PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT1G73730.1

Common Name

AtEIL3, ATSLIM, EIL3, F25P22.15, SLIM1

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

EIL

Protein Properties

Length: 567aa MW: 64041.5 Da PI: 5.1139

Description

ETHYLENE-INSENSITIVE3-like 3

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT1G73730.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	EIN3	434.8	8.2e-133	38	401	1	351
XXXXXXXXXXXXXXXXXXXXXXX..XXXXX.XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX CS EIN3 1 eelkkrmwkdqmllkrlkerkkqlledkeaatgakksnksneqarrkkmsraQDgiLkYMlkemevcnaqGfvYgiipekgkpvegasdsLraWWkek 98 ++l++rmwkd+++lkr+ker+k + a +++ +k+++qa+rkkmsraQDgiLkYMlk mevc+++GfvYgiipekgkpv+g+sd++raWWkek AT1G73730.1 38 DDLERRMWKDRVRLKRIKERQKAGS---QGAQTKETPKKISDQAQRKKMSRAQDGILKYMLKLMEVCKVRGFVYGIIPEKGKPVSGSSDNIRAWWKEK 132 79******************854...3445678899******************************************************** PP XXXXXXXXXXXXXXXXXXXXXXXXXXX....XX----STTS-HHHHHHHHHHHSSSSSS-TTS--TTT--HHHH---S--HHHHHHT--TT--.---- CS EIN3 99 vefdrngpaaiskyqaknlilsgesslqtersseshslselqDTtlgSLLsalmqhcdppqrrfplekgvepPWWPtGkelwwgelglskdqgtppyk 196 v+fd+ngpaai+ky+ ++l++++++++ ++++ l++lqD+tlgSLLs+lmqhcdppqr++plekg++pPWWPtG+e+ww +lgl+k+q+ ppy+ AT1G73730.1 133 VKFDKNGPAAIAKYEEECLAFGKSDGN----RNSQFVLQDLQDATLGSLLSSLMQHCDPPQRKYPLEKGTPPPWWPTGNEEWWVKLGLPKSQS-PPYR 225 *****************987777....77999*****************************************************.9* PP -GGG--HHHHHHHHHHHHHHTGGGHHHHHHTTTTSSSSTTT--SHHHHHHHHHHTTTTT-S--XXXX..XX......XXXX....XXXXXXXXXXXXX CS EIN3 197 kphdlkkawkvsvLtavikhmsptieeirelerqskylqdkmsakesfallsvlnqeekecatvsah..ss......slrk....qspkvtlsceqke 282 kphdlkk+wkv+vLtavi+hm p+i++i++++rqsk+lqdkm+akes+++l+vlnqee+++++ s++ +s + +++k ++++++++ AT1G73730.1 226 KPHDLKKMWKVGVLTAVINHMLPDIAKIKRHVRQSKCLQDKMTAKESAIWLAVLNQEESLIQQPSSDngNSnvtethR--RgnnaDRRKPVVNSDSDY 321 *****************************************************************66226887540..3344477888999999 PP XXXXXXXXX.XXXXXXXXXX..................XXXXXXXXXXXXXXXXXXXXX......XXXXXXX.XXXXXXXXXXXXXX CS EIN3 283 dvegkkeskikhvqavktta..................gfpvvrkrkkkpsesakvsskevsrtcqssqfrgsetelifadknsisq 351 dv+g++e + + ++++++++ +++ +r+rk+++ +s +v + q+++ ++ ++++i++d+n+++ AT1G73730.1 322 DVDGTEEASGSVSSKDSRRNqiqkeqptaishsvrdqdKAEKHRRRKRPRIRSGTV-------NRQEEEQPEAQQRNILPDMNHVDA 401 ***88888888888888777899999999977555544444444444444444444.......445566667899999999999875 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF04873	2.1E-126	38	283	No hit	No description
Gene3D	G3DSA:1.10.3180.10	7.6E-71	159	291	IPR023278	Ethylene insensitive 3-like protein, DNA-binding domain
SuperFamily	SSF116768	3.27E-60	164	286	IPR023278	Ethylene insensitive 3-like protein, DNA-binding domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009873	Biological Process	ethylene-activated signaling pathway
GO:0042762	Biological Process	regulation of sulfur metabolic process
GO:0071281	Biological Process	cellular response to iron ion
GO:0005634	Cellular Component	nucleus
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0008019	anatomy	leaf lamina base
PO:0009005	anatomy	root
PO:0009006	anatomy	shoot system
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0025195	anatomy	pollen tube cell
PO:0025281	anatomy	pollen
PO:0001016	developmental stage	L mature pollen stage
PO:0001017	developmental stage	M germinated pollen stage
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007064	developmental stage	LP.12 twelve leaves visible stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 567 aa Download sequence Send to blast
MGDLAMSVAD IRMENEPDDL ASDNVAEIDV SDEEIDADDL ERRMWKDRVR LKRIKERQKA 60 GSQGAQTKET PKKISDQAQR KKMSRAQDGI LKYMLKLMEV CKVRGFVYGI IPEKGKPVSG 120 SSDNIRAWWK EKVKFDKNGP AAIAKYEEEC LAFGKSDGNR NSQFVLQDLQ DATLGSLLSS 180 LMQHCDPPQR KYPLEKGTPP PWWPTGNEEW WVKLGLPKSQ SPPYRKPHDL KKMWKVGVLT 240 AVINHMLPDI AKIKRHVRQS KCLQDKMTAK ESAIWLAVLN QEESLIQQPS SDNGNSNVTE 300 THRRGNNADR RKPVVNSDSD YDVDGTEEAS GSVSSKDSRR NQIQKEQPTA ISHSVRDQDK 360 AEKHRRRKRP RIRSGTVNRQ EEEQPEAQQR NILPDMNHVD APLLEYNING THQEDDVVDP 420 NIALGPEDNG LELVVPEFNN NYTYLPLVNE QTMMPVDERP MLYGPNPNQE LQFGSGYNFY 480 NPSAVFVHNQ EDDILHTQIE MNTQAPPHNS GFEEAPGGVL QPLGLLGNED GVTGSELPQY 540 QSGILSPLTD LDFDYGGFGD DFSWFGA

