PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT1G49720.1

Common Name

ABF1, AtABF1, BZIP35, F14J22.7

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

bZIP

Protein Properties

Length: 392aa MW: 42727.6 Da PI: 9.5497

Description

abscisic acid responsive element-binding factor 1

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT1G49720.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	bZIP_1	44.8	2.7e-14	313	364	5	56
CHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH CS bZIP_1 5 krerrkqkNReAArrsRqRKkaeieeLeekvkeLeaeNkaLkkeleelkkev 56 +r++r++kNRe+A rsR+RK+a++ eLe ++++L+ N++L+k+ e+ k+ AT1G49720.1 313 RRQKRMIKNRESAARSRARKQAYTLELEAEIESLKLVNQDLQKKQAEIMKTH 364 79***************************************98877776654 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SMART	SM00338	4.7E-14	309	374	IPR004827	Basic-leucine zipper domain
PROSITE profile	PS50217	10.117	311	362	IPR004827	Basic-leucine zipper domain
CDD	cd14707	9.70E-23	313	363	No hit	No description
Gene3D	G3DSA:1.20.5.170	4.0E-13	313	369	No hit	No description
SuperFamily	SSF57959	2.46E-10	313	363	No hit	No description
Pfam	PF00170	4.2E-12	313	369	IPR004827	Basic-leucine zipper domain
PROSITE pattern	PS00036	0	316	331	IPR004827	Basic-leucine zipper domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0009737	Biological Process	response to abscisic acid
GO:0009738	Biological Process	abscisic acid-activated signaling pathway
GO:0045893	Biological Process	positive regulation of transcription, DNA-templated
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0005515	Molecular Function	protein binding
GO:0043565	Molecular Function	sequence-specific DNA binding
GO:0044212	Molecular Function	transcription regulatory region DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000005	anatomy	cultured plant cell
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0008019	anatomy	leaf lamina base
PO:0009005	anatomy	root
PO:0009006	anatomy	shoot system
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0025281	anatomy	pollen
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 392 aa Download sequence Send to blast
MGTHIDINNL GGDTSRGNES KPLARQSSLY SLTFDELQST LGEPGKDFGS MNMDELLKNI 60 WTAEDTQAFM TTTSSVAAPG PSGFVPGGNG LQRQGSLTLP RTLSQKTVDE VWKYLNSKEG 120 SNGNTGTDAL ERQQTLGEMT LEDFLLRAGV VKEDNTQQNE NSSSGFYANN GAAGLEFGFG 180 QPNQNSISFN GNNSSMIMNQ APGLGLKVGG TMQQQQQPHQ QQLQQPHQRL PPTIFPKQAN 240 VTFAAPVNMV NRGLFETSAD GPANSNMGGA GGTVTATSPG TSSAENNTWS SPVPYVFGRG 300 RRSNTGLEKV VERRQKRMIK NRESAARSRA RKQAYTLELE AEIESLKLVN QDLQKKQAEI 360 MKTHNSELKE FSKQPPLLAK RQCLRRTLTG PW

Sequence ? help Back to Top

Protein Sequence Length: 392 aa Download sequence Send to blast

MGTHIDINNL GGDTSRGNES KPLARQSSLY SLTFDELQST LGEPGKDFGS MNMDELLKNI  60
WTAEDTQAFM TTTSSVAAPG PSGFVPGGNG LQRQGSLTLP RTLSQKTVDE VWKYLNSKEG  120
SNGNTGTDAL ERQQTLGEMT LEDFLLRAGV VKEDNTQQNE NSSSGFYANN GAAGLEFGFG  180
QPNQNSISFN GNNSSMIMNQ APGLGLKVGG TMQQQQQPHQ QQLQQPHQRL PPTIFPKQAN  240
VTFAAPVNMV NRGLFETSAD GPANSNMGGA GGTVTATSPG TSSAENNTWS SPVPYVFGRG  300
RRSNTGLEKV VERRQKRMIK NRESAARSRA RKQAYTLELE AEIESLKLVN QDLQKKQAEI  360
MKTHNSELKE FSKQPPLLAK RQCLRRTLTG PW

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.46187	0.0	flower\| leaf\| seed

Expression -- Microarray ? help Back to Top
Source	ID	E-value
Genevisible	261613_at	0.0
Expression Atlas	AT1G49720	-
AtGenExpress	AT1G49720	-
ATTED-II	AT1G49720	-

Functional Description ? help Back to Top
Source	Description
TAIR	Identified as a protein that binds to abscisic acid response elements. May mediate transcriptional regulation of ABA responses.
UniProt	Binds to the ABA-responsive element (ABRE). Could participate in abscisic acid-regulated gene expression.

