PKGSRKGCMK 60 GKGGPENGIC DYRGVRQRRW GKWVAEIREP DGGARLWLGT FSSSYEAALA YDEAAKAIYG 120 QSARLNLPEI TNRSSSTAAT ATVSGSVTAF SDESEVCARE DTNASSGFGQ VKLEDCSDEY 180 VLLDSSQCIK EELKGKEEVR EEHNLAVGFG IGQDSKRETL DAWLMGNGNE QEPLEFGVDE 240 TFDINELLGI LNDNNVSGQE TMQYQVDRHP NFSYQTQFPN SNLLGSLNPM EIAQPGVDYG 300 CPYVQPSDME NYGIDLDHRR FNDLDIQDLD FGGDKDVHGS T

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1gcc_A	3e-16	72	128	3	60	ETHYLENE RESPONSIVE ELEMENT BINDING FACTOR 1
Search in ModeBase

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.37068	0.0	flower

Expression -- Microarray ? help Back to Top
Source	ID	E-value
Genevisible	263823_s_at	1e-143
Expression Atlas	AT2G40340	-
AtGenExpress	AT2G40340	-

Functional Description ? help Back to Top
Source	Description
TAIR	Encodes a member of the DREB subfamily A-2 of ERF/AP2 transcription factor family. The protein contains one AP2 domain. There are eight members in this subfamily including DREB2A AND DREB2B that are involved in response to drought.
UniProt	Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]CCGAC-3'. Binding to the C-repeat/DRE element mediates high salinity- and abscisic acid-inducible transcription. {ECO:0000269\|PubMed:11798174}.

Function -- GeneRIF ? help Back to Top
Data suggest that DREB2C is a regulator of heat stress tolerance in Arabidopsis. [PMID: 17716623] identify possible targets of DREB2C transcription factors at the protein level [PMID: 19830397] Overexpression of DREB2C affects the expression of stress-responsive genes and affects ABA sensitivity. [PMID: 20395451] these results indicate that DREB2C and HsfA3 are key players in regulating the heat tolerance of Arabidopsis. [PMID: 20849812] These results suggest that DREB2C plays an important role in promoting oxidative stress tolerance in Arabidopsis. [PMID: 22286229] The TATA-proximal region and some latent trans-acting factors may cooperate in heat shock-induced activation of the Arabidopsis DREB2C promoter. [PMID: 22716647] We propose that AtCYS4 functions in thermotolerance under the control of the DREB2C cascade [PMID: 23868510] DREB2C functions as transcriptional activator that promotes NaCl tolerance, in part through upregulation of the stress-responsive gene COR15A. [PMID: 24737413] results suggest that DREB2C is a stress- and ABA-inducible gene that acts as a positive regulator of ABA biosynthesis in germinating seeds through activating NCED9 expression [PMID: 25150152] These results demonstrate that VOZ1 functions as a negative regulator of heat stress (HS)-inducible DREB2C signaling by blocking access to the AP2 DNA-binding domain of DREB2C. [PMID: 29536220]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00302	DAP	27203113	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT2G40340.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By high-salt stress and abscisic acid (ABA) treatment. {ECO:0000269\|PubMed:11798174}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- Hormone ? help Back to Top
Source	Hormone
AHD	abscisic acid

Interaction ? help Back to Top
Source	Intact With
BioGRID	AT3G19290, AT4G34000, AT1G45249
IntAct	Search Q8LFR2

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT2G40340

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	BT026505	0.0	BT026505.1 Arabidopsis thaliana At2g40340 mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_565929.1	0.0	Integrase-type DNA-binding superfamily protein
Swissprot	Q8LFR2	0.0	DRE2C_ARATH; Dehydration-responsive element-binding protein 2C
TrEMBL	A0A178VWP8	0.0	A0A178VWP8_ARATH; DREB2C
STRING	AT2G40340.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM8532	25	38
Representative plant	OGRP6	16	1718

Link Out ? help Back to Top
Phytozome	AT2G40340.1
Entrez Gene	818627
iHOP	AT2G40340
wikigenes	AT2G40340

