PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT5G45260.1

Common Name

ATWRKY52, RCH2, RRS1, RRS1-S, RSH4, SLH1, WRKY52

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

WRKY

Protein Properties

Length: 1288aa MW: 145879 Da PI: 6.3536

Description

Disease resistance protein (TIR-NBS-LRR class)

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT5G45260.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	83	2.9e-26	1209	1268	3	59
-SS-EEEEEEE--TT-SS-EEEEEE-ST...T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 3 DgynWrKYGqKevkgsefprsYYrCtsa...gCpvkkkversaedpkvveitYegeHnhe 59 D ++WrKYGqK++ gs+fpr+YYrC+++ gC+++k+v+rs++d +++ itY +eHnh+ AT5G45260.1 1209 DLWTWRKYGQKDILGSRFPRGYYRCAYKfthGCKATKQVQRSETDSNMLAITYLSEHNHP 1268 99**********************99999**************************8 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF52200	3.92E-6	11	111	IPR000157	Toll/interleukin-1 receptor homology (TIR) domain
SuperFamily	SSF52540	2.33E-50	153	397	IPR027417	P-loop containing nucleoside triphosphate hydrolase
Pfam	PF00931	1.8E-23	170	407	IPR002182	NB-ARC
Gene3D	G3DSA:3.40.50.300	2.3E-10	172	288	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PRINTS	PR00364	1.8E-23	174	189	No hit	No description
PRINTS	PR00364	1.8E-23	243	257	No hit	No description
PRINTS	PR00364	1.8E-23	337	351	No hit	No description
SuperFamily	SSF46785	4.08E-11	374	475	IPR011991	Winged helix-turn-helix DNA-binding domain
Gene3D	G3DSA:3.80.10.10	1.2E-9	506	704	IPR032675	Leucine-rich repeat domain, L domain-like
SuperFamily	SSF52058	1.3E-26	512	851	IPR032675	Leucine-rich repeat domain, L domain-like
Pfam	PF07725	8.7E-9	577	596	IPR011713	Leucine-rich repeat 3
Gene3D	G3DSA:3.80.10.10	2.3E-18	705	868	IPR032675	Leucine-rich repeat domain, L domain-like
PRINTS	PR00364	1.8E-23	806	822	No hit	No description
SMART	SM00774	9.1E-36	1207	1269	IPR003657	WRKY domain
SuperFamily	SSF118290	5.89E-23	1207	1270	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	6.0E-25	1207	1270	IPR003657	WRKY domain
PROSITE profile	PS50811	25.488	1209	1270	IPR003657	WRKY domain
Pfam	PF03106	5.8E-23	1209	1268	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0007165	Biological Process	signal transduction
GO:0008219	Biological Process	cell death
GO:0009816	Biological Process	defense response to bacterium, incompatible interaction
GO:0005634	Cellular Component	nucleus
GO:0005737	Cellular Component	cytoplasm
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0005515	Molecular Function	protein binding
GO:0005524	Molecular Function	ATP binding
GO:0043531	Molecular Function	ADP binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0008019	anatomy	leaf lamina base
PO:0009005	anatomy	root
PO:0009006	anatomy	shoot system
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0025281	anatomy	pollen
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007064	developmental stage	LP.12 twelve leaves visible stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 1288 aa Download sequence Send to blast
MTNCEKDEEF VCISCVEEVR YSFVSHLSEA LRRKGINNVV VDVDIDDLLF KESQAKIEKA 60 GVSVMVLPGN CDPSEVWLDK FAKVLECQRN NKDQAVVSVL YGDSLLRDQW LSELDFRGLS 120 RIHQSRKECS DSILVEEIVR DVYETHFYVG RIGIYSKLLE IENMVNKQPI GIRCVGIWGM 180 PGIGKTTLAK AVFDQMSSAF DASCFIEDYD KSIHEKGLYC LLEEQLLPGN DATIMKLSSL 240 RDRLNSKRVL VVLDDVRNAL VGESFLEGFD WLGPGSLIII TSRDKQVFCL CGINQIYEVQ 300 GLNEKEARQL FLLSASIKED MGEQNLQELS VRVINYANGN PLAISVYGRE LKGKKKLSEM 360 ETAFLKLKRR PPFKIVDAFK STYDTLSDNE KNIFLDIACF FQGENVNYVI QLLEGCGFFP 420 HVEIDVLVDK CLVTISENRV WLHKLTQDIG REIINGETVQ IERRRRLWEP WSIKYLLEYN 480 EHKANGEPKT TFKRAQGSEE IEGLFLDTSN LRFDLQPSAF KNMLNLRLLK IYCSNPEVHP 540 VINFPTGSLH SLPNELRLLH WENYPLKSLP QNFDPRHLVE INMPYSQLQK LWGGTKNLEM 600 LRTIRLCHSH HLVDIDDLLK AENLEVIDLQ GCTRLQNFPA AGRLLRLRVV NLSGCIKIKS 660 VLEIPPNIEK LHLQGTGILA LPVSTVKPNH RELVNFLTEI PGLSEELERL TSLLESNSSC 720 QDLGKLICLE LKDCSCLQSL PNMANLDLNV LDLSGCSSLN SIQGFPRFLK QLYLGGTAIR 780 EVPQLPQSLE ILNAHGSCLR SLPNMANLEF LKVLDLSGCS ELETIQGFPR NLKELYFAGT 840 TLREVPQLPL SLEVLNAHGS DSEKLPMHYK FNNFFDLSQQ VVNDFLLKTL TYVKHIPRGY 900 TQELINKAPT FSFSAPSHTN QNATFDLQSG SSVMTRLNHS WRNTLVGFGM LVEVAFPEDY 960 CDATDVGISC VCRWSNKEGR SCRIERKFHC WAPWQVVPKV RKDHTFVFSD VNMRPSTGEG 1020 NDPDIWAGLV VFEFFPINQQ TKCLNDRFTV RRCGVRVINV ATGNTSLENI ALVLSLDPVE 1080 VSGYEVLRVS YDDLQEMDKV LFLYIASLFN DEDVDFVAPL IAGIDLDVSS GLKVLADVSL 1140 ISVSSNGEIV MHSLQRQMGK EILHGQSMLL SDCESSMTEN LSDVPKKKKK HSESRVKKVV 1200 SIPAIDEGDL WTWRKYGQKD ILGSRFPRGY YRCAYKFTHG CKATKQVQRS ETDSNMLAIT 1260 YLSEHNHPRP TKRKALADST RSTSSSIC

