PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Lupinus angustifolius
ZF-HD Family
Species TF ID Description
OIV90183ZF-HD family protein
OIV90257ZF-HD family protein
OIV92709ZF-HD family protein
OIV93044ZF-HD family protein
OIV93391ZF-HD family protein
OIV94167ZF-HD family protein
OIV94489ZF-HD family protein
OIV94797ZF-HD family protein
OIV96583ZF-HD family protein
OIV97098ZF-HD family protein
OIV97866ZF-HD family protein
OIV99995ZF-HD family protein
OIV99996ZF-HD family protein
OIW04302ZF-HD family protein
OIW04756ZF-HD family protein
OIW06842ZF-HD family protein
OIW10290ZF-HD family protein
OIW10603ZF-HD family protein
OIW11007ZF-HD family protein
OIW12450ZF-HD family protein
OIW12451ZF-HD family protein
OIW18424ZF-HD family protein
OIW20178ZF-HD family protein
OIW20770ZF-HD family protein
OIW21156ZF-HD family protein
OIW21157ZF-HD family protein
OIW21499ZF-HD family protein
OIW21718ZF-HD family protein
OIW21846ZF-HD family protein
ZF-HD Family Introduction

This group of sequences described by a 54-residue domain found in the N-terminal region of plant proteins, the vast majority of which contain a ZF-HD class homeobox domain toward the C terminus. The region between the two domains typically is rich in low complexity sequence. The companion ZF-HD homeobox domain is described in INTERPRO:IPR006455.

A one-hybrid screen resulted in the cloning of four different members of a novel class of plant homeodomain proteins, which are most likely involved in the mesophyll-specific expression of the C4 PEPCase gene in C4 species of the genus Flaveria. Inspection of the homeodomains of the four proteins reveals that they share many common features with homeodomains described so far, but there are also significant differences. Interestingly, this class of homeodomain proteins occurs also in Arabidopsis thaliana and other C3 plants. One-hybrid experiments as well as in vitro DNA binding studies confirmed that these novel homeodomain proteins specifically interact with the proximal region of the C4 PEPCase (C4 phosphoenolpyruvate carboxylase) gene. The N-terminal domains of the homeodomain proteins contain highly conserved sequence motifs. Two-hybrid experiments show that these motifs are sufficient to confer homo- or heterodimer formation between the proteins. Mutagenesis of conserved cysteine residues within the dimerization domain indicates that these residues are essential for dimer formation. Therefore, we designate this novel class of homeobox proteins ZF-HD, for zinc finger homeodomain protein. Our data suggest that the ZF-HD class of homeodomain proteins may be involved in the establishment of the characteristic expression pattern of the C4 PEPCase gene.

Windhovel A, Hein I, Dabrowa R, Stockhaus J.
Characterization of a novel class of plant homeodomain proteins that bind to the C4 phosphoenolpyruvate carboxylase gene of Flaveria trinervia.
Plant Mol Biol. 2001 Jan;45(2):201-14.
PMID: 11289511