PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT2G20880.1

Common Name

AtERF53, ERF053, ERF53, F5H14.15

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

ERF

Protein Properties

Length: 336aa MW: 38397.7 Da PI: 6.4126

Description

ERF family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT2G20880.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	AP2	61.6	1.7e-19	186	236	2	56
AP2 2 gykGVrwdkkrgrWvAeIrdpsengkrkrfslgkfgtaeeAakaaiaarkklege 56 y+GVr+++ +g+WvAeIr p++ r+r +lg+f+taeeAa a+++ + kl+ge AT2G20880.1 186 LYRGVRQRH-WGKWVAEIRKPRN---RTRLWLGTFDTAEEAAMAYDREAFKLRGE 236 59****9.******954...5************************97 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
CDD	cd00018	3.26E-34	185	245	No hit	No description
SuperFamily	SSF54171	1.63E-23	186	244	IPR016177	DNA-binding domain
Gene3D	G3DSA:3.30.730.10	1.6E-32	186	244	IPR001471	AP2/ERF domain
PROSITE profile	PS51032	23.235	186	243	IPR001471	AP2/ERF domain
SMART	SM00380	2.8E-41	186	249	IPR001471	AP2/ERF domain
PRINTS	PR00367	2.6E-11	187	198	IPR001471	AP2/ERF domain
Pfam	PF00847	4.3E-14	187	236	IPR001471	AP2/ERF domain
PRINTS	PR00367	2.6E-11	209	225	IPR001471	AP2/ERF domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009651	Biological Process	response to salt stress
GO:0009873	Biological Process	ethylene-activated signaling pathway
GO:2000070	Biological Process	regulation of response to water deprivation
GO:0005634	Cellular Component	nucleus
GO:0005737	Cellular Component	cytoplasm
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000293	anatomy	guard cell
PO:0009029	anatomy	stamen
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 336 aa Download sequence Send to blast
MATAKNKGKS IRVLGTSEAE KKDEMELEEE FQFSSGKYKD SGPGSDMWLG DASSTSPRSL 60 RKTRTFDRHN PYLVSSYATP QPPTTTTCSV SFPFYLPPAI QNQQRFLHPN DPSGQRQQQM 120 ISFDPQQQVQ PYVAQQQQQQ QHLLQYWRDI LKLSPSGRMM MMNMLRQESD LPLTRPPVQP 180 FSATKLYRGV RQRHWGKWVA EIRKPRNRTR LWLGTFDTAE EAAMAYDREA FKLRGETARL 240 NFPELFLNKQ EPTPVHQKQC ETGTTSEDSS RRGEDDSSTA LAVGGVSEET GWAEAWFNAI 300 PEEWGPGSPL WDDYHFPISN HKDDLDATQN SSSDTI

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1gcc_A	3e-20	185	243	1	60	ETHYLENE RESPONSIVE ELEMENT BINDING FACTOR 1
2gcc_A	4e-20	185	243	4	63	ATERF1
3gcc_A	4e-20	185	243	4	63	ATERF1
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Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.27216	0.0	root

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	30681185	0.0
Genevisible	265418_at	0.0
Expression Atlas	AT2G20880	-
AtGenExpress	AT2G20880	-
ATTED-II	AT2G20880	-

Functional Description ? help Back to Top
Source	Description
UniProt	Transcriptional activator involved in abiotic stress tolerance. Can directly regulate stress-related gene expression by binding to the DNA sequence 5'-[AG]CCGAC-3' (DRE element) of their promoter region. Involved in the regulation of stomatal closure movement under drought stress (PubMed:23625358). Acts as a positive regulator of drought stress response (PubMed:22095047). {ECO:0000269\|PubMed:22095047, ECO:0000269\|PubMed:23625358}.

