PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Aqcoe5G338800.1.p

Organism

Aquilegia coerulea

Taxonomic ID

218851

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; stem eudicotyledons; Ranunculales; Ranunculaceae; Thalictroideae; Aquilegia

Family

ARF

Protein Properties

Length: 846aa MW: 94200.4 Da PI: 6.4507

Description

ARF family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Aqcoe5G338800.1.p	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	B3	71.8	8.5e-23	148	249	1	99
EEEE-..-HHHHTT-EE--HHH.HTT.......---..--SEEEEEETTS-EEEEEE..EEETTEEEE-TTHHHHHHHHT--TT-EEEEEE- CS B3 1 ffkvltpsdvlksgrlvlpkkfaeeh.......ggkkeesktltledesgrsWevkliyrkksgryvltkGWkeFvkangLkegDfvvFkld 85 f+k+lt sd++++g +++ +++a+e+ +++++ +l+ +d++g +W++++i+r++++r++l++GW+ Fv++++L +gD ++F Aqcoe5G338800.1.p 148 FCKTLTASDTSTHGGFSVLRRHADEClppldmcRQPPTQ--ELVAKDLHGIEWKFRHIFRGQPRRHLLQSGWSVFVSSKRLVAGDAFIFL-- 235 99***************************87555544..8********************************************.. PP SSSEE..EEEEE-S CS B3 86 grsefelvvkvfrk 99 + +++el+v+v+r+ Aqcoe5G338800.1.p 236 RGENGELRVGVRRA 249 449999***985 PP
2	Auxin_resp	119.3	3e-39	274	356	1	83
Auxin_resp 1 aahaastksvFevvYnPrastseFvvkvekvekalkvkvsvGmRfkmafetedsserrlsGtvvgvsdldpvrWpnSkWrsLk 83 a+ha+st+++F+v+Y+Pr+s++eF+v+++++++++k+ +s+GmRfkm+fe+e+++e+r++Gt+vg+ d+d+ rW++SkWr+Lk Aqcoe5G338800.1.p 274 AWHAVSTGTMFTVYYKPRTSPAEFIVPYDQYMESVKNTYSIGMRFKMRFEGEEAPEQRFTGTIVGMGDADSNRWHDSKWRCLK 356 89*******************************************************************************96 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF101936	5.89E-40	141	277	IPR015300	DNA-binding pseudobarrel domain
Gene3D	G3DSA:2.40.330.10	3.0E-40	141	263	IPR015300	DNA-binding pseudobarrel domain
CDD	cd10017	3.41E-21	146	248	No hit	No description
PROSITE profile	PS50863	11.462	148	250	IPR003340	B3 DNA binding domain
Pfam	PF02362	1.4E-20	148	249	IPR003340	B3 DNA binding domain
SMART	SM01019	5.7E-22	148	250	IPR003340	B3 DNA binding domain
Pfam	PF06507	9.4E-37	274	356	IPR010525	Auxin response factor
Pfam	PF02309	2.7E-12	704	810	IPR033389	AUX/IAA domain
PROSITE profile	PS51745	26.02	721	805	IPR000270	PB1 domain
SuperFamily	SSF54277	7.85E-13	722	798	No hit	No description