Sequence ? help Back to Top

Protein Sequence Length: 1288 aa Download sequence Send to blast

MTNCEKDEEF VCISCVEEVR YSFVSHLSEA LRRKGINNVV VDVDIDDLLF KESQAKIEKA  60
GVSVMVLPGN CDPSEVWLDK FAKVLECQRN NKDQAVVSVL YGDSLLRDQW LSELDFRGLS  120
RIHQSRKECS DSILVEEIVR DVYETHFYVG RIGIYSKLLE IENMVNKQPI GIRCVGIWGM  180
PGIGKTTLAK AVFDQMSSAF DASCFIEDYD KSIHEKGLYC LLEEQLLPGN DATIMKLSSL  240
RDRLNSKRVL VVLDDVRNAL VGESFLEGFD WLGPGSLIII TSRDKQVFCL CGINQIYEVQ  300
GLNEKEARQL FLLSASIKED MGEQNLQELS VRVINYANGN PLAISVYGRE LKGKKKLSEM  360
ETAFLKLKRR PPFKIVDAFK STYDTLSDNE KNIFLDIACF FQGENVNYVI QLLEGCGFFP  420
HVEIDVLVDK CLVTISENRV WLHKLTQDIG REIINGETVQ IERRRRLWEP WSIKYLLEYN  480
EHKANGEPKT TFKRAQGSEE IEGLFLDTSN LRFDLQPSAF KNMLNLRLLK IYCSNPEVHP  540
VINFPTGSLH SLPNELRLLH WENYPLKSLP QNFDPRHLVE INMPYSQLQK LWGGTKNLEM  600
LRTIRLCHSH HLVDIDDLLK AENLEVIDLQ GCTRLQNFPA AGRLLRLRVV NLSGCIKIKS  660
VLEIPPNIEK LHLQGTGILA LPVSTVKPNH RELVNFLTEI PGLSEELERL TSLLESNSSC  720
QDLGKLICLE LKDCSCLQSL PNMANLDLNV LDLSGCSSLN SIQGFPRFLK QLYLGGTAIR  780
EVPQLPQSLE ILNAHGSCLR SLPNMANLEF LKVLDLSGCS ELETIQGFPR NLKELYFAGT  840
TLREVPQLPL SLEVLNAHGS DSEKLPMHYK FNNFFDLSQQ VVNDFLLKTL TYVKHIPRGY  900
TQELINKAPT FSFSAPSHTN QNATFDLQSG SSVMTRLNHS WRNTLVGFGM LVEVAFPEDY  960
CDATDVGISC VCRWSNKEGR SCRIERKFHC WAPWQVVPKV RKDHTFVFSD VNMRPSTGEG  1020
NDPDIWAGLV VFEFFPINQQ TKCLNDRFTV RRCGVRVINV ATGNTSLENI ALVLSLDPVE  1080
VSGYEVLRVS YDDLQEMDKV LFLYIASLFN DEDVDFVAPL IAGIDLDVSS GLKVLADVSL  1140
ISVSSNGEIV MHSLQRQMGK EILHGQSMLL SDCESSMTEN LSDVPKKKKK HSESRVKKVV  1200
SIPAIDEGDL WTWRKYGQKD ILGSRFPRGY YRCAYKFTHG CKATKQVQRS ETDSNMLAIT  1260
YLSEHNHPRP TKRKALADST RSTSSSIC

