PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT2G25000.1

Common Name

ATWRKY60, F27C12.8, WRKY60

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

WRKY

Protein Properties

Length: 271aa MW: 30577.6 Da PI: 9.5691

Description

WRKY DNA-binding protein 60

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT2G25000.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	93	2.1e-29	145	204	1	59
---SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 1 ldDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 ++Dgy+WrKYGqK ++++ pr+Y+rC+++ +C vkkkv+rsaedp++++ tYeg+Hnh+ AT2G25000.1 145 VKDGYQWRKYGQKITRDNPSPRAYFRCSFSpSCLVKKKVQRSAEDPSFLVATYEGTHNHT 204 58*************************99**************************6 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	1.4E-27	136	206	IPR003657	WRKY domain
SuperFamily	SSF118290	6.41E-25	142	206	IPR003657	WRKY domain
SMART	SM00774	2.0E-32	145	205	IPR003657	WRKY domain
Pfam	PF03106	8.3E-24	146	203	IPR003657	WRKY domain
PROSITE profile	PS50811	26.234	147	206	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0002237	Biological Process	response to molecule of bacterial origin
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009751	Biological Process	response to salicylic acid
GO:0031347	Biological Process	regulation of defense response
GO:0042742	Biological Process	defense response to bacterium
GO:0050832	Biological Process	defense response to fungus
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0005515	Molecular Function	protein binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0008019	anatomy	leaf lamina base
PO:0009005	anatomy	root
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0025034	anatomy	leaf
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 271 aa Download sequence Send to blast
MDYDPNTNPF DLHFSGKLPK REVSASASKV VEKKWLVKDE KRNMLQDEIN RVNSENKKLT 60 EMLARVCEKY YALNNLMEEL QSRKSPESVN FQNKQLTGKR KQELDEFVSS PIGLSLGPIE 120 NITNDKATVS TAYFAAEKSD TSLTVKDGYQ WRKYGQKITR DNPSPRAYFR CSFSPSCLVK 180 KKVQRSAEDP SFLVATYEGT HNHTGPHASV SRTVKLDLVQ GGLEPVEEKK ERGTIQEVLV 240 QQMASSLTKD PKFTAALATA ISGRLIEHSR T

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wj2_A	5e-20	147	206	19	77	Probable WRKY transcription factor 4
2lex_A	5e-20	147	206	19	77	Probable WRKY transcription factor 4
Search in ModeBase

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.28320	0.0	leaf\| root

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	186502859	0.0
Genevisible	263536_at	0.0
Expression Atlas	AT2G25000	-
AtGenExpress	AT2G25000	-
ATTED-II	AT2G25000	-

Functional Description ? help Back to Top
Source	Description
TAIR	Pathogen-induced transcription factor. Forms protein complexes with itself and with WRKY40. Coexpression with WRKY18 or WRKY40 made plants more susceptible to both P. syringae and B. cinerea. WRKY18, WRKY40, and WRKY60 have partially redundant roles in response to the hemibiotrophic bacterial pathogen Pseudomonas syringae and the necrotrophic fungal pathogen Botrytis cinerea, with WRKY18 playing a more important role than the other two.
UniProt	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). {ECO:0000250}.

Function -- GeneRIF ? help Back to Top
WRKY60 interacts both physically and functionally with WRKY40 and WRKY18 in a complex pattern of overlapping, antagonistic, and distinct roles in plant responses to different types of microbial pathogens. [PMID: 16603654] Data suggest that WRKY18, WRKY40 and WRKY60 form a highly interacting regulatory network that modulates gene expression in both plant defense and stress responses by acting as either transcription activator or repressor. [PMID: 21167067]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00068	PBM	25215497	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT2G25000.1

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- Hormone ? help Back to Top
Source	Hormone
AHD	salicylic acid

Interaction ? help Back to Top
Source	Intact With
BioGRID	AT2G25000, AT4G31800, AT1G69810, AT1G80840
IntAct	Search Q9SK33

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT2G25000

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AK227987	0.0	AK227987.1 Arabidopsis thaliana mRNA for putative WRKY-type DNA binding protein, complete cds, clone: RAFL14-52-F05.
GenBank	BT004611	0.0	BT004611.1 Arabidopsis thaliana At2g25000 gene, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_180072.1	0.0	WRKY DNA-binding protein 60
Swissprot	Q9SK33	0.0	WRK60_ARATH; Probable WRKY transcription factor 60
TrEMBL	Q0WSE5	0.0	Q0WSE5_ARATH; Putative WRKY-type DNA binding protein
STRING	AT2G25000.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM6584	19	45
Representative plant	OGRP14	17	875

Link Out ? help Back to Top
Phytozome	AT2G25000.1
Entrez Gene	817039
iHOP	AT2G25000
wikigenes	AT2G25000

