PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT2G30250.1
Common NameATWRKY25, T9D9.6, WRKY25
Organism
Arabidopsis thaliana
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family WRKY
Protein Properties Length: 393aa    MW: 44133.6 Da    PI: 6.4324
Description WRKY DNA-binding protein 25
Gene Model
Gene Model ID Type Source Coding Sequence
AT2G30250.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1WRKY94.76.7e-30166223260
                  --SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS-- CS
         WRKY   2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhek 60 
                  +Dgy WrKYGqK+vk+se+prsY++Ct+++C +kk ve+++ d++++ei+Y+g Hnh+k
  AT2G30250.1 166 NDGYGWRKYGQKQVKKSENPRSYFKCTYPDCVSKKIVETAS-DGQITEIIYKGGHNHPK 223
                  8***************************************9.***************85 PP
2WRKY103.41.2e-32329385359
                  -SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS
         WRKY   3 DgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 
                  Dg++WrKYGqK+vkg+++prsYY+Ct++gC vkk+versa d+++v +tYeg+Hnh+
  AT2G30250.1 329 DGFRWRKYGQKVVKGNTNPRSYYKCTFQGCGVKKQVERSAADERAVLTTYEGRHNHD 385
                  9*******************************************************7 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:2.20.25.801.2E-24158224IPR003657WRKY domain
SuperFamilySSF1182907.45E-23163224IPR003657WRKY domain
SMARTSM007744.9E-30165223IPR003657WRKY domain
PfamPF031063.2E-22166222IPR003657WRKY domain
PROSITE profilePS5081120.346166224IPR003657WRKY domain
Gene3DG3DSA:2.20.25.801.0E-33312387IPR003657WRKY domain
SuperFamilySSF1182906.41E-28319387IPR003657WRKY domain
PROSITE profilePS5081133.545322387IPR003657WRKY domain
SMARTSM007748.3E-38327386IPR003657WRKY domain
PfamPF031064.4E-25329385IPR003657WRKY domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009409Biological Processresponse to cold
GO:0009651Biological Processresponse to salt stress
GO:0070370Biological Processcellular heat acclimation
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
GO:0043565Molecular Functionsequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009001anatomyfruit
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025281anatomypollen
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 393 aa     Download sequence    Send to blast
MSSTSFTDLL GSSGVDCYED DEDLRVSGSS FGGYYPERTG SGLPKFKTAQ PPPLPISQSS  60
HNFTFSDYLD SPLLLSSSHS LISPTTGTFP LQGFNGTTNN HSDFPWQLQS QPSNASSALQ  120
ETYGVQDHEK KQEMIPNEIA TQNNNQSFGT ERQIKIPAYM VSRNSNDGYG WRKYGQKQVK  180
KSENPRSYFK CTYPDCVSKK IVETASDGQI TEIIYKGGHN HPKPEFTKRP SQSSLPSSVN  240
GRRLFNPASV VSEPHDQSEN SSISFDYSDL EQKSFKSEYG EIDEEEEQPE MKRMKREGED  300
EGMSIEVSKG VKEPRVVVQT ISDIDVLIDG FRWRKYGQKV VKGNTNPRSY YKCTFQGCGV  360
KKQVERSAAD ERAVLTTYEG RHNHDIPTAL RRS
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
1wj2_A5e-32318387877Probable WRKY transcription factor 4
2lex_A5e-32318387877Probable WRKY transcription factor 4
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.122350.0leaf| root
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO1453604780.0
Genevisible267246_at0.0
Expression AtlasAT2G30250-
AtGenExpressAT2G30250-
ATTED-IIAT2G30250-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Highly expressed in roots and at lower levels in leaves, stems and seeds. {ECO:0000269|PubMed:18839316}.
Functional Description ? help Back to Top
Source Description
TAIRmember of WRKY Transcription Factor; Group I. Located in nucleus. Involved in response to various abiotic stresses - especially salt stress.
UniProtTranscription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Functions with WRKY33 as positive regulator of salt stress response and abscisic acid (ABA) signaling (PubMed:18839316). Plays a partial role in heat stress tolerance (PubMed:19125253). Functions with WRKY26 and WRKY33 as positive regulator of plant thermotolerance by partially participating in ethylene-response signal transduction pathway (PubMed:21336597). {ECO:0000250|UniProtKB:Q9SI37, ECO:0000269|PubMed:18839316, ECO:0000269|PubMed:19125253, ECO:0000269|PubMed:21336597}.
Function -- GeneRIF ? help Back to Top
  1. WRKY25 functions as a transcription factor with a role in SA-mediated defense responses to Pseudomonas syringae.
    [PMID: 17214894]
  2. overexpression of WRKY25 or WRKY33 was sufficient to increase Arabidopsis NaCl tolerance, while increasing sensitivity to abscisic acid
    [PMID: 18839316]
  3. wrky25 mutants exhibit reduced thermotolerance at different stages of growth.
    [PMID: 19125253]
  4. AtWRKY25 and AtWRKY26 were gradually induced during heat and cold treatments, whereas AtWRKY33 was suppressed by heat treatment and induced rapidly during cold stress.
    [PMID: 20709683]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00282DAP27203113Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT2G30250.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By salt stress (PubMed:18839316). Induced by heat stress (PubMed:19125253). {ECO:0000269|PubMed:18839316, ECO:0000269|PubMed:19125253}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source Upstream Regulator (A: Activate/R: Repress)
ATRM AT5G59820 (A)
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT1G74310(A), AT2G14610(R), AT2G26150(A), AT4G36990(A), AT5G62020(A)
Regulation -- Hormone ? help Back to Top
Source Hormone
AHDabscisic acid
Interaction ? help Back to Top
Source Intact With
IntActSearch O22921
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: No visible phenotype under normal growth conditions. {ECO:0000269|PubMed:18839316}.
