PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT2G20570.1

Common Name

ATGLK1, F23N11.11, GLK1, GPRI1

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

G2-like

Protein Properties

Length: 420aa MW: 45987.6 Da PI: 6.5412

Description

GBF's pro-rich region-interacting factor 1

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT2G20570.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	G2-like	89.8	2.4e-28	153	207	1	56
G2-like 1 kprlrWtpeLHerFveaveqLGGsekAtPktilelmkvkgLtlehvkSHLQkYRla 56 k +++WtpeLH+rFveaveqL G +kA+P++ilelm+v++Lt+++v+SHLQkYR++ AT2G20570.1 153 KVKVDWTPELHRRFVEAVEQL-GVDKAVPSRILELMGVHCLTRHNVASHLQKYRSH 207 5689***************.******************************86 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS51294	18.721	150	209	IPR017930	Myb domain
Gene3D	G3DSA:1.10.10.60	2.1E-27	151	210	IPR009057	Homeodomain-like
SuperFamily	SSF46689	2.51E-18	152	210	IPR009057	Homeodomain-like
TIGRFAMs	TIGR01557	3.0E-26	153	207	IPR006447	Myb domain, plants
Pfam	PF00249	4.1E-8	156	205	IPR001005	SANT/Myb domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0007165	Biological Process	signal transduction
GO:0009658	Biological Process	chloroplast organization
GO:0009910	Biological Process	negative regulation of flower development
GO:0010380	Biological Process	regulation of chlorophyll biosynthetic process
GO:0010638	Biological Process	positive regulation of organelle organization
GO:0045893	Biological Process	positive regulation of transcription, DNA-templated
GO:1900056	Biological Process	negative regulation of leaf senescence
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0005515	Molecular Function	protein binding
GO:0044212	Molecular Function	transcription regulatory region DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0008019	anatomy	leaf lamina base
PO:0009006	anatomy	shoot system
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007064	developmental stage	LP.12 twelve leaves visible stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 420 aa Download sequence Send to blast
MLALSPATRD GCDGASEFLD TSCGFTIINP EEEEEFPDFA DHGDLLDIID FDDIFGVAGD 60 VLPDLEIDPE ILSGDFSNHM NASSTITTTS DKTDSQGETT KGSSGKGEEV VSKRDDVAAE 120 TVTYDGDSDR KRKYSSSASS KNNRISNNEG KRKVKVDWTP ELHRRFVEAV EQLGVDKAVP 180 SRILELMGVH CLTRHNVASH LQKYRSHRKH LLAREAEAAN WTRKRHIYGV DTGANLNGRT 240 KNGWLAPAPT LGFPPPPPVA VAPPPVHHHH FRPLHVWGHP TVDQSIMPHV WPKHLPPPST 300 AMPNPPFWVS DSPYWHPMHN GTTPYLPTVA TRFRAPPVAG IPHALPPHHT MYKPNLGFGG 360 ARPPVDLHPS KESVDAAIGD VLTRPWLPLP LGLNPPAVDG VMTELHRHGV SEVPPTASCA

Sequence ? help Back to Top

Protein Sequence Length: 420 aa Download sequence Send to blast

MLALSPATRD GCDGASEFLD TSCGFTIINP EEEEEFPDFA DHGDLLDIID FDDIFGVAGD  60
VLPDLEIDPE ILSGDFSNHM NASSTITTTS DKTDSQGETT KGSSGKGEEV VSKRDDVAAE  120
TVTYDGDSDR KRKYSSSASS KNNRISNNEG KRKVKVDWTP ELHRRFVEAV EQLGVDKAVP  180
SRILELMGVH CLTRHNVASH LQKYRSHRKH LLAREAEAAN WTRKRHIYGV DTGANLNGRT  240
KNGWLAPAPT LGFPPPPPVA VAPPPVHHHH FRPLHVWGHP TVDQSIMPHV WPKHLPPPST  300
AMPNPPFWVS DSPYWHPMHN GTTPYLPTVA TRFRAPPVAG IPHALPPHHT MYKPNLGFGG  360
ARPPVDLHPS KESVDAAIGD VLTRPWLPLP LGLNPPAVDG VMTELHRHGV SEVPPTASCA

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1irz_A	4e-15	150	205	2	57	ARR10-B
5lxu_A	4e-15	158	209	6	57	Transcription factor LUX
Search in ModeBase

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.24134	0.0	bud\| seed\| silique\| vegetative tissue

Expression -- Microarray ? help Back to Top
Source	ID	E-value
Genevisible	263715_at	0.0
Expression Atlas	AT2G20570	-
AtGenExpress	AT2G20570	-
ATTED-II	AT2G20570	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed in rosette and cauline leaves. Expressed at low levels in cotyledons and shoots. {ECO:0000269\|PubMed:12220263}.