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
4c6t_A	9e-95	6	153	4	151	PROBABLE WRKY TRANSCRIPTION FACTOR 52
4c6t_C	9e-95	6	153	4	151	PROBABLE WRKY TRANSCRIPTION FACTOR 52
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Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	1185	1197	KKKKKHSESRVKK

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.30021	0.0	root

Expression -- Microarray ? help Back to Top
Source	ID	E-value
Genevisible	248995_at	0.0
Expression Atlas	AT5G45260	-
AtGenExpress	AT5G45260	-
ATTED-II	AT5G45260	-

Functional Description ? help Back to Top
Source	Description
TAIR	Confers resistance to Ralstonia solanacearum. Similar to NBLS-TIR resistance genes,and also contains similarity to transcription factors. Interacts with pathogen effector protein AvrPop2.
UniProt	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Acts also as a disease resistance protein involved in resistance to fungal and bacterial pathogens, including R.solanacearum, P.syringae pv. tomato and C.higginsianum. Heterodimerization with RPS4 is required to form a functional complex to recognize AvrRps4 and PopP2 (PubMed:24744375). Contributes to temperature-conditioned RPS4 auto-immunity (PubMed:24146667). {ECO:0000269\|PubMed:10571887, ECO:0000269\|PubMed:11842188, ECO:0000269\|PubMed:19519800, ECO:0000269\|PubMed:24146667, ECO:0000269\|PubMed:24744375, ECO:0000305}.