Sequence ? help Back to Top

Protein Sequence Length: 567 aa Download sequence Send to blast

MGDLAMSVAD IRMENEPDDL ASDNVAEIDV SDEEIDADDL ERRMWKDRVR LKRIKERQKA  60
GSQGAQTKET PKKISDQAQR KKMSRAQDGI LKYMLKLMEV CKVRGFVYGI IPEKGKPVSG  120
SSDNIRAWWK EKVKFDKNGP AAIAKYEEEC LAFGKSDGNR NSQFVLQDLQ DATLGSLLSS  180
LMQHCDPPQR KYPLEKGTPP PWWPTGNEEW WVKLGLPKSQ SPPYRKPHDL KKMWKVGVLT  240
AVINHMLPDI AKIKRHVRQS KCLQDKMTAK ESAIWLAVLN QEESLIQQPS SDNGNSNVTE  300
THRRGNNADR RKPVVNSDSD YDVDGTEEAS GSVSSKDSRR NQIQKEQPTA ISHSVRDQDK  360
AEKHRRRKRP RIRSGTVNRQ EEEQPEAQQR NILPDMNHVD APLLEYNING THQEDDVVDP  420
NIALGPEDNG LELVVPEFNN NYTYLPLVNE QTMMPVDERP MLYGPNPNQE LQFGSGYNFY  480
NPSAVFVHNQ EDDILHTQIE MNTQAPPHNS GFEEAPGGVL QPLGLLGNED GVTGSELPQY  540
QSGILSPLTD LDFDYGGFGD DFSWFGA

