PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT1G46768.1

Common Name

ERF006, F2G19.32, RAP2-1, RAP2.1

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

ERF

Protein Properties

Length: 153aa MW: 17240.6 Da PI: 9.8152

Description

related to AP2 1

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT1G46768.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	AP2	54.3	3.4e-17	30	79	2	55
AP2 2 gykGVrwdkkrgrWvAeIrdpsengkrkrfslgkfgtaeeAakaaiaarkkleg 55 y+G+r++k +g+WvAeIr+p +kr r +lg++ t Aa+a++ a +l+g AT1G46768.1 30 PYRGIRRRK-WGKWVAEIREP---NKRSRLWLGSYTTDIAAARAYDVAVFYLRG 79 69**998.******9...336***********************98 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS51032	23.011	30	87	IPR001471	AP2/ERF domain
SuperFamily	SSF54171	1.24E-20	30	88	IPR016177	DNA-binding domain
SMART	SM00380	3.0E-35	30	93	IPR001471	AP2/ERF domain
PRINTS	PR00367	3.8E-12	31	42	IPR001471	AP2/ERF domain
Gene3D	G3DSA:3.30.730.10	2.7E-30	31	88	IPR001471	AP2/ERF domain
Pfam	PF00847	7.1E-12	31	79	IPR001471	AP2/ERF domain
CDD	cd00018	4.18E-26	31	89	No hit	No description
PRINTS	PR00367	3.8E-12	69	89	IPR001471	AP2/ERF domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0009409	Biological Process	response to cold
GO:0009414	Biological Process	response to water deprivation
GO:0009873	Biological Process	ethylene-activated signaling pathway
GO:0042538	Biological Process	hyperosmotic salinity response
GO:0045893	Biological Process	positive regulation of transcription, DNA-templated
GO:0005634	Cellular Component	nucleus
GO:0001078	Molecular Function	transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
GO:0003677	Molecular Function	DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000293	anatomy	guard cell
PO:0009030	anatomy	carpel
PO:0009047	anatomy	stem
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 153 aa Download sequence Send to blast
MEREQEESTM RKRRQPPQEE VPNHVATRKP YRGIRRRKWG KWVAEIREPN KRSRLWLGSY 60 TTDIAAARAY DVAVFYLRGP SARLNFPDLL LQEEDHLSAA TTADMPAALI REKAAEVGAR 120 VDALLASAAP SMAHSTPPVI KPDLNQIPES GDI

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
5wx9_A	9e-15	26	87	10	72	Ethylene-responsive transcription factor ERF096
Search in ModeBase

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	9	14	MRKRRQ

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.28676	0.0	inflorescence\| seed

Expression -- Microarray ? help Back to Top
Source	ID	E-value
Genevisible	245807_at	0.0
Expression Atlas	AT1G46768	-
AtGenExpress	AT1G46768	-
ATTED-II	AT1G46768	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed at low levels in flowers, leaves, and stems, and at very low levels in roots. {ECO:0000269\|PubMed:9192694}.

Functional Description ? help Back to Top
Source	Description
TAIR	encodes a member of the DREB subfamily A-5 of ERF/AP2 transcription factor family (RAP2.1). The protein contains one AP2 domain. There are 16 members in this subfamily including RAP2.9 and RAP2.10.
UniProt	Probably acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). {ECO:0000250}.

Function -- GeneRIF ? help Back to Top
RAP2.1 is a DREB-type, EAR-motif-containing transcriptional repressor that negatively regulates plant responses to cold and drought stresses. [PMID: 20230648]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00188	DAP	27203113	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT1G46768.1

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source	Upstream Regulator (A: Activate/R: Repress)
ATRM	AT1G46768 (R), AT4G25470 (A), AT4G25480 (A), AT4G25490 (A)

Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source	Target Gene (A: Activate/R: Repress)
ATRM	AT1G46768(R), AT2G42540(R), AT5G52310(R)

