PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT1G51140.1

Common Name

AKS1, BHLH122, EN70, F23H24.3, FBH3

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

bHLH

Protein Properties

Length: 379aa MW: 41716.4 Da PI: 6.9134

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT1G51140.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	37.7	3.7e-12	316	361	6	55
HHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 6 nerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 +++Er RR +i +++ +L++l+P+ ++ + a++L +Av+YIk+Lq AT1G51140.1 316 SIAERVRRTKISERMRKLQDLVPNM----DTQTNTADMLDLAVQYIKDLQ 361 689*******************7....79****************9 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50888	15.072	310	360	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamily	SSF47459	2.49E-16	313	376	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	7.09E-12	314	365	No hit	No description
Gene3D	G3DSA:4.10.280.10	9.4E-16	315	372	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	4.0E-9	316	361	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	1.4E-13	316	366	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0010119	Biological Process	regulation of stomatal movement
GO:0042335	Biological Process	cuticle development
GO:0045944	Biological Process	positive regulation of transcription from RNA polymerase II promoter
GO:0048573	Biological Process	photoperiodism, flowering
GO:0005634	Cellular Component	nucleus
GO:0001046	Molecular Function	core promoter sequence-specific DNA binding
GO:0001228	Molecular Function	transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0005515	Molecular Function	protein binding
GO:0046983	Molecular Function	protein dimerization activity

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0008019	anatomy	leaf lamina base
PO:0009005	anatomy	root
PO:0009006	anatomy	shoot system
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0025281	anatomy	pollen
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007064	developmental stage	LP.12 twelve leaves visible stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 379 aa Download sequence Send to blast
MESEFQQHHF LLHDHQHQRP RNSGLIRYQS APSSYFSSFG ESIEEFLDRP TSPETERILS 60 GFLQTTDTSD NVDSFLHHTF NSDGTEKKPP EVKTEDEDAE IPVTATATAM EVVVSGDGEI 120 SVNPEVSIGY VASVSRNKRP REKDDRTPVN NLARHNSSPA GLFSSIDVET AYAAVMKSMG 180 GFGGSNVMST SNTEASSLTP RSKLLPPTSR AMSPISEVDV KPGFSSRLPP RTLSGGFNRS 240 FGNEGSASSK LTALARTQSG GLDQYKTKDE DSASRRPPLA HHMSLPKSLS DIEQLLSDSI 300 PCKIRAKRGC ATHPRSIAER VRRTKISERM RKLQDLVPNM DTQTNTADML DLAVQYIKDL 360 QEQVKALEES RARCRCSSA

Sequence ? help Back to Top

Protein Sequence Length: 379 aa Download sequence Send to blast

MESEFQQHHF LLHDHQHQRP RNSGLIRYQS APSSYFSSFG ESIEEFLDRP TSPETERILS  60
GFLQTTDTSD NVDSFLHHTF NSDGTEKKPP EVKTEDEDAE IPVTATATAM EVVVSGDGEI  120
SVNPEVSIGY VASVSRNKRP REKDDRTPVN NLARHNSSPA GLFSSIDVET AYAAVMKSMG  180
GFGGSNVMST SNTEASSLTP RSKLLPPTSR AMSPISEVDV KPGFSSRLPP RTLSGGFNRS  240
FGNEGSASSK LTALARTQSG GLDQYKTKDE DSASRRPPLA HHMSLPKSLS DIEQLLSDSI  300
PCKIRAKRGC ATHPRSIAER VRRTKISERM RKLQDLVPNM DTQTNTADML DLAVQYIKDL  360
QEQVKALEES RARCRCSSA

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.19155	0.0	leaf\| vegetative tissue

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	30694905	0.0
Genevisible	245748_at	0.0
Expression Atlas	AT1G51140	-
AtGenExpress	AT1G51140	-
ATTED-II	AT1G51140	-

Function -- GeneRIF ? help Back to Top
These results suggest that bHLH122 functions as a positive regulator of drought, sodium chloride and osmotic signaling. [PMID: 24261563] Phosphorylation results in the monomerization of AKS1 multimers and inhibits AKS1 binding to DNA. AKS1 forms homo-multimers which dissociate following phosphorylation. [PMID: 27227462] AKS1 provides a link between the signaling complex and abscisic Acid-responsive genes.SnRK2.6 phosphorylates AKS1 in guard cells. [PMID: 28438792]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00195	DAP	27203113	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT1G51140.1