Function -- GeneRIF ? help Back to Top
These studies on abscisic acid response element binding factor 1 (ABF1) gene in Arabidopsis demonstrate that it is required for seedling establishment during winter. [PMID: 21181499] ABF1 and ABF3 abundance is affected by ABA and KEG [PMID: 23742014] The four AREB/ABFs are the predominant transcription factors downstream of SRK2D/E/I in abscisic acid (ABA) signalling in response to osmotic stress during vegetative growth. [PMID: 24738645] the role of ABF1/3/4 in det1 germination phenotypes [PMID: 29680877]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00087	SELEX	26531826	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT1G49720.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Up-regulated by abscisic acid (ABA) and cold. {ECO:0000269\|PubMed:10636868}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- Hormone ? help Back to Top
Source	Hormone
AHD	abscisic acid

Interaction ? help Back to Top
Source	Intact With
BioGRID	AT1G49720
IntAct	Search Q9M7Q5

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT1G49720

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AF093544	0.0	AF093544.1 Arabidopsis thaliana clone 1 abscisic acid responsive elements-binding factor (ABRE) mRNA, complete cds.
GenBank	AK226983	0.0	AK226983.1 Arabidopsis thaliana mRNA for abscisic acid responsive elements-binding factor, complete cds, clone: RAFL09-41-C22.
GenBank	BT008575	0.0	BT008575.1 Arabidopsis thaliana clone U19471 putative abscisic acid responsive elements-binding factor (At1g49720) mRNA, complete cds.
GenBank	BT008647	0.0	BT008647.1 Arabidopsis thaliana clone RAFL09-77-C02 (R19471) putative abscisic acid responsive elements-binding factor (At1g49720) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_001322564.1	0.0	abscisic acid responsive element-binding factor 1
Refseq	NP_564551.1	0.0	abscisic acid responsive element-binding factor 1
Swissprot	Q9M7Q5	0.0	AI5L4_ARATH; ABSCISIC ACID-INSENSITIVE 5-like protein 4
TrEMBL	A0A178WH34	0.0	A0A178WH34_ARATH; AtABF1
STRING	AT1G49720.2	0.0	(Arabidopsis thaliana)

Link Out ? help Back to Top
Phytozome	AT1G49720.1
Entrez Gene	841395
iHOP	AT1G49720
wikigenes	AT1G49720

