PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Cynara cardunculus var. scolymus
HSF Family
Species TF ID Description
XP_024960526.1HSF family protein
XP_024961175.1HSF family protein
XP_024962257.1HSF family protein
XP_024967378.1HSF family protein
XP_024967379.1HSF family protein
XP_024967413.1HSF family protein
XP_024970812.1HSF family protein
XP_024970820.1HSF family protein
XP_024971474.1HSF family protein
XP_024973044.1HSF family protein
XP_024975853.1HSF family protein
XP_024977721.1HSF family protein
XP_024980312.1HSF family protein
XP_024980374.1HSF family protein
XP_024982072.1HSF family protein
XP_024982073.1HSF family protein
XP_024983900.1HSF family protein
XP_024984431.1HSF family protein
XP_024986102.1HSF family protein
XP_024988185.1HSF family protein
XP_024988186.1HSF family protein
XP_024989082.1HSF family protein
XP_024989083.1HSF family protein
XP_024989084.1HSF family protein
XP_024989085.1HSF family protein
XP_024989086.1HSF family protein
XP_024989167.1HSF family protein
XP_024991497.1HSF family protein
XP_024991498.1HSF family protein
XP_024992766.1HSF family protein
XP_024994351.1HSF family protein
XP_024994799.1HSF family protein
XP_024994800.1HSF family protein
XP_024994851.1HSF family protein
XP_024994852.1HSF family protein
XP_024994853.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645