PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Corchorus capsularis
FAR1 Family
Species TF ID Description
OMO49430FAR1 family protein
OMO50919FAR1 family protein
OMO51030FAR1 family protein
OMO52507FAR1 family protein
OMO52911FAR1 family protein
OMO52913FAR1 family protein
OMO52914FAR1 family protein
OMO53579FAR1 family protein
OMO53811FAR1 family protein
OMO54390FAR1 family protein
OMO54391FAR1 family protein
OMO57078FAR1 family protein
OMO58742FAR1 family protein
OMO60786FAR1 family protein
OMO60789FAR1 family protein
OMO61645FAR1 family protein
OMO62693FAR1 family protein
OMO65299FAR1 family protein
OMO65313FAR1 family protein
OMO65875FAR1 family protein
OMO65897FAR1 family protein
OMO67527FAR1 family protein
OMO68007FAR1 family protein
OMO68557FAR1 family protein
OMO69242FAR1 family protein
OMO71146FAR1 family protein
OMO72119FAR1 family protein
OMO74953FAR1 family protein
OMO75987FAR1 family protein
OMO76974FAR1 family protein
OMO81532FAR1 family protein
OMO83860FAR1 family protein
OMO86021FAR1 family protein
OMO87158FAR1 family protein
OMO92166FAR1 family protein
OMO93882FAR1 family protein
OMO94585FAR1 family protein
OMO96148FAR1 family protein
OMO96662FAR1 family protein
OMO96784FAR1 family protein
OMO98813FAR1 family protein
OMP00951FAR1 family protein
OMP01039FAR1 family protein
OMP04680FAR1 family protein
OMP12084FAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885