PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT1G66470.1

Common Name

AtRHD6, BHLH83, EN112, F28G11.9, RHD6

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

bHLH

Protein Properties

Length: 298aa MW: 32088.1 Da PI: 6.7464

Description

ROOT HAIR DEFECTIVE6

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT1G66470.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	30.6	6e-10	210	251	9	55
HHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 9 ErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 ++RR+ri +++ L+el+P+ +K++ +++LekA+ Y+k Lq AT1G66470.1 210 AKNRRERISERLKILQELVPNG-----TKVDLVTMLEKAISYVKFLQ 251 69****************97.....8****************9 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:4.10.280.10	5.7E-12	201	258	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profile	PS50888	15.32	201	250	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamily	SSF47459	1.44E-13	202	261	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	2.16E-12	203	255	No hit	No description
SMART	SM00353	5.0E-12	207	256	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	4.5E-7	210	251	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0045944	Biological Process	positive regulation of transcription from RNA polymerase II promoter
GO:0048766	Biological Process	root hair initiation
GO:0005634	Cellular Component	nucleus
GO:0005739	Cellular Component	mitochondrion
GO:0001046	Molecular Function	core promoter sequence-specific DNA binding
GO:0001228	Molecular Function	transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
GO:0046983	Molecular Function	protein dimerization activity

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000256	anatomy	root hair cell
PO:0000262	anatomy	trichoblast
PO:0009005	anatomy	root

Sequence ? help Back to Top
Protein Sequence Length: 298 aa Download sequence Send to blast
MALVNDHPNE TNYLSKQNSS SSEDLSSPGL DQPDAAYAGG GGGGGSASSS STMNSDHQQH 60 QGFVFYPSGE DHHNSLMDFN GSSFLNFDHH ESFPPPAISC GGSSGGGGFS FLEGNNMSYG 120 FTNWNHQHHM DIISPRSTET PQGQKDWLYS DSTVVTTGSR NESLSPKSAG NKRSHTGEST 180 QPSKKLSSGV TGKTKPKPTT SPKDPQSLAA KNRRERISER LKILQELVPN GTKVDLVTML 240 EKAISYVKFL QVQVKVLATD EFWPAQGGKA PDISQVKDAI DAILSSSQRD RNSNLITN

Expression -- Microarray ? help Back to Top
Source	ID	E-value
Genevisible	256358_at	0.0
Expression Atlas	AT1G66470	-
AtGenExpress	AT1G66470	-
ATTED-II	AT1G66470	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed constitutively in flowers. {ECO:0000269\|PubMed:12679534}.

Function -- GeneRIF ? help Back to Top
data indicate that AtRHD6 (At1g66470) and AtRSL1 (At5g37800) are bHLH transcription factors that are specifically required for the development of root hairs and act downstream of the genes that regulate epidermal pattern formation [PMID: 17556585] Expression of BdRSL class I genes in root hairless Arabidopsis thaliana root hair defective 6 (Atrhd6) Atrsl1 double mutants, devoid of RSL class I function, restores root hair development indicating that the function of these proteins has been conserved. However, neither AtRSL nor BdRSL class I genes is sufficient for root hair development in A. thaliana [PMID: 27494519] S-nitrosoglutathione and auxin restored the root hair phenotype of the hairless root hair defective 6 (rhd6) mutant. [PMID: 27880005] ET-activated transcription factor ETHYLENE-INSENSITIVE 3 (EIN3) physically interacts with ROOT HAIR DEFECTIVE 6 (RHD6), a well-documented positive regulator of hair cells, and that the two factors directly coactivate the hair length-determining gene RHD6-LIKE 4 (RSL4) to promote root hair elongation. [PMID: 29233944]

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT1G66470.1

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Interaction ? help Back to Top
Source	Intact With
IntAct	Search Q9C707

