PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID Cucsa.282940.1
Common NameCsa_5G612310, LOC101206349
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis
Family AP2
Protein Properties Length: 440aa    MW: 49196.4 Da    PI: 5.9839
Description AP2 family protein
Gene Model
Gene Model ID Type Source Coding Sequence
Cucsa.282940.1genomeJGIView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1AP249.21.3e-1583142155
             AP2   1 sgykGVrwdkkrgrWvAeIrd.pseng...kr.krfslgkfgtaeeAakaaiaarkkleg 55 
                     s y+GV++++++gr++A+++d  s+n+   k+ ++++lg ++ +e Aa+ ++ a++k++g
  Cucsa.282940.1  83 SIYRGVTRHRWTGRFEAHLWDkSSWNNiqnKKgRQVYLGAYDNEEAAARTYDLAALKYWG 142
                     57*******************777799977447*************************98 PP

2AP252.21.6e-16185237156
             AP2   1 sgykGVrwdkkrgrWvAeIrd.psengkrkrfslgkfgtaeeAakaaiaarkklege 56 
                     s+y+GV +++ +grW+A+I   +   g +k+ +lg++ t eeAa+a++ a+ +++g+
  Cucsa.282940.1 185 SKYRGVARHHHNGRWEARIGRvF---G-SKYLYLGTYNTQEEAAAAYDMAAIEYRGA 237
                     89****99**********99966...3.5*************************995 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS512575117No hitNo description
PfamPF008474.5E-1283142IPR001471AP2/ERF domain
SuperFamilySSF541714.84E-1683152IPR016177DNA-binding domain
PROSITE profilePS5103219.87584150IPR001471AP2/ERF domain
SMARTSM003806.1E-2484156IPR001471AP2/ERF domain
Gene3DG3DSA:3.30.730.105.6E-1584151IPR001471AP2/ERF domain
CDDcd000182.41E-1984151No hitNo description
PRINTSPR003672.8E-68596IPR001471AP2/ERF domain
PfamPF008474.0E-12185236IPR001471AP2/ERF domain
SuperFamilySSF541717.85E-18185246IPR016177DNA-binding domain
CDDcd000181.59E-15185246No hitNo description
SMARTSM003806.0E-24186250IPR001471AP2/ERF domain
PROSITE profilePS5103218.294186244IPR001471AP2/ERF domain
Gene3DG3DSA:3.30.730.103.6E-18186244IPR001471AP2/ERF domain
PRINTSPR003672.8E-6226246IPR001471AP2/ERF domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006110Biological Processregulation of glycolytic process
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009744Biological Processresponse to sucrose
GO:0019432Biological Processtriglyceride biosynthetic process
GO:1901959Biological Processpositive regulation of cutin biosynthetic process
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
Sequence ? help Back to Top
Protein Sequence    Length: 440 aa     Download sequence    Send to blast
MRISSSSSSS SSSSSSCVGS DSGIHNNNNL TPAPQSEKTR AKRRRRNQDE TKCQIHNHNA  60
NETNNPAAAA SASASASSAR RSSIYRGVTR HRWTGRFEAH LWDKSSWNNI QNKKGRQVYL  120
GAYDNEEAAA RTYDLAALKY WGPGTTLNFP VESYTNEMEA MRKVTKEEYL ASLRRRSSGF  180
SRGVSKYRGV ARHHHNGRWE ARIGRVFGSK YLYLGTYNTQ EEAAAAYDMA AIEYRGANAV  240
TNFDISNYIG RLENKSSLLQ EEATQQTDDP NYSPVSSEGE VVQQQQQDQQ QQQQQQQTTF  300
SSPPDLHFSI ENNPAMVIMD EPPTQDDHDL HWSFLDTGLF VQVPDLPLEK SSELADLYFD  360
EIGFEDDIGM MFEASLENNN CEANNNNNNS SNSNKNNNNN NNNVGKMEVN CSEKIRLFST  420
TTSPSSSSIT TSVSCEFRV*
Functional Description ? help Back to Top
Source Description
UniProtMay be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). Transcriptional activator involved in the activation of a subset of sugar-responsive genes and the control of carbon flow from sucrose import to oil accumulation in developing seeds. Binds to the GCC-box pathogenesis-related promoter element. Promotes sugar uptake and seed oil accumulation by glycolysis. Required for embryo development, seed germination and, indirectly, for seedling establishment. Negative regulator of the ABA-mediated germination inhibition. {ECO:0000250, ECO:0000269|PubMed:12084821, ECO:0000269|PubMed:15500472, ECO:0000269|PubMed:15753106, ECO:0000269|PubMed:16553903, ECO:0000269|PubMed:16632590, ECO:0000269|PubMed:9733529}.
