PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT1G15360.1

Common Name

AtSHN1, ERF001, F9L1.31, SHN1, WIN1

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

ERF

Protein Properties

Length: 199aa MW: 21696.3 Da PI: 7.4449

Description

ERF family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT1G15360.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	AP2	62.2	1.1e-19	5	53	1	53
AP2 1 sgykGVrwdkkrgrWvAeIrdpsengkr..krfslgkfgtaeeAakaaiaarkkl 53 ++++GVr+++ +g+WvAeIr+p +r++lg+f taeeAa+a+++a+ + AT1G15360.1 5 KKFRGVRQRH-WGSWVAEIRHP-----LlkRRIWLGTFETAEEAARAYDEAAVLM 53 59*****9.******8.....334*******************9765 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
CDD	cd00018	7.48E-34	5	63	No hit	No description
SMART	SM00380	4.3E-40	6	69	IPR001471	AP2/ERF domain
PROSITE profile	PS51032	22.155	6	63	IPR001471	AP2/ERF domain
Pfam	PF00847	1.6E-12	6	50	IPR001471	AP2/ERF domain
Gene3D	G3DSA:3.30.730.10	4.4E-33	6	64	IPR001471	AP2/ERF domain
SuperFamily	SSF54171	3.2E-21	6	65	IPR016177	DNA-binding domain
PRINTS	PR00367	1.3E-10	7	18	IPR001471	AP2/ERF domain
PRINTS	PR00367	1.3E-10	29	45	IPR001471	AP2/ERF domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009414	Biological Process	response to water deprivation
GO:0009873	Biological Process	ethylene-activated signaling pathway
GO:0010143	Biological Process	cutin biosynthetic process
GO:0010166	Biological Process	wax metabolic process
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000014	anatomy	rosette leaf
PO:0004707	anatomy	fruit dehiscence zone
PO:0004711	anatomy	axillary inflorescence bud
PO:0009001	anatomy	fruit
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0025022	anatomy	collective leaf structure
PO:0025281	anatomy	pollen
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 199 aa Download sequence Send to blast
MVQTKKFRGV RQRHWGSWVA EIRHPLLKRR IWLGTFETAE EAARAYDEAA VLMSGRNAKT 60 NFPLNNNNTG ETSEGKTDIS ASSTMSSSTS SSSLSSILSA KLRKCCKSPS PSLTCLRLDT 120 ASSHIGVWQK RAGSKSDSSW VMTVELGPAS SSQETTSKAS QDAILAPTTE VEIGGSREEV 180 LDEEEKVALQ MIEELLNTN

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2gcc_A	8e-18	3	67	2	67	ATERF1
3gcc_A	8e-18	3	67	2	67	ATERF1
Search in ModeBase

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.11422	0.0	inflorescence

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	145335735	0.0
Genevisible	262595_at	0.0
Expression Atlas	AT1G15360	-
AtGenExpress	AT1G15360	-
ATTED-II	AT1G15360	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	DEVELOPMENTAL STAGE: Detected in sepals of very young closed buds. Later, expressed in sepals and petals veins and epidermis, as well as in developing gynoecium but not in stamens. At anthesis, confined to the gynoecium, commenced in the anther, and slightly expressed in the anther filament. When petals and sepals withered, strong expression at the bottom of the silique, in the abscission zone, and in the pedicel region below it. At silique maturity, detected in the same region but only at the nectaries. {ECO:0000269\|PubMed:15319479}.
Uniprot	TISSUE SPECIFICITY: Expressed in aerial organs, mostly in flowers, and in roots. Also observed at the branch points of pedicels of most young flowers, and in a patchy pattern in roots of mature plants and very young leaves in the rosette, including support cells of their trichomes. {ECO:0000269\|PubMed:15070782, ECO:0000269\|PubMed:15319479}.

