PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Neem_9633_f_1

Organism

Azadirachta indica

Taxonomic ID

124943

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Sapindales; Meliaceae; Azadirachta

Family

WRKY

Protein Properties

Length: 358aa MW: 40116.4 Da PI: 5.2591

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Neem_9633_f_1	genome	NGD	View Nucleic Acid

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	93.4	1.7e-29	131	190	2	58
--SS-EEEEEEE--TT-SS-EEEEEE-ST...T---EEEEEE-SSSTTEEEEEEES--SS CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa...gCpvkkkversaedpkvveitYegeHnh 58 +Dgy+WrKYGqK++ g+++prsYYrCt++ +C+++k+v+rs+edp+++eitY+g+H++ Neem_9633_f_1 131 EDGYSWRKYGQKDILGAKYPRSYYRCTYRntqNCWATKQVQRSDEDPTIFEITYRGKHTC 190 8*************************98899***********************98 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50811	22.492	125	188	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	1.1E-28	126	190	IPR003657	WRKY domain
SuperFamily	SSF118290	1.27E-25	129	190	IPR003657	WRKY domain
SMART	SM00774	7.2E-40	130	192	IPR003657	WRKY domain
Pfam	PF03106	8.4E-27	131	190	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0009751	Biological Process	response to salicylic acid
GO:0009816	Biological Process	defense response to bacterium, incompatible interaction
GO:0010150	Biological Process	leaf senescence
GO:0010193	Biological Process	response to ozone
GO:0010200	Biological Process	response to chitin
GO:0031347	Biological Process	regulation of defense response
GO:0042542	Biological Process	response to hydrogen peroxide
GO:0045893	Biological Process	positive regulation of transcription, DNA-templated
GO:0005634	Cellular Component	nucleus
GO:0009507	Cellular Component	chloroplast
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 358 aa Download sequence Send to blast
MEPGWSWEQK TLISELIEGM EMAKQLRVNL VAASSAETSE ALVQRILSSY EKALLILKWS 60 GSMGQAQGVG ATSGVPESPI SINGSPRSED FDAGFKDNQD QQDSSKKRKT MPRWTDKVKV 120 NFETGLEGPL EDGYSWRKYG QKDILGAKYP RSYYRCTYRN TQNCWATKQV QRSDEDPTIF 180 EITYRGKHTC FHGSQAVPQP ASPEKHEQRL NHHNNNHQLK QSQEIISNSR AGLGMNNECL 240 DNKEIASPFS FPSTPFGCMK GNDQCFPPLM LDENNFSGGF SSSFIPPSQA ESNYYFISPN 300 RMNNFGDVHN LHRSESDLPE LQSANNSAAN SPILNLDFSL DPAEIDPNFP FDPAGFSF

Sequence ? help Back to Top

Protein Sequence Length: 358 aa Download sequence Send to blast

MEPGWSWEQK TLISELIEGM EMAKQLRVNL VAASSAETSE ALVQRILSSY EKALLILKWS  60
GSMGQAQGVG ATSGVPESPI SINGSPRSED FDAGFKDNQD QQDSSKKRKT MPRWTDKVKV  120
NFETGLEGPL EDGYSWRKYG QKDILGAKYP RSYYRCTYRN TQNCWATKQV QRSDEDPTIF  180
EITYRGKHTC FHGSQAVPQP ASPEKHEQRL NHHNNNHQLK QSQEIISNSR AGLGMNNECL  240
DNKEIASPFS FPSTPFGCMK GNDQCFPPLM LDENNFSGGF SSSFIPPSQA ESNYYFISPN  300
RMNNFGDVHN LHRSESDLPE LQSANNSAAN SPILNLDFSL DPAEIDPNFP FDPAGFSF

