PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT2G37260.1

Common Name

ATWRKY44, DSL1, F3G5.5, TTG2, WRKY44

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

WRKY

Protein Properties

Length: 429aa MW: 47141.2 Da PI: 9.3989

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT2G37260.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	100.8	8e-32	166	222	3	60
-SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS-- CS WRKY 3 DgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhek 60 DgynWrKYGqK+vkgse+prsYY+Ct+++Cpvkkkvers +++v ei+Y+geHnh+k AT2G37260.1 166 DGYNWRKYGQKQVKGSECPRSYYKCTHPKCPVKKKVERSV-EGQVSEIVYQGEHNHSK 222 9*************************************.*************85 PP
2	WRKY	103	1.7e-32	349	406	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 +Dg++WrKYGqK+v g+ +prsYYrCtsa+C+++k+ver+++dp+++++tYeg+Hnh+ AT2G37260.1 349 EDGFRWRKYGQKVVGGNAYPRSYYRCTSANCRARKHVERASDDPRAFITTYEGKHNHH 406 8********************************************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	5.1E-28	158	223	IPR003657	WRKY domain
SMART	SM00774	1.5E-33	164	222	IPR003657	WRKY domain
SuperFamily	SSF118290	2.22E-24	165	223	IPR003657	WRKY domain
Pfam	PF03106	1.2E-24	166	220	IPR003657	WRKY domain
PROSITE profile	PS50811	22.768	166	223	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	6.4E-32	338	406	IPR003657	WRKY domain
PROSITE profile	PS50811	31.651	343	408	IPR003657	WRKY domain
SuperFamily	SSF118290	3.53E-27	344	406	IPR003657	WRKY domain
SMART	SM00774	3.9E-38	348	407	IPR003657	WRKY domain
Pfam	PF03106	7.0E-26	349	406	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009957	Biological Process	epidermal cell fate specification
GO:0010214	Biological Process	seed coat development
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000037	anatomy	shoot apex
PO:0000263	anatomy	non-hair root epidermal cell
PO:0000293	anatomy	guard cell
PO:0006504	anatomy	leaf trichome
PO:0009005	anatomy	root
PO:0009006	anatomy	shoot system
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007611	developmental stage	petal differentiation and expansion stage

Sequence ? help Back to Top
Protein Sequence Length: 429 aa Download sequence Send to blast
MEVNDGERVV IAKPVASRPS SSSGFRTFTE LLTDSVTVSP QTTCHEIVDA AIRPKTLRFN 60 QPVAASVSCP RAEVKGIGNG MSCDDDSDSR NYVVYKPKAK LVSKATVSAL ANMLQGNRQQ 120 TWRQSEAVSY GKSVSQGTHR AGPNLVQKVP SFTESETSTG DRSSVDGYNW RKYGQKQVKG 180 SECPRSYYKC THPKCPVKKK VERSVEGQVS EIVYQGEHNH SKPSCPLPRR ASSSISSGFQ 240 KPPKSIASEG SMGQDPNNNL YSPLWNNQSN DSTQNRTEKM SEGCVITPFE FAVPRSTNSN 300 PGTSDSGCKS SQCDEGELDD PSRSKRRKNE KQSSEAGVSQ GSVESDSLED GFRWRKYGQK 360 VVGGNAYPRS YYRCTSANCR ARKHVERASD DPRAFITTYE GKHNHHLLLS PPSSSTLPFN 420 SPQLSKQTI

Sequence ? help Back to Top

Protein Sequence Length: 429 aa Download sequence Send to blast

MEVNDGERVV IAKPVASRPS SSSGFRTFTE LLTDSVTVSP QTTCHEIVDA AIRPKTLRFN  60
QPVAASVSCP RAEVKGIGNG MSCDDDSDSR NYVVYKPKAK LVSKATVSAL ANMLQGNRQQ  120
TWRQSEAVSY GKSVSQGTHR AGPNLVQKVP SFTESETSTG DRSSVDGYNW RKYGQKQVKG  180
SECPRSYYKC THPKCPVKKK VERSVEGQVS EIVYQGEHNH SKPSCPLPRR ASSSISSGFQ  240
KPPKSIASEG SMGQDPNNNL YSPLWNNQSN DSTQNRTEKM SEGCVITPFE FAVPRSTNSN  300
PGTSDSGCKS SQCDEGELDD PSRSKRRKNE KQSSEAGVSQ GSVESDSLED GFRWRKYGQK  360
VVGGNAYPRS YYRCTSANCR ARKHVERASD DPRAFITTYE GKHNHHLLLS PPSSSTLPFN  420
SPQLSKQTI

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wj2_A	3e-28	166	407	3	78	Probable WRKY transcription factor 4
2lex_A	3e-28	166	407	3	78	Probable WRKY transcription factor 4
Search in ModeBase

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.26513	0.0	flower

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	186506098	0.0
Genevisible	265954_at	0.0
Expression Atlas	AT2G37260	-
AtGenExpress	AT2G37260	-
ATTED-II	AT2G37260	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Leaf promordia, trichomes, atrichoblasts, fertilized eggs, seed coat.