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wij_A	3e-87	163	294	9	140	ETHYLENE-INSENSITIVE3-like 3 protein
Search in ModeBase

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	145337501	0.0
Genevisible	260064_at	0.0
Expression Atlas	AT1G73730	-
AtGenExpress	AT1G73730	-
ATTED-II	AT1G73730	-

Functional Description ? help Back to Top
Source	Description
TAIR	Encodes a putative transcription factor involved in ethylene signalling. Isolated DNA binding domain has been shown to bind DNA in vitro.
UniProt	Probable transcription factor that may be involved in the ethylene response pathway. {ECO:0000269\|PubMed:9215635, ECO:0000269\|PubMed:9851977}.

Function -- GeneRIF ? help Back to Top
DNA-binding domain NMR structure of EIL3 [PMID: 15811366] SLIM1 functioned as a central transcriptional regulator, which controlled both the activation of sulfate acquisition and degradation of glucosinolates under -S conditions.[SLIM1] [PMID: 17114350] SLIM1, both through the action of miR395 and independently, is responsible for the increased translocation of sulfate from roots to shoots during sulfate starvation, and also for the increased rate of sulfate reduction. [PMID: 21401744] possible regulatory mechanism of ethylene in sulfur metabolism through direct EIN3-SLIM1 interaction [PMID: 27968996]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00233	DAP	27203113	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT1G73730.1

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source	Target Gene (A: Activate/R: Repress)
ATRM	AT1G16400(R), AT1G16410(R), AT1G78000(A), AT2G22330(R), AT3G19710(A), AT3G56040(R), AT4G31500(R), AT4G39950(A), AT5G43780(A), AT5G60890(R)

Regulation -- Hormone ? help Back to Top
Source	Hormone
AHD	ethylene

Interaction ? help Back to Top
Source	Intact With
IntAct	Search O23116

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT1G73730

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AF004215	0.0	AF004215.1 Arabidopsis thaliana ethylene-insensitive3-like3 (EIL3) mRNA, complete cds.
GenBank	AY070044	0.0	AY070044.1 Arabidopsis thaliana putative ethylene-insensitive protein EIL3 (At1g73730) mRNA, complete cds.
GenBank	AY133839	0.0	AY133839.1 Arabidopsis thaliana clone U18784 putative ethylene-insensitive protein EIL3 (At1g73730) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_177514.1	0.0	ETHYLENE-INSENSITIVE3-like 3
Swissprot	O23116	0.0	EIL3_ARATH; ETHYLENE INSENSITIVE 3-like 3 protein
TrEMBL	A0A178VZZ3	0.0	A0A178VZZ3_ARATH; SLIM1
STRING	AT1G73730.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM4658	25	48
Representative plant	OGRP563	16	82

Link Out ? help Back to Top
Phytozome	AT1G73730.1
Entrez Gene	843708
iHOP	AT1G73730
wikigenes	AT1G73730