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT1G66470

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AY128310	0.0	AY128310.1 Arabidopsis thaliana unknown protein (At1g66470) mRNA, complete cds.
GenBank	BT006320	0.0	BT006320.1 Arabidopsis thaliana At1g66470 mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_176820.1	0.0	ROOT HAIR DEFECTIVE6
Swissprot	Q9C707	0.0	BH083_ARATH; Transcription factor bHLH83
TrEMBL	A0A178W430	0.0	A0A178W430_ARATH; RHD6
STRING	AT1G66470.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM4260	28	54
Representative plant	OGRP3532	13	26

Link Out ? help Back to Top
Phytozome	AT1G66470.1
Entrez Gene	842965
iHOP	AT1G66470
wikigenes	AT1G66470

Publications ? help Back to Top
Bernhardt C,Tierney ML Expression of AtPRP3, a proline-rich structural cell wall protein from Arabidopsis, is regulated by cell-type-specific developmental pathways involved in root hair formation. Plant Physiol., 2000. 122(3): p. 705-14 [PMID:10712533] Mendoza L,Alvarez-Buylla ER Genetic regulation of root hair development in Arabidopsis thaliana: a network model. J. Theor. Biol., 2000. 204(3): p. 311-26 [PMID:10816357] Bates TR,Lynch JP Plant growth and phosphorus accumulation of wild type and two root hair mutants of Arabidopsis thaliana (Brassicaceae). Am. J. Bot., 2000. 87(7): p. 958-63 [PMID:10898772] Bates TR,Lynch JP The efficiency of Arabidopsis thaliana (Brassicaceae) root hairs in phosphorus acquisition. Am. J. Bot., 2000. 87(7): p. 964-70 [PMID:10898773] Vissenberg K,Fry SC,Verbelen JP Root hair initiation is coupled to a highly localized increase of xyloglucan endotransglycosylase action in Arabidopsis roots. Plant Physiol., 2001. 127(3): p. 1125-35 [PMID:11706192] Masucci JD,Schiefelbein JW The rhd6 Mutation of Arabidopsis thaliana Alters Root-Hair Initiation through an Auxin- and Ethylene-Associated Process. Plant Physiol., 1994. 106(4): p. 1335-1346 [PMID:12232412] Heim MA, et al. The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity. Mol. Biol. Evol., 2003. 20(5): p. 735-47 [PMID:12679534] Toledo-Ortiz G,Huq E,Quail PH The Arabidopsis basic/helix-loop-helix transcription factor family. Plant Cell, 2003. 15(8): p. 1749-70 [PMID:12897250] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Bailey PC, et al. Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana. Plant Cell, 2003. 15(11): p. 2497-502 [PMID:14600211] Heazlewood JL, et al. Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins. Plant Cell, 2004. 16(1): p. 241-56 [PMID:14671022] Ahn SJ,Shin R,Schachtman DP Expression of KT/KUP genes in Arabidopsis and the role of root hairs in K+ uptake. Plant Physiol., 2004. 134(3): p. 1135-45 [PMID:14988478] Czechowski T,Bari RP,Stitt M,Scheible WR,Udvardi MK Real-time RT-PCR profiling of over 1400 Arabidopsis transcription factors: unprecedented sensitivity reveals novel root- and shoot-specific genes. Plant J., 2004. 38(2): p. 366-79 [PMID:15078338] Yuen CY,Sedbrook JC,Perrin RM,Carroll KL,Masson PH Loss-of-function mutations of ROOT HAIR DEFECTIVE3 suppress root waving, skewing, and epidermal cell file rotation in Arabidopsis. Plant Physiol., 2005. 138(2): p. 701-14 [PMID:15908600] L Bacillus megaterium rhizobacteria promote growth and alter root-system architecture through an auxin- and ethylene-independent signaling mechanism in Arabidopsis thaliana. Mol. Plant Microbe Interact., 2007. 