Function -- GeneRIF ? help Back to Top
RRS1-Ws and RPS4-Ws, function cooperatively as a dual R-gene system against at least three distinct pathogens. [PMID: 19519800] Distinct allelic forms of RPS4 and RRS1 probably cooperate to confer resistance to different pathogens. [PMID: 19686535] A pair of Arabidopsis nucleotide binding and leucine rich repeat-type R genes, RPS4 and RRS1, properly function in two other Brassicaceae, Brassica rapa and Brassica napus, but also in two Solanaceae, Nicotiana benthamiana and tomato. [PMID: 23437080] properly function for pathogen resistance in two other Brassicaceae, Brassica rapa and B. napus, but also in two Solanaceae, Nicotiana benthamiana and tomato (Solanum lycopersicum) [PMID: 23518587] findings show RPS4 and RRS1 physically associate; TIR domain heterodimerization is required to form a functional RRS1/RPS4 effector recognition complex; RPS4 and RRS1 function as a receptor complex in which the 2 components play distinct roles in recognition and signaling [PMID: 24744375] We show that many sushi(slh1) mutants carry mutations in RPS4, suggesting that RPS4 acts downstream or in a complex with RRS1. Interestingly, several mutations were identified in a domain C-terminal to the RPS4 LRR domain [PMID: 25340333] Here, we report that dual R proteins, RRS1 and RPS4, from A. thaliana ecotype Wassilewskija confer resistance to bacterial wilt in transgenic Brassica crops. [PMID: 25763492] The Arabidopsis RRS1-R nucleotide-binding, leucine-rich repeat (NB-LRR) protein carries a C-terminal WRKY DNA binding domain and forms a receptor complex with RPS4, another NB-LRR protein. This complex detects the bacterial effectors AvrRps4 or PopP2 and then activates defense. [PMID: 26000484] Although quantitative disease resistance (QDR) to Xanthomonas campestris is conferred by At5g22540 encoding for a protein of unknown function, QDR to XccCFBP6943 involves the well-known immune receptor pair RRS1/RPS4. [PMID: 26212639] The RRS1-leucine zipper motif is crucial for the regulation of the RPS4/RRS1 complex. [PMID: 26750751] RRS1 domains responsible for response to bacterial effectors from plant pathogens. [PMID: 30254172]

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT5G45260.1

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT5G45260

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AK226456	0.0	AK226456.1 Arabidopsis thaliana mRNA for disease resistance like protein, complete cds, clone: RAFL06-15-E06.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_199339.2	0.0	Disease resistance protein (TIR-NBS-LRR class)
Swissprot	P0DKH5	0.0	WRK52_ARATH; Disease resistance protein RRS1
TrEMBL	L7Z7P4	0.0	L7Z7P4_ARATH; Resistance to ralstonia solanacearum 1 (Fragment)
STRING	AT5G45260.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM2506	8	14
Representative plant	OGRP18	2	3

Link Out ? help Back to Top
Phytozome	AT5G45260.1
Entrez Gene	834562
iHOP	AT5G45260
wikigenes	AT5G45260