Publications ? help Back to Top
Wang ML, et al. A cluster of ABA-regulated genes on Arabidopsis thaliana BAC T07M07. Genome Res., 1999. 9(4): p. 325-33 [PMID:10207155] Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Sakuma Y, et al. DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression. Biochem. Biophys. Res. Commun., 2002. 290(3): p. 998-1009 [PMID:11798174] Brown RL,Kazan K,McGrath KC,Maclean DJ,Manners JM A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis. Plant Physiol., 2003. 132(2): p. 1020-32 [PMID:12805630] Nakano T,Suzuki K,Fujimura T,Shinshi H Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol., 2006. 140(2): p. 411-32 [PMID:16407444] Lim CJ, et al. Gene expression profiles during heat acclimation in Arabidopsis thaliana suspension-culture cells. J. Plant Res., 2006. 119(4): p. 373-83 [PMID:16807682] Lim CJ, et al. Over-expression of the Arabidopsis DRE/CRT-binding transcription factor DREB2C enhances thermotolerance. Biochem. Biophys. Res. Commun., 2007. 362(2): p. 431-6 [PMID:17716623] Ramel F, et al. Genome-wide interacting effects of sucrose and herbicide-mediated stress in Arabidopsis thaliana: novel insights into atrazine toxicity and sucrose-induced tolerance. BMC Genomics, 2007. 8: p. 450 [PMID:18053238] Welling A,Palva ET Involvement of CBF transcription factors in winter hardiness in birch. Plant Physiol., 2008. 147(3): p. 1199-211 [PMID:18467468] Jaspers P, et al. Unequally redundant RCD1 and SRO1 mediate stress and developmental responses and interact with transcription factors. Plant J., 2009. 60(2): p. 268-79 [PMID:19548978] Lee K, et al. Identification of potential DREB2C targets in Arabidopsis thaliana plants overexpressing DREB2C using proteomic analysis. Mol. Cells, 2009. 28(4): p. 383-8 [PMID:19830397] Lee SJ, et al. DREB2C interacts with ABF2, a bZIP protein regulating abscisic acid-responsive gene expression, and its overexpression affects abscisic acid sensitivity. Plant Physiol., 2010. 153(2): p. 716-27 [PMID:20395451] Chen H, et al. Arabidopsis DREB2C functions as a transcriptional activator of HsfA3 during the heat stress response. Biochem. Biophys. Res. Commun., 2010. 401(2): p. 238-44 [PMID:20849812] Vainonen JP, et al. RCD1-DREB2A interaction in leaf senescence and stress responses in Arabidopsis thaliana. Biochem. J., 2012. 442(3): p. 573-81 [PMID:22150398] Hwang JE, et al. Overexpression of Arabidopsis dehydration- responsive element-binding protein 2C confers tolerance to oxidative stress. Mol. Cells, 2012. 33(2): p. 135-40 [PMID:22286229] Chen H,Je J,Song C,Hwang JE,Lim CO A proximal promoter region of Arabidopsis DREB2C confers tissue-specific expression under heat stress. J Integr Plant Biol, 2012. 54(9): p. 640-51 [PMID:22716647] Je J,Song C,Hwang JE,Chung WS,Lim CO DREB2C acts as a transcriptional activator of the thermo tolerance-related phytocystatin 4 (AtCYS4) gene. Transgenic Res., 2014. 23(1): p. 109-23 [PMID:23868510] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Song C,Je J,Hong JK,Lim CO Ectopic expression of an Arabidopsis dehydration-responsive element-binding factor DREB2C improves salt stress tolerance in crucifers. Plant Cell Rep., 2014. 33(8): p. 1239-54 [PMID:24737413] Je J,Chen H,Song C,Lim CO Arabidopsis DREB2C modulates ABA biosynthesis during germination. Biochem. Biophys. Res. Commun., 2014. 452(1): p. 91-8 [PMID:25150152] Sazegari S,Niazi A,Ahmadi FS A study on the regulatory network with promoter analysis for Arabidopsis DREB-genes. Bioinformation, 2015. 11(2): p. 101-6 [PMID:25848171] Huang KC,Lin WC,Cheng WH Salt hypersensitive mutant 9, a nucleolar APUM23 protein, is essential for salt sensitivity in association with the ABA signaling pathway in Arabidopsis. BMC Plant Biol., 2018. 18(1): p. 40 [PMID:29490615] Song C,Lee J,Kim T,Hong JC,Lim CO VOZ1, a transcriptional repressor of DREB2C, mediates heat stress responses in Arabidopsis. Planta, 2018. 247(6): p. 1439-1448 [PMID:29536220]