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT2G30250
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAF4183090.0AF418309.1 Arabidopsis thaliana WRKY transcription factor 25 (WRKY25) mRNA, complete cds.
GenBankAY0627200.0AY062720.1 Arabidopsis thaliana putative WRKY-type DNA binding protein (At2g30250; T9D9.6) mRNA, complete cds.
GenBankAY1146500.0AY114650.1 Arabidopsis thaliana putative WRKY-type DNA binding protein (At2g30250) mRNA, complete cds.
GenBankAY1363180.0AY136318.1 Arabidopsis thaliana putative WRKY-type DNA binding protein (At2g30250) mRNA, complete cds.
GenBankBT0084820.0BT008482.1 Arabidopsis thaliana At2g30250 gene, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_180584.10.0WRKY DNA-binding protein 25
SwissprotO229210.0WRK25_ARATH; Probable WRKY transcription factor 25
TrEMBLA0A178VTV30.0A0A178VTV3_ARATH; WRKY25
STRINGAT2G30250.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM116031733
Representative plantOGRP1417875
Publications ? help Back to Top
  1. Eulgem T,Rushton PJ,Robatzek S,Somssich IE
    The WRKY superfamily of plant transcription factors.
    Trends Plant Sci., 2000. 5(5): p. 199-206
    [PMID:10785665]
  2. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  3. Folta KM,Pontin MA,Karlin-Neumann G,Bottini R,Spalding EP
    Genomic and physiological studies of early cryptochrome 1 action demonstrate roles for auxin and gibberellin in the control of hypocotyl growth by blue light.
    Plant J., 2003. 36(2): p. 203-14
    [PMID:14535885]
  4. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
    [PMID:14593172]
  5. Rizhsky L,Davletova S,Liang H,Mittler R
    The zinc finger protein Zat12 is required for cytosolic ascorbate peroxidase 1 expression during oxidative stress in Arabidopsis.
    J. Biol. Chem., 2004. 279(12): p. 11736-43
    [PMID:14722088]
  6. Koroleva OA,Tomlinson ML,Leader D,Shaw P,Doonan JH
    High-throughput protein localization in Arabidopsis using Agrobacterium-mediated transient expression of GFP-ORF fusions.
    Plant J., 2005. 41(1): p. 162-74
    [PMID:15610358]
  7. Andreasson E, et al.
    The MAP kinase substrate MKS1 is a regulator of plant defense responses.
    EMBO J., 2005. 24(14): p. 2579-89
    [PMID:15990873]
  8. Duarte JM, et al.
    Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis.
    Mol. Biol. Evol., 2006. 23(2): p. 469-78
    [PMID:16280546]
  9. Zheng Z,Mosher SL,Fan B,Klessig DF,Chen Z
    Functional analysis of Arabidopsis WRKY25 transcription factor in plant defense against Pseudomonas syringae.
    BMC Plant Biol., 2007. 7: p. 2
    [PMID:17214894]
  10. Koornneef A,Pieterse CM
    Cross talk in defense signaling.
    Plant Physiol., 2008. 146(3): p. 839-44
    [PMID:18316638]
  11. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
    [PMID:18650403]
  12. Kim KC,Lai Z,Fan B,Chen Z
    Arabidopsis WRKY38 and WRKY62 transcription factors interact with histone deacetylase 19 in basal defense.
    Plant Cell, 2008. 20(9): p. 2357-71
    [PMID:18776063]
  13. Jiang Y,Deyholos MK
    Functional characterization of Arabidopsis NaCl-inducible WRKY25 and WRKY33 transcription factors in abiotic stresses.
    Plant Mol. Biol., 2009. 69(1-2): p. 91-105
    [PMID:18839316]
  14. Ahlfors R,Brosch
    Nitric oxide modulates ozone-induced cell death, hormone biosynthesis and gene expression in Arabidopsis thaliana.
    Plant J., 2009. 58(1): p. 1-12
    [PMID:19054359]
  15. Li S,Fu Q,Huang W,Yu D
    Functional analysis of an Arabidopsis transcription factor WRKY25 in heat stress.
    Plant Cell Rep., 2009. 28(4): p. 683-93
    [PMID:19125253]
  16. Fu QT,Yu DQ
    [Expression profiles of AtWRKY25, AtWRKY26 and AtWRKY33 under abiotic stresses.].
    Yi Chuan, 2010. 32(8): p. 848-56
    [PMID:20709683]
  17. Shin R,Jez JM,Basra A,Zhang B,Schachtman DP
    14-3-3 proteins fine-tune plant nutrient metabolism.
    FEBS Lett., 2011. 585(1): p. 143-7
    [PMID:21094157]
  18. Petersen K, et al.
    Arabidopsis MKS1 is involved in basal immunity and requires an intact N-terminal domain for proper function.
    PLoS ONE, 2010. 5(12): p. e14364
    [PMID:21203436]
  19. Li S,Fu Q,Chen L,Huang W,Yu D
    Arabidopsis thaliana WRKY25, WRKY26, and WRKY33 coordinate induction of plant thermotolerance.
    Planta, 2011. 233(6): p. 1237-52
    [PMID:21336597]
  20. Lai Z, et al.
    Arabidopsis sigma factor binding proteins are activators of the WRKY33 transcription factor in plant defense.
    Plant Cell, 2011. 23(10): p. 3824-41
    [PMID:21990940]
  21. Cheng Y, et al.
    Structural and functional analysis of VQ motif-containing proteins in Arabidopsis as interacting proteins of WRKY transcription factors.
    Plant Physiol., 2012. 159(2): p. 810-25
    [PMID:22535423]
  22. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
    [PMID:24377444]
  23. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]