Function -- GeneRIF ? help Back to Top
RGLG2 negatively regulates the drought stress response by mediating AtERF53 transcriptional activity in Arabidopsis.[AtERF53] [PMID: 22095047] Data indicate that expression of AtERF53 in wild-type Arabidopsis was responsive to heat and abscisic acid (ABA) treatment. [PMID: 23625358]

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT2G20880.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced by drought stress (PubMed:18550687, PubMed:22095047, PubMed:23625358). Induced by salt stress (PubMed:22095047). Induced by abscisic acid (ABA) and heat stress (PubMed:23625358). {ECO:0000269\|PubMed:18550687, ECO:0000269\|PubMed:22095047, ECO:0000269\|PubMed:23625358}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT2G20880

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AC006234	0.0	AC006234.4 Arabidopsis thaliana chromosome 2 clone F5H14 map mi148, complete sequence.
GenBank	AY062843	0.0	AY062843.1 Arabidopsis thaliana AP2 domain transcription factor (At2g20880; F5H14.15) mRNA, complete cds.
GenBank	AY081659	0.0	AY081659.1 Arabidopsis thaliana AP2 domain transcription factor (At2g20880) mRNA, complete cds.
GenBank	CP002685	0.0	CP002685.1 Arabidopsis thaliana chromosome 2, complete sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_179685.1	0.0	Integrase-type DNA-binding superfamily protein
Swissprot	Q9SKT1	0.0	ERF53_ARATH; Ethylene-responsive transcription factor ERF053
TrEMBL	A0A178VSD7	0.0	A0A178VSD7_ARATH; ERF53
STRING	AT2G20880.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM2141	27	75
Representative plant	OGRP6	16	1718

Link Out ? help Back to Top
Phytozome	AT2G20880.1
Entrez Gene	816622
iHOP	AT2G20880
wikigenes	AT2G20880

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Koroleva OA,Tomlinson ML,Leader D,Shaw P,Doonan JH High-throughput protein localization in Arabidopsis using Agrobacterium-mediated transient expression of GFP-ORF fusions. Plant J., 2005. 41(1): p. 162-74 [PMID:15610358] Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Nakano T,Suzuki K,Fujimura T,Shinshi H Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol., 2006. 140(2): p. 411-32 [PMID:16407444] Ditt RF, et al. The Arabidopsis thaliana transcriptome in response to Agrobacterium tumefaciens. Mol. Plant Microbe Interact., 2006. 19(6): p. 665-81 [PMID:16776300] Osuna D, et al. Temporal responses of transcripts, enzyme activities and metabolites after adding sucrose to carbon-deprived Arabidopsis seedlings. Plant J., 2007. 49(3): p. 463-91 [PMID:17217462] Ehlting J, et al. Comparative transcriptome analysis of Arabidopsis thaliana infested by diamond back moth (Plutella xylostella) larvae reveals signatures of stress response, secondary metabolism, and signalling. BMC Genomics, 2008. 9: p. 154 [PMID:18400103] Endo A, et al. Drought induction of Arabidopsis 9-cis-epoxycarotenoid dioxygenase occurs in vascular parenchyma cells. Plant Physiol., 2008. 147(4): p. 1984-93 [PMID:18550687] Cheng MC,Hsieh EJ,Chen JH,Chen HY,Lin TP Arabidopsis RGLG2, functioning as a RING E3 ligase, interacts with AtERF53 and negatively regulates the plant drought stress response. Plant Physiol., 2012. 158(1): p. 363-75 [PMID:22095047] Efroni I, et al. Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses. Dev. Cell, 2013. 24(4): p. 438-45 [PMID:23449474] Hsieh EJ,Cheng MC,Lin TP Functional characterization of an abiotic stress-inducible transcription factor AtERF53 in Arabidopsis thaliana. Plant Mol. Biol., 2013. 82(3): p. 223-37 [PMID:23625358] Cheng MC,Hsieh EJ,Chen JH,Chen HY,Lin TP CORRECTION. Arabidopsis RGLG2, Functioning as a RING E3 Ligase, Interacts with AtERF53 and Negatively Regulates the Plant Drought Stress Response. Plant Physiol., 2016. 170(2): p. 1162-3 [PMID:26826724] Huang KC,Lin WC,Cheng WH Salt hypersensitive mutant 9, a nucleolar APUM23 protein, is essential for salt sensitivity in association with the ABA signaling pathway in Arabidopsis. BMC Plant Biol., 2018. 18(1): p. 40 [PMID:29490615]