Functional Description ? help Back to Top
Source	Description
TAIR	Encodes GLK1, Golden2-like 1, one of a pair of partially redundant nuclear transcription factors that regulate chloroplast development in a cell-autonomous manner. GLK2, Golden2-like 2, is encoded by At5g44190. GLK1 and GLK2 regulate the expression of the photosynthetic apparatus.
UniProt	Transcriptional activator that functions with GLK2 to promote chloroplast development. Acts as an activator of nuclear photosynthetic genes involved in chlorophyll biosynthesis, light harvesting, and electron transport. Acts in a cell-autonomous manner to coordinate and maintain the photosynthetic apparatus within individual cells. May function in photosynthetic capacity optimization by integrating responses to variable environmental and endogenous cues (PubMed:11828027, PubMed:12220263, PubMed:17533111, PubMed:18643989, PubMed:19376934, PubMed:19383092, PubMed:19726569). Prevents premature senescence (PubMed:23459204). {ECO:0000269\|PubMed:11828027, ECO:0000269\|PubMed:12220263, ECO:0000269\|PubMed:17533111, ECO:0000269\|PubMed:18643989, ECO:0000269\|PubMed:19376934, ECO:0000269\|PubMed:19383092, ECO:0000269\|PubMed:19726569, ECO:0000269\|PubMed:23459204}.

Function -- GeneRIF ? help Back to Top
GLK1 'regulon' encodes disease defense related proteins and confers resistance to Fusarium graminearum in Arabidopsis [PMID: 17533111] GLK1 and GLK2 are functionally equivalent. They act in a cell-autonomous manner to regulate chloroplast development. When mis-expressed in the phloem, GLKs can be unloaded into adjoining photosynthetic tissue. [PMID: 18643989] GLK1 and GLK2 stimulate expression of chlorophyll biosynthesis and light-harvesting genes. [PMID: 19376934] AtGLK1 may coordinate plastid protein import and nuclear gene expression. [PMID: 19726569] ORE1 interacts with the G2-like transcription factors GLK1 and GLK2, which are important for chloroplast development and maintenance. [PMID: 23459204] Overexpression of GLKs caused up-regulation of not only their direct targets but also non-target nuclear and plastid genes, leading to global induction of chloroplast biogenesis in the root. [PMID: 23749810] data reveal that AtGLK1 is involved in JA-dependent susceptibility to the biotrophic pathogen Hpa Noco2 and in JA-independent resistance to the necrotrophic pathogen B. cinerea [PMID: 24393452] Overexpressors of the Golden2-like transcription factors GLK1 and GLK2 were identified as genomes uncoupled mutants. [PMID: 26876646] plants that overexpress GLK1/2 exhibited an open-stomata phenotype and higher sensitivity to ozone [PMID: 27035938] Plastid signals down-regulate the accumulation of GLK1 through the ubiquitin-proteasome pathway. [PMID: 27821720] P69 binding to GLK proteins may interfere with the interaction between GLK proteins and upstream transcription factors, causing defective binding of GLK proteins to their targets. [PMID: 27964999] silencing of AtRAP by AtlsiRNA-1 upon bacterial infection triggers defense responses through regulation of LSU2 and GLK1. [PMID: 28656601]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00022	PBM	24477691	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT2G20570.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By light. Repressed by BZR2. {ECO:0000269\|PubMed:12220263, ECO:0000269\|PubMed:21214652}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source	Upstream Regulator (A: Activate/R: Repress)
ATRM	AT1G19350 (R)

Interaction ? help Back to Top
Source	Intact With
BioGRID	AT2G46270, AT4G36730, AT5G39610, AT1G28360
IntAct	Search Q9SIV3

Phenotype -- Disruption Phenotype ? help Back to Top
Source	Description
UniProt	DISRUPTION PHENOTYPE: No visible phenotype (PubMed:12220263). Pale-green seedlings in double mutants glk1/glk2 (PubMed:23459204). {ECO:0000269\|PubMed:12220263, ECO:0000269\|PubMed:23459204}.

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT2G20570

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AB062489	0.0	AB062489.1 Arabidopsis thaliana mRNA for GPRI1, complete cds.
GenBank	AY026772	0.0	AY026772.1 Arabidopsis thaliana golden2-like transcription factor (GLK1) mRNA, complete cds.
GenBank	AY054600	0.0	AY054600.1 Arabidopsis thaliana Unknown protein (At2g20570; F23N11.11) mRNA, complete cds.
GenBank	AY081485	0.0	AY081485.1 Arabidopsis thaliana unknown protein (At2g20570) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_565476.1	0.0	GBF's pro-rich region-interacting factor 1
Swissprot	Q9SIV3	0.0	GLK1_ARATH; Transcription activator GLK1
TrEMBL	A0A178VXX4	0.0	A0A178VXX4_ARATH; GPRI1
STRING	AT2G20570.2	0.0	(Arabidopsis thaliana)

Link Out ? help Back to Top
Phytozome	AT2G20570.1
Entrez Gene	816579
iHOP	AT2G20570
wikigenes	AT2G20570

