PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis

Family

WRKY

Protein Properties

Length: 507aa MW: 54874.6 Da PI: 6.1728

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Cucsa.093390.2	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	78.8	6e-25	193	249	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 +DgynWrKYGqK+vk+ + +rsYY+Ct+++C +kk +e++++ ++++ei+Y+++H h+ Cucsa.093390.2 193 SDGYNWRKYGQKQVKSPKGSRSYYKCTYSECFAKK-IECCDDSGQTTEIVYKSQHSHD 249 7******************************98.*******************7 PP
2	WRKY	98.3	5.1e-31	368	424	3	59
-SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 3 DgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 Dgy+WrKYGqK+vkg+++pr+YYrCtsagCpv+k++e + e+p++v+itY+g H+h+ Cucsa.093390.2 368 DGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENPNAVIITYKGVHDHD 424 9*******************************************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50811	17.488	187	251	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	5.1E-21	187	251	IPR003657	WRKY domain
SuperFamily	SSF118290	2.88E-20	190	251	IPR003657	WRKY domain
SMART	SM00774	7.8E-24	192	250	IPR003657	WRKY domain
Pfam	PF03106	1.2E-17	194	249	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	5.1E-32	351	426	IPR003657	WRKY domain
PROSITE profile	PS50811	32.179	361	426	IPR003657	WRKY domain
SuperFamily	SSF118290	3.53E-27	362	426	IPR003657	WRKY domain
SMART	SM00774	3.0E-35	366	425	IPR003657	WRKY domain
Pfam	PF03106	7.0E-24	368	423	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 507 aa Download sequence Send to blast
MAEPESFGTH QLGSSNAATE GQEDDEEEMD DSDDESEVDL GGEGGGTGSE FKPTELTTGS 60 SLNEALVTGS LSETLTVASS AENGPSDGLQ PDEGAELKQA PSSHSEPLAV EATQTDKVQE 120 QNRLQLTIFK GPDSDQSPTS VTQSISSYAS SNLSEHKLSP KRVQKICKPE PSQKNFFNHK 180 TPSSVPNART PASDGYNWRK YGQKQVKSPK GSRSYYKCTY SECFAKKIEC CDDSGQTTEI 240 VYKSQHSHDP PRKISTPKES KLVPYVEPVV KKIIAEHSRR VINDSDSPTP SKEPLREAAI 300 VVFERKRQHS NDSNGNDEYK IKDENDDEPG TKQIVKKSSA GNSGTPLKPG KKPKFVVHAA 360 GDVGISGDGY RWRKYGQKMV KGNPHPRNYY RCTSAGCPVR KHIESAVENP NAVIITYKGV 420 HDHDTPVPKK RHGPPSALLV AAAAPASMSS NTQPKKTDVV ESQISSTQWS VDAEGELTGE 480 ALELGGEKAM ESARTLLSIG FEIKPC*

Sequence ? help Back to Top

Protein Sequence Length: 507 aa Download sequence Send to blast

MAEPESFGTH QLGSSNAATE GQEDDEEEMD DSDDESEVDL GGEGGGTGSE FKPTELTTGS  60
SLNEALVTGS LSETLTVASS AENGPSDGLQ PDEGAELKQA PSSHSEPLAV EATQTDKVQE  120
QNRLQLTIFK GPDSDQSPTS VTQSISSYAS SNLSEHKLSP KRVQKICKPE PSQKNFFNHK  180
TPSSVPNART PASDGYNWRK YGQKQVKSPK GSRSYYKCTY SECFAKKIEC CDDSGQTTEI  240
VYKSQHSHDP PRKISTPKES KLVPYVEPVV KKIIAEHSRR VINDSDSPTP SKEPLREAAI  300
VVFERKRQHS NDSNGNDEYK IKDENDDEPG TKQIVKKSSA GNSGTPLKPG KKPKFVVHAA  360
GDVGISGDGY RWRKYGQKMV KGNPHPRNYY RCTSAGCPVR KHIESAVENP NAVIITYKGV  420
HDHDTPVPKK RHGPPSALLV AAAAPASMSS NTQPKKTDVV ESQISSTQWS VDAEGELTGE  480
ALELGGEKAM ESARTLLSIG FEIKPC*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wj2_A	3e-25	357	426	8	77	Probable WRKY transcription factor 4
2lex_A	3e-25	357	426	8	77	Probable WRKY transcription factor 4
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Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. {ECO:0000250\|UniProtKB:Q9SI37}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	GU984009	0.0	GU984009.1 Cucumis sativus WRKY protein (WRKY4) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_001267648.1	0.0	probable WRKY transcription factor 32-like
Swissprot	P59583	1e-125	WRK32_ARATH; Probable WRKY transcription factor 32
TrEMBL	E7CEV8	0.0	E7CEV8_CUCSA; WRKY protein
STRING	XP_004161869.1	0.0	(Cucumis sativus)

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G30935.1	1e-104	WRKY DNA-binding protein 32

Link Out ? help Back to Top
Phytozome	Cucsa.093390.2
Entrez Gene	101209954

Publications ? help Back to Top
Ren Y, et al. An integrated genetic and cytogenetic map of the cucumber genome. PLoS ONE, 2009. 4(6): p. e5795 [PMID:19495411] Guo S, et al. Transcriptome sequencing and comparative analysis of cucumber flowers with different sex types. BMC Genomics, 2010. 11: p. 384 [PMID:20565788] Ling J, et al. Genome-wide analysis of WRKY gene family in Cucumis sativus. BMC Genomics, 2011. 12: p. 471 [PMID:21955985] Li Z, et al. RNA-Seq improves annotation of protein-coding genes in the cucumber genome. BMC Genomics, 2011. 12: p. 540 [PMID:22047402]