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Yamasaki K, et al. Solution structure of the major DNA-binding domain of Arabidopsis thaliana ethylene-insensitive3-like3. J. Mol. Biol., 2005. 348(2): p. 253-64 [PMID:15811366] Wang J, et al. Genomewide nonadditive gene regulation in Arabidopsis allotetraploids. Genetics, 2006. 172(1): p. 507-17 [PMID:16172500] Maruyama-Nakashita A,Nakamura Y,Tohge T,Saito K,Takahashi H Arabidopsis SLIM1 is a central transcriptional regulator of plant sulfur response and metabolism. Plant Cell, 2006. 18(11): p. 3235-51 [PMID:17114350] Binder BM, et al. The Arabidopsis EIN3 binding F-Box proteins EBF1 and EBF2 have distinct but overlapping roles in ethylene signaling. Plant Cell, 2007. 19(2): p. 509-23 [PMID:17307926] Gigolashvili T,Engqvist M,Yatusevich R,Müller C,Flügge UI HAG2/MYB76 and HAG3/MYB29 exert a specific and coordinated control on the regulation of aliphatic glucosinolate biosynthesis in Arabidopsis thaliana. New Phytol., 2008. 177(3): p. 627-42 [PMID:18042203] Van der Ent S, et al. MYB72 is required in early signaling steps of rhizobacteria-induced systemic resistance in Arabidopsis. Plant Physiol., 2008. 146(3): p. 1293-304 [PMID:18218967] Ascencio-Ib Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection. Plant Physiol., 2008. 148(1): p. 436-54 [PMID:18650403] Wang Y, et al. Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis. Plant Physiol., 2008. 148(3): p. 1201-11 [PMID:18775970] Kawashima CG, et al. Sulphur starvation induces the expression of microRNA-395 and one of its target genes but in different cell types. Plant J., 2009. 57(2): p. 313-21 [PMID:18801012] Okazaki Y, et al. A chloroplastic UDP-glucose pyrophosphorylase from Arabidopsis is the committed enzyme for the first step of sulfolipid biosynthesis. Plant Cell, 2009. 21(3): p. 892-909 [PMID:19286968] Wawrzyńska A,Lewandowska M,Sirko A Nicotiana tabacum EIL2 directly regulates expression of at least one tobacco gene induced by sulphur starvation. J. Exp. Bot., 2010. 61(3): p. 889-900 [PMID:20018902] Lewandowska M, et al. A contribution to identification of novel regulators of plant response to sulfur deficiency: characteristics of a tobacco gene UP9C, its protein product and the effects of UP9C silencing. Mol Plant, 2010. 3(2): p. 347-60 [PMID:20147370] Garc Ethylene and nitric oxide involvement in the up-regulation of key genes related to iron acquisition and homeostasis in Arabidopsis. J. Exp. Bot., 2010. 61(14): p. 3885-99 [PMID:20627899] Kawashima CG, et al. Interplay of SLIM1 and miR395 in the regulation of sulfate assimilation in Arabidopsis. Plant J., 2011. 66(5): p. 863-76 [PMID:21401744] Chan PK,Biswas B,Gresshoff PM Classical ethylene insensitive mutants of the Arabidopsis EIN2 orthologue lack the expected 'hypernodulation' response in Lotus japonicus. J Integr Plant Biol, 2013. 55(4): p. 395-408 [PMID:23452324] Luo J, et al. A DELLA gene, RhGAI1, is a direct target of EIN3 and mediates ethylene-regulated rose petal cell expansion via repressing the expression of RhCesA2. J. Exp. Bot., 2013. 64(16): p. 5075-84 [PMID:24014864] Frerigmann H,Gigolashvili T Update on the role of R2R3-MYBs in the regulation of glucosinolates upon sulfur deficiency. Front Plant Sci, 2014. 5: p. 626 [PMID:25426131] Zheng ZL,Zhang B,Leustek T Transceptors at the boundary of nutrient transporters and receptors: a new role for Arabidopsis SULTR1;2 in sulfur sensing. Front Plant Sci, 2014. 5: p. 710 [PMID:25566284] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178] Panda SK,Sunkar R Nutrient- and other stress-responsive microRNAs in plants: Role for thiol-based redox signaling. Plant Signal Behav, 2015. 10(4): p. e1010916 [PMID:25912823] Wawrzyńska A,Sirko A EIN3 interferes with the sulfur deficiency signaling in Arabidopsis thaliana through direct interaction with the SLIM1 transcription factor. Plant Sci., 2016. 253: p. 50-57 [PMID:27968996] Chao Q, et al. Activation of the ethylene gas response pathway in Arabidopsis by the nuclear protein ETHYLENE-INSENSITIVE3 and related proteins. Cell, 1997. 89(7): p. 1133-44 [PMID:9215635] Solano R,Stepanova A,Chao Q,Ecker JR Nuclear events in ethylene signaling: a transcriptional cascade mediated by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1. Genes Dev., 1998. 12(23): p. 3703-14 [PMID:9851977]