Publications ? help Back to Top
Gassmann W,Hinsch ME,Staskawicz BJ The Arabidopsis RPS4 bacterial-resistance gene is a member of the TIR-NBS-LRR family of disease-resistance genes. Plant J., 1999. 20(3): p. 265-77 [PMID:10571887] Eulgem T,Rushton PJ,Robatzek S,Somssich IE The WRKY superfamily of plant transcription factors. Trends Plant Sci., 2000. 5(5): p. 199-206 [PMID:10785665] Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Deslandes L, et al. Resistance to Ralstonia solanacearum in Arabidopsis thaliana is conferred by the recessive RRS1-R gene, a member of a novel family of resistance genes. Proc. Natl. Acad. Sci. U.S.A., 2002. 99(4): p. 2404-9 [PMID:11842188] Seki M, et al. Functional annotation of a full-length Arabidopsis cDNA collection. Science, 2002. 296(5565): p. 141-5 [PMID:11910074] Lahaye T The Arabidopsis RRS1-R disease resistance gene--uncovering the plant's nucleus as the new battlefield of plant defense? Trends Plant Sci., 2002. 7(10): p. 425-7 [PMID:12399170] Deslandes L, et al. Physical interaction between RRS1-R, a protein conferring resistance to bacterial wilt, and PopP2, a type III effector targeted to the plant nucleus. Proc. Natl. Acad. Sci. U.S.A., 2003. 100(13): p. 8024-9 [PMID:12788974] Lahaye T Illuminating the molecular basis of gene-for-gene resistance; Arabidopsis thaliana RRS1-R and its interaction with Ralstonia solanacearum popP2. Trends Plant Sci., 2004. 9(1): p. 1-4 [PMID:14729210] Noutoshi Y, et al. A single amino acid insertion in the WRKY domain of the Arabidopsis TIR-NBS-LRR-WRKY-type disease resistance protein SLH1 (sensitive to low humidity 1) causes activation of defense responses and hypersensitive cell death. Plant J., 2005. 43(6): p. 873-88 [PMID:16146526] Hu J, et al. Transcriptional responses of Arabidopsis thaliana during wilt disease caused by the soil-borne phytopathogenic bacterium, Ralstonia solanacearum. PLoS ONE, 2008. 3(7): p. e2589 [PMID:18596930] Narusaka M, et al. RRS1 and RPS4 provide a dual Resistance-gene system against fungal and bacterial pathogens. Plant J., 2009. 60(2): p. 218-26 [PMID:19519800] Birker D, et al. A locus conferring resistance to Colletotrichum higginsianum is shared by four geographically distinct Arabidopsis accessions. Plant J., 2009. 60(4): p. 602-13 [PMID:19686535] Narusaka M,Kubo Y,Shiraishi T,Iwabuchi M,Narusaka Y A dual resistance gene system prevents infection by three distinct pathogens. Plant Signal Behav, 2009. 4(10): p. 954-5 [PMID:19826224] Yang H, et al. A mutant CHS3 protein with TIR-NB-LRR-LIM domains modulates growth, cell death and freezing tolerance in a temperature-dependent manner in Arabidopsis. Plant J., 2010. 63(2): p. 283-96 [PMID:20444230] Nemoto K, et al. Autophosphorylation profiling of Arabidopsis protein kinases using the cell-free system. Phytochemistry, 2011. 72(10): p. 1136-44 [PMID:21477822] Van der Linden L, et al. Gene-for-gene tolerance to bacterial wilt in Arabidopsis. Mol. Plant Microbe Interact., 2013. 26(4): p. 398-406 [PMID:23234403] Narusaka M, et al. Interfamily transfer of dual NB-LRR genes confers resistance to multiple pathogens. PLoS ONE, 2013. 8(2): p. e55954 [PMID:23437080] Narusaka M, et al. Breaking restricted taxonomic functionality by dual resistance genes. Plant Signal Behav, 2013. 8(6): p. e24244 [PMID:23518587] Heidrich K, et al. Arabidopsis TNL-WRKY domain receptor RRS1 contributes to temperature-conditioned RPS4 auto-immunity. Front Plant Sci, 2013. 4: p. 403 [PMID:24146667] Williams SJ, et al. Structural basis for assembly and function of a heterodimeric plant immune receptor. Science, 2014. 344(6181): p. 299-303 [PMID:24744375] Sohn KH, et al. The nuclear immune receptor RPS4 is required for RRS1SLH1-dependent constitutive defense activation in Arabidopsis thaliana. PLoS Genet., 2014. 10(10): p. e1004655 [PMID:25340333] Cesari S,Bernoux M,Moncuquet P,Kroj T,Dodds PN A novel conserved mechanism for plant NLR protein pairs: the "integrated decoy" hypothesis. Front Plant Sci, 2014. 5: p. 606 [PMID:25506347] Narusaka M,Hatakeyama K,Shirasu K,Narusaka Y Arabidopsis dual resistance proteins, both RPS4 and RRS1, are required for resistance to bacterial wilt in transgenic Brassica crops. Plant Signal Behav, 2014. 9(7): p. e29130 [PMID:25763492] Sarris PF, et al. A Plant Immune Receptor Detects Pathogen Effectors that Target WRKY Transcription Factors. Cell, 2015. 161(5): p. 1089-100 [PMID:26000484] Debieu M,Huard-Chauveau C,Genissel A,Roux F,Roby D Quantitative disease resistance to the bacterial pathogen Xanthomonas campestris involves an Arabidopsis immune receptor pair and a gene of unknown function. Mol. Plant Pathol., 2016. 17(4): p. 510-20 [PMID:26212639] Narusaka M, et al. Leucine zipper motif in RRS1 is crucial for the regulation of Arabidopsis dual resistance protein complex RPS4/RRS1. Sci Rep, 2016. 6: p. 18702 [PMID:26750751] Ma Y, et al. Distinct modes of derepression of an Arabidopsis immune receptor complex by two different bacterial effectors. Proc. Natl. Acad. Sci. U.S.A., 2018. 115(41): p. 10218-10227 [PMID:30254172] Deslandes L, et al. Genetic characterization of RRS1, a recessive locus in Arabidopsis thaliana that confers resistance to the bacterial soilborne pathogen Ralstonia solanacearum. Mol. Plant Microbe Interact., 1998. 11(7): p. 659-67 [PMID:9650298]