Functional Description ? help Back to Top
Source	Description
TAIR	Encodes a protein similar to WRKY transcription factors that is expressed in the seed integument and endosperm. Mutants are defective in proanthocyanidin synthesis and seed mucilate deposition. Seeds are yellow colored. Seed size is also affected; seeds are reduced in size but only when the mutant allele is transmitted through the female parent.Loss of function alleles are associated with a reduction in interploidy lethality.
UniProt	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Regulates trichome development, production of mucilage and tannin in seed coats, and maybe root hair development. {ECO:0000269\|PubMed:12084832}.

Function -- GeneRIF ? help Back to Top
Complexes containing R2R3 MYB and bHLH transcription factors regulate the expression of TTG2, which then regulates GL2 expression with complexes containing R2R3 MYB. [PMID: 17766401] the role of the sporophytically active TTG2 gene in interploidy crosses indicates that the developmental programming of the mother regulates the viability of interploidy hybrid offspring. [PMID: 19071961] GIGANTEA-miRNA172-WRKY44 may regulate drought escape and drought tolerance by affecting sugar signaling in Arabidopsis. [PMID: 24223111] Data show that TRANSPARENT TESTA GLABRA 2 (TTG2) is a specific activator of TRYPTICHON protein (TRY) by binding to W-boxes in a minimal promoter fragment of TRY. [PMID: 25304203] TTG2 regulates two vacuolar transport genes, TT12 and AHA10. TTG2 is dependent on TTG1 WDR protein. TTG2 and TTG1 physically interact.TTG2 can bind to the upstream regulatory region of TT12 suggesting that TTG2 directly regulates TT12. [PMID: 27046632]

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT2G37260.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Not induced by salicylic acid or wounding. {ECO:0000269\|PubMed:12084832}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source	Upstream Regulator (A: Activate/R: Repress)
ATRM	AT1G56650 (A), AT1G63650 (A), AT1G66390 (A), AT2G37260 (A), AT2G46410 (R), AT3G13540 (A), AT3G27920 (A), AT4G09820 (A), AT4G36920 (A), AT5G14750 (A), AT5G35550 (A), AT5G41315 (A), AT5G53200 (R)

Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source	Target Gene (A: Activate/R: Repress)
ATRM	AT1G79840(A), AT2G37260(A)

Interaction ? help Back to Top
Source	Intact With
BioGRID	AT2G37260

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT2G37260

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AB289607	0.0	AB289607.1 Arabidopsis thaliana TTG2 mRNA for WRKY transcription factor, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_181263.2	0.0	WRKY family transcription factor family protein
Swissprot	Q9ZUU0	0.0	WRK44_ARATH; WRKY transcription factor 44
TrEMBL	A0A384L2V3	0.0	A0A384L2V3_ARATH; WRKY44
TrEMBL	A7BI37	0.0	A7BI37_ARATH; WRKY transcription factor
STRING	AT2G37260.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM8362	27	40
Representative plant	OGRP10259	8	10

Link Out ? help Back to Top
Phytozome	AT2G37260.1
Entrez Gene	818303
iHOP	AT2G37260
wikigenes	AT2G37260

