PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Convolvulaceae; Ipomoeeae; Ipomoea

Family

WRKY

Protein Properties

Length: 549aa MW: 59135 Da PI: 7.312

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Itr_sc000017.1_g00035.1	genome	Kazusa	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	96	2.5e-30	294	352	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 +Dg++WrKYGqK+ kg+++pr+YYrCt+a Cpv+k+v+r+aed +++++tYeg+Hnh+ Itr_sc000017.1_g00035.1 294 SDGCQWRKYGQKMAKGNPCPRAYYRCTMAvACPVRKQVQRCAEDRTILTTTYEGTHNHP 352 7**************************9**************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	1.8E-32	278	354	IPR003657	WRKY domain
SuperFamily	SSF118290	4.84E-27	286	354	IPR003657	WRKY domain
PROSITE profile	PS50811	28.174	288	354	IPR003657	WRKY domain
SMART	SM00774	6.5E-35	293	353	IPR003657	WRKY domain
Pfam	PF03106	7.3E-26	295	352	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 549 aa Download sequence Send to blast
MENGWGLTLE NSIFKNGLGS SRPRFDTANM FPVKKDDGER RQQGVLNEVD FFSEKKKPVD 60 SGFVVKKETS NDEPIRTDLN INTGLQLVTA NTGSDQSTID DGVSSGMEER RVKDEVAGLQ 120 AELERMNGEN QRLKGMLTQV TNNYTALEMH LAALMQQQKK NSMAESVHEV VDRKSEEKKY 180 EKEGSTVPRQ FLDLGPSGLT GAEQTDEPTH SPTTSSEEKT ISASPRNNVD SSKHKRSARE 240 ESPDLESWNP NKAPKSIISS SSSRPVDDQQ ASTDATMRKA RVSVRARSEA PMISDGCQWR 300 KYGQKMAKGN PCPRAYYRCT MAVACPVRKQ VQRCAEDRTI LTTTYEGTHN HPLPPAAMAM 360 ASTTSAAANM LLSGSMPSAD MMMNPNFLAR AILPCSSSVA TISASAPFPT VTLDLTQTPN 420 SLPNYPRLPP SQFPGAGAGA PHSLPGFAVT PPQVFGQGLY SQSKFSGLQV SHEAQHPLLP 480 PAHPSLSDTL SAATAAITAD PNFTAALAAA ISSILNGSQP NIVNSTNNNT TATANAPNSN 540 PTNNCNKTH

Sequence ? help Back to Top

Protein Sequence Length: 549 aa Download sequence Send to blast

MENGWGLTLE NSIFKNGLGS SRPRFDTANM FPVKKDDGER RQQGVLNEVD FFSEKKKPVD  60
SGFVVKKETS NDEPIRTDLN INTGLQLVTA NTGSDQSTID DGVSSGMEER RVKDEVAGLQ  120
AELERMNGEN QRLKGMLTQV TNNYTALEMH LAALMQQQKK NSMAESVHEV VDRKSEEKKY  180
EKEGSTVPRQ FLDLGPSGLT GAEQTDEPTH SPTTSSEEKT ISASPRNNVD SSKHKRSARE  240
ESPDLESWNP NKAPKSIISS SSSRPVDDQQ ASTDATMRKA RVSVRARSEA PMISDGCQWR  300
KYGQKMAKGN PCPRAYYRCT MAVACPVRKQ VQRCAEDRTI LTTTYEGTHN HPLPPAAMAM  360
ASTTSAAANM LLSGSMPSAD MMMNPNFLAR AILPCSSSVA TISASAPFPT VTLDLTQTPN  420
SLPNYPRLPP SQFPGAGAGA PHSLPGFAVT PPQVFGQGLY SQSKFSGLQV SHEAQHPLLP  480
PAHPSLSDTL SAATAAITAD PNFTAALAAA ISSILNGSQP NIVNSTNNNT TATANAPNSN  540
PTNNCNKTH

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	3e-20	280	356	1	76	WRKY transcription factor 1
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor involved in the control of processes related to senescence and pathogen defense (PubMed:12000796). Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (PubMed:12000796). Activates the transcription of the SIRK gene and represses its own expression and that of the WRKY42 genes (PubMed:12000796). Modulates phosphate homeostasis and Pi translocation by regulating PHO1 expression (PubMed:25733771). {ECO:0000269\|PubMed:12000796, ECO:0000269\|PubMed:25733771}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By salicylic acid, ethylene, jasmonic acid, pathogens, wounding and strongly during leaf senescence. {ECO:0000269\|PubMed:11449049, ECO:0000269\|PubMed:11722756}.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_019171769.1	0.0	PREDICTED: probable WRKY transcription factor 31
Swissprot	Q9C519	1e-144	WRKY6_ARATH; WRKY transcription factor 6
TrEMBL	A0A1S3Z416	0.0	A0A1S3Z416_TOBAC; probable WRKY transcription factor 31 isoform X2
TrEMBL	A0A1U7WGI9	0.0	A0A1U7WGI9_NICSY; probable WRKY transcription factor 31 isoform X2
STRING	XP_009776375.1	0.0	(Nicotiana sylvestris)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Asterids	OGEA945	24	92

