PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT1G75540.1

Common Name

AtBBX21, BBX21, F10A5.24, LHUS, STH2

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

DBB

Protein Properties

Length: 331aa MW: 36633.6 Da PI: 6.9099

Description

salt tolerance homolog2

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT1G75540.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	zf-B_box	24.6	5.3e-08	3	34	4	35
zf-B_box 4 rkCpeHeekelqlfCedCqqllCedClleeHk 35 +C+ ++++e++ fC+ ++ lC C + H+ AT1G75540.1 3 IRCDVCDKEEASVFCTADEASLCGGCDHQVHH 34 58***************************974 PP
2	zf-B_box	30.8	6e-10	57	94	3	38
zf-B_box 3 erkCpeHeekelqlfCedCqqllCedClleeHkg..Ht 38 + C+ +++k++ lfC++++ lC+dC +++H Ht AT1G75540.1 57 SPLCDICQDKKALLFCQQDRAILCKDCDSSIHAAneHT 94 579*****************************556776 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50119	10.651	1	47	IPR000315	B-box-type zinc finger
Pfam	PF00643	6.5E-6	3	34	IPR000315	B-box-type zinc finger
CDD	cd00021	1.32E-6	3	47	No hit	No description
SMART	SM00336	1.5E-6	4	47	IPR000315	B-box-type zinc finger
SuperFamily	SSF57845	7.78E-5	50	93	No hit	No description
SMART	SM00336	8.4E-15	55	102	IPR000315	B-box-type zinc finger
PROSITE profile	PS50119	10.424	55	102	IPR000315	B-box-type zinc finger
Pfam	PF00643	3.3E-8	57	95	IPR000315	B-box-type zinc finger
CDD	cd00021	3.01E-11	58	102	No hit	No description

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009640	Biological Process	photomorphogenesis
GO:0009641	Biological Process	shade avoidance
GO:0005634	Cellular Component	nucleus
GO:0000989	Molecular Function	transcription factor activity, transcription factor binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0005515	Molecular Function	protein binding
GO:0008270	Molecular Function	zinc ion binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0009005	anatomy	root
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009030	anatomy	carpel
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 331 aa Download sequence Send to blast
MKIRCDVCDK EEASVFCTAD EASLCGGCDH QVHHANKLAS KHLRFSLLYP SSSNTSSPLC 60 DICQDKKALL FCQQDRAILC KDCDSSIHAA NEHTKKHDRF LLTGVKLSAT SSVYKPTSKS 120 SSSSSSNQDF SVPGSSISNP PPLKKPLSAP PQSNKIQPFS KINGGDASVN QWGSTSTISE 180 YLMDTLPGWH VEDFLDSSLP TYGFSKSGDD DGVLPYMEPE DDNNTKRNNN NNNNNNNNTV 240 SLPSKNLGIW VPQIPQTLPS SYPNQYFSQD NNIQFGMYNK ETSPEVVSFA PIQNMKQQGQ 300 NNKRWYDDGG FTVPQITPPP LSSNKKFRSF W

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.34761	0.0	leaf\| root

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	186495629	0.0
Genevisible	262975_at	0.0
Expression Atlas	AT1G75540	-
AtGenExpress	AT1G75540	-
ATTED-II	AT1G75540	-

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription activator that acts as positive regulator of seedling photomorphogenesis (PubMed:17965270). Acts downstream of COP1 and play an important role in early and long-term adjustment of the shade avoidance syndrome (SAS) responses in natural environments (PubMed:21070414). {ECO:0000269\|PubMed:17965270, ECO:0000269\|PubMed:21070414}.

Function -- GeneRIF ? help Back to Top
study identified STH2 (SALT TOLERANCE HOMOLOG2), a B-box protein in Arabidopsis thaliana, as a positive regulator of photomorphogenesis and report that the B-box domain plays a direct role in activating transcription in plants [PMID: 17965270] acts downstream of COP1 in the shade-avoidance syndrome [PMID: 21070414] BBX32 interacts with SALT TOLERANCE HOMOLOG2/BBX21, another B-box protein previously shown to interact with HY5. [PMID: 21632973] our study indicates that BBX21 coordinates with HY5 and ABI5 on the ABI5 promoter and that these transcriptional regulators work in concert to integrate light and ABA signaling in Arabidopsis thaliana. [PMID: 24586210] binds to the T/G-box in the ELONGATED HYPOCOTYL 5 (HY5) promoter and directly activates HY5 expression in the light [PMID: 27325768] AGB1 positively promotes hypocotyl elongation through repressing BBX21 activity. [PMID: 28827171] The second B-box domain in BBX21 is indispensable for its biological function in plants. The structurally intact second B-box domain, but not the first one, is required and necessary for binding of HY5 promoter, activating HY5 expression and promoting photomorphogenesis. [PMID: 29259103] Findings indicate that both B-Box Proteins BBX21 and BBX24 regulate ELONGATED HYPOCOTYL 5 protein (HY5) activity post-transcriptionally, in opposite ways. [PMID: 29439209] BBX21 overexpression improved morphological and physiological attributes, and photosynthetic rates in nonoptimal, high-irradiance conditions, without associated impairment of water use efficiency. [PMID: 29555784] interaction with HRB2 modulates ABI5 locus and stomatal aperture [PMID: 29748960]

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT1G75540.1

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Interaction ? help Back to Top
Source	Intact With
IntAct	Search Q9LQZ7

Phenotype -- Disruption Phenotype ? help Back to Top
Source	Description
UniProt	DISRUPTION PHENOTYPE: Enhanced number of emerged lateral roots. {ECO:0000269\|PubMed:17965270}.

