PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Neem_2710_f_3

Organism

Azadirachta indica

Taxonomic ID

124943

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Sapindales; Meliaceae; Azadirachta

Family

WRKY

Protein Properties

Length: 625aa MW: 67667.4 Da PI: 6.9444

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Neem_2710_f_3	genome	NGD	View Nucleic Acid

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	95.1	4.9e-30	275	334	1	59
---SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 1 ldDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 ++Dg++WrKYGqK kg+++pr+YYrCt a +Cpv+k+v+r+ +d +++++tYeg+Hnh+ Neem_2710_f_3 275 MNDGCQWRKYGQKIAKGNPCPRAYYRCTVApSCPVRKQVQRCVDDMSILITTYEGTHNHP 334 58*************************99**************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	1.6E-31	260	336	IPR003657	WRKY domain
SuperFamily	SSF118290	3.01E-27	268	336	IPR003657	WRKY domain
PROSITE profile	PS50811	28.323	270	336	IPR003657	WRKY domain
SMART	SM00774	2.3E-36	275	335	IPR003657	WRKY domain
Pfam	PF03106	1.3E-26	276	334	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 625 aa Download sequence Send to blast
MEENRAAAME DIFRRSGRRV TVDVDEEKKA ADSAADPEEI VVKIGKKRLH NENNDTIPSS 60 PEQKHLSSGK QQLDLTNAKG SSLGPNSRPS SSSQKEQDDQ LESARAEMGE VRQENQRLKM 120 YLDRIMKDYQ TLQMQFINIV QQESKKSTDR SNDHEQEIEE PEFVSLSLGR FSSDSRKDDN 180 KNKTSSQGKE DEHDKEGLSL ALDCKFEASK SDANESLPNP SPAHSSEEPK DDTGQTWPPS 240 KVLKTIRSGD DEILQQSQVK KTRVSVRARC DTPTMNDGCQ WRKYGQKIAK GNPCPRAYYR 300 CTVAPSCPVR KQVQRCVDDM SILITTYEGT HNHPLPVSAT AMASTTSAAA SMLLSGASSS 360 SLPGSSAPSA TIPGASNLHG LNFYMSDNSK SKQYYLPNSS LSTSPSHSTI TLDLTTNTPS 420 SSSSHFSTFT SNFPPAPRYS SANLNFSSSE SSSNLLWSNG LLSYSNSSTQ PYIKNQIGSL 480 NLGRQPLENV YQSFMQKNNP TLPEQSLPPD TIAAATKAIT ADPSFQSALA AALKSIIGTG 540 NSGPGGTNQV SGDNFGQKVK WGEQFPAASS HSETVKGSGC GSSYLNKSSA TNSQPGSLIF 600 LPPSLPFSAS KSASASPSDN REHTN

Sequence ? help Back to Top

Protein Sequence Length: 625 aa Download sequence Send to blast

MEENRAAAME DIFRRSGRRV TVDVDEEKKA ADSAADPEEI VVKIGKKRLH NENNDTIPSS  60
PEQKHLSSGK QQLDLTNAKG SSLGPNSRPS SSSQKEQDDQ LESARAEMGE VRQENQRLKM  120
YLDRIMKDYQ TLQMQFINIV QQESKKSTDR SNDHEQEIEE PEFVSLSLGR FSSDSRKDDN  180
KNKTSSQGKE DEHDKEGLSL ALDCKFEASK SDANESLPNP SPAHSSEEPK DDTGQTWPPS  240
KVLKTIRSGD DEILQQSQVK KTRVSVRARC DTPTMNDGCQ WRKYGQKIAK GNPCPRAYYR  300
CTVAPSCPVR KQVQRCVDDM SILITTYEGT HNHPLPVSAT AMASTTSAAA SMLLSGASSS  360
SLPGSSAPSA TIPGASNLHG LNFYMSDNSK SKQYYLPNSS LSTSPSHSTI TLDLTTNTPS  420
SSSSHFSTFT SNFPPAPRYS SANLNFSSSE SSSNLLWSNG LLSYSNSSTQ PYIKNQIGSL  480
NLGRQPLENV YQSFMQKNNP TLPEQSLPPD TIAAATKAIT ADPSFQSALA AALKSIIGTG  540
NSGPGGTNQV SGDNFGQKVK WGEQFPAASS HSETVKGSGC GSSYLNKSSA TNSQPGSLIF  600
LPPSLPFSAS KSASASPSDN REHTN

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	2e-19	262	336	1	74	WRKY transcription factor 1
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription activator involved in the transcriptional regulation of terpene biosynthesis in glandular trichomes (PubMed:24884371). Binds to the promoter of the linalool synthase TPS5 and promotes TPS5 gene transactivation (PubMed:24884371). In association with WRKY72B, contributes to basal defense against root-knot nematodes (RKNs) and potato aphids, as well as Mi-1-mediated gene-for-gene resistance to these pests (PubMed:20409007). Both WRKY72A and WRKY72B are not required for gene-for-gene resistance mediated by Pto, another tomato R gene (PubMed:20409007). {ECO:0000269\|PubMed:20409007, ECO:0000269\|PubMed:24884371}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced by infection with the root-knot nematode (RKN) Meloidogyne incognita and potato aphids. {ECO:0000269\|PubMed:20409007}.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_007037253.2	0.0	PREDICTED: probable WRKY transcription factor 61
Swissprot	A0A3Q7GFB5	1e-138	WK72A_SOLLC; WRKY transcription factor 72A
TrEMBL	A0A061G420	0.0	A0A061G420_THECC; WRKY DNA-binding protein 72, putative
STRING	EOY21754	0.0	(Theobroma cacao)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM2959	28	68

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT5G15130.1	1e-67	WRKY DNA-binding protein 72

Publications ? help Back to Top
Tomato Genome Consortium The tomato genome sequence provides insights into fleshy fruit evolution. Nature, 2012. 485(7400): p. 635-41 [PMID:22660326] Spyropoulou EA,Haring MA,Schuurink RC RNA sequencing on Solanum lycopersicum trichomes identifies transcription factors that activate terpene synthase promoters. BMC Genomics, 2014. 15: p. 402 [PMID:24884371]