Functional Description ? help Back to Top
Source	Description
TAIR	Encodes a member of the ERF (ethylene response factor) subfamily B-6 of ERF/AP2 transcription factor family. The protein contains one AP2 domain. There are 12 members in this subfamily including RAP2.11. This gene is involved in wax biosynthesis. Over-expression of the gene results in glossy leaf phenotype and increased drought tolerance. Two closely related genes, AT5G25390 and AT5G11190 have similar phenotypes when over-expressed. Strong expression levels in flowers. Binds to the promoter of LACS2.
UniProt	Promotes cuticle formation by inducing the expression of enzymes involved in wax biosynthesis (PubMed:15070782, PubMed:15319479). Confers drought resistance (PubMed:15319479). Acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). {ECO:0000250, ECO:0000269\|PubMed:15070782, ECO:0000269\|PubMed:15319479}.

Function -- GeneRIF ? help Back to Top
At least one of the cutin pathway genes, which encodes long-chain acyl-CoA synthetase LACS2, is likely to be directly targeted by WIN1. [PMID: 17449808] Work identified WIN1, a putative acetylornithine transaminase, as Pseudomonas syringae HopW1-1-interacting plant proteins. [PMID: 18266921] Silencing SHINE clade genes (SHN1/SHN2/SHN3) reduces flower cutin load and modifies petal cell wall structure. [PMID: 21637781] when expression of the WIN1/SHN1 gene was induced there was increased expression of genes involved in wax development... [PMID: 22078383] Data indicate that the MIXTA-like MYB transcription factors MYB106 and MYB16, which regulate epidermal cell morphology, also regulate cuticle development coordinately with WIN1/SHN1. [PMID: 23709630] Overexpression of AtSHN1/WIN1 provokes unique defense responses. [PMID: 23922943]

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT1G15360.1

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source	Target Gene (A: Activate/R: Repress)
ATRM	AT1G01120(A), AT1G02205(A), AT4G24510(A)

Regulation -- Hormone ? help Back to Top
Source	Hormone
AHD	ethylene

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT1G15360

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AK117941	0.0	AK117941.1 Arabidopsis thaliana At1g15360 mRNA for putative ethylene responsive element, complete cds, clone: RAFL19-13-G20.
GenBank	AY378101	0.0	AY378101.1 Arabidopsis thaliana transcription factor wax inducer 1 (WIN1) mRNA, complete cds.
GenBank	BT005220	0.0	BT005220.1 Arabidopsis thaliana At1g15360 mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_001322723.1	1e-144	Integrase-type DNA-binding superfamily protein
Refseq	NP_172988.1	1e-144	Integrase-type DNA-binding superfamily protein
Swissprot	Q9XI33	1e-145	WIN1_ARATH; Ethylene-responsive transcription factor WIN1
TrEMBL	A0A178WNC4	1e-142	A0A178WNC4_ARATH; WIN1
STRING	AT1G15360.1	1e-143	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM834	28	116
Representative plant	OGRP6	16	1718

Link Out ? help Back to Top
Phytozome	AT1G15360.1
Entrez Gene	838105
iHOP	AT1G15360
wikigenes	AT1G15360