Publications ? help Back to Top
Choi H,Hong J,Ha J,Kang J,Kim SY ABFs, a family of ABA-responsive element binding factors. J. Biol. Chem., 2000. 275(3): p. 1723-30 [PMID:10636868] Finkelstein RR,Lynch TJ The Arabidopsis abscisic acid response gene ABI5 encodes a basic leucine zipper transcription factor. Plant Cell, 2000. 12(4): p. 599-609 [PMID:10760247] Riechmann JL,Ratcliffe OJ A genomic perspective on plant transcription factors. Curr. Opin. Plant Biol., 2000. 3(5): p. 423-34 [PMID:11019812] Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Jakoby M, et al. bZIP transcription factors in Arabidopsis. Trends Plant Sci., 2002. 7(3): p. 106-11 [PMID:11906833] Finkelstein RR,Gampala SS,Rock CD Abscisic acid signaling in seeds and seedlings. Plant Cell, 2002. 14 Suppl: p. S15-45 [PMID:12045268] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Finkelstein R,Gampala SS,Lynch TJ,Thomas TL,Rock CD Redundant and distinct functions of the ABA response loci ABA-INSENSITIVE(ABI)5 and ABRE-BINDING FACTOR (ABF)3. Plant Mol. Biol., 2005. 59(2): p. 253-67 [PMID:16247556] Choi HI, et al. Arabidopsis calcium-dependent protein kinase AtCPK32 interacts with ABF4, a transcriptional regulator of abscisic acid-responsive gene expression, and modulates its activity. Plant Physiol., 2005. 139(4): p. 1750-61 [PMID:16299177] Fujii H,Verslues PE,Zhu JK Identification of two protein kinases required for abscisic acid regulation of seed germination, root growth, and gene expression in Arabidopsis. Plant Cell, 2007. 19(2): p. 485-94 [PMID:17307925] Zhu SY, et al. Two calcium-dependent protein kinases, CPK4 and CPK11, regulate abscisic acid signal transduction in Arabidopsis. Plant Cell, 2007. 19(10): p. 3019-36 [PMID:17921317] Garcia ME,Lynch T,Peeters J,Snowden C,Finkelstein R A small plant-specific protein family of ABI five binding proteins (AFPs) regulates stress response in germinating Arabidopsis seeds and seedlings. Plant Mol. Biol., 2008. 67(6): p. 643-58 [PMID:18484180] Kline KG,Barrett-Wilt GA,Sussman MR In planta changes in protein phosphorylation induced by the plant hormone abscisic acid. Proc. Natl. Acad. Sci. U.S.A., 2010. 107(36): p. 15986-91 [PMID:20733066] Sharma PD,Singh N,Ahuja PS,Reddy TV Abscisic acid response element binding factor 1 is required for establishment of Arabidopsis seedlings during winter. Mol. Biol. Rep., 2011. 38(8): p. 5147-59 [PMID:21181499] Lalonde S, et al. A membrane protein/signaling protein interaction network for Arabidopsis version AMPv2. Front Physiol, 2010. 1: p. 24 [PMID:21423366] Zhao R, et al. The Arabidopsis Ca(2+) -dependent protein kinase CPK12 negatively regulates abscisic acid signaling in seed germination and post-germination growth. New Phytol., 2011. 192(1): p. 61-73 [PMID:21692804] Gaudinier A, et al. Enhanced Y1H assays for Arabidopsis. Nat. Methods, 2011. 8(12): p. 1053-5 [PMID:22037706] Zhao R,Wang XF,Zhang DP CPK12: A Ca2+-dependent protein kinase balancer in abscisic acid signaling. Plant Signal Behav, 2011. 6(11): p. 1687-90 [PMID:22041934] Lynch T,Erickson BJ,Finkelstein RR Direct interactions of ABA-insensitive(ABI)-clade protein phosphatase(PP)2Cs with calcium-dependent protein kinases and ABA response element-binding bZIPs may contribute to turning off ABA response. Plant Mol. Biol., 2012. 80(6): p. 647-58 [PMID:23007729] Efroni I, et al. Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses. Dev. Cell, 2013. 24(4): p. 438-45 [PMID:23449474] Chen YT,Liu H,Stone S,Callis J ABA and the ubiquitin E3 ligase KEEP ON GOING affect proteolysis of the Arabidopsis thaliana transcription factors ABF1 and ABF3. Plant J., 2013. 75(6): p. 965-76 [PMID:23742014] Lim S, et al. ABA-insensitive3, ABA-insensitive5, and DELLAs Interact to activate the expression of SOMNUS and other high-temperature-inducible genes in imbibed seeds in Arabidopsis. Plant Cell, 2013. 25(12): p. 4863-78 [PMID:24326588] Yoshida T, et al. Four Arabidopsis AREB/ABF transcription factors function predominantly in gene expression downstream of SnRK2 kinases in abscisic acid signalling in response to osmotic stress. Plant Cell Environ., 2015. 38(1): p. 35-49 [PMID:24738645] Fernando VCD,Al Khateeb W,Belmonte MF,Schroeder DF Role of Arabidopsis ABF1/3/4 during det1 germination in salt and osmotic stress conditions. Plant Mol. Biol., 2018. 97(1-2): p. 149-163 [PMID:29680877]