PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Cucurbita pepo subsp. pepo
HSF Family
Species TF ID Description
XP_023511700.1HSF family protein
XP_023511701.1HSF family protein
XP_023516316.1HSF family protein
XP_023516469.1HSF family protein
XP_023517960.1HSF family protein
XP_023518612.1HSF family protein
XP_023518714.1HSF family protein
XP_023519250.1HSF family protein
XP_023519251.1HSF family protein
XP_023521033.1HSF family protein
XP_023521234.1HSF family protein
XP_023521235.1HSF family protein
XP_023521303.1HSF family protein
XP_023525267.1HSF family protein
XP_023526227.1HSF family protein
XP_023526228.1HSF family protein
XP_023526229.1HSF family protein
XP_023526230.1HSF family protein
XP_023527179.1HSF family protein
XP_023527625.1HSF family protein
XP_023530778.1HSF family protein
XP_023531680.1HSF family protein
XP_023534238.1HSF family protein
XP_023534265.1HSF family protein
XP_023534267.1HSF family protein
XP_023536181.1HSF family protein
XP_023536624.1HSF family protein
XP_023537299.1HSF family protein
XP_023537300.1HSF family protein
XP_023537301.1HSF family protein
XP_023537302.1HSF family protein
XP_023539565.1HSF family protein
XP_023539978.1HSF family protein
XP_023540187.1HSF family protein
XP_023540223.1HSF family protein
XP_023540455.1HSF family protein
XP_023541399.1HSF family protein
XP_023543806.1HSF family protein
XP_023543962.1HSF family protein
XP_023543963.1HSF family protein
XP_023544792.1HSF family protein
XP_023545868.1HSF family protein
XP_023545907.1HSF family protein
XP_023546638.1HSF family protein
XP_023547324.1HSF family protein
XP_023549067.1HSF family protein
XP_023549068.1HSF family protein
XP_023549069.1HSF family protein
XP_023549071.1HSF family protein
XP_023551563.1HSF family protein
XP_023552500.1HSF family protein
XP_023552557.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645