Publications ? help Back to Top
Eulgem T,Rushton PJ,Robatzek S,Somssich IE The WRKY superfamily of plant transcription factors. Trends Plant Sci., 2000. 5(5): p. 199-206 [PMID:10785665] Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Chen C,Chen Z Potentiation of developmentally regulated plant defense response by AtWRKY18, a pathogen-induced Arabidopsis transcription factor. Plant Physiol., 2002. 129(2): p. 706-16 [PMID:12068113] Dong J,Chen C,Chen Z Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response. Plant Mol. Biol., 2003. 51(1): p. 21-37 [PMID:12602888] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Hoth S, et al. Monitoring genome-wide changes in gene expression in response to endogenous cytokinin reveals targets in Arabidopsis thaliana. FEBS Lett., 2003. 554(3): p. 373-80 [PMID:14623097] Li J,Brader G,Palva ET The WRKY70 transcription factor: a node of convergence for jasmonate-mediated and salicylate-mediated signals in plant defense. Plant Cell, 2004. 16(2): p. 319-31 [PMID:14742872] Goda H, et al. Comprehensive comparison of auxin-regulated and brassinosteroid-regulated genes in Arabidopsis. Plant Physiol., 2004. 134(4): p. 1555-73 [PMID:15047898] Ma S,Quist TM,Ulanov A,Joly R,Bohnert HJ Loss of TIP1;1 aquaporin in Arabidopsis leads to cell and plant death. Plant J., 2004. 40(6): p. 845-59 [PMID:15584951] Nagata T,Yamada H,Du Z,Todoriki S,Kikuchi S Microarray analysis of genes that respond to gamma-irradiation in Arabidopsis. J. Agric. Food Chem., 2005. 53(4): p. 1022-30 [PMID:15713015] Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Truman W,de Zabala MT,Grant M Type III effectors orchestrate a complex interplay between transcriptional networks to modify basal defence responses during pathogenesis and resistance. Plant J., 2006. 46(1): p. 14-33 [PMID:16553893] Xu X,Chen C,Fan B,Chen Z Physical and functional interactions between pathogen-induced Arabidopsis WRKY18, WRKY40, and WRKY60 transcription factors. Plant Cell, 2006. 18(5): p. 1310-26 [PMID:16603654] Tosti N, et al. Gene expression profiles of O3-treated Arabidopsis plants. Plant Cell Environ., 2006. 29(9): p. 1686-702 [PMID:16913859] Ascencio-Ib Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection. Plant Physiol., 2008. 148(1): p. 436-54 [PMID:18650403] Peng Y, et al. OsWRKY62 is a negative regulator of basal and Xa21-mediated defense against Xanthomonas oryzae pv. oryzae in rice. Mol Plant, 2008. 1(3): p. 446-58 [PMID:19825552] Shang Y, et al. The Mg-chelatase H subunit of Arabidopsis antagonizes a group of WRKY transcription repressors to relieve ABA-responsive genes of inhibition. Plant Cell, 2010. 22(6): p. 1909-35 [PMID:20543028] Chen H, et al. Roles of arabidopsis WRKY18, WRKY40 and WRKY60 transcription factors in plant responses to abscisic acid and abiotic stress. BMC Plant Biol., 2010. 10: p. 281 [PMID:21167067] Arabidopsis Interactome Mapping Consortium Evidence for network evolution in an Arabidopsis interactome map. Science, 2011. 333(6042): p. 601-7 [PMID:21798944] Wenke K, et al. Volatiles of two growth-inhibiting rhizobacteria commonly engage AtWRKY18 function. Plant J., 2012. 70(3): p. 445-59 [PMID:22188129] Liu ZQ, et al. Cooperation of three WRKY-domain transcription factors WRKY18, WRKY40, and WRKY60 in repressing two ABA-responsive genes ABI4 and ABI5 in Arabidopsis. J. Exp. Bot., 2012. 63(18): p. 6371-92 [PMID:23095997] Koyama T, et al. A regulatory cascade involving class II ETHYLENE RESPONSE FACTOR transcriptional repressors operates in the progression of leaf senescence. Plant Physiol., 2013. 162(2): p. 991-1005 [PMID:23629833] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Zhang Y, et al. Regulation of oncogene expression in T-DNA-transformed host plant cells. PLoS Pathog., 2015. 11(1): p. e1004620 [PMID:25615824] Geilen K,Böhmer M Dynamic subnuclear relocalisation of WRKY40 in response to Abscisic acid in Arabidopsis thaliana. Sci Rep, 2015. 5: p. 13369 [PMID:26293691] Geilen K,Böhmer M Dynamic subnuclear relocalization of WRKY40, a potential new mechanism of ABA-dependent transcription factor regulation. Plant Signal Behav, 2015. 10(11): p. e1106659 [PMID:26479147] Lu K, et al. Overexpression of an Arabidopsis cysteine-rich receptor-like protein kinase, CRK5, enhances abscisic acid sensitivity and confers drought tolerance. J. Exp. Bot., 2016. 67(17): p. 5009-27 [PMID:27406784]