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT1G51140

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AY034941	0.0	AY034941.1 Arabidopsis thaliana unknown protein (At1g51140) mRNA, complete cds.
GenBank	AY063120	0.0	AY063120.1 Arabidopsis thaliana unknown protein (At1g51140) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_564583.1	0.0	basic helix-loop-helix (bHLH) DNA-binding superfamily protein
Swissprot	Q9C690	0.0	BH122_ARATH; Transcription factor bHLH122
TrEMBL	A0A178WHN7	0.0	A0A178WHN7_ARATH; FBH3
STRING	AT1G51140.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM10547	26	32
Representative plant	OGRP511	15	80

Link Out ? help Back to Top
Phytozome	AT1G51140.1
Entrez Gene	841537
iHOP	AT1G51140
wikigenes	AT1G51140

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Heim MA, et al. The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity. Mol. Biol. Evol., 2003. 20(5): p. 735-47 [PMID:12679534] Toledo-Ortiz G,Huq E,Quail PH The Arabidopsis basic/helix-loop-helix transcription factor family. Plant Cell, 2003. 15(8): p. 1749-70 [PMID:12897250] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Bailey PC, et al. Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana. Plant Cell, 2003. 15(11): p. 2497-502 [PMID:14600211] Rizhsky L, et al. When defense pathways collide. The response of Arabidopsis to a combination of drought and heat stress. Plant Physiol., 2004. 134(4): p. 1683-96 [PMID:15047901] Xin Z,Mandaokar A,Chen J,Last RL,Browse J Arabidopsis ESK1 encodes a novel regulator of freezing tolerance. Plant J., 2007. 49(5): p. 786-99 [PMID:17316173] Peng M,Bi YM,Zhu T,Rothstein SJ Genome-wide analysis of Arabidopsis responsive transcriptome to nitrogen limitation and its regulation by the ubiquitin ligase gene NLA. Plant Mol. Biol., 2007. 65(6): p. 775-97 [PMID:17885809] Ascencio-Ib Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection. Plant Physiol., 2008. 148(1): p. 436-54 [PMID:18650403] Reiland S, et al. Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks. Plant Physiol., 2009. 150(2): p. 889-903 [PMID:19376835] Ito S, et al. FLOWERING BHLH transcriptional activators control expression of the photoperiodic flowering regulator CONSTANS in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A., 2012. 109(9): p. 3582-7 [PMID:22334645] Efroni I, et al. Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses. Dev. Cell, 2013. 24(4): p. 438-45 [PMID:23449474] Wang P, et al. Quantitative phosphoproteomics identifies SnRK2 protein kinase substrates and reveals the effectors of abscisic acid action. Proc. Natl. Acad. Sci. U.S.A., 2013. 110(27): p. 11205-10 [PMID:23776212] Takahashi Y, et al. bHLH transcription factors that facilitate K⁺ uptake during stomatal opening are repressed by abscisic acid through phosphorylation. Sci Signal, 2013. 6(280): p. ra48 [PMID:23779086] Yang Z, et al. Stable isotope metabolic labeling-based quantitative phosphoproteomic analysis of Arabidopsis mutants reveals ethylene-regulated time-dependent phosphoproteins and putative substrates of constitutive triple response 1 kinase. Mol. Cell Proteomics, 2013. 12(12): p. 3559-82 [PMID:24043427] Liu W, et al. bHLH122 is important for drought and osmotic stress resistance in Arabidopsis and in the repression of ABA catabolism. New Phytol., 2014. 201(4): p. 1192-204 [PMID:24261563] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Li S, et al. CFLAP1 and CFLAP2 Are Two bHLH Transcription Factors Participating in Synergistic Regulation of AtCFL1-Mediated Cuticle Development in Arabidopsis. PLoS Genet., 2016. 12(1): p. e1005744 [PMID:26745719] Takahashi Y,Kinoshita T,Matsumoto M,Shimazaki K Inhibition of the Arabidopsis bHLH transcription factor by monomerization through abscisic acid-induced phosphorylation. Plant J., 2016. 87(6): p. 559-67 [PMID:27227462] Song L, et al. A transcription factor hierarchy defines an environmental stress response network. Science, 2017. [PMID:27811239] Takahashi Y,Ebisu Y,Shimazaki KI Reconstitution of Abscisic Acid Signaling from the Receptor to DNA via bHLH Transcription Factors. Plant Physiol., 2017. 174(2): p. 815-822 [PMID:28438792]