PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Lupinus angustifolius
HSF Family
Species TF ID Description
OIV90363HSF family protein
OIV92314HSF family protein
OIV92878HSF family protein
OIV92880HSF family protein
OIV92990HSF family protein
OIV93445HSF family protein
OIV94895HSF family protein
OIV96382HSF family protein
OIV96409HSF family protein
OIV97384HSF family protein
OIV99751HSF family protein
OIW00528HSF family protein
OIW02805HSF family protein
OIW03677HSF family protein
OIW04999HSF family protein
OIW08011HSF family protein
OIW08797HSF family protein
OIW09555HSF family protein
OIW09704HSF family protein
OIW10384HSF family protein
OIW10411HSF family protein
OIW10956HSF family protein
OIW11280HSF family protein
OIW14871HSF family protein
OIW17948HSF family protein
OIW17999HSF family protein
OIW18724HSF family protein
OIW19179HSF family protein
OIW19253HSF family protein
OIW19794HSF family protein
OIW20191HSF family protein
OIW21201HSF family protein
OIW21922HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645