PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Neem_35311_f_2

Organism

Azadirachta indica

Taxonomic ID

124943

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Sapindales; Meliaceae; Azadirachta

Family

WRKY

Protein Properties

Length: 495aa MW: 53386 Da PI: 7.9683

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Neem_35311_f_2	genome	NGD	View Nucleic Acid

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	96	2.7e-30	175	234	1	59
---SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 1 ldDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 ++Dg++WrKYGqK kg+++pr+YYrCt a gCpv+k+v+r+ ed +++++tYeg+Hnh+ Neem_35311_f_2 175 MNDGCQWRKYGQKIAKGNPCPRAYYRCTVApGCPVRKQVQRCVEDMSILITTYEGTHNHP 234 58*************************99**************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	1.0E-31	160	234	IPR003657	WRKY domain
SuperFamily	SSF118290	3.27E-27	168	235	IPR003657	WRKY domain
PROSITE profile	PS50811	27.978	170	236	IPR003657	WRKY domain
SMART	SM00774	4.5E-37	175	235	IPR003657	WRKY domain
Pfam	PF03106	4.9E-27	176	234	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 495 aa Download sequence Send to blast
MGEVREENER LKKMLEQIEK DYKSLQLKFF DILQREQPSA KKPRTDSTPP PFSQDEIMEP 60 ELVSLCLGRS SPSKKEEKTS NNSSSKTSRE SDEELKPSLS LALDSKIQPS LELAVSNLTP 120 ENSSEEPKEE EAGETWPPSK ALKTVRNNGD DEVSQQSYVK RARVSVRARC DTPTMNDGCQ 180 WRKYGQKIAK GNPCPRAYYR CTVAPGCPVR KQVQRCVEDM SILITTYEGT HNHPLSVSAT 240 AMASTTSAAA SMLLSGSSTS HSGLSSTATA TAATAQNGLN FNSLYDSSRL KPFYSPNNSS 300 PLFPTITLDL TNPSSSFSNF SRFSSGFAST PRFPSTNLSF SSSSESSMLP TLWGNGYQAF 360 GSYNQTSNGS LLNLGKQSQE HYQPFTDKNQ QAASQQALAE TLTKAITSNP SLRSVIAAAI 420 STMVGGSTNN GEQGGGGDQN LTQNGKACAS SYFNVLSSSS NSQTGSGSLL QSSLPFPILK 480 SNATPTSNVN KDQSS

Sequence ? help Back to Top

Protein Sequence Length: 495 aa Download sequence Send to blast

MGEVREENER LKKMLEQIEK DYKSLQLKFF DILQREQPSA KKPRTDSTPP PFSQDEIMEP  60
ELVSLCLGRS SPSKKEEKTS NNSSSKTSRE SDEELKPSLS LALDSKIQPS LELAVSNLTP  120
ENSSEEPKEE EAGETWPPSK ALKTVRNNGD DEVSQQSYVK RARVSVRARC DTPTMNDGCQ  180
WRKYGQKIAK GNPCPRAYYR CTVAPGCPVR KQVQRCVEDM SILITTYEGT HNHPLSVSAT  240
AMASTTSAAA SMLLSGSSTS HSGLSSTATA TAATAQNGLN FNSLYDSSRL KPFYSPNNSS  300
PLFPTITLDL TNPSSSFSNF SRFSSGFAST PRFPSTNLSF SSSSESSMLP TLWGNGYQAF  360
GSYNQTSNGS LLNLGKQSQE HYQPFTDKNQ QAASQQALAE TLTKAITSNP SLRSVIAAAI  420
STMVGGSTNN GEQGGGGDQN LTQNGKACAS SYFNVLSSSS NSQTGSGSLL QSSLPFPILK  480
SNATPTSNVN KDQSS

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	3e-20	162	235	1	73	WRKY transcription factor 1
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription activator involved in the transcriptional regulation of terpene biosynthesis in glandular trichomes (PubMed:24884371). Binds to the promoter of the linalool synthase TPS5 and promotes TPS5 gene transactivation (PubMed:24884371). In association with WRKY72B, contributes to basal defense against root-knot nematodes (RKNs) and potato aphids, as well as Mi-1-mediated gene-for-gene resistance to these pests (PubMed:20409007). Both WRKY72A and WRKY72B are not required for gene-for-gene resistance mediated by Pto, another tomato R gene (PubMed:20409007). {ECO:0000269\|PubMed:20409007, ECO:0000269\|PubMed:24884371}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced by infection with the root-knot nematode (RKN) Meloidogyne incognita and potato aphids. {ECO:0000269\|PubMed:20409007}.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_006431098.2	0.0	probable WRKY transcription factor 72
Swissprot	A0A3Q7GFB5	1e-112	WK72A_SOLLC; WRKY transcription factor 72A
TrEMBL	V4S9X4	0.0	V4S9X4_9ROSI; Uncharacterized protein
STRING	XP_006431098.1	0.0	(Citrus clementina)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM2959	28	68

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT5G15130.1	3e-86	WRKY DNA-binding protein 72

Publications ? help Back to Top
Tomato Genome Consortium The tomato genome sequence provides insights into fleshy fruit evolution. Nature, 2012. 485(7400): p. 635-41 [PMID:22660326] Spyropoulou EA,Haring MA,Schuurink RC RNA sequencing on Solanum lycopersicum trichomes identifies transcription factors that activate terpene synthase promoters. BMC Genomics, 2014. 15: p. 402 [PMID:24884371]