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT1G62300.1	1e-118	WRKY family protein

Publications ? help Back to Top
Zhou F, et al. High humidity suppresses ssi4-mediated cell death and disease resistance upstream of MAP kinase activation, H2O2 production and defense gene expression. Plant J., 2004. 39(6): p. 920-32 [PMID:15341634] Ramírez V,López A,Mauch-Mani B,Gil MJ,Vera P An extracellular subtilase switch for immune priming in Arabidopsis. PLoS Pathog., 2013. 9(6): p. e1003445 [PMID:23818851] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Singh V,Roy S,Singh D,Nandi AK Arabidopsis flowering locus D influences systemic-acquired-resistance- induced expression and histone modifications of WRKY genes. J. Biosci., 2014. 39(1): p. 119-26 [PMID:24499796] Ali MA,Wieczorek K,Kreil DP,Bohlmann H The beet cyst nematode Heterodera schachtii modulates the expression of WRKY transcription factors in syncytia to favour its development in Arabidopsis roots. PLoS ONE, 2014. 9(7): p. e102360 [PMID:25033038] Chai J,Liu J,Zhou J,Xing D Mitogen-activated protein kinase 6 regulates NPR1 gene expression and activation during leaf senescence induced by salicylic acid. J. Exp. Bot., 2014. 65(22): p. 6513-28 [PMID:25210078] Vermeirssen V,De Clercq I,Van Parys T,Van Breusegem F,Van de Peer Y Arabidopsis ensemble reverse-engineered gene regulatory network discloses interconnected transcription factors in oxidative stress. Plant Cell, 2014. 26(12): p. 4656-79 [PMID:25549671] Xiao D, et al. SENESCENCE-SUPPRESSED PROTEIN PHOSPHATASE Directly Interacts with the Cytoplasmic Domain of SENESCENCE-ASSOCIATED RECEPTOR-LIKE KINASE and Negatively Regulates Leaf Senescence in Arabidopsis. Plant Physiol., 2015. 169(2): p. 1275-91 [PMID:26304848] Bakshi M, et al. WRKY6 restricts Piriformospora indica-stimulated and phosphate-induced root development in Arabidopsis. BMC Plant Biol., 2015. 15: p. 305 [PMID:26718529] Huang Y,Feng CZ,Ye Q,Wu WH,Chen YF Arabidopsis WRKY6 Transcription Factor Acts as a Positive Regulator of Abscisic Acid Signaling during Seed Germination and Early Seedling Development. PLoS Genet., 2016. 12(2): p. e1005833 [PMID:26829043] Ramírez V,López A,Mauch-Mani B,Gil MJ,Vera P Correction: An Extracellular Subtilase Switch for Immune Priming in Arabidopsis. PLoS Pathog., 2016. 12(11): p. e1006003 [PMID:27806116] Li H, et al. BZR1 Positively Regulates Freezing Tolerance via CBF-Dependent and CBF-Independent Pathways in Arabidopsis. Mol Plant, 2017. 10(4): p. 545-559 [PMID:28089951] Stetter MG,Benz M,Ludewig U Increased root hair density by loss of WRKY6 in Arabidopsis thaliana. PeerJ, 2017. 5: p. e2891 [PMID:28149680] Zhou X, et al. StMYB44 negatively regulates phosphate transport by suppressing expression of PHOSPHATE1 in potato. J. Exp. Bot., 2017. 68(5): p. 1265-1281 [PMID:28338870] Bakshi M, et al. Piriformospora indica Reprograms Gene Expression in Arabidopsis Phosphate Metabolism Mutants But Does Not Compensate for Phosphate Limitation. Front Microbiol, 2017. 8: p. 1262 [PMID:28747898] Zhang Y, et al. DELLA proteins negatively regulate dark-induced senescence and chlorophyll degradation in Arabidopsis through interaction with the transcription factor WRKY6. Plant Cell Rep., 2018. 37(7): p. 981-992 [PMID:29574486]