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0008285	Biological Process	negative regulation of cell proliferation
GO:0009737	Biological Process	response to abscisic acid
GO:0009911	Biological Process	positive regulation of flower development
GO:0010047	Biological Process	fruit dehiscence
GO:0010150	Biological Process	leaf senescence
GO:0010227	Biological Process	floral organ abscission
GO:0045892	Biological Process	negative regulation of transcription, DNA-templated
GO:0048481	Biological Process	plant ovule development
GO:0005634	Cellular Component	nucleus
GO:0005515	Molecular Function	protein binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 846 aa Download sequence Send to blast
MATSEISSKN GRGESCYSSG NDGGGKAMNG GFSNYKGSEE ALYTELWHAC AGPLVTVPRE 60 GERVFYFPQG HIEQVEASTN QVADKQMPVY NLRSKILCRV INVELKAEPD TDEVFAQVTL 120 LPEDNQDENA VEKEPSPPPP PRPHVHSFCK TLTASDTSTH GGFSVLRRHA DECLPPLDMC 180 RQPPTQELVA KDLHGIEWKF RHIFRGQPRR HLLQSGWSVF VSSKRLVAGD AFIFLRGENG 240 ELRVGVRRAL RQQSNVPSSV ISSHSMHLGV LATAWHAVST GTMFTVYYKP RTSPAEFIVP 300 YDQYMESVKN TYSIGMRFKM RFEGEEAPEQ RFTGTIVGMG DADSNRWHDS KWRCLKVRWD 360 ETSSVPRPER VSPWKIEPAM APALNTLPIS RPKRPRAVAA SPDSSVLTKE GSSKITIDSA 420 SVNGFSRVLQ GQETSTLKNS LSENNESESV QRPVTWPPSQ DDGKIDMVCG QNRYGTENWA 480 PMVRHEPTYT DLLSGFRSSN DSPHALATSF SERNIEDTNP LKKQWQDSEG KLVALPWSMM 540 PPSQSMNTLD SDMKIHSQAN EISYPKAGTK YNAFSGYPVL QNIRVEQHEG NWMMPLLSSP 600 HSETLHHPRG IRPQPSYFQQ HEVSKSKTNG NCKLFGIPLN VNPVVPEPAA PHGNVPYEPE 660 NHITSALPPQ KALDSDHQSE QSKASKSTDT AVLGNEQEKS TPGCQQQRRD VKSKLQGGST 720 RSCTKVHKQG IALGRSVDLT KFSGYDELLT ELDQMFDFDG ELMAHSKIWQ VVYTDNEGDM 780 MLVGDDPWQE FCSMVRKIFI YTREEVQKMT PGALNARAED NPTNAEERTG EQETSCAAAA 840 SPENC*

Sequence ? help Back to Top

Protein Sequence Length: 846 aa Download sequence Send to blast

MATSEISSKN GRGESCYSSG NDGGGKAMNG GFSNYKGSEE ALYTELWHAC AGPLVTVPRE  60
GERVFYFPQG HIEQVEASTN QVADKQMPVY NLRSKILCRV INVELKAEPD TDEVFAQVTL  120
LPEDNQDENA VEKEPSPPPP PRPHVHSFCK TLTASDTSTH GGFSVLRRHA DECLPPLDMC  180
RQPPTQELVA KDLHGIEWKF RHIFRGQPRR HLLQSGWSVF VSSKRLVAGD AFIFLRGENG  240
ELRVGVRRAL RQQSNVPSSV ISSHSMHLGV LATAWHAVST GTMFTVYYKP RTSPAEFIVP  300
YDQYMESVKN TYSIGMRFKM RFEGEEAPEQ RFTGTIVGMG DADSNRWHDS KWRCLKVRWD  360
ETSSVPRPER VSPWKIEPAM APALNTLPIS RPKRPRAVAA SPDSSVLTKE GSSKITIDSA  420
SVNGFSRVLQ GQETSTLKNS LSENNESESV QRPVTWPPSQ DDGKIDMVCG QNRYGTENWA  480
PMVRHEPTYT DLLSGFRSSN DSPHALATSF SERNIEDTNP LKKQWQDSEG KLVALPWSMM  540
PPSQSMNTLD SDMKIHSQAN EISYPKAGTK YNAFSGYPVL QNIRVEQHEG NWMMPLLSSP  600
HSETLHHPRG IRPQPSYFQQ HEVSKSKTNG NCKLFGIPLN VNPVVPEPAA PHGNVPYEPE  660
NHITSALPPQ KALDSDHQSE QSKASKSTDT AVLGNEQEKS TPGCQQQRRD VKSKLQGGST  720
RSCTKVHKQG IALGRSVDLT KFSGYDELLT ELDQMFDFDG ELMAHSKIWQ VVYTDNEGDM  780
MLVGDDPWQE FCSMVRKIFI YTREEVQKMT PGALNARAED NPTNAEERTG EQETSCAAAA  840
SPENC*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
4ldv_A	1e-179	15	378	4	354	Auxin response factor 1
4ldw_A	1e-179	15	378	4	354	Auxin response factor 1
4ldw_B	1e-179	15	378	4	354	Auxin response factor 1
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Functional Description ? help Back to Top
Source	Description
UniProt	Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs) (By similarity). Could act as transcriptional activator or repressor (PubMed:26959229, PubMed:26716451, PubMed:27645097, PubMed:28167023). Involved in the control of fruit ripening process (PubMed:26959229, PubMed:26716451, PubMed:28167023). Regulates expression of a number of ripening regulators, transcription factors, and ethylene biosynthesis and signaling components (PubMed:26959229, PubMed:26716451). May act as a transcriptional repressor of auxin-responsive genes (PubMed:26716451). Regulates vegetative growth, lateral root formation and flower organ senescence, possibly partially by regulating gene expression of auxin and ethylene response factor (ERF) genes (PubMed:28167023). Plays a negative role in axillary shoot meristem formation (PubMed:27645097). {ECO:0000255\|RuleBase:RU004561, ECO:0000269\|PubMed:26716451, ECO:0000269\|PubMed:26959229, ECO:0000269\|PubMed:27645097, ECO:0000269\|PubMed:28167023}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00574	DAP	Transfer from AT5G62000	Download

