PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT1G63030.1

Common Name

ddf2, DREB1E, ERF032, F16M19.20, F16P17.20

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

ERF

Protein Properties

Length: 181aa MW: 20649.4 Da PI: 5.3795

Description

ERF family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT1G63030.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	AP2	47.9	3.4e-15	29	78	2	55
AP2 2 gykGVrwdkkrgrWvAeIrdpsengkr..krfslgkfgtaeeAakaaiaarkkleg 55 y+GVr++ +Wv+e+r+p +r++lg++ ta++Aa+a++ a ++l+g AT1G63030.1 29 IYRGVRRRD-GDKWVCEVREP-----IhqRRVWLGTYPTADMAARAHDVAVLALRG 78 69**988.8**9997.....337***********************98 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
CDD	cd00018	1.74E-25	28	88	No hit	No description
Pfam	PF00847	5.1E-11	28	78	IPR001471	AP2/ERF domain
Gene3D	G3DSA:3.30.730.10	3.5E-28	29	88	IPR001471	AP2/ERF domain
PROSITE profile	PS51032	21.891	29	86	IPR001471	AP2/ERF domain
SMART	SM00380	4.5E-24	29	92	IPR001471	AP2/ERF domain
SuperFamily	SSF54171	1.11E-19	29	88	IPR016177	DNA-binding domain
PRINTS	PR00367	2.2E-7	30	41	IPR001471	AP2/ERF domain
PRINTS	PR00367	2.2E-7	52	68	IPR001471	AP2/ERF domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0010371	Biological Process	regulation of gibberellin biosynthetic process
GO:0016049	Biological Process	cell growth
GO:0048510	Biological Process	regulation of timing of transition from vegetative to reproductive phase
GO:0005634	Cellular Component	nucleus
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000014	anatomy	rosette leaf
PO:0009005	anatomy	root
PO:0009047	anatomy	stem

Sequence ? help Back to Top
Protein Sequence Length: 181 aa Download sequence Send to blast
MENDDITVAE MKPKKRAGRR IFKETRHPIY RGVRRRDGDK WVCEVREPIH QRRVWLGTYP 60 TADMAARAHD VAVLALRGRS ACLNFSDSAW RLPVPASTDP DTIRRTAAEA AEMFRPPEFS 120 TGITVLPSAS EFDTSDEGVA GMMMRLAEEP LMSPPRSYID MNTSVYVDEE MCYEDLSLWS 180 Y

Expression -- Microarray ? help Back to Top
Source	ID	E-value
Genevisible	261101_at	0.0
Expression Atlas	AT1G63030	-
AtGenExpress	AT1G63030	-
ATTED-II	AT1G63030	-

Functional Description ? help Back to Top
Source	Description
TAIR	encodes a member of the DREB subfamily A-1 of ERF/AP2 transcription factor family (DDF2). The protein contains one AP2 domain. There are six members in this subfamily, including CBF1, CBF2, and CBF3. Overexpression of this gene results in the reduction of gibberellic acid biosynthesis. This gene is expressed in all tissues examined, but most abundantly expressed in rosette leaves and stems. Overexpression of DDF1, a putative paralog of this gene, also reduces gibberellic acid biosynthesis and makes the plants more tolerant to high-salinity levels.
UniProt	Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]CCGAC-3'. Binding to the C-repeat/DRE element mediates cold or dehydration-inducible transcription. CBF/DREB1 factors play a key role in freezing tolerance and cold acclimation. {ECO:0000269\|PubMed:11798174}.

Function -- GeneRIF ? help Back to Top
Data indicate that the transcript levels of HAK5 and four transcriptin factor genes DDF2, JLO, bHLH121 and TFII_A were highly up-regulated in response to both -K and salt stress. [PMID: 23825216] Data indicate that both transcription factors DWARF AND DELAYED FLOWERING1 (DDF1) and DDF2 are under selective constraint. [PMID: 26103993]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00104	PBM	26531826	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT1G63030.1

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- Hormone ? help Back to Top
Source	Hormone
AHD	gibberellin