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Seki M, et al. Functional annotation of a full-length Arabidopsis cDNA collection. Science, 2002. 296(5565): p. 141-5 [PMID:11910074] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Hu W,Wang Y,Bowers C,Ma H Isolation, sequence analysis, and expression studies of florally expressed cDNAs in Arabidopsis. Plant Mol. Biol., 2003. 53(4): p. 545-63 [PMID:15010618] Broun P,Poindexter P,Osborne E,Jiang CZ,Riechmann JL WIN1, a transcriptional activator of epidermal wax accumulation in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A., 2004. 101(13): p. 4706-11 [PMID:15070782] Aharoni A, et al. The SHINE clade of AP2 domain transcription factors activates wax biosynthesis, alters cuticle properties, and confers drought tolerance when overexpressed in Arabidopsis. Plant Cell, 2004. 16(9): p. 2463-80 [PMID:15319479] Nakano T,Suzuki K,Fujimura T,Shinshi H Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol., 2006. 140(2): p. 411-32 [PMID:16407444] Kurdyukov S, et al. The epidermis-specific extracellular BODYGUARD controls cuticle development and morphogenesis in Arabidopsis. Plant Cell, 2006. 18(2): p. 321-39 [PMID:16415209] Mandaokar A, et al. Transcriptional regulators of stamen development in Arabidopsis identified by transcriptional profiling. Plant J., 2006. 46(6): p. 984-1008 [PMID:16805732] Cao D,Cheng H,Wu W,Soo HM,Peng J Gibberellin mobilizes distinct DELLA-dependent transcriptomes to regulate seed germination and floral development in Arabidopsis. Plant Physiol., 2006. 142(2): p. 509-25 [PMID:16920880] Kannangara R, et al. The transcription factor WIN1/SHN1 regulates Cutin biosynthesis in Arabidopsis thaliana. Plant Cell, 2007. 19(4): p. 1278-94 [PMID:17449808] Lee MW,Jelenska J,Greenberg JT Arabidopsis proteins important for modulating defense responses to Pseudomonas syringae that secrete HopW1-1. Plant J., 2008. 54(3): p. 452-65 [PMID:18266921] Taketa S, et al. Barley grain with adhering hulls is controlled by an ERF family transcription factor gene regulating a lipid biosynthesis pathway. Proc. Natl. Acad. Sci. U.S.A., 2008. 105(10): p. 4062-7 [PMID:18316719] Kamigaki A,Kondo M,Mano S,Hayashi M,Nishimura M Suppression of peroxisome biogenesis factor 10 reduces cuticular wax accumulation by disrupting the ER network in Arabidopsis thaliana. Plant Cell Physiol., 2009. 50(12): p. 2034-46 [PMID:19892830] Shi JX, et al. SHINE transcription factors act redundantly to pattern the archetypal surface of Arabidopsis flower organs. PLoS Genet., 2011. 7(5): p. e1001388 [PMID:21637781] Qin F, et al. SPINDLY, a negative regulator of gibberellic acid signaling, is involved in the plant abiotic stress response. Plant Physiol., 2011. 157(4): p. 1900-13 [PMID:22013217] Yang J,Isabel Ordiz M,Jaworski JG,Beachy RN Induced accumulation of cuticular waxes enhances drought tolerance in Arabidopsis by changes in development of stomata. Plant Physiol. Biochem., 2011. 49(12): p. 1448-55 [PMID:22078383] Wang Y, et al. An ethylene response factor OsWR1 responsive to drought stress transcriptionally activates wax synthesis related genes and increases wax production in rice. Plant Mol. Biol., 2012. 78(3): p. 275-88 [PMID:22130861] Lisso J,Schr NFXL2 modifies cuticle properties in Arabidopsis. Plant Signal Behav, 2012. 7(5): p. 551-5 [PMID:22516817] Li-Beisson Y, et al. Acyl-lipid metabolism. Arabidopsis Book, 2013. 11: p. e0161 [PMID:23505340] Oshima Y, et al. MIXTA-like transcription factors and WAX INDUCER1/SHINE1 coordinately regulate cuticle development in Arabidopsis and Torenia fournieri. Plant Cell, 2013. 25(5): p. 1609-24 [PMID:23709630] Sela D, et al. Overexpression of AtSHN1/WIN1 provokes unique defense responses. PLoS ONE, 2013. 8(7): p. e70146 [PMID:23922943] Al-Abdallat AM,Al-Debei HS,Ayad JY,Hasan S Over-expression of SlSHN1 gene improves drought tolerance by increasing cuticular wax accumulation in tomato. Int J Mol Sci, 2014. 15(11): p. 19499-515 [PMID:25350113] Djemal R,Khoudi H Isolation and molecular characterization of a novel WIN1/SHN1 ethylene-responsive transcription factor TdSHN1 from durum wheat (Triticum turgidum. L. subsp. durum). Protoplasma, 2015. 252(6): p. 1461-73 [PMID:25687296] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178] Sadler C, et al. Wax and cutin mutants of Arabidopsis: Quantitative characterization of the cuticular transport barrier in relation to chemical composition. Biochim. Biophys. Acta, 2016. 1861(9 Pt B): p. 1336-1344 [PMID:26965486] Sajeevan RS, et al. Expression of Arabidopsis SHN1 in Indian Mulberry (Morus indica L.) Increases Leaf Surface Wax Content and Reduces Post-harvest Water Loss. Front Plant Sci, 2017. 8: p. 418 [PMID:28421085] Riechmann JL,Meyerowitz EM The AP2/EREBP family of plant transcription factors. Biol. Chem., 1998. 379(6): p. 633-46 [PMID:9687012]