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
6ir8_A	1e-19	130	190	8	68	OsWRKY45
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (PubMed:20409006). May regulate the early events of leaf senescence (PubMed:17369373, PubMed:20409006). Negatively regulates the expression of ESR/ESP (PubMed:20409006). Together with WRKY46 and WRKY70, promotes resistance to P.syringae, probably by enhancing salicylic acid (SA)- dependent genes. Contributes to the suppression of jasmonic acid (MeJA)-induced expression of PDF1.2 (PubMed:22325892). {ECO:0000269\|PubMed:17369373, ECO:0000269\|PubMed:20409006, ECO:0000269\|PubMed:22325892}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By salicylic acid (SA) (PubMed:22268143, PubMed:22325892). Strong accumulation during leaf senescence (PubMed:22268143). Down-regulated by jasmonate. Triggered by P.syringae (PubMed:22325892). {ECO:0000269\|PubMed:11449049, ECO:0000269\|PubMed:11506370, ECO:0000269\|PubMed:17369373, ECO:0000269\|PubMed:22268143, ECO:0000269\|PubMed:22325892}.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_006439376.1	0.0	probable WRKY transcription factor 53
Swissprot	Q9SUP6	1e-71	WRK53_ARATH; Probable WRKY transcription factor 53
TrEMBL	A0A067GMD4	0.0	A0A067GMD4_CITSI; Uncharacterized protein
STRING	XP_006439376.1	0.0	(Citrus clementina)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM1933	27	83

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G23810.1	8e-74	WRKY family protein

Publications ? help Back to Top
Ramírez V,López A,Mauch-Mani B,Gil MJ,Vera P An extracellular subtilase switch for immune priming in Arabidopsis. PLoS Pathog., 2013. 9(6): p. e1003445 [PMID:23818851] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Singh V,Roy S,Singh D,Nandi AK Arabidopsis flowering locus D influences systemic-acquired-resistance- induced expression and histone modifications of WRKY genes. J. Biosci., 2014. 39(1): p. 119-26 [PMID:24499796] Zhang H, et al. Arabidopsis AtERF15 positively regulates immunity against Pseudomonas syringae pv. tomato DC3000 and Botrytis cinerea. Front Plant Sci, 2015. 6: p. 686 [PMID:26388886] Ramírez V,López A,Mauch-Mani B,Gil MJ,Vera P Correction: An Extracellular Subtilase Switch for Immune Priming in Arabidopsis. PLoS Pathog., 2016. 12(11): p. e1006003 [PMID:27806116] Chen X, et al. POWERDRESS interacts with HISTONE DEACETYLASE 9 to promote aging in Arabidopsis. Elife, 2017. [PMID:27873573] Nie P, et al. Induced Systemic Resistance against Botrytis cinerea by Bacillus cereus AR156 through a JA/ET- and NPR1-Dependent Signaling Pathway and Activates PAMP-Triggered Immunity in Arabidopsis. Front Plant Sci, 2017. 8: p. 238 [PMID:28293243] Ren Y,Li Y,Jiang Y,Wu B,Miao Y Phosphorylation of WHIRLY1 by CIPK14 Shifts Its Localization and Dual Functions in Arabidopsis. Mol Plant, 2017. 10(5): p. 749-763 [PMID:28412544] Sarkar S, et al. Interaction of Arabidopsis TGA3 and WRKY53 transcription factors on Cestrum yellow leaf curling virus (CmYLCV) promoter mediates salicylic acid-dependent gene expression in planta. Planta, 2018. 247(1): p. 181-199 [PMID:28913593] Crespo-Salvador Ó,Escamilla-Aguilar M,López-Cruz J,López-Rodas G,González-Bosch C Determination of histone epigenetic marks in Arabidopsis and tomato genes in the early response to Botrytis cinerea. Plant Cell Rep., 2018. 37(1): p. 153-166 [PMID:29119291] Yuan N, et al. STRESS INDUCED FACTOR 2, a Leucine-Rich Repeat Kinase Regulates Basal Plant Pathogen Defense. Plant Physiol., 2018. 176(4): p. 3062-3080 [PMID:29463771]