PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Chenopodium quinoa
HSF Family
Species TF ID Description
AUR62000210-RAHSF family protein
AUR62001930-RAHSF family protein
AUR62002076-RAHSF family protein
AUR62003880-RAHSF family protein
AUR62006556-RAHSF family protein
AUR62007327-RAHSF family protein
AUR62009126-RAHSF family protein
AUR62010631-RAHSF family protein
AUR62010997-RAHSF family protein
AUR62011973-RAHSF family protein
AUR62016009-RAHSF family protein
AUR62017275-RAHSF family protein
AUR62018547-RAHSF family protein
AUR62018674-RAHSF family protein
AUR62020872-RAHSF family protein
AUR62021216-RAHSF family protein
AUR62021507-RAHSF family protein
AUR62024462-RAHSF family protein
AUR62026052-RAHSF family protein
AUR62028193-RAHSF family protein
AUR62029043-RAHSF family protein
AUR62032284-RAHSF family protein
AUR62033579-RAHSF family protein
AUR62033827-RAHSF family protein
AUR62033941-RAHSF family protein
AUR62035755-RAHSF family protein
AUR62039129-RAHSF family protein
AUR62039492-RAHSF family protein
AUR62041419-RAHSF family protein
AUR62041808-RAHSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645