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT1G63030

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AC010795	0.0	AC010795.5 Arabidopsis thaliana chromosome 1 BAC F16M19 genomic sequence, complete sequence.
GenBank	AY560841	0.0	AY560841.1 Arabidopsis thaliana putative AP2/EREBP transcription factor (At1g63030) mRNA, complete cds.
GenBank	BT021917	0.0	BT021917.1 Arabidopsis thaliana At1g63030 mRNA, complete cds.
GenBank	BT025742	0.0	BT025742.1 Arabidopsis thaliana At1g63030 mRNA, complete cds.
GenBank	CP002684	0.0	CP002684.1 Arabidopsis thaliana chromosome 1 sequence.
GenBank	F16P17	0.0	AC011000.3 Sequence of BAC F16P17 from Arabidopsis thaliana chromosome 1, complete sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_001077764.1	1e-132	Integrase-type DNA-binding superfamily protein
Refseq	NP_176491.1	1e-132	Integrase-type DNA-binding superfamily protein
Swissprot	Q9SGJ6	1e-133	DRE1E_ARATH; Dehydration-responsive element-binding protein 1E
TrEMBL	D7KU34	1e-125	D7KU34_ARALL; Uncharacterized protein
STRING	AT1G63030.2	1e-131	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM355	28	187
Representative plant	OGRP6	16	1718

Link Out ? help Back to Top
Phytozome	AT1G63030.1
Entrez Gene	842606
iHOP	AT1G63030
wikigenes	AT1G63030

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Sakuma Y, et al. DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression. Biochem. Biophys. Res. Commun., 2002. 290(3): p. 998-1009 [PMID:11798174] Haake V, et al. Transcription factor CBF4 is a regulator of drought adaptation in Arabidopsis. Plant Physiol., 2002. 130(2): p. 639-48 [PMID:12376631] Brown RL,Kazan K,McGrath KC,Maclean DJ,Manners JM A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis. Plant Physiol., 2003. 132(2): p. 1020-32 [PMID:12805630] Magome H,Yamaguchi S,Hanada A,Kamiya Y,Oda K dwarf and delayed-flowering 1, a novel Arabidopsis mutant deficient in gibberellin biosynthesis because of overexpression of a putative AP2 transcription factor. Plant J., 2004. 37(5): p. 720-9 [PMID:14871311] Ko JH,Han KH,Park S,Yang J Plant body weight-induced secondary growth in Arabidopsis and its transcription phenotype revealed by whole-transcriptome profiling. Plant Physiol., 2004. 135(2): p. 1069-83 [PMID:15194820] Wang Z,Triezenberg SJ,Thomashow MF,Stockinger EJ Multiple hydrophobic motifs in Arabidopsis CBF1 COOH-terminus provide functional redundancy in trans-activation. Plant Mol. Biol., 2005. 58(4): p. 543-59 [PMID:16021338] Nakano T,Suzuki K,Fujimura T,Shinshi H Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol., 2006. 140(2): p. 411-32 [PMID:16407444] Xiong Y,Fei SZ Functional and phylogenetic analysis of a DREB/CBF-like gene in perennial ryegrass (Lolium perenne L.). Planta, 2006. 224(4): p. 878-88 [PMID:16614820] Osuna D, et al. Temporal responses of transcripts, enzyme activities and metabolites after adding sucrose to carbon-deprived Arabidopsis seedlings. Plant J., 2007. 49(3): p. 463-91 [PMID:17217462] Geddy R,Brown GG Genes encoding pentatricopeptide repeat (PPR) proteins are not conserved in location in plant genomes and may be subject to diversifying selection. BMC Genomics, 2007. 8: p. 130 [PMID:17521445] Welling A,Palva ET Involvement of CBF transcription factors in winter hardiness in birch. Plant Physiol., 2008. 147(3): p. 1199-211 [PMID:18467468] Hong JP, et al. Identification and characterization of transcription factors regulating Arabidopsis HAK5. Plant Cell Physiol., 2013. 54(9): p. 1478-90 [PMID:23825216] Lehti-Shiu MD, et al. Molecular Evidence for Functional Divergence and Decay of a Transcription Factor Derived from Whole-Genome Duplication in Arabidopsis thaliana. Plant Physiol., 2015. 168(4): p. 1717-34 [PMID:26103993]