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Transiantly in leaves by sucrose, but not by abscisic acid (ABA). {ECO:0000269|PubMed:15753106}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankLN6818960.0LN681896.1 Cucumis melo genomic scaffold, anchoredscaffold00016.
GenBankLN7132640.0LN713264.1 Cucumis melo genomic chromosome, chr_10.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_011655821.10.0PREDICTED: ethylene-responsive transcription factor WRI1
SwissprotQ6X5Y61e-114WRI1_ARATH; Ethylene-responsive transcription factor WRI1
TrEMBLA0A0A0KWK10.0A0A0A0KWK1_CUCSA; Uncharacterized protein
STRINGXP_008446712.10.0(Cucumis melo)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
FabidsOGEF98593241
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT3G54320.35e-94AP2 family protein
Publications ? help Back to Top
  1. Ren Y, et al.
    An integrated genetic and cytogenetic map of the cucumber genome.
    PLoS ONE, 2009. 4(6): p. e5795
    [PMID:19495411]
  2. Guo S, et al.
    Transcriptome sequencing and comparative analysis of cucumber flowers with different sex types.
    BMC Genomics, 2010. 11: p. 384
    [PMID:20565788]
  3. Li Z, et al.
    RNA-Seq improves annotation of protein-coding genes in the cucumber genome.
    BMC Genomics, 2011. 12: p. 540
    [PMID:22047402]
  4. Kim HU, et al.
    Ectopic overexpression of castor bean LEAFY COTYLEDON2 (LEC2) in Arabidopsis triggers the expression of genes that encode regulators of seed maturation and oil body proteins in vegetative tissues.
    FEBS Open Bio, 2013. 4: p. 25-32
    [PMID:24363987]
  5. Wu XL,Liu ZH,Hu ZH,Huang RZ
    BnWRI1 coordinates fatty acid biosynthesis and photosynthesis pathways during oil accumulation in rapeseed.
    J Integr Plant Biol, 2014. 56(6): p. 582-93
    [PMID:24393360]
  6. Shigematsu H, et al.
    Structural characterization of the mechanosensitive channel candidate MCA2 from Arabidopsis thaliana.
    PLoS ONE, 2014. 9(1): p. e87724
    [PMID:24475319]
  7. Kim HU, et al.
    Senescence-inducible LEC2 enhances triacylglycerol accumulation in leaves without negatively affecting plant growth.
    Plant Biotechnol. J., 2015. 13(9): p. 1346-59
    [PMID:25790072]
  8. Grimberg Å,Carlsson AS,Marttila S,Bhalerao R,Hofvander P
    Transcriptional transitions in Nicotiana benthamiana leaves upon induction of oil synthesis by WRINKLED1 homologs from diverse species and tissues.
    BMC Plant Biol., 2015. 15: p. 192
    [PMID:26253704]
  9. Kanai M,Mano S,Kondo M,Hayashi M,Nishimura M
    Extension of oil biosynthesis during the mid-phase of seed development enhances oil content in Arabidopsis seeds.
    Plant Biotechnol. J., 2016. 14(5): p. 1241-50
    [PMID:26503031]
  10. Li Q, et al.
    Wrinkled1 Accelerates Flowering and Regulates Lipid Homeostasis between Oil Accumulation and Membrane Lipid Anabolism in Brassica napus.
