PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT2G23320.1

Common Name

AtWRKY15, T20D16.5, WRKY15

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

WRKY

Protein Properties

Length: 317aa MW: 34263.6 Da PI: 10.3009

Description

WRKY DNA-binding protein 15

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT2G23320.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	100	1.5e-31	241	298	3	59
-SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 3 DgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 D y+WrKYGqK++kgs++pr+YY+C+s gCp++k+ver+a+d +++++tYeg+Hnh+ AT2G23320.1 241 DDYSWRKYGQKPIKGSPHPRGYYKCSSVrGCPARKHVERAADDSSMLIVTYEGDHNHS 298 99**********************988**************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF10533	6.4E-16	190	237	IPR018872	Zn-cluster domain
Gene3D	G3DSA:2.20.25.80	4.2E-33	228	298	IPR003657	WRKY domain
PROSITE profile	PS50811	30.607	234	300	IPR003657	WRKY domain
SuperFamily	SSF118290	8.76E-27	237	299	IPR003657	WRKY domain
SMART	SM00774	1.7E-37	239	299	IPR003657	WRKY domain
Pfam	PF03106	3.9E-27	241	297	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0010200	Biological Process	response to chitin
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0005516	Molecular Function	calmodulin binding
GO:0043565	Molecular Function	sequence-specific DNA binding
GO:0044212	Molecular Function	transcription regulatory region DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000084	anatomy	plant sperm cell
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0008019	anatomy	leaf lamina base
PO:0009005	anatomy	root
PO:0009006	anatomy	shoot system
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0025195	anatomy	pollen tube cell
PO:0025281	anatomy	pollen
PO:0001016	developmental stage	L mature pollen stage
PO:0001017	developmental stage	M germinated pollen stage
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007064	developmental stage	LP.12 twelve leaves visible stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 317 aa Download sequence Send to blast
MAVELMTRNY ISGVGADSFA VQEAAASGLK SIENFIGLMS RDSFNSDQPS SSSASASASA 60 AADLESARNT TADAAVSKFK RVISLLDRTR TGHARFRRAP VHVISPVLLQ EEPKTTPFQS 120 PLPPPPQMIR KGSFSSSMKT IDFSSLSSVT TESDNQKKIH HHQRPSETAP FASQTQSLST 180 TVSSFSKSTK RKCNSENLLT GKCASASSSG RCHCSKKRKI KQRRIIRVPA ISAKMSDVPP 240 DDYSWRKYGQ KPIKGSPHPR GYYKCSSVRG CPARKHVERA ADDSSMLIVT YEGDHNHSLS 300 AADLAGAAVA DLILESS

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	2e-22	227	299	2	73	WRKY transcription factor 1
Search in ModeBase

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.24883	0.0	flower\| leaf\| root\| seed\| vegetative tissue

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	30682038	0.0
Genevisible	245051_at	0.0
Expression Atlas	AT2G23320	-
AtGenExpress	AT2G23320	-
ATTED-II	AT2G23320	-

Functional Description ? help Back to Top
Source	Description
TAIR	Encodes WRKY DNA-binding protein 15 (WRKY15).
UniProt	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). {ECO:0000250}.

Function -- GeneRIF ? help Back to Top
WRKY15 transcription factor modulates plant growth and salt/osmotic stress responses. [PMID: 23169634]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00275	DAP	27203113	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT2G23320.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By salicylic acid. {ECO:0000269\|PubMed:11449049}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT2G23320

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AF224704	0.0	AF224704.1 Arabidopsis thaliana WRKY DNA-binding protein 15 (WRKY15) mRNA, complete cds.
GenBank	AF370194	0.0	AF370194.1 Arabidopsis thaliana putative WRKY-type DNA-binding protein (At2g23320) mRNA, complete cds.
GenBank	AY062950	0.0	AY062950.1 Arabidopsis thaliana putative WRKY-type DNA-binding protein (At2g23320) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_179913.1	0.0	WRKY DNA-binding protein 15
Swissprot	O22176	0.0	WRK15_ARATH; Probable WRKY transcription factor 15
TrEMBL	A0A178VVT3	0.0	A0A178VVT3_ARATH; WRKY15
STRING	AT2G23320.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM1715	28	87
Representative plant	OGRP14	17	875

Link Out ? help Back to Top
Phytozome	AT2G23320.1
Entrez Gene	816864
iHOP	AT2G23320
wikigenes	AT2G23320