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT1G46768

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AC083835	0.0	AC083835.5 Arabidopsis thaliana chromosome 1 BAC F2G19 genomic sequence, complete sequence.
GenBank	AY086838	0.0	AY086838.1 Arabidopsis thaliana clone 28451 mRNA, complete sequence.
GenBank	BT024691	0.0	BT024691.1 Arabidopsis thaliana At1g46768 mRNA, complete cds.
GenBank	CP002684	0.0	CP002684.1 Arabidopsis thaliana chromosome 1 sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_001322276.1	1e-108	related to AP2 1
Refseq	NP_564496.1	1e-108	related to AP2 1
Swissprot	Q8LC30	1e-110	RAP21_ARATH; Ethylene-responsive transcription factor RAP2-1
TrEMBL	A0A178WAW3	1e-107	A0A178WAW3_ARATH; RAP2.1
STRING	AT1G46768.1	1e-108	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM10	28	1650
Representative plant	OGRP6	16	1718

Link Out ? help Back to Top
Phytozome	AT1G46768.1
Entrez Gene	841117
iHOP	AT1G46768
wikigenes	AT1G46768

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Maruyama K, et al. Identification of cold-inducible downstream genes of the Arabidopsis DREB1A/CBF3 transcriptional factor using two microarray systems. Plant J., 2004. 38(6): p. 982-93 [PMID:15165189] Zhang JZ,Creelman RA,Zhu JK From laboratory to field. Using information from Arabidopsis to engineer salt, cold, and drought tolerance in crops. Plant Physiol., 2004. 135(2): p. 615-21 [PMID:15173567] Zhang X, et al. Freezing-sensitive tomato has a functional CBF cold response pathway, but a CBF regulon that differs from that of freezing-tolerant Arabidopsis. Plant J., 2004. 39(6): p. 905-19 [PMID:15341633] Vogel JT,Zarka DG,Van Buskirk HA,Fowler SG,Thomashow MF Roles of the CBF2 and ZAT12 transcription factors in configuring the low temperature transcriptome of Arabidopsis. Plant J., 2005. 41(2): p. 195-211 [PMID:15634197] Hannah MA,Heyer AG,Hincha DK A global survey of gene regulation during cold acclimation in Arabidopsis thaliana. PLoS Genet., 2005. 1(2): p. e26 [PMID:16121258] Kim S,Soltis PS,Wall K,Soltis DE Phylogeny and domain evolution in the APETALA2-like gene family. Mol. Biol. Evol., 2006. 23(1): p. 107-20 [PMID:16151182] Nakano T,Suzuki K,Fujimura T,Shinshi H Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol., 2006. 140(2): p. 411-32 [PMID:16407444] Oono Y, et al. Monitoring expression profiles of Arabidopsis genes during cold acclimation and deacclimation using DNA microarrays. Funct. Integr. Genomics, 2006. 6(3): p. 212-34 [PMID:16463051] Xiong Y,Fei SZ Functional and phylogenetic analysis of a DREB/CBF-like gene in perennial ryegrass (Lolium perenne L.). Planta, 2006. 224(4): p. 878-88 [PMID:16614820] Benedict C,Geisler M,Trygg J,Huner N,Hurry V Consensus by democracy. Using meta-analyses of microarray and genomic data to model the cold acclimation signaling pathway in Arabidopsis. Plant Physiol., 2006. 141(4): p. 1219-32 [PMID:16896234] Willems G, et al. The genetic basis of zinc tolerance in the metallophyte Arabidopsis halleri ssp. halleri (Brassicaceae): an analysis of quantitative trait loci. Genetics, 2007. 176(1): p. 659-74 [PMID:17409091] Welling A,Palva ET Involvement of CBF transcription factors in winter hardiness in birch. Plant Physiol., 2008. 147(3): p. 1199-211 [PMID:18467468] Tsutsui T, et al. DEAR1, a transcriptional repressor of DREB protein that mediates plant defense and freezing stress responses in Arabidopsis. J. Plant Res., 2009. 122(6): p. 633-43 [PMID:19618250] Dong CJ,Liu JY The Arabidopsis EAR-motif-containing protein RAP2.1 functions as an active transcriptional repressor to keep stress responses under tight control. BMC Plant Biol., 2010. 10: p. 47 [PMID:20230648] Efroni I, et al. Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses. Dev. Cell, 2013. 24(4): p. 438-45 [PMID:23449474] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178] Okamuro JK,Caster B,Villarroel R,Van Montagu M,Jofuku KD The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A., 1997. 94(13): p. 7076-81 [PMID:9192694]