Publications ? help Back to Top
Eulgem T,Rushton PJ,Robatzek S,Somssich IE The WRKY superfamily of plant transcription factors. Trends Plant Sci., 2000. 5(5): p. 199-206 [PMID:10785665] Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Johnson CS,Kolevski B,Smyth DR TRANSPARENT TESTA GLABRA2, a trichome and seed coat development gene of Arabidopsis, encodes a WRKY transcription factor. Plant Cell, 2002. 14(6): p. 1359-75 [PMID:12084832] Marles MA,Ray H,Gruber MY New perspectives on proanthocyanidin biochemistry and molecular regulation. Phytochemistry, 2003. 64(2): p. 367-83 [PMID:12943753] Debeaujon I, et al. Proanthocyanidin-accumulating cells in Arabidopsis testa: regulation of differentiation and role in seed development. Plant Cell, 2003. 15(11): p. 2514-31 [PMID:14555692] Western TL, et al. MUCILAGE-MODIFIED4 encodes a putative pectin biosynthetic enzyme developmentally regulated by APETALA2, TRANSPARENT TESTA GLABRA1, and GLABRA2 in the Arabidopsis seed coat. Plant Physiol., 2004. 134(1): p. 296-306 [PMID:14701918] Garcia D,Fitz Gerald JN,Berger F Maternal control of integument cell elongation and zygotic control of endosperm growth are coordinated to determine seed size in Arabidopsis. Plant Cell, 2005. 17(1): p. 52-60 [PMID:15598800] Ishida T, et al. Arabidopsis TRANSPARENT TESTA GLABRA2 is directly regulated by R2R3 MYB transcription factors and is involved in regulation of GLABRA2 transcription in epidermal differentiation. Plant Cell, 2007. 19(8): p. 2531-43 [PMID:17766401] Zhao M,Morohashi K,Hatlestad G,Grotewold E,Lloyd A The TTG1-bHLH-MYB complex controls trichome cell fate and patterning through direct targeting of regulatory loci. Development, 2008. 135(11): p. 1991-9 [PMID:18434419] Gonzalez A,Mendenhall J,Huo Y,Lloyd A TTG1 complex MYBs, MYB5 and TT2, control outer seed coat differentiation. Dev. Biol., 2009. 325(2): p. 412-21 [PMID:18992236] Dilkes BP, et al. The maternally expressed WRKY transcription factor TTG2 controls lethality in interploidy crosses of Arabidopsis. PLoS Biol., 2008. 6(12): p. 2707-20 [PMID:19071961] Buer CS,Djordjevic MA Architectural phenotypes in the transparent testa mutants of Arabidopsis thaliana. J. Exp. Bot., 2009. 60(3): p. 751-63 [PMID:19129166] Marks MD,Wenger JP,Gilding E,Jilk R,Dixon RA Transcriptome analysis of Arabidopsis wild-type and gl3-sst sim trichomes identifies four additional genes required for trichome development. Mol Plant, 2009. 2(4): p. 803-22 [PMID:19626137] Fang W,Wang Z,Cui R,Li J,Li Y Maternal control of seed size by EOD3/CYP78A6 in Arabidopsis thaliana. Plant J., 2012. 70(6): p. 929-39 [PMID:22251317] Bruex A, et al. A gene regulatory network for root epidermis cell differentiation in Arabidopsis. PLoS Genet., 2012. 8(1): p. e1002446 [PMID:22253603] Kim K,Jiang K,Teng SL,Feldman LJ,Huang H Using biologically interrelated experiments to identify pathway genes in Arabidopsis. Bioinformatics, 2012. 28(6): p. 815-22 [PMID:22271267] Li B, et al. Tobacco TTG2 suppresses resistance to pathogens by sequestering NPR1 from the nucleus. J. Cell. Sci., 2012. 125(Pt 20): p. 4913-22 [PMID:22797922] Xu W, et al. Regulation of flavonoid biosynthesis involves an unexpected complex transcriptional regulation of TT8 expression, in Arabidopsis. New Phytol., 2013. 198(1): p. 59-70 [PMID:23398515] Efroni I, et al. Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses. Dev. Cell, 2013. 24(4): p. 438-45 [PMID:23449474] Simon M, et al. Tissue-specific profiling reveals transcriptome alterations in Arabidopsis mutants lacking morphological phenotypes. Plant Cell, 2013. 25(9): p. 3175-85 [PMID:24014549] Han Y,Zhang X,Wang W,Wang Y,Ming F The suppression of WRKY44 by GIGANTEA-miR172 pathway is involved in drought response of Arabidopsis thaliana. PLoS ONE, 2013. 8(11): p. e73541 [PMID:24223111] Pesch M,Dartan B,Birkenbihl R,Somssich IE,H Arabidopsis TTG2 regulates TRY expression through enhancement of activator complex-triggered activation. Plant Cell, 2014. 26(10): p. 4067-83 [PMID:25304203] Ranocha P,Francoz E,Burlat V,Dunand C Expression of PRX36, PMEI6 and SBT1.7 is controlled by complex transcription factor regulatory networks for proper seed coat mucilage extrusion. Plant Signal Behav, 2014. 9(11): p. e977734 [PMID:25531128] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178] Verweij W, et al. Functionally Similar WRKY Proteins Regulate Vacuolar Acidification in Petunia and Hair Development in Arabidopsis. Plant Cell, 2016. 28(3): p. 786-803 [PMID:26977085] Gonzalez A, et al. TTG2 controls the developmental regulation of seed coat tannins in Arabidopsis by regulating vacuolar transport steps in the proanthocyanidin pathway. Dev. Biol., 2016. 419(1): p. 54-63 [PMID:27046632] Hassani-Pak K, et al. Developing integrated crop knowledge networks to advance candidate gene discovery. Appl Transl Genom, 2016. 11: p. 18-26 [PMID:28018846]