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Tamai H,Iwabuchi M,Meshi T Arabidopsis GARP transcriptional activators interact with the Pro-rich activation domain shared by G-box-binding bZIP factors. Plant Cell Physiol., 2002. 43(1): p. 99-107 [PMID:11828027] Fitter DW,Martin DJ,Copley MJ,Scotland RW,Langdale JA GLK gene pairs regulate chloroplast development in diverse plant species. Plant J., 2002. 31(6): p. 713-27 [PMID:12220263] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Savitch LV,Subramaniam R,Allard GC,Singh J The GLK1 'regulon' encodes disease defense related proteins and confers resistance to Fusarium graminearum in Arabidopsis. Biochem. Biophys. Res. Commun., 2007. 359(2): p. 234-8 [PMID:17533111] Gutiérrez RA, et al. Systems approach identifies an organic nitrogen-responsive gene network that is regulated by the master clock control gene CCA1. Proc. Natl. Acad. Sci. U.S.A., 2008. 105(12): p. 4939-44 [PMID:18344319] Waters MT,Moylan EC,Langdale JA GLK transcription factors regulate chloroplast development in a cell-autonomous manner. Plant J., 2008. 56(3): p. 432-44 [PMID:18643989] Waters MT, et al. GLK transcription factors coordinate expression of the photosynthetic apparatus in Arabidopsis. Plant Cell, 2009. 21(4): p. 1109-28 [PMID:19376934] Bravo-Garcia A,Yasumura Y,Langdale JA Specialization of the Golden2-like regulatory pathway during land plant evolution. New Phytol., 2009. 183(1): p. 133-41 [PMID:19383092] Kakizaki T, et al. Coordination of plastid protein import and nuclear gene expression by plastid-to-nucleus retrograde signaling. Plant Physiol., 2009. 151(3): p. 1339-53 [PMID:19726569] Perochon A, et al. Interaction of a plant pseudo-response regulator with a calmodulin-like protein. Biochem. Biophys. Res. Commun., 2010. 398(4): p. 747-51 [PMID:20627089] Yu X, et al. A brassinosteroid transcriptional network revealed by genome-wide identification of BESI target genes in Arabidopsis thaliana. Plant J., 2011. 65(4): p. 634-46 [PMID:21214652] Arabidopsis Interactome Mapping Consortium Evidence for network evolution in an Arabidopsis interactome map. Science, 2011. 333(6042): p. 601-7 [PMID:21798944] Yu B, et al. Arabidopsis cpSRP54 regulates carotenoid accumulation in Arabidopsis and Brassica napus. J. Exp. Bot., 2012. 63(14): p. 5189-202 [PMID:22791829] Wang P, et al. Evolution of GOLDEN2-LIKE gene function in C(3) and C (4) plants. Planta, 2013. 237(2): p. 481-95 [PMID:22968911] Efroni I, et al. Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses. Dev. Cell, 2013. 24(4): p. 438-45 [PMID:23449474] Rauf M, et al. ORE1 balances leaf senescence against maintenance by antagonizing G2-like-mediated transcription. EMBO Rep., 2013. 14(4): p. 382-8 [PMID:23459204] Kobayashi K, et al. Photosynthesis of root chloroplasts developed in Arabidopsis lines overexpressing GOLDEN2-LIKE transcription factors. Plant Cell Physiol., 2013. 54(8): p. 1365-77 [PMID:23749810] Murmu J, et al. Arabidopsis GOLDEN2-LIKE (GLK) transcription factors activate jasmonic acid (JA)-dependent disease susceptibility to the biotrophic pathogen Hyaloperonospora arabidopsidis, as well as JA-independent plant immunity against the necrotrophic pathogen Botrytis cinerea. Mol. Plant Pathol., 2014. 15(2): p. 174-84 [PMID:24393452] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178] Leister D,Kleine T Definition of a core module for the nuclear retrograde response to altered organellar gene expression identifies GLK overexpressors as gun mutants. Physiol Plant, 2016. 157(3): p. 297-309 [PMID:26876646] Nagatoshi Y, et al. GOLDEN 2-LIKE transcription factors for chloroplast development affect ozone tolerance through the regulation of stomatal movement. Proc. Natl. Acad. Sci. U.S.A., 2016. 113(15): p. 4218-23 [PMID:27035938] Tokumaru M, et al. Ubiquitin-Proteasome Dependent Regulation of the GOLDEN2-LIKE 1 Transcription Factor in Response to Plastid Signals. Plant Physiol., 2017. 173(1): p. 524-535 [PMID:27821720] Ni F, et al. Turnip Yellow Mosaic Virus P69 Interacts with and Suppresses GLK Transcription Factors to Cause Pale-Green Symptoms in Arabidopsis. Mol Plant, 2017. 10(5): p. 764-766 [PMID:27964999] Wang H,Seo JK,Gao S,Cui X,Jin H Silencing of AtRAP, a target gene of a bacteria-induced small RNA, triggers antibacterial defense responses through activation of LSU2 and down-regulation of GLK1. New Phytol., 2017. 215(3): p. 1144-1155 [PMID:28656601]