Publications ? help Back to Top
Eulgem T,Rushton PJ,Robatzek S,Somssich IE The WRKY superfamily of plant transcription factors. Trends Plant Sci., 2000. 5(5): p. 199-206 [PMID:10785665] Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Yu D,Chen C,Chen Z Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression. Plant Cell, 2001. 13(7): p. 1527-40 [PMID:11449049] Mahalingam R, et al. Characterizing the stress/defense transcriptome of Arabidopsis. Genome Biol., 2003. 4(3): p. R20 [PMID:12620105] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Navarro L, et al. The transcriptional innate immune response to flg22. Interplay and overlap with Avr gene-dependent defense responses and bacterial pathogenesis. Plant Physiol., 2004. 135(2): p. 1113-28 [PMID:15181213] Guti Phylogenetic profiling of the Arabidopsis thaliana proteome: what proteins distinguish plants from other organisms? Genome Biol., 2004. 5(8): p. R53 [PMID:15287975] Miao Y,Laun T,Zimmermann P,Zentgraf U Targets of the WRKY53 transcription factor and its role during leaf senescence in Arabidopsis. Plant Mol. Biol., 2004. 55(6): p. 853-67 [PMID:15604721] Park CY, et al. WRKY group IId transcription factors interact with calmodulin. FEBS Lett., 2005. 579(6): p. 1545-50 [PMID:15733871] Devoto A, et al. Expression profiling reveals COI1 to be a key regulator of genes involved in wound- and methyl jasmonate-induced secondary metabolism, defence, and hormone interactions. Plant Mol. Biol., 2005. 58(4): p. 497-513 [PMID:16021335] Phelps-Durr TL,Thomas J,Vahab P,Timmermans MC Maize rough sheath2 and its Arabidopsis orthologue ASYMMETRIC LEAVES1 interact with HIRA, a predicted histone chaperone, to maintain knox gene silencing and determinacy during organogenesis. Plant Cell, 2005. 17(11): p. 2886-98 [PMID:16243907] Tosti N, et al. Gene expression profiles of O3-treated Arabidopsis plants. Plant Cell Environ., 2006. 29(9): p. 1686-702 [PMID:16913859] Cao D,Cheng H,Wu W,Soo HM,Peng J Gibberellin mobilizes distinct DELLA-dependent transcriptomes to regulate seed germination and floral development in Arabidopsis. Plant Physiol., 2006. 142(2): p. 509-25 [PMID:16920880] AbuQamar S, et al. Expression profiling and mutant analysis reveals complex regulatory networks involved in Arabidopsis response to Botrytis infection. Plant J., 2006. 48(1): p. 28-44 [PMID:16925600] Journot-Catalino N,Somssich IE,Roby D,Kroj T The transcription factors WRKY11 and WRKY17 act as negative regulators of basal resistance in Arabidopsis thaliana. Plant Cell, 2006. 18(11): p. 3289-302 [PMID:17114354] Qutob D, et al. Phytotoxicity and innate immune responses induced by Nep1-like proteins. Plant Cell, 2006. 18(12): p. 3721-44 [PMID:17194768] Libault M,Wan J,Czechowski T,Udvardi M,Stacey G Identification of 118 Arabidopsis transcription factor and 30 ubiquitin-ligase genes responding to chitin, a plant-defense elicitor. Mol. Plant Microbe Interact., 2007. 20(8): p. 900-11 [PMID:17722694] Ramel F, et al. Genome-wide interacting effects of sucrose and herbicide-mediated stress in Arabidopsis thaliana: novel insights into atrazine toxicity and sucrose-induced tolerance. BMC Genomics, 2007. 8: p. 450 [PMID:18053238] Ho LH, et al. Identification of regulatory pathways controlling gene expression of stress-responsive mitochondrial proteins in Arabidopsis. Plant Physiol., 2008. 147(4): p. 1858-73 [PMID:18567827] Ascencio-Ib Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection. Plant Physiol., 2008. 148(1): p. 436-54 [PMID:18650403] Wang Y, et al. Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis. Plant Physiol., 2008. 148(3): p. 1201-11 [PMID:18775970] Jakoby MJ, et al. Transcriptional profiling of mature Arabidopsis trichomes reveals that NOECK encodes the MIXTA-like transcriptional regulator MYB106. Plant Physiol., 2008. 148(3): p. 1583-602 [PMID:18805951] Inz A subcellular localization compendium of hydrogen peroxide-induced proteins. Plant Cell Environ., 2012. 35(2): p. 308-20 [PMID:21443605] Vanderauwera S, et al. AtWRKY15 perturbation abolishes the mitochondrial stress response that steers osmotic stress tolerance in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A., 2012. 109(49): p. 20113-8 [PMID:23169634] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Van Aken O, et al. Mitochondrial and Chloroplast Stress Responses Are Modulated in Distinct Touch and Chemical Inhibition Phases. Plant Physiol., 2016. 171(3): p. 2150-65 [PMID:27208304] Liu F, et al. Interactions of WRKY15 and WRKY33 transcription factors and their roles in the resistance of oilseed rape to Sclerotinia infection. Plant Biotechnol. J., 2018. 16(4): p. 911-925 [PMID:28929638]