20(2): p. 207-17 [PMID:17313171] Menand B, et al. An ancient mechanism controls the development of cells with a rooting function in land plants. Science, 2007. 316(5830): p. 1477-80 [PMID:17556585] Ohashi-Ito K,Bergmann DC Regulation of the Arabidopsis root vascular initial population by LONESOME HIGHWAY. Development, 2007. 134(16): p. 2959-68 [PMID:17626058] Singh SK,Fischer U,Singh M,Grebe M,Marchant A Insight into the early steps of root hair formation revealed by the procuste1 cellulose synthase mutant of Arabidopsis thaliana. BMC Plant Biol., 2008. 8: p. 57 [PMID:18485206] Contreras-Cornejo HA,Mac Trichoderma virens, a plant beneficial fungus, enhances biomass production and promotes lateral root growth through an auxin-dependent mechanism in Arabidopsis. Plant Physiol., 2009. 149(3): p. 1579-92 [PMID:19176721] Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Lee YC, et al. Growth promotion of Chinese cabbage and Arabidopsis by Piriformospora indica is not stimulated by mycelium-synthesized auxin. Mol. Plant Microbe Interact., 2011. 24(4): p. 421-31 [PMID:21375386] Jang G,Yi K,Pires ND,Menand B,Dolan L RSL genes are sufficient for rhizoid system development in early diverging land plants. Development, 2011. 138(11): p. 2273-81 [PMID:21558375] Arabidopsis Interactome Mapping Consortium Evidence for network evolution in an Arabidopsis interactome map. Science, 2011. 333(6042): p. 601-7 [PMID:21798944] Bruex A, et al. A gene regulatory network for root epidermis cell differentiation in Arabidopsis. PLoS Genet., 2012. 8(1): p. e1002446 [PMID:22253603] Coego A, et al. The TRANSPLANTA collection of Arabidopsis lines: a resource for functional analysis of transcription factors based on their conditional overexpression. Plant J., 2014. 77(6): p. 944-53 [PMID:24456507] Lin CY, et al. Pathways involved in vanadate-induced root hair formation in Arabidopsis. Physiol Plant, 2015. 153(1): p. 137-48 [PMID:24833217] Grierson C,Nielsen E,Ketelaarc T,Schiefelbein J Root hairs. Arabidopsis Book, 2014. 12: p. e0172 [PMID:24982600] Kim CM,Dolan L ROOT HAIR DEFECTIVE SIX-LIKE Class I Genes Promote Root Hair Development in the Grass Brachypodium distachyon. PLoS Genet., 2016. 12(8): p. e1006211 [PMID:27494519] Moro CF, et al. S-nitrosoglutathione promotes cell wall remodelling, alters the transcriptional profile and induces root hair formation in the hairless root hair defective 6 (rhd6) mutant of Arabidopsis thaliana. New Phytol., 2017. 213(4): p. 1771-1786 [PMID:27880005] Bahmani R,Kim DG,Kim JA,Hwang S The Density and Length of Root Hairs Are Enhanced in Response to Cadmium and Arsenic by Modulating Gene Expressions Involved in Fate Determination and Morphogenesis of Root Hairs in Arabidopsis. Front Plant Sci, 2016. 7: p. 1763 [PMID:27933081] Liao X,Lovett B,Fang W,St Leger RJ Metarhizium robertsii produces indole-3-acetic acid, which promotes root growth in Arabidopsis and enhances virulence to insects. Microbiology (Reading, Engl.), 2017. 163(7): p. 980-991 [PMID:28708056] Feng Y, et al. Ethylene promotes root hair growth through coordinated EIN3/EIL1 and RHD6/RSL1 activity in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A., 2017. 114(52): p. 13834-13839 [PMID:29233944] Masucci JD,Schiefelbein JW Hormones act downstream of TTG and GL2 to promote root hair outgrowth during epidermis development in the Arabidopsis root. Plant Cell, 1996. 8(9): p. 1505-17 [PMID:8837505]