PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Abrus precatorius
HSF Family
Species TF ID Description
XP_027332454.1HSF family protein
XP_027332966.1HSF family protein
XP_027333145.1HSF family protein
XP_027336063.1HSF family protein
XP_027338353.1HSF family protein
XP_027342311.1HSF family protein
XP_027342484.1HSF family protein
XP_027342485.1HSF family protein
XP_027344040.1HSF family protein
XP_027346859.1HSF family protein
XP_027347295.1HSF family protein
XP_027348353.1HSF family protein
XP_027349202.1HSF family protein
XP_027350389.1HSF family protein
XP_027353090.1HSF family protein
XP_027355079.1HSF family protein
XP_027356466.1HSF family protein
XP_027358330.1HSF family protein
XP_027358572.1HSF family protein
XP_027359864.1HSF family protein
XP_027360056.1HSF family protein
XP_027360110.1HSF family protein
XP_027360112.1HSF family protein
XP_027360113.1HSF family protein
XP_027360114.1HSF family protein
XP_027361035.1HSF family protein
XP_027363442.1HSF family protein
XP_027364390.1HSF family protein
XP_027365101.1HSF family protein
XP_027365102.1HSF family protein
XP_027365103.1HSF family protein
XP_027365104.1HSF family protein
XP_027365105.1HSF family protein
XP_027365106.1HSF family protein
XP_027366097.1HSF family protein
XP_027366240.1HSF family protein
XP_027366868.1HSF family protein
XP_027367462.1HSF family protein
XP_027368557.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645