    Front Plant Sci, 2015. 6: p. 1015
    [PMID:26635841]
  11. Hofvander P, et al.
    Potato tuber expression of Arabidopsis WRINKLED1 increase triacylglycerol and membrane lipids while affecting central carbohydrate metabolism.
    Plant Biotechnol. J., 2016. 14(9): p. 1883-98
    [PMID:26914183]
  12. Shen SL, et al.
    CitAP2.10 activation of the terpene synthase CsTPS1 is associated with the synthesis of (+)-valencene in 'Newhall' orange.
    J. Exp. Bot., 2016. 67(14): p. 4105-15
    [PMID:27194737]
  13. Adhikari ND,Bates PD,Browse J
    WRINKLED1 Rescues Feedback Inhibition of Fatty Acid Synthesis in Hydroxylase-Expressing Seeds.
    Plant Physiol., 2016. 171(1): p. 179-91
    [PMID:27208047]
  14. Kim MJ,Jang IC,Chua NH
    The Mediator Complex MED15 Subunit Mediates Activation of Downstream Lipid-Related Genes by the WRINKLED1 Transcription Factor.
    Plant Physiol., 2016. 171(3): p. 1951-64
    [PMID:27246098]
  15. Bhattacharya S,Das N,Maiti MK
    Cumulative effect of heterologous AtWRI1 gene expression and endogenous BjAGPase gene silencing increases seed lipid content in Indian mustard Brassica juncea.
    Plant Physiol. Biochem., 2016. 107: p. 204-213
    [PMID:27314514]
  16. Ma W, et al.
    14-3-3 protein mediates plant seed oil biosynthesis through interaction with AtWRI1.
    Plant J., 2016. 88(2): p. 228-235
    [PMID:27322486]
  17. Li D, et al.
    MYB89 Transcription Factor Represses Seed Oil Accumulation.
    Plant Physiol., 2017. 173(2): p. 1211-1225
    [PMID:27932421]
  18. Ivarson E, et al.
    Effects of Overexpression of WRI1 and Hemoglobin Genes on the Seed Oil Content of Lepidium campestre.
    Front Plant Sci, 2016. 7: p. 2032
    [PMID:28119714]
  19. An D, et al.
    Expression of Camelina WRINKLED1 Isoforms Rescue the Seed Phenotype of the Arabidopsis wri1 Mutant and Increase the Triacylglycerol Content in Tobacco Leaves.
    Front Plant Sci, 2017. 8: p. 34
    [PMID:28174580]
  20. Zhai Z,Liu H,Shanklin J
    Phosphorylation of WRINKLED1 by KIN10 Results in Its Proteasomal Degradation, Providing a Link between Energy Homeostasis and Lipid Biosynthesis.
    Plant Cell, 2017. 29(4): p. 871-889
    [PMID:28314829]
  21. Jin J, et al.
    Transcriptome and functional analysis reveals hybrid vigor for oil biosynthesis in oil palm.
    Sci Rep, 2017. 7(1): p. 439
    [PMID:28348403]
  22. Kong Q, et al.
    The Arabidopsis WRINKLED1 transcription factor affects auxin homeostasis in roots.
    J. Exp. Bot., 2017. 68(16): p. 4627-4634
    [PMID:28981783]
  23. Kang NK, et al.
    Increased lipid production by heterologous expression of AtWRI1 transcription factor in Nannochloropsis salina.
    Biotechnol Biofuels, 2017. 10: p. 231
    [PMID:29046718]
  24. Pellaud S, et al.
    WRINKLED1 and ACYL-COA:DIACYLGLYCEROL ACYLTRANSFERASE1 regulate tocochromanol metabolism in Arabidopsis.
    New Phytol., 2018. 217(1): p. 245-260
    [PMID:29105089]
  25. Hanano A,Almousally I,Shaban M,Murphy DJ
    Arabidopsis plants exposed to dioxin result in a WRINKLED seed phenotype due to 20S proteasomal degradation of WRI1.
    J. Exp. Bot., 2018. 69(7): p. 1781-1794
    [PMID:29394403]