PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Neem_944_f_2

Organism

Azadirachta indica

Taxonomic ID

124943

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Sapindales; Meliaceae; Azadirachta

Family

WRKY

Protein Properties

Length: 566aa MW: 62072.1 Da PI: 7.1261

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Neem_944_f_2	genome	NGD	View Nucleic Acid

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	76.3	3.6e-24	217	272	1	59
---SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 1 ldDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 ++Dg++WrKYGqK kg+++pr+YYrCt + +Cpv +r +ed +++++tYeg+Hnh+ Neem_944_f_2 217 MNDGCQWRKYGQKIAKGNPCPRAYYRCTVSpTCPV----QRWSEDMSILITTYEGTHNHP 272 58************************99996....56888***************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	1.0E-24	202	274	IPR003657	WRKY domain
SuperFamily	SSF118290	3.53E-22	210	274	IPR003657	WRKY domain
PROSITE profile	PS50811	23.204	212	274	IPR003657	WRKY domain
SMART	SM00774	6.4E-28	217	273	IPR003657	WRKY domain
Pfam	PF03106	1.2E-20	218	272	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 566 aa Download sequence Send to blast
MENNQDNKSL LGKACMEEKT KKSNGEEEEG RDVHAKTQKE EQLKSAKAKV GEIREENERL 60 KLLLARMVKD YQSLQNHFLN ILQQEEIKKS KGISPAHQEN EELELVSLSL GRASSEPKKL 120 DEKKNNNLIL GKEDEKCKEG LTLGLDCRFE FNSTELAEKN PSSENTFEEL KEEEPTEIWP 180 PSKILKTVRS EDEEVLQQTN LKKARVSVRA RCDTPTMNDG CQWRKYGQKI AKGNPCPRAY 240 YRCTVSPTCP VQRWSEDMSI LITTYEGTHN HPLPVSATAM ASTTSAAASM LQCRASTSQP 300 GFGTSLSASD STSTSADLHG LNFTSSENAK PHLFYFPNSS ISTSNSHPTI VLDLTAPTAF 360 SHFNRLTSAP RYNSPTCLNF SAPSSANSFE PSKTLQTSWS NGYSSYGTLN SYNKNIHVGA 420 LNLAKQQKPR EHINQPFTQM NNLSPQQSLT ETIAATTKVI TSNPSFQSAL AAAISSYIGN 480 AGSEAHQNRV GVEVSGLNLT WGESFPIKPA DSSAQNGMGC ASTFLNKLLS CNNPQQQGNL 540 SIFPPNSLPF STSKSASGSP ADGKDH

Sequence ? help Back to Top

Protein Sequence Length: 566 aa Download sequence Send to blast

MENNQDNKSL LGKACMEEKT KKSNGEEEEG RDVHAKTQKE EQLKSAKAKV GEIREENERL  60
KLLLARMVKD YQSLQNHFLN ILQQEEIKKS KGISPAHQEN EELELVSLSL GRASSEPKKL  120
DEKKNNNLIL GKEDEKCKEG LTLGLDCRFE FNSTELAEKN PSSENTFEEL KEEEPTEIWP  180
PSKILKTVRS EDEEVLQQTN LKKARVSVRA RCDTPTMNDG CQWRKYGQKI AKGNPCPRAY  240
YRCTVSPTCP VQRWSEDMSI LITTYEGTHN HPLPVSATAM ASTTSAAASM LQCRASTSQP  300
GFGTSLSASD STSTSADLHG LNFTSSENAK PHLFYFPNSS ISTSNSHPTI VLDLTAPTAF  360
SHFNRLTSAP RYNSPTCLNF SAPSSANSFE PSKTLQTSWS NGYSSYGTLN SYNKNIHVGA  420
LNLAKQQKPR EHINQPFTQM NNLSPQQSLT ETIAATTKVI TSNPSFQSAL AAAISSYIGN  480
AGSEAHQNRV GVEVSGLNLT WGESFPIKPA DSSAQNGMGC ASTFLNKLLS CNNPQQQGNL  540
SIFPPNSLPF STSKSASGSP ADGKDH

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	4e-18	204	274	1	74	WRKY transcription factor 1
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription activator involved in the transcriptional regulation of terpene biosynthesis in glandular trichomes (PubMed:24884371). Binds to the promoter of the linalool synthase TPS5 and promotes TPS5 gene transactivation (PubMed:24884371). In association with WRKY72B, contributes to basal defense against root-knot nematodes (RKNs) and potato aphids, as well as Mi-1-mediated gene-for-gene resistance to these pests (PubMed:20409007). Both WRKY72A and WRKY72B are not required for gene-for-gene resistance mediated by Pto, another tomato R gene (PubMed:20409007). {ECO:0000269\|PubMed:20409007, ECO:0000269\|PubMed:24884371}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced by infection with the root-knot nematode (RKN) Meloidogyne incognita and potato aphids. {ECO:0000269\|PubMed:20409007}.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_024042177.1	0.0	probable WRKY transcription factor 36 isoform X1
Swissprot	A0A3Q7GFB5	1e-110	WK72A_SOLLC; WRKY transcription factor 72A
TrEMBL	A0A2C9WM20	0.0	A0A2C9WM20_MANES; Uncharacterized protein
STRING	XP_006483224.1	0.0	(Citrus sinensis)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM2959	28	68

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT5G15130.1	1e-68	WRKY DNA-binding protein 72

Publications ? help Back to Top
Tomato Genome Consortium The tomato genome sequence provides insights into fleshy fruit evolution. Nature, 2012. 485(7400): p. 635-41 [PMID:22660326] Spyropoulou EA,Haring MA,Schuurink RC RNA sequencing on Solanum lycopersicum trichomes identifies transcription factors that activate terpene synthase promoters. BMC Genomics, 2014. 15: p. 402 [PMID:24884371]