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By tomato fruit ripening. Expression in tomato fruits is up-regulated by exogenous application of ethylene and down-regulated by inhibition of ethylene receptors with 1-methylcyclopropene (1-MCP) (PubMed:26959229, PubMed:26716451). Up-regulated by plant hormone abscisic acid (ABA) in tomato fruits (PubMed:26959229). Expression is not up-regulated by plant hormone auxin in tomato fruits (PubMed:26716451). Up-regulated by auxin and gibberellic acid (GA), and down-regulated by ethylene in seedlings (PubMed:28167023). Down-regulated during decapitation-induced axillary shoot development and by excision of immature leaves. Down-regulation is inhibited by the application of auxin on the cut surface (PubMed:27645097). {ECO:0000269\|PubMed:26716451, ECO:0000269\|PubMed:26959229, ECO:0000269\|PubMed:27645097, ECO:0000269\|PubMed:28167023}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_010249209.1	0.0	PREDICTED: auxin response factor 2-like
Swissprot	Q2LAJ3	0.0	ARF2A_SOLLC; Auxin response factor 2A
TrEMBL	A0A2G5C474	0.0	A0A2G5C474_AQUCA; Auxin response factor
STRING	Aquca_100_00036.1	0.0	(Aquilegia coerulea)

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT5G62000.3	0.0	auxin response factor 2

Link Out ? help Back to Top
Phytozome	Aqcoe5G338800.1.p

Publications ? help Back to Top
Tomato Genome Consortium The tomato genome sequence provides insights into fleshy fruit evolution. Nature, 2012. 485(7400): p. 635-41 [PMID:22660326] Breitel DA, et al. AUXIN RESPONSE FACTOR 2 Intersects Hormonal Signals in the Regulation of Tomato Fruit Ripening. PLoS Genet., 2016. 12(3): p. e1005903 [PMID:26959229] Xu T, et al. SlARF2a plays a negative role in mediating axillary shoot formation. Sci Rep, 2016. 6: p. 33728 [PMID:27645097] Ren Z,Liu R,Gu W,Dong X The Solanum lycopersicum auxin response factor SlARF2 participates in regulating lateral root formation and flower organ senescence. Plant Sci., 2017. 256: p. 103-111 [PMID:28167023]