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT1G75540

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AB493536	0.0	AB493536.1 Arabidopsis thaliana At1g75540 mRNA for hypothetical protein, partial cds, clone: RAAt1g75540.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_177686.1	0.0	salt tolerance homolog2
Swissprot	Q9LQZ7	0.0	BBX21_ARATH; B-box zinc finger protein 21
TrEMBL	A0A178WQ55	0.0	A0A178WQ55_ARATH; STH2
STRING	AT1G75540.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM5400	27	48
Representative plant	OGRP2984	11	30

Link Out ? help Back to Top
Phytozome	AT1G75540.1
Entrez Gene	843890
iHOP	AT1G75540
wikigenes	AT1G75540

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Jiao Y, et al. A genome-wide analysis of blue-light regulation of Arabidopsis transcription factor gene expression during seedling development. Plant Physiol., 2003. 133(4): p. 1480-93 [PMID:14605227] Datta S,Hettiarachchi C,Johansson H,Holm M SALT TOLERANCE HOMOLOG2, a B-box protein in Arabidopsis that activates transcription and positively regulates light-mediated development. Plant Cell, 2007. 19(10): p. 3242-55 [PMID:17965270] Ascencio-Ib Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection. Plant Physiol., 2008. 148(1): p. 436-54 [PMID:18650403] Datta S, et al. LZF1/SALT TOLERANCE HOMOLOG3, an Arabidopsis B-box protein involved in light-dependent development and gene expression, undergoes COP1-mediated ubiquitination. Plant Cell, 2008. 20(9): p. 2324-38 [PMID:18796637] Datta S,Johansson H,Hettiarachchi C,Holm M STH2 has 2 B there: An insight into the role of B-box containing proteins in Arabidopsis. Plant Signal Behav, 2008. 3(8): p. 547-8 [PMID:19704462] Khanna R, et al. The Arabidopsis B-box zinc finger family. Plant Cell, 2009. 21(11): p. 3416-20 [PMID:19920209] Crocco CD,Holm M,Yanovsky MJ,Botto JF AtBBX21 and COP1 genetically interact in the regulation of shade avoidance. Plant J., 2010. 64(4): p. 551-62 [PMID:21070414] Crocco CD,Holm M,Yanovsky MJ,Botto JF Function of B-BOX under shade. Plant Signal Behav, 2011. 6(1): p. 101-4 [PMID:21301219] Holtan HE, et al. BBX32, an Arabidopsis B-Box protein, functions in light signaling by suppressing HY5-regulated gene expression and interacting with STH2/BBX21. Plant Physiol., 2011. 156(4): p. 2109-23 [PMID:21632973] Song YH,Lee I,Lee SY,Imaizumi T,Hong JC CONSTANS and ASYMMETRIC LEAVES 1 complex is involved in the induction of FLOWERING LOCUS T in photoperiodic flowering in Arabidopsis. Plant J., 2012. 69(2): p. 332-42 [PMID:21950734] Yamawaki S,Yamashino T,Nakamichi N,Nakanishi H,Mizuno T Light-responsive double B-box containing transcription factors are conserved in Physcomitrella patens. Biosci. Biotechnol. Biochem., 2011. 75(10): p. 2037-41 [PMID:21979077] Sarmiento F The BBX subfamily IV: additional cogs and sprockets to fine-tune light-dependent development. Plant Signal Behav, 2013. 8(4): p. e23831 [PMID:23425851] Xu D, et al. Convergence of Light and ABA signaling on the ABI5 promoter. PLoS Genet., 2014. 10(2): p. e1004197 [PMID:24586210] K MJ,Laxmi A DUF581 is plant specific FCS-like zinc finger involved in protein-protein interaction. PLoS ONE, 2014. 9(6): p. e99074 [PMID:24901469] Xu D, et al. BBX21, an Arabidopsis B-box protein, directly activates HY5 and is targeted by COP1 for 26S proteasome-mediated degradation. Proc. Natl. Acad. Sci. U.S.A., 2016. 113(27): p. 7655-60 [PMID:27325768] Xu DB, et al. The G-Protein β Subunit AGB1 Promotes Hypocotyl Elongation through Inhibiting Transcription Activation Function of BBX21 in Arabidopsis. Mol Plant, 2017. 10(9): p. 1206-1223 [PMID:28827171] Xu D,Jiang Y,Li J,Holm M,Deng XW The B-Box Domain Protein BBX21 Promotes Photomorphogenesis. Plant Physiol., 2018. 176(3): p. 2365-2375 [PMID:29259103] Job N,Yadukrishnan P,Bursch K,Datta S,Johansson H Two B-Box Proteins Regulate Photomorphogenesis by Oppositely Modulating HY5 through their Diverse C-Terminal Domains. Plant Physiol., 2018. 176(4): p. 2963-2976 [PMID:29439209] Crocco CD,Ocampo GG,Ploschuk EL,Mantese A,Botto JF Heterologous Expression of AtBBX21 Enhances the Rate of Photosynthesis and Alleviates Photoinhibition in Solanumtuberosum. Plant Physiol., 2018. 177(1): p. 369-380 [PMID:29555784] Kang X, et al. HRB2 and BBX21 interaction modulates Arabidopsis ABI5 locus and stomatal aperture. Plant Cell Environ., 2018. 